Amino acid dipepetide frequency for Mycobacterium phage Minnie

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.192AlaAla: 14.192 ± 1.395
0.897AlaCys: 0.897 ± 0.22
6.964AlaAsp: 6.964 ± 0.641
6.384AlaGlu: 6.384 ± 0.624
2.902AlaPhe: 2.902 ± 0.443
9.919AlaGly: 9.919 ± 1.189
2.691AlaHis: 2.691 ± 0.362
4.221AlaIle: 4.221 ± 0.474
4.379AlaLys: 4.379 ± 0.554
7.386AlaLeu: 7.386 ± 0.561
2.321AlaMet: 2.321 ± 0.39
3.06AlaAsn: 3.06 ± 0.439
5.012AlaPro: 5.012 ± 0.565
3.113AlaGln: 3.113 ± 0.373
7.228AlaArg: 7.228 ± 0.672
5.434AlaSer: 5.434 ± 0.654
6.067AlaThr: 6.067 ± 0.527
7.07AlaVal: 7.07 ± 0.518
2.427AlaTrp: 2.427 ± 0.356
2.163AlaTyr: 2.163 ± 0.267
0.0AlaXaa: 0.0 ± 0.0
Cys
0.739CysAla: 0.739 ± 0.234
0.0CysCys: 0.0 ± 0.0
1.425CysAsp: 1.425 ± 0.346
0.791CysGlu: 0.791 ± 0.196
0.264CysPhe: 0.264 ± 0.116
1.741CysGly: 1.741 ± 0.476
0.317CysHis: 0.317 ± 0.113
0.264CysIle: 0.264 ± 0.119
0.686CysLys: 0.686 ± 0.185
0.633CysLeu: 0.633 ± 0.214
0.264CysMet: 0.264 ± 0.124
0.264CysAsn: 0.264 ± 0.112
1.266CysPro: 1.266 ± 0.276
0.317CysGln: 0.317 ± 0.122
0.58CysArg: 0.58 ± 0.176
0.791CysSer: 0.791 ± 0.27
0.58CysThr: 0.58 ± 0.183
0.739CysVal: 0.739 ± 0.176
0.422CysTrp: 0.422 ± 0.135
0.264CysTyr: 0.264 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
6.753AspAla: 6.753 ± 0.625
0.844AspCys: 0.844 ± 0.218
4.326AspAsp: 4.326 ± 0.538
3.588AspGlu: 3.588 ± 0.499
1.794AspPhe: 1.794 ± 0.256
6.595AspGly: 6.595 ± 0.602
1.425AspHis: 1.425 ± 0.301
2.269AspIle: 2.269 ± 0.341
1.794AspLys: 1.794 ± 0.26
5.487AspLeu: 5.487 ± 0.557
1.055AspMet: 1.055 ± 0.272
1.636AspAsn: 1.636 ± 0.394
4.959AspPro: 4.959 ± 0.538
2.321AspGln: 2.321 ± 0.297
4.854AspArg: 4.854 ± 0.51
3.482AspSer: 3.482 ± 0.5
3.957AspThr: 3.957 ± 0.533
4.485AspVal: 4.485 ± 0.537
1.741AspTrp: 1.741 ± 0.3
2.163AspTyr: 2.163 ± 0.313
0.0AspXaa: 0.0 ± 0.0
Glu
5.751GluAla: 5.751 ± 0.682
0.791GluCys: 0.791 ± 0.203
3.377GluAsp: 3.377 ± 0.374
2.532GluGlu: 2.532 ± 0.428
2.216GluPhe: 2.216 ± 0.412
3.166GluGly: 3.166 ± 0.35
1.53GluHis: 1.53 ± 0.379
2.532GluIle: 2.532 ± 0.367
2.216GluLys: 2.216 ± 0.353
6.542GluLeu: 6.542 ± 0.682
1.583GluMet: 1.583 ± 0.291
2.163GluAsn: 2.163 ± 0.287
2.427GluPro: 2.427 ± 0.414
2.955GluGln: 2.955 ± 0.344
4.907GluArg: 4.907 ± 0.512
3.113GluSer: 3.113 ± 0.426
4.274GluThr: 4.274 ± 0.467
3.535GluVal: 3.535 ± 0.348
1.213GluTrp: 1.213 ± 0.231
1.899GluTyr: 1.899 ± 0.29
0.0GluXaa: 0.0 ± 0.0
Phe
3.324PheAla: 3.324 ± 0.369
0.264PheCys: 0.264 ± 0.101
2.48PheAsp: 2.48 ± 0.487
1.794PheGlu: 1.794 ± 0.334
0.739PhePhe: 0.739 ± 0.266
3.166PheGly: 3.166 ± 0.579
0.528PheHis: 0.528 ± 0.22
1.002PheIle: 1.002 ± 0.295
0.95PheLys: 0.95 ± 0.223
1.899PheLeu: 1.899 ± 0.269
0.739PheMet: 0.739 ± 0.227
1.319PheAsn: 1.319 ± 0.287
1.583PhePro: 1.583 ± 0.298
1.055PheGln: 1.055 ± 0.259
1.477PheArg: 1.477 ± 0.221
1.477PheSer: 1.477 ± 0.252
2.48PheThr: 2.48 ± 0.349
2.374PheVal: 2.374 ± 0.273
0.844PheTrp: 0.844 ± 0.219
0.844PheTyr: 0.844 ± 0.271
0.0PheXaa: 0.0 ± 0.0
Gly
9.338GlyAla: 9.338 ± 1.101
1.161GlyCys: 1.161 ± 0.287
5.698GlyAsp: 5.698 ± 0.54
5.329GlyGlu: 5.329 ± 0.586
2.902GlyPhe: 2.902 ± 0.467
10.763GlyGly: 10.763 ± 2.355
2.11GlyHis: 2.11 ± 0.415
4.168GlyIle: 4.168 ± 0.512
2.48GlyLys: 2.48 ± 0.302
6.489GlyLeu: 6.489 ± 0.61
2.11GlyMet: 2.11 ± 0.41
2.743GlyAsn: 2.743 ± 0.337
3.851GlyPro: 3.851 ± 0.613
2.216GlyGln: 2.216 ± 0.439
6.067GlyArg: 6.067 ± 0.661
6.12GlySer: 6.12 ± 0.816
6.015GlyThr: 6.015 ± 0.69
5.698GlyVal: 5.698 ± 0.494
2.058GlyTrp: 2.058 ± 0.294
2.48GlyTyr: 2.48 ± 0.393
0.0GlyXaa: 0.0 ± 0.0
His
1.794HisAla: 1.794 ± 0.346
0.211HisCys: 0.211 ± 0.121
1.213HisAsp: 1.213 ± 0.249
1.372HisGlu: 1.372 ± 0.334
0.475HisPhe: 0.475 ± 0.136
2.058HisGly: 2.058 ± 0.293
1.002HisHis: 1.002 ± 0.251
1.583HisIle: 1.583 ± 0.3
1.055HisLys: 1.055 ± 0.287
1.53HisLeu: 1.53 ± 0.303
0.58HisMet: 0.58 ± 0.15
0.844HisAsn: 0.844 ± 0.197
1.477HisPro: 1.477 ± 0.28
0.791HisGln: 0.791 ± 0.193
2.163HisArg: 2.163 ± 0.425
1.002HisSer: 1.002 ± 0.219
1.266HisThr: 1.266 ± 0.269
1.688HisVal: 1.688 ± 0.325
0.58HisTrp: 0.58 ± 0.181
0.791HisTyr: 0.791 ± 0.191
0.0HisXaa: 0.0 ± 0.0
Ile
4.801IleAla: 4.801 ± 0.478
0.58IleCys: 0.58 ± 0.198
3.64IleAsp: 3.64 ± 0.471
3.693IleGlu: 3.693 ± 0.406
0.791IlePhe: 0.791 ± 0.209
3.588IleGly: 3.588 ± 0.502
1.425IleHis: 1.425 ± 0.297
1.213IleIle: 1.213 ± 0.252
1.002IleLys: 1.002 ± 0.245
2.163IleLeu: 2.163 ± 0.369
0.317IleMet: 0.317 ± 0.123
1.213IleAsn: 1.213 ± 0.254
3.113IlePro: 3.113 ± 0.414
1.583IleGln: 1.583 ± 0.278
2.163IleArg: 2.163 ± 0.329
2.058IleSer: 2.058 ± 0.338
3.377IleThr: 3.377 ± 0.43
3.113IleVal: 3.113 ± 0.409
0.791IleTrp: 0.791 ± 0.185
0.686IleTyr: 0.686 ± 0.204
0.0IleXaa: 0.0 ± 0.0
Lys
4.01LysAla: 4.01 ± 0.512
0.475LysCys: 0.475 ± 0.163
1.636LysAsp: 1.636 ± 0.256
1.636LysGlu: 1.636 ± 0.301
1.266LysPhe: 1.266 ± 0.211
2.532LysGly: 2.532 ± 0.338
1.055LysHis: 1.055 ± 0.238
0.686LysIle: 0.686 ± 0.246
1.425LysLys: 1.425 ± 0.385
2.269LysLeu: 2.269 ± 0.367
0.528LysMet: 0.528 ± 0.184
1.266LysAsn: 1.266 ± 0.249
2.427LysPro: 2.427 ± 0.391
1.636LysGln: 1.636 ± 0.306
2.48LysArg: 2.48 ± 0.402
2.216LysSer: 2.216 ± 0.344
1.952LysThr: 1.952 ± 0.294
2.849LysVal: 2.849 ± 0.38
0.739LysTrp: 0.739 ± 0.18
1.055LysTyr: 1.055 ± 0.278
0.0LysXaa: 0.0 ± 0.0
Leu
7.65LeuAla: 7.65 ± 0.639
1.055LeuCys: 1.055 ± 0.271
4.062LeuAsp: 4.062 ± 0.453
4.748LeuGlu: 4.748 ± 0.439
2.691LeuPhe: 2.691 ± 0.319
5.54LeuGly: 5.54 ± 0.503
0.739LeuHis: 0.739 ± 0.167
3.007LeuIle: 3.007 ± 0.406
2.163LeuLys: 2.163 ± 0.326
4.696LeuLeu: 4.696 ± 0.476
1.794LeuMet: 1.794 ± 0.309
2.902LeuAsn: 2.902 ± 0.424
5.329LeuPro: 5.329 ± 0.637
2.849LeuGln: 2.849 ± 0.356
5.17LeuArg: 5.17 ± 0.603
5.54LeuSer: 5.54 ± 0.522
5.434LeuThr: 5.434 ± 0.616
4.959LeuVal: 4.959 ± 0.578
1.583LeuTrp: 1.583 ± 0.306
2.005LeuTyr: 2.005 ± 0.363
0.0LeuXaa: 0.0 ± 0.0
Met
2.005MetAla: 2.005 ± 0.275
0.317MetCys: 0.317 ± 0.161
1.53MetAsp: 1.53 ± 0.29
1.002MetGlu: 1.002 ± 0.189
0.58MetPhe: 0.58 ± 0.171
1.636MetGly: 1.636 ± 0.249
0.211MetHis: 0.211 ± 0.102
0.897MetIle: 0.897 ± 0.227
0.844MetLys: 0.844 ± 0.213
1.636MetLeu: 1.636 ± 0.251
0.58MetMet: 0.58 ± 0.193
1.055MetAsn: 1.055 ± 0.2
1.108MetPro: 1.108 ± 0.248
0.475MetGln: 0.475 ± 0.118
1.688MetArg: 1.688 ± 0.294
2.427MetSer: 2.427 ± 0.402
2.11MetThr: 2.11 ± 0.258
1.266MetVal: 1.266 ± 0.306
0.264MetTrp: 0.264 ± 0.113
0.211MetTyr: 0.211 ± 0.102
0.0MetXaa: 0.0 ± 0.0
Asn
3.113AsnAla: 3.113 ± 0.404
0.475AsnCys: 0.475 ± 0.157
1.847AsnAsp: 1.847 ± 0.29
1.53AsnGlu: 1.53 ± 0.323
0.95AsnPhe: 0.95 ± 0.294
3.746AsnGly: 3.746 ± 0.525
1.002AsnHis: 1.002 ± 0.243
1.425AsnIle: 1.425 ± 0.412
0.95AsnLys: 0.95 ± 0.26
2.638AsnLeu: 2.638 ± 0.368
0.58AsnMet: 0.58 ± 0.151
1.899AsnAsn: 1.899 ± 0.342
2.902AsnPro: 2.902 ± 0.338
1.161AsnGln: 1.161 ± 0.262
2.849AsnArg: 2.849 ± 0.39
1.213AsnSer: 1.213 ± 0.29
2.058AsnThr: 2.058 ± 0.287
2.058AsnVal: 2.058 ± 0.301
0.528AsnTrp: 0.528 ± 0.132
0.791AsnTyr: 0.791 ± 0.176
0.0AsnXaa: 0.0 ± 0.0
Pro
5.434ProAla: 5.434 ± 0.587
0.844ProCys: 0.844 ± 0.242
4.379ProAsp: 4.379 ± 0.479
4.274ProGlu: 4.274 ± 0.401
1.636ProPhe: 1.636 ± 0.25
6.331ProGly: 6.331 ± 0.579
1.847ProHis: 1.847 ± 0.306
1.741ProIle: 1.741 ± 0.269
2.321ProLys: 2.321 ± 0.409
4.115ProLeu: 4.115 ± 0.48
1.372ProMet: 1.372 ± 0.327
1.952ProAsn: 1.952 ± 0.287
3.377ProPro: 3.377 ± 0.578
2.321ProGln: 2.321 ± 0.356
3.482ProArg: 3.482 ± 0.475
2.532ProSer: 2.532 ± 0.336
3.482ProThr: 3.482 ± 0.428
5.012ProVal: 5.012 ± 0.473
1.161ProTrp: 1.161 ± 0.284
1.477ProTyr: 1.477 ± 0.277
0.0ProXaa: 0.0 ± 0.0
Gln
4.062GlnAla: 4.062 ± 0.422
0.317GlnCys: 0.317 ± 0.12
1.583GlnAsp: 1.583 ± 0.236
1.583GlnGlu: 1.583 ± 0.266
0.844GlnPhe: 0.844 ± 0.22
2.374GlnGly: 2.374 ± 0.389
0.897GlnHis: 0.897 ± 0.208
1.847GlnIle: 1.847 ± 0.289
1.161GlnLys: 1.161 ± 0.217
3.271GlnLeu: 3.271 ± 0.422
0.686GlnMet: 0.686 ± 0.202
1.002GlnAsn: 1.002 ± 0.218
2.532GlnPro: 2.532 ± 0.361
1.477GlnGln: 1.477 ± 0.275
3.06GlnArg: 3.06 ± 0.467
2.427GlnSer: 2.427 ± 0.322
1.899GlnThr: 1.899 ± 0.335
2.532GlnVal: 2.532 ± 0.343
0.528GlnTrp: 0.528 ± 0.169
0.897GlnTyr: 0.897 ± 0.225
0.0GlnXaa: 0.0 ± 0.0
Arg
6.859ArgAla: 6.859 ± 0.673
1.266ArgCys: 1.266 ± 0.335
4.643ArgAsp: 4.643 ± 0.581
4.537ArgGlu: 4.537 ± 0.552
2.743ArgPhe: 2.743 ± 0.371
4.537ArgGly: 4.537 ± 0.475
1.213ArgHis: 1.213 ± 0.252
3.799ArgIle: 3.799 ± 0.523
2.585ArgLys: 2.585 ± 0.347
5.329ArgLeu: 5.329 ± 0.587
2.11ArgMet: 2.11 ± 0.314
2.427ArgAsn: 2.427 ± 0.378
3.693ArgPro: 3.693 ± 0.447
2.163ArgGln: 2.163 ± 0.364
7.017ArgArg: 7.017 ± 0.88
3.535ArgSer: 3.535 ± 0.444
3.588ArgThr: 3.588 ± 0.409
5.276ArgVal: 5.276 ± 0.632
2.005ArgTrp: 2.005 ± 0.365
2.216ArgTyr: 2.216 ± 0.32
0.0ArgXaa: 0.0 ± 0.0
Ser
5.487SerAla: 5.487 ± 0.714
0.422SerCys: 0.422 ± 0.153
4.115SerAsp: 4.115 ± 0.564
3.007SerGlu: 3.007 ± 0.303
1.952SerPhe: 1.952 ± 0.395
6.015SerGly: 6.015 ± 0.838
1.266SerHis: 1.266 ± 0.223
2.585SerIle: 2.585 ± 0.343
2.321SerLys: 2.321 ± 0.364
4.115SerLeu: 4.115 ± 0.423
1.636SerMet: 1.636 ± 0.259
1.741SerAsn: 1.741 ± 0.294
3.218SerPro: 3.218 ± 0.312
1.847SerGln: 1.847 ± 0.316
3.799SerArg: 3.799 ± 0.463
4.01SerSer: 4.01 ± 0.507
3.113SerThr: 3.113 ± 0.428
4.907SerVal: 4.907 ± 0.525
1.425SerTrp: 1.425 ± 0.214
1.477SerTyr: 1.477 ± 0.251
0.0SerXaa: 0.0 ± 0.0
Thr
6.384ThrAla: 6.384 ± 0.59
0.528ThrCys: 0.528 ± 0.18
4.274ThrAsp: 4.274 ± 0.474
3.482ThrGlu: 3.482 ± 0.344
2.005ThrPhe: 2.005 ± 0.312
6.806ThrGly: 6.806 ± 0.676
1.794ThrHis: 1.794 ± 0.347
3.113ThrIle: 3.113 ± 0.428
2.058ThrLys: 2.058 ± 0.34
4.485ThrLeu: 4.485 ± 0.408
1.161ThrMet: 1.161 ± 0.205
2.269ThrAsn: 2.269 ± 0.397
4.221ThrPro: 4.221 ± 0.454
1.952ThrGln: 1.952 ± 0.352
3.746ThrArg: 3.746 ± 0.452
3.535ThrSer: 3.535 ± 0.375
4.643ThrThr: 4.643 ± 0.569
5.381ThrVal: 5.381 ± 0.556
1.372ThrTrp: 1.372 ± 0.22
1.741ThrTyr: 1.741 ± 0.279
0.0ThrXaa: 0.0 ± 0.0
Val
7.65ValAla: 7.65 ± 0.53
1.161ValCys: 1.161 ± 0.244
5.276ValAsp: 5.276 ± 0.516
4.643ValGlu: 4.643 ± 0.453
2.005ValPhe: 2.005 ± 0.384
5.698ValGly: 5.698 ± 0.593
1.477ValHis: 1.477 ± 0.253
3.166ValIle: 3.166 ± 0.431
2.374ValLys: 2.374 ± 0.334
5.223ValLeu: 5.223 ± 0.533
1.53ValMet: 1.53 ± 0.279
2.532ValAsn: 2.532 ± 0.342
3.904ValPro: 3.904 ± 0.326
2.796ValGln: 2.796 ± 0.34
4.696ValArg: 4.696 ± 0.609
5.012ValSer: 5.012 ± 0.636
5.276ValThr: 5.276 ± 0.49
6.542ValVal: 6.542 ± 0.695
1.53ValTrp: 1.53 ± 0.31
1.372ValTyr: 1.372 ± 0.332
0.0ValXaa: 0.0 ± 0.0
Trp
1.899TrpAla: 1.899 ± 0.272
0.317TrpCys: 0.317 ± 0.143
1.319TrpAsp: 1.319 ± 0.262
1.161TrpGlu: 1.161 ± 0.308
0.686TrpPhe: 0.686 ± 0.162
1.319TrpGly: 1.319 ± 0.232
0.633TrpHis: 0.633 ± 0.203
0.844TrpIle: 0.844 ± 0.179
0.844TrpLys: 0.844 ± 0.182
1.741TrpLeu: 1.741 ± 0.339
0.633TrpMet: 0.633 ± 0.19
0.686TrpAsn: 0.686 ± 0.198
1.002TrpPro: 1.002 ± 0.269
1.002TrpGln: 1.002 ± 0.271
2.11TrpArg: 2.11 ± 0.381
1.319TrpSer: 1.319 ± 0.33
1.636TrpThr: 1.636 ± 0.274
2.005TrpVal: 2.005 ± 0.343
1.108TrpTrp: 1.108 ± 0.236
0.422TrpTyr: 0.422 ± 0.171
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.585TyrAla: 2.585 ± 0.41
0.422TyrCys: 0.422 ± 0.171
1.741TyrAsp: 1.741 ± 0.339
1.636TyrGlu: 1.636 ± 0.232
0.897TyrPhe: 0.897 ± 0.21
2.269TyrGly: 2.269 ± 0.331
0.317TyrHis: 0.317 ± 0.12
0.95TyrIle: 0.95 ± 0.185
0.58TyrLys: 0.58 ± 0.155
2.269TyrLeu: 2.269 ± 0.428
0.211TyrMet: 0.211 ± 0.098
0.739TyrAsn: 0.739 ± 0.237
1.688TyrPro: 1.688 ± 0.218
0.95TyrGln: 0.95 ± 0.273
2.005TyrArg: 2.005 ± 0.312
1.213TyrSer: 1.213 ± 0.247
1.741TyrThr: 1.741 ± 0.305
2.374TyrVal: 2.374 ± 0.333
0.369TyrTrp: 0.369 ± 0.128
0.528TyrTyr: 0.528 ± 0.139
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 100 proteins (18955 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski