Amino acid dipepetide frequency for Polaromonas sp. CF318

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.682AlaAla: 18.682 ± 0.142
1.279AlaCys: 1.279 ± 0.032
6.166AlaAsp: 6.166 ± 0.077
6.374AlaGlu: 6.374 ± 0.073
4.113AlaPhe: 4.113 ± 0.055
12.117AlaGly: 12.117 ± 0.13
2.497AlaHis: 2.497 ± 0.047
5.09AlaIle: 5.09 ± 0.07
4.528AlaLys: 4.528 ± 0.069
14.726AlaLeu: 14.726 ± 0.132
3.761AlaMet: 3.761 ± 0.049
3.004AlaAsn: 3.004 ± 0.061
6.274AlaPro: 6.274 ± 0.09
5.974AlaGln: 5.974 ± 0.072
8.203AlaArg: 8.203 ± 0.085
7.074AlaSer: 7.074 ± 0.083
6.025AlaThr: 6.025 ± 0.077
9.317AlaVal: 9.317 ± 0.085
1.935AlaTrp: 1.935 ± 0.038
2.654AlaTyr: 2.654 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
1.139CysAla: 1.139 ± 0.029
0.113CysCys: 0.113 ± 0.008
0.478CysAsp: 0.478 ± 0.019
0.527CysGlu: 0.527 ± 0.02
0.305CysPhe: 0.305 ± 0.016
0.93CysGly: 0.93 ± 0.027
0.261CysHis: 0.261 ± 0.013
0.424CysIle: 0.424 ± 0.018
0.269CysLys: 0.269 ± 0.014
0.838CysLeu: 0.838 ± 0.025
0.211CysMet: 0.211 ± 0.011
0.238CysAsn: 0.238 ± 0.013
0.432CysPro: 0.432 ± 0.019
0.226CysGln: 0.226 ± 0.012
0.517CysArg: 0.517 ± 0.018
0.501CysSer: 0.501 ± 0.019
0.482CysThr: 0.482 ± 0.02
0.703CysVal: 0.703 ± 0.021
0.129CysTrp: 0.129 ± 0.01
0.215CysTyr: 0.215 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
6.469AspAla: 6.469 ± 0.075
0.444AspCys: 0.444 ± 0.018
2.202AspAsp: 2.202 ± 0.043
2.924AspGlu: 2.924 ± 0.052
2.064AspPhe: 2.064 ± 0.042
4.265AspGly: 4.265 ± 0.066
0.941AspHis: 0.941 ± 0.027
2.48AspIle: 2.48 ± 0.043
1.969AspLys: 1.969 ± 0.041
4.884AspLeu: 4.884 ± 0.067
1.25AspMet: 1.25 ± 0.032
1.285AspAsn: 1.285 ± 0.029
2.591AspPro: 2.591 ± 0.04
1.444AspGln: 1.444 ± 0.029
2.755AspArg: 2.755 ± 0.043
2.447AspSer: 2.447 ± 0.055
2.522AspThr: 2.522 ± 0.041
3.785AspVal: 3.785 ± 0.061
0.903AspTrp: 0.903 ± 0.022
1.418AspTyr: 1.418 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
6.763GluAla: 6.763 ± 0.077
0.376GluCys: 0.376 ± 0.018
2.13GluAsp: 2.13 ± 0.037
2.505GluGlu: 2.505 ± 0.046
1.929GluPhe: 1.929 ± 0.039
3.783GluGly: 3.783 ± 0.052
1.207GluHis: 1.207 ± 0.03
2.665GluIle: 2.665 ± 0.046
2.315GluLys: 2.315 ± 0.044
5.906GluLeu: 5.906 ± 0.069
1.304GluMet: 1.304 ± 0.033
1.32GluAsn: 1.32 ± 0.033
2.386GluPro: 2.386 ± 0.037
2.398GluGln: 2.398 ± 0.048
3.981GluArg: 3.981 ± 0.059
2.598GluSer: 2.598 ± 0.047
2.513GluThr: 2.513 ± 0.046
3.945GluVal: 3.945 ± 0.081
0.681GluTrp: 0.681 ± 0.024
1.012GluTyr: 1.012 ± 0.029
0.0GluXaa: 0.0 ± 0.0
Phe
4.3PheAla: 4.3 ± 0.056
0.394PheCys: 0.394 ± 0.017
2.331PheAsp: 2.331 ± 0.039
2.186PheGlu: 2.186 ± 0.037
1.461PhePhe: 1.461 ± 0.032
3.35PheGly: 3.35 ± 0.056
0.706PheHis: 0.706 ± 0.02
1.607PheIle: 1.607 ± 0.034
1.399PheLys: 1.399 ± 0.033
3.097PheLeu: 3.097 ± 0.05
0.902PheMet: 0.902 ± 0.024
1.196PheAsn: 1.196 ± 0.029
1.616PhePro: 1.616 ± 0.032
0.979PheGln: 0.979 ± 0.026
1.826PheArg: 1.826 ± 0.037
2.469PheSer: 2.469 ± 0.044
1.983PheThr: 1.983 ± 0.039
2.734PheVal: 2.734 ± 0.047
0.548PheTrp: 0.548 ± 0.02
0.899PheTyr: 0.899 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
9.644GlyAla: 9.644 ± 0.111
0.882GlyCys: 0.882 ± 0.026
3.862GlyAsp: 3.862 ± 0.068
4.456GlyGlu: 4.456 ± 0.05
3.419GlyPhe: 3.419 ± 0.052
7.414GlyGly: 7.414 ± 0.123
1.833GlyHis: 1.833 ± 0.038
4.118GlyIle: 4.118 ± 0.062
4.046GlyLys: 4.046 ± 0.058
9.079GlyLeu: 9.079 ± 0.096
2.389GlyMet: 2.389 ± 0.042
2.65GlyAsn: 2.65 ± 0.08
3.141GlyPro: 3.141 ± 0.046
3.481GlyGln: 3.481 ± 0.047
5.116GlyArg: 5.116 ± 0.06
4.971GlySer: 4.971 ± 0.078
4.672GlyThr: 4.672 ± 0.098
6.537GlyVal: 6.537 ± 0.076
1.432GlyTrp: 1.432 ± 0.032
2.343GlyTyr: 2.343 ± 0.039
0.0GlyXaa: 0.0 ± 0.0
His
2.606HisAla: 2.606 ± 0.046
0.248HisCys: 0.248 ± 0.012
1.044HisAsp: 1.044 ± 0.027
1.126HisGlu: 1.126 ± 0.029
0.857HisPhe: 0.857 ± 0.026
1.948HisGly: 1.948 ± 0.043
0.55HisHis: 0.55 ± 0.021
0.984HisIle: 0.984 ± 0.03
0.677HisLys: 0.677 ± 0.022
2.042HisLeu: 2.042 ± 0.045
0.483HisMet: 0.483 ± 0.019
0.532HisAsn: 0.532 ± 0.02
1.372HisPro: 1.372 ± 0.03
0.695HisGln: 0.695 ± 0.022
1.22HisArg: 1.22 ± 0.032
1.048HisSer: 1.048 ± 0.027
1.022HisThr: 1.022 ± 0.027
1.457HisVal: 1.457 ± 0.033
0.356HisTrp: 0.356 ± 0.016
0.623HisTyr: 0.623 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.022IleAla: 6.022 ± 0.066
0.444IleCys: 0.444 ± 0.017
2.71IleAsp: 2.71 ± 0.046
2.826IleGlu: 2.826 ± 0.054
1.366IlePhe: 1.366 ± 0.032
3.765IleGly: 3.765 ± 0.059
0.884IleHis: 0.884 ± 0.024
1.607IleIle: 1.607 ± 0.037
1.751IleLys: 1.751 ± 0.039
3.343IleLeu: 3.343 ± 0.046
0.825IleMet: 0.825 ± 0.028
1.545IleAsn: 1.545 ± 0.038
2.166IlePro: 2.166 ± 0.036
1.318IleGln: 1.318 ± 0.031
2.498IleArg: 2.498 ± 0.038
2.67IleSer: 2.67 ± 0.052
2.673IleThr: 2.673 ± 0.062
3.32IleVal: 3.32 ± 0.053
0.449IleTrp: 0.449 ± 0.019
1.033IleTyr: 1.033 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
5.073LysAla: 5.073 ± 0.066
0.181LysCys: 0.181 ± 0.009
1.911LysAsp: 1.911 ± 0.04
1.742LysGlu: 1.742 ± 0.045
1.147LysPhe: 1.147 ± 0.029
2.823LysGly: 2.823 ± 0.051
0.734LysHis: 0.734 ± 0.023
1.724LysIle: 1.724 ± 0.041
1.76LysLys: 1.76 ± 0.046
4.178LysLeu: 4.178 ± 0.056
0.957LysMet: 0.957 ± 0.027
1.207LysAsn: 1.207 ± 0.029
2.611LysPro: 2.611 ± 0.057
1.331LysGln: 1.331 ± 0.031
2.309LysArg: 2.309 ± 0.041
2.091LysSer: 2.091 ± 0.036
2.371LysThr: 2.371 ± 0.043
2.848LysVal: 2.848 ± 0.052
0.419LysTrp: 0.419 ± 0.016
0.78LysTyr: 0.78 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
15.112LeuAla: 15.112 ± 0.123
0.974LeuCys: 0.974 ± 0.027
5.455LeuAsp: 5.455 ± 0.059
5.206LeuGlu: 5.206 ± 0.071
3.441LeuPhe: 3.441 ± 0.057
8.817LeuGly: 8.817 ± 0.095
2.156LeuHis: 2.156 ± 0.038
4.158LeuIle: 4.158 ± 0.048
4.383LeuLys: 4.383 ± 0.066
11.597LeuLeu: 11.597 ± 0.131
2.602LeuMet: 2.602 ± 0.04
2.974LeuAsn: 2.974 ± 0.047
6.092LeuPro: 6.092 ± 0.065
4.424LeuGln: 4.424 ± 0.061
7.104LeuArg: 7.104 ± 0.08
6.296LeuSer: 6.296 ± 0.066
5.201LeuThr: 5.201 ± 0.064
7.936LeuVal: 7.936 ± 0.101
1.392LeuTrp: 1.392 ± 0.04
2.042LeuTyr: 2.042 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
3.355MetAla: 3.355 ± 0.045
0.173MetCys: 0.173 ± 0.01
1.207MetAsp: 1.207 ± 0.029
1.133MetGlu: 1.133 ± 0.028
0.793MetPhe: 0.793 ± 0.025
2.018MetGly: 2.018 ± 0.035
0.519MetHis: 0.519 ± 0.018
0.937MetIle: 0.937 ± 0.027
1.208MetLys: 1.208 ± 0.03
2.792MetLeu: 2.792 ± 0.044
0.619MetMet: 0.619 ± 0.019
0.895MetAsn: 0.895 ± 0.026
1.566MetPro: 1.566 ± 0.039
1.08MetGln: 1.08 ± 0.026
1.629MetArg: 1.629 ± 0.034
1.581MetSer: 1.581 ± 0.032
1.529MetThr: 1.529 ± 0.027
1.833MetVal: 1.833 ± 0.038
0.208MetTrp: 0.208 ± 0.011
0.426MetTyr: 0.426 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
3.475AsnAla: 3.475 ± 0.072
0.272AsnCys: 0.272 ± 0.012
1.269AsnAsp: 1.269 ± 0.036
1.219AsnGlu: 1.219 ± 0.029
1.046AsnPhe: 1.046 ± 0.027
2.377AsnGly: 2.377 ± 0.055
0.514AsnHis: 0.514 ± 0.018
1.399AsnIle: 1.399 ± 0.04
1.044AsnLys: 1.044 ± 0.037
2.749AsnLeu: 2.749 ± 0.04
0.616AsnMet: 0.616 ± 0.021
0.878AsnAsn: 0.878 ± 0.035
1.977AsnPro: 1.977 ± 0.031
0.948AsnGln: 0.948 ± 0.026
1.651AsnArg: 1.651 ± 0.035
1.392AsnSer: 1.392 ± 0.039
1.711AsnThr: 1.711 ± 0.042
2.006AsnVal: 2.006 ± 0.043
0.439AsnTrp: 0.439 ± 0.017
0.797AsnTyr: 0.797 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
7.723ProAla: 7.723 ± 0.086
0.344ProCys: 0.344 ± 0.017
2.909ProAsp: 2.909 ± 0.049
3.278ProGlu: 3.278 ± 0.055
1.804ProPhe: 1.804 ± 0.042
4.921ProGly: 4.921 ± 0.064
1.072ProHis: 1.072 ± 0.025
1.742ProIle: 1.742 ± 0.036
1.715ProLys: 1.715 ± 0.04
5.361ProLeu: 5.361 ± 0.078
1.331ProMet: 1.331 ± 0.025
1.281ProAsn: 1.281 ± 0.033
2.735ProPro: 2.735 ± 0.06
2.24ProGln: 2.24 ± 0.034
2.778ProArg: 2.778 ± 0.053
2.779ProSer: 2.779 ± 0.053
2.431ProThr: 2.431 ± 0.043
4.442ProVal: 4.442 ± 0.099
0.757ProTrp: 0.757 ± 0.022
1.211ProTyr: 1.211 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
5.937GlnAla: 5.937 ± 0.076
0.282GlnCys: 0.282 ± 0.016
1.665GlnAsp: 1.665 ± 0.036
1.593GlnGlu: 1.593 ± 0.031
1.282GlnPhe: 1.282 ± 0.028
3.227GlnGly: 3.227 ± 0.042
0.831GlnHis: 0.831 ± 0.026
1.663GlnIle: 1.663 ± 0.033
1.366GlnLys: 1.366 ± 0.029
4.302GlnLeu: 4.302 ± 0.067
0.953GlnMet: 0.953 ± 0.026
0.905GlnAsn: 0.905 ± 0.024
2.252GlnPro: 2.252 ± 0.045
1.781GlnGln: 1.781 ± 0.038
2.91GlnArg: 2.91 ± 0.047
2.066GlnSer: 2.066 ± 0.036
1.953GlnThr: 1.953 ± 0.039
2.949GlnVal: 2.949 ± 0.047
0.63GlnTrp: 0.63 ± 0.02
0.763GlnTyr: 0.763 ± 0.023
0.0GlnXaa: 0.0 ± 0.0
Arg
7.066ArgAla: 7.066 ± 0.082
0.532ArgCys: 0.532 ± 0.02
3.081ArgAsp: 3.081 ± 0.052
3.998ArgGlu: 3.998 ± 0.058
2.596ArgPhe: 2.596 ± 0.04
4.387ArgGly: 4.387 ± 0.06
1.632ArgHis: 1.632 ± 0.033
3.312ArgIle: 3.312 ± 0.048
2.303ArgLys: 2.303 ± 0.041
7.21ArgLeu: 7.21 ± 0.086
1.756ArgMet: 1.756 ± 0.032
1.778ArgAsn: 1.778 ± 0.035
2.995ArgPro: 2.995 ± 0.052
2.665ArgGln: 2.665 ± 0.042
4.306ArgArg: 4.306 ± 0.056
3.222ArgSer: 3.222 ± 0.051
2.78ArgThr: 2.78 ± 0.047
4.568ArgVal: 4.568 ± 0.058
1.029ArgTrp: 1.029 ± 0.031
1.705ArgTyr: 1.705 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.767SerAla: 6.767 ± 0.07
0.508SerCys: 0.508 ± 0.017
2.535SerAsp: 2.535 ± 0.052
2.526SerGlu: 2.526 ± 0.055
2.245SerPhe: 2.245 ± 0.04
5.592SerGly: 5.592 ± 0.1
1.137SerHis: 1.137 ± 0.027
2.418SerIle: 2.418 ± 0.048
1.811SerLys: 1.811 ± 0.039
6.077SerLeu: 6.077 ± 0.074
1.438SerMet: 1.438 ± 0.033
1.495SerAsn: 1.495 ± 0.037
3.161SerPro: 3.161 ± 0.045
1.941SerGln: 1.941 ± 0.04
3.591SerArg: 3.591 ± 0.049
3.386SerSer: 3.386 ± 0.063
3.024SerThr: 3.024 ± 0.05
3.957SerVal: 3.957 ± 0.052
0.812SerTrp: 0.812 ± 0.025
1.459SerTyr: 1.459 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
6.288ThrAla: 6.288 ± 0.084
0.36ThrCys: 0.36 ± 0.017
