Mycobacterium phage Minnie

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WPZ7|A0A5Q2WPZ7_9CAUD Uncharacterized protein OS=Mycobacterium phage Minnie OX=2653764 GN=80 PE=4 SV=1
MM1 pKa = 7.36VSPASPLPAQRR12 pKa = 11.84VEE14 pKa = 4.21VTAVTGICICTHH26 pKa = 5.57YY27 pKa = 9.79RR28 pKa = 11.84TEE30 pKa = 4.16HH31 pKa = 6.94DD32 pKa = 3.22EE33 pKa = 4.48HH34 pKa = 7.05GNCLASDD41 pKa = 4.55LFGEE45 pKa = 4.76PCDD48 pKa = 4.17CPGYY52 pKa = 10.79EE53 pKa = 4.38PDD55 pKa = 4.48EE56 pKa = 4.82EE57 pKa = 4.78EE58 pKa = 5.62DD59 pKa = 4.83DD60 pKa = 3.96EE61 pKa = 4.68TT62 pKa = 5.08

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WM48|A0A5Q2WM48_9CAUD Tail terminator OS=Mycobacterium phage Minnie OX=2653764 GN=10 PE=4 SV=1
MM1 pKa = 7.2TAVCEE6 pKa = 4.13QCGSDD11 pKa = 3.46FVRR14 pKa = 11.84PARR17 pKa = 11.84RR18 pKa = 11.84GRR20 pKa = 11.84PRR22 pKa = 11.84LTCSDD27 pKa = 3.43YY28 pKa = 11.05CKK30 pKa = 10.0RR31 pKa = 11.84ARR33 pKa = 11.84RR34 pKa = 11.84SRR36 pKa = 11.84LQLDD40 pKa = 2.98RR41 pKa = 11.84DD42 pKa = 3.14KK43 pKa = 10.95RR44 pKa = 11.84YY45 pKa = 10.28RR46 pKa = 11.84EE47 pKa = 3.71EE48 pKa = 4.6HH49 pKa = 5.43GAWRR53 pKa = 11.84EE54 pKa = 3.73KK55 pKa = 10.66RR56 pKa = 11.84RR57 pKa = 11.84MRR59 pKa = 11.84SYY61 pKa = 11.32PEE63 pKa = 3.73TCGVCGRR70 pKa = 11.84TFDD73 pKa = 4.93AKK75 pKa = 10.68RR76 pKa = 11.84KK77 pKa = 8.62GQALCSVEE85 pKa = 4.0CQFAVRR91 pKa = 11.84NRR93 pKa = 11.84AAHH96 pKa = 5.31EE97 pKa = 4.21ANRR100 pKa = 11.84QSSIQRR106 pKa = 11.84RR107 pKa = 11.84LPVLYY112 pKa = 9.92TGDD115 pKa = 3.4GVVRR119 pKa = 11.84ATHH122 pKa = 5.49RR123 pKa = 11.84TWTSGLCASCGEE135 pKa = 4.21WFVAGARR142 pKa = 11.84ARR144 pKa = 11.84YY145 pKa = 8.96CSNRR149 pKa = 11.84CAQRR153 pKa = 11.84AHH155 pKa = 5.59SEE157 pKa = 3.71RR158 pKa = 11.84RR159 pKa = 11.84RR160 pKa = 11.84ARR162 pKa = 11.84EE163 pKa = 3.63AGATTSSRR171 pKa = 11.84VRR173 pKa = 11.84RR174 pKa = 11.84RR175 pKa = 11.84EE176 pKa = 3.36IFEE179 pKa = 4.08RR180 pKa = 11.84DD181 pKa = 3.43GYY183 pKa = 10.65RR184 pKa = 11.84CHH186 pKa = 7.51LCGEE190 pKa = 4.52MTDD193 pKa = 3.86PTQQVPHH200 pKa = 6.77PRR202 pKa = 11.84APTIDD207 pKa = 3.39QRR209 pKa = 11.84PAIEE213 pKa = 4.34SRR215 pKa = 11.84WVARR219 pKa = 11.84ARR221 pKa = 11.84EE222 pKa = 4.14LPVRR226 pKa = 11.84VLPVQLGQEE235 pKa = 4.42RR236 pKa = 11.84PCGLVTPANGEE247 pKa = 4.07LAA249 pKa = 3.76

Molecular weight: 28.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

100

0

100

18955

30

1213

189.6

20.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.382 ± 0.448

1.235 ± 0.146

6.552 ± 0.215

5.872 ± 0.287

3.034 ± 0.216

8.979 ± 0.555

2.348 ± 0.199

4.131 ± 0.181

3.419 ± 0.214

7.391 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.184 ± 0.116

3.371 ± 0.163

5.909 ± 0.197

3.498 ± 0.154

7.154 ± 0.417

5.845 ± 0.203

6.494 ± 0.247

7.386 ± 0.236

2.269 ± 0.113

2.548 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski