Corynebacterium pseudotuberculosis (strain C231)
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2001 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D9QB02|D9QB02_CORP2 Phosphatidylglycerophosphate synthase OS=Corynebacterium pseudotuberculosis (strain C231) OX=681645 GN=pgsA1 PE=3 SV=1
MM1 pKa = 7.12 STDD4 pKa = 3.63 LLSIGMTFDD13 pKa = 2.91 KK14 pKa = 10.66 WQDD17 pKa = 3.12 AVEE20 pKa = 3.98 AAIATDD26 pKa = 3.42 KK27 pKa = 11.17 LAVTGEE33 pKa = 4.0 VRR35 pKa = 11.84 GGQLIQYY42 pKa = 9.59 ADD44 pKa = 3.77 DD45 pKa = 4.58 SGAQINILAVEE56 pKa = 4.34 PFATFAGFDD65 pKa = 3.54 SVTQSFAHH73 pKa = 6.19 ISMVNDD79 pKa = 3.01 VLALCEE85 pKa = 3.84 IVDD88 pKa = 3.91 YY89 pKa = 11.17 NGQEE93 pKa = 4.23 VTTVACNLAQGPLLVDD109 pKa = 3.51 EE110 pKa = 5.51 PEE112 pKa = 4.32 QRR114 pKa = 11.84 WQQLGLSALAFDD126 pKa = 5.03 VITYY130 pKa = 7.26 PTIADD135 pKa = 4.31 FEE137 pKa = 4.44 QASPEE142 pKa = 3.81 ILGTVVSEE150 pKa = 4.38 GALTVMSGNGTAAPDD165 pKa = 3.26 AFATFNVRR173 pKa = 11.84 VLSAEE178 pKa = 3.79 YY179 pKa = 8.57 RR180 pKa = 11.84 TNTLTGSRR188 pKa = 11.84 FIHH191 pKa = 5.91 ATVDD195 pKa = 3.44 GPFPFDD201 pKa = 3.14 VCLPDD206 pKa = 4.3 AASLPEE212 pKa = 4.58 RR213 pKa = 11.84 DD214 pKa = 3.62 SVISGKK220 pKa = 10.35 AVLVGSVSVPEE231 pKa = 4.2 SGGCGSGGGGCGSGGCGCGGHH252 pKa = 6.68
Molecular weight: 25.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.935
IPC_protein 3.91
Toseland 3.706
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.821
Rodwell 3.745
Grimsley 3.617
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.228
Thurlkill 3.757
EMBOSS 3.834
Sillero 4.037
Patrickios 1.1
IPC_peptide 3.884
IPC2_peptide 4.012
IPC2.peptide.svr19 3.903
Protein with the highest isoelectric point:
>tr|D9QE58|D9QE58_CORP2 Thiol-disulfide isomerase/thioredoxin OS=Corynebacterium pseudotuberculosis (strain C231) OX=681645 GN=ccsX PE=4 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.76 KK15 pKa = 9.54 KK16 pKa = 9.72 HH17 pKa = 5.63 RR18 pKa = 11.84 KK19 pKa = 7.56 LLRR22 pKa = 11.84 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2001
0
2001
659651
33
3032
329.7
35.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.92 ± 0.065
0.808 ± 0.015
5.631 ± 0.048
6.069 ± 0.059
3.456 ± 0.033
8.052 ± 0.052
2.263 ± 0.028
5.733 ± 0.045
3.896 ± 0.052
9.661 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.234 ± 0.025
3.121 ± 0.032
4.84 ± 0.032
3.352 ± 0.028
5.814 ± 0.052
6.5 ± 0.042
5.965 ± 0.035
8.088 ± 0.044
1.345 ± 0.024
2.25 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here