2.369ThrAsp: 2.369 ± 0.043
2.368ThrGlu: 2.368 ± 0.037
1.704ThrPhe: 1.704 ± 0.035
5.086ThrGly: 5.086 ± 0.104
1.147ThrHis: 1.147 ± 0.028
2.049ThrIle: 2.049 ± 0.044
1.333ThrLys: 1.333 ± 0.032
6.34ThrLeu: 6.34 ± 0.085
1.058ThrMet: 1.058 ± 0.028
1.253ThrAsn: 1.253 ± 0.039
3.62ThrPro: 3.62 ± 0.05
1.975ThrGln: 1.975 ± 0.045
3.179ThrArg: 3.179 ± 0.047
2.828ThrSer: 2.828 ± 0.052
2.859ThrThr: 2.859 ± 0.071
4.442ThrVal: 4.442 ± 0.08
0.637ThrTrp: 0.637 ± 0.022
1.089ThrTyr: 1.089 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
9.291ValAla: 9.291 ± 0.077
0.711ValCys: 0.711 ± 0.021
3.918ValAsp: 3.918 ± 0.104
3.936ValGlu: 3.936 ± 0.081
2.894ValPhe: 2.894 ± 0.048
5.537ValGly: 5.537 ± 0.063
1.515ValHis: 1.515 ± 0.032
3.396ValIle: 3.396 ± 0.046
2.928ValLys: 2.928 ± 0.04
8.376ValLeu: 8.376 ± 0.093
2.112ValMet: 2.112 ± 0.043
2.274ValAsn: 2.274 ± 0.049
3.939ValPro: 3.939 ± 0.059
2.852ValGln: 2.852 ± 0.046
4.616ValArg: 4.616 ± 0.063
4.372ValSer: 4.372 ± 0.059
4.194ValThr: 4.194 ± 0.084
6.309ValVal: 6.309 ± 0.076
0.959ValTrp: 0.959 ± 0.025
1.63ValTyr: 1.63 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.422TrpAla: 1.422 ± 0.033
0.166TrpCys: 0.166 ± 0.01
0.599TrpAsp: 0.599 ± 0.02
0.579TrpGlu: 0.579 ± 0.021
0.554TrpPhe: 0.554 ± 0.022
0.976TrpGly: 0.976 ± 0.026
0.338TrpHis: 0.338 ± 0.014
0.616TrpIle: 0.616 ± 0.023
0.536TrpLys: 0.536 ± 0.019
2.051TrpLeu: 2.051 ± 0.044
0.457TrpMet: 0.457 ± 0.016
0.41TrpAsn: 0.41 ± 0.019
0.722TrpPro: 0.722 ± 0.023
0.702TrpGln: 0.702 ± 0.02
1.125TrpArg: 1.125 ± 0.032
0.775TrpSer: 0.775 ± 0.026
0.724TrpThr: 0.724 ± 0.022
1.008TrpVal: 1.008 ± 0.031
0.257TrpTrp: 0.257 ± 0.013
0.334TrpTyr: 0.334 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.66TyrAla: 2.66 ± 0.042
0.258TyrCys: 0.258 ± 0.016
1.201TyrAsp: 1.201 ± 0.042
1.215TyrGlu: 1.215 ± 0.031
0.962TyrPhe: 0.962 ± 0.026
2.152TyrGly: 2.152 ± 0.042
0.413TyrHis: 0.413 ± 0.017
0.829TyrIle: 0.829 ± 0.021
0.856TyrLys: 0.856 ± 0.026
2.415TyrLeu: 2.415 ± 0.041
0.473TyrMet: 0.473 ± 0.019
0.66TyrAsn: 0.66 ± 0.025
1.203TyrPro: 1.203 ± 0.027
0.855TyrGln: 0.855 ± 0.024
1.607TyrArg: 1.607 ± 0.033
1.316TyrSer: 1.316 ± 0.029
1.318TyrThr: 1.318 ± 0.037
1.662TyrVal: 1.662 ± 0.03
0.378TyrTrp: 0.378 ± 0.018
0.595TyrTyr: 0.595 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4751 proteins (1515684 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski