Catenovulum agarivorans DS-2
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3835 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W7QEY9|W7QEY9_9ALTE L-rhamnose mutarotase OS=Catenovulum agarivorans DS-2 OX=1328313 GN=rhaM PE=3 SV=1
MM1 pKa = 7.36 SLSACKK7 pKa = 9.1 DD8 pKa = 3.41 TKK10 pKa = 11.14 VDD12 pKa = 3.21 IDD14 pKa = 3.98 PPEE17 pKa = 4.52 EE18 pKa = 3.98 PTPAPSPTPTPTPTPTPTPTPANTAPTVDD47 pKa = 4.0 MLDD50 pKa = 3.51 SATVEE55 pKa = 3.83 QGLSIEE61 pKa = 4.5 LNWTISDD68 pKa = 4.08 AEE70 pKa = 4.1 QDD72 pKa = 3.67 NLTCEE77 pKa = 3.91 IDD79 pKa = 4.58 AGDD82 pKa = 4.01 SSNPIAITDD91 pKa = 3.61 CAYY94 pKa = 9.76 TGKK97 pKa = 7.64 TTLTYY102 pKa = 10.31 SEE104 pKa = 4.41 SGQYY108 pKa = 9.44 QIKK111 pKa = 8.21 FTADD115 pKa = 3.15 DD116 pKa = 4.61 GEE118 pKa = 4.49 FSSEE122 pKa = 3.59 QTLNITISQPAPQFTLAASATAPAALYY149 pKa = 10.74 LRR151 pKa = 11.84 GFNDD155 pKa = 4.66 DD156 pKa = 3.85 WDD158 pKa = 4.11 TTNPFTLHH166 pKa = 6.63 DD167 pKa = 5.09 GIYY170 pKa = 10.42 VIEE173 pKa = 4.29 FDD175 pKa = 3.96 GTFAQQMVKK184 pKa = 10.18 VASEE188 pKa = 3.84 DD189 pKa = 3.24 WSAVNCGGFALSANTAPVDD208 pKa = 4.01 LTCGGNPDD216 pKa = 3.6 NGSITFEE223 pKa = 3.65 EE224 pKa = 4.47 DD225 pKa = 2.5 ALYY228 pKa = 10.49 RR229 pKa = 11.84 IGVSYY234 pKa = 10.92 QDD236 pKa = 4.42 DD237 pKa = 4.09 GSAQMQMLKK246 pKa = 9.96 IVDD249 pKa = 4.33 PLPDD253 pKa = 3.57 EE254 pKa = 5.03 SGLGQAGTLNIHH266 pKa = 5.98 YY267 pKa = 8.97 LHH269 pKa = 6.91 SSEE272 pKa = 5.7 SYY274 pKa = 10.65 SGWGLHH280 pKa = 5.69 IWGDD284 pKa = 4.04 AIDD287 pKa = 4.39 PSIATTWQAