Halomonas pacifica

Taxonomy: cellular organisms; Bacteria; Proteobacteria;

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3562 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A510XCT0|A0A510XCT0_9GAMM Transcriptional regulator OS=Halomonas pacifica OX=77098 GN=HPA02_06130 PE=4 SV=1
MM1 pKa = 7.71KK2 pKa = 9.06KK3 pKa = 8.27TLLATAIAGAMGLSAASAQAATVYY27 pKa = 10.67NQDD30 pKa = 3.01GTKK33 pKa = 10.43LDD35 pKa = 3.38IYY37 pKa = 11.46GNIQLAYY44 pKa = 9.51YY45 pKa = 10.5SVDD48 pKa = 3.03AGNNSVDD55 pKa = 3.55EE56 pKa = 4.38LADD59 pKa = 3.67NGSTFGFAAEE69 pKa = 4.25HH70 pKa = 6.26VINPGLTGYY79 pKa = 10.89LKK81 pKa = 11.21LEE83 pKa = 4.31FDD85 pKa = 4.44DD86 pKa = 6.14FKK88 pKa = 11.65ADD90 pKa = 4.11EE91 pKa = 4.59IKK93 pKa = 9.93TAGRR97 pKa = 11.84DD98 pKa = 3.5SGDD101 pKa = 3.12QAYY104 pKa = 10.71VGLRR108 pKa = 11.84GDD110 pKa = 3.76FGDD113 pKa = 4.55ARR115 pKa = 11.84LGSYY119 pKa = 10.07DD120 pKa = 4.35ALIDD124 pKa = 4.71DD125 pKa = 5.85WIQDD129 pKa = 4.02PITNNEE135 pKa = 3.83FFDD138 pKa = 4.31VNDD141 pKa = 3.68SSSQRR146 pKa = 11.84GVGGINEE153 pKa = 4.34TDD155 pKa = 2.97KK156 pKa = 10.67FTYY159 pKa = 9.41TSPSFGGFQFALGTQYY175 pKa = 10.91KK176 pKa = 10.56GEE178 pKa = 4.25AEE180 pKa = 4.28SEE182 pKa = 4.26NVTDD186 pKa = 4.18SGNASFFGGARR197 pKa = 11.84YY198 pKa = 7.43TVGAFSVAAVYY209 pKa = 10.97DD210 pKa = 3.78NLDD213 pKa = 3.65NYY215 pKa = 11.21ADD217 pKa = 4.25AYY219 pKa = 10.18QNNGQFGDD227 pKa = 3.77QYY229 pKa = 11.64GLTAQYY235 pKa = 10.42TIDD238 pKa = 3.9SLRR241 pKa = 11.84LAAKK245 pKa = 10.13VEE247 pKa = 4.17HH248 pKa = 6.96FDD250 pKa = 4.15GDD252 pKa = 3.98MANTDD257 pKa = 3.34ADD259 pKa = 4.61LYY261 pKa = 11.13ALSARR266 pKa = 11.84YY267 pKa = 9.47GYY269 pKa = 11.06GVGDD273 pKa = 3.27VYY275 pKa = 11.24GAYY278 pKa = 9.96QRR280 pKa = 11.84VDD282 pKa = 3.71DD283 pKa = 5.38DD284 pKa = 4.95LEE286 pKa = 6.03ADD288 pKa = 3.53EE289 pKa = 5.62RR290 pKa = 11.84DD291 pKa = 3.75EE292 pKa = 4.43FVVGATYY299 pKa = 10.98NLSDD303 pKa = 5.42AMYY306 pKa = 8.57TWAEE310 pKa = 3.89AAWYY314 pKa = 10.0DD315 pKa = 3.57NAQDD319 pKa = 3.52VGDD322 pKa = 4.14GVAVGVTYY330 pKa = 10.66LFF332 pKa = 4.38

Molecular weight: 35.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A510X987|A0A510X987_9GAMM Outer membrane protein OS=Halomonas pacifica OX=77098 GN=HPA02_22460 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3562

0

3562

1139457

40

3557

319.9

34.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.334 ± 0.053

0.949 ± 0.014

5.436 ± 0.037

6.801 ± 0.046

3.334 ± 0.027

8.616 ± 0.041

2.37 ± 0.021

4.289 ± 0.031

2.267 ± 0.032

12.239 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.279 ± 0.022

2.158 ± 0.023

5.178 ± 0.03

3.646 ± 0.029

7.87 ± 0.046

4.905 ± 0.023

4.606 ± 0.026

6.916 ± 0.038

1.504 ± 0.018

2.303 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski