Marichromatium purpuratum 984

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Marichromatium; Marichromatium purpuratum

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3249 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W0E7P4|W0E7P4_MARPU Uncharacterized protein OS=Marichromatium purpuratum 984 OX=765910 GN=MARPU_10805 PE=4 SV=1
MM1 pKa = 7.75DD2 pKa = 3.93VSRR5 pKa = 11.84LTLCSLLVAAALAPHH20 pKa = 7.08AASADD25 pKa = 3.14DD26 pKa = 3.71WQRR29 pKa = 11.84IDD31 pKa = 3.24AAAPFVDD38 pKa = 3.23ADD40 pKa = 3.94GVFRR44 pKa = 11.84TPACSGSPRR53 pKa = 11.84LEE55 pKa = 4.19EE56 pKa = 4.58GEE58 pKa = 4.6VVATDD63 pKa = 2.95TDD65 pKa = 3.95YY66 pKa = 11.97AFFVRR71 pKa = 11.84DD72 pKa = 3.58GDD74 pKa = 4.01PEE76 pKa = 4.17RR77 pKa = 11.84LLLFFDD83 pKa = 4.89GGGACWDD90 pKa = 3.76AGTCIDD96 pKa = 4.7AALDD100 pKa = 3.76GNVLYY105 pKa = 10.39FPEE108 pKa = 4.21VDD110 pKa = 3.46EE111 pKa = 5.27TPDD114 pKa = 3.22EE115 pKa = 4.16LAGFGGVFDD124 pKa = 6.42LDD126 pKa = 3.61RR127 pKa = 11.84TDD129 pKa = 3.72NPLRR133 pKa = 11.84DD134 pKa = 3.83HH135 pKa = 6.39LQVYY139 pKa = 9.74LPYY142 pKa = 9.94CTGDD146 pKa = 3.31LHH148 pKa = 8.36LGARR152 pKa = 11.84DD153 pKa = 3.51TSYY156 pKa = 10.97PLPDD160 pKa = 4.12GGDD163 pKa = 2.85WTIYY167 pKa = 10.27HH168 pKa = 7.09RR169 pKa = 11.84GHH171 pKa = 7.16DD172 pKa = 3.63NVIAVLEE179 pKa = 4.04YY180 pKa = 10.86LADD183 pKa = 3.74YY184 pKa = 9.25YY185 pKa = 11.46ANEE188 pKa = 4.17VGEE191 pKa = 4.21PPEE194 pKa = 4.51EE195 pKa = 3.95IVLVGASAGGYY206 pKa = 8.7GVLYY210 pKa = 10.06NYY212 pKa = 7.96PALARR217 pKa = 11.84RR218 pKa = 11.84FPGVEE223 pKa = 4.11RR224 pKa = 11.84IRR226 pKa = 11.84VLVDD230 pKa = 2.9SSNGVISEE238 pKa = 4.32DD239 pKa = 3.26LFEE242 pKa = 4.96RR243 pKa = 11.84ALEE246 pKa = 4.17ADD248 pKa = 4.42GVWGAQEE255 pKa = 4.17NLDD258 pKa = 4.19PEE260 pKa = 4.32IASAFDD266 pKa = 4.01DD267 pKa = 4.58GAGQLVPGLFNTLGWQYY284 pKa = 10.78PDD286 pKa = 3.77ARR288 pKa = 11.84FGQYY292 pKa = 7.83TRR294 pKa = 11.84AYY296 pKa = 10.3DD297 pKa = 3.53AVQVLFFNVSSNLGNPEE314 pKa = 3.56RR315 pKa = 11.84WFDD318 pKa = 3.7PVYY321 pKa = 11.03LLLSGLEE328 pKa = 3.78WTTRR332 pKa = 11.84ARR334 pKa = 11.84ASMWGTAFTTWNYY347 pKa = 10.55RR348 pKa = 11.84FYY350 pKa = 10.93LAQGQTHH357 pKa = 5.58MVSFYY362 pKa = 11.34DD363 pKa = 4.34DD364 pKa = 4.16FFNEE368 pKa = 4.21DD369 pKa = 3.19SAGGIEE375 pKa = 4.05LSDD378 pKa = 3.33WFGDD382 pKa = 3.59MIEE385 pKa = 4.28RR386 pKa = 11.84RR387 pKa = 11.84WTFGSDD393 pKa = 2.33WRR395 pKa = 11.84NASCVPACLPPEE407 pKa = 4.25GLDD410 pKa = 4.39LSTLL414 pKa = 3.72

Molecular weight: 45.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W0E3D9|W0E3D9_MARPU Diguanylate cyclase OS=Marichromatium purpuratum 984 OX=765910 GN=MARPU_07445 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LKK11 pKa = 10.48RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.93GFRR19 pKa = 11.84ARR21 pKa = 11.84SATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 9.2VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.29GRR39 pKa = 11.84ARR41 pKa = 11.84LALL44 pKa = 3.93

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3249

0

3249

1091096

30

1974

335.8

36.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.579 ± 0.06

1.023 ± 0.016

5.779 ± 0.035

6.605 ± 0.044

3.164 ± 0.027

8.258 ± 0.038

2.384 ± 0.026

4.439 ± 0.03

1.926 ± 0.027

11.958 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.042 ± 0.019

2.057 ± 0.023

5.368 ± 0.037

3.451 ± 0.03

8.681 ± 0.052

4.611 ± 0.032

4.889 ± 0.033

7.197 ± 0.036

1.391 ± 0.02

2.196 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski