Polaromonas sp. CF318

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Polaromonas; unclassified Polaromonas

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4751 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J2LIP6|J2LIP6_9BURK Peptidase_S8 domain-containing protein OS=Polaromonas sp. CF318 OX=1144318 GN=PMI15_01949 PE=4 SV=1
MM1 pKa = 7.25SAVAEE6 pKa = 4.37NIQTDD11 pKa = 3.77MPAPFVFTDD20 pKa = 3.79SAASKK25 pKa = 9.65VAEE28 pKa = 4.86LIAEE32 pKa = 4.35EE33 pKa = 4.62GNPDD37 pKa = 2.99LKK39 pKa = 11.13LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.94GFTFDD59 pKa = 4.81EE60 pKa = 4.64ITNEE64 pKa = 4.77DD65 pKa = 4.2DD66 pKa = 3.25TTMTKK71 pKa = 10.66NGVSLLIDD79 pKa = 3.54AMSYY83 pKa = 10.32QYY85 pKa = 11.5LVGAEE90 pKa = 4.01IDD92 pKa = 3.89YY93 pKa = 11.28KK94 pKa = 11.39DD95 pKa = 4.47DD96 pKa = 4.08LEE98 pKa = 4.56GAQFVIKK105 pKa = 10.53NPNATSTCGCGSSFSAA121 pKa = 4.79

Molecular weight: 12.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J3DG70|J3DG70_9BURK Putative acyl-CoA transferase/carnitine dehydratase OS=Polaromonas sp. CF318 OX=1144318 GN=PMI15_00117 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.31QPSKK9 pKa = 8.92VKK11 pKa = 10.35RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 6.27GFLTRR21 pKa = 11.84MKK23 pKa = 9.36TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.8GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4751

0

4751

1515684

21

3673

319.0

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.979 ± 0.051

0.894 ± 0.012

4.904 ± 0.03

5.172 ± 0.034

3.598 ± 0.022

8.549 ± 0.044

2.089 ± 0.02

4.322 ± 0.023

3.82 ± 0.031

10.736 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.55 ± 0.018

2.778 ± 0.028

5.285 ± 0.026

3.931 ± 0.019

6.292 ± 0.035

5.588 ± 0.029

5.199 ± 0.041

7.576 ± 0.032

1.435 ± 0.017

2.303 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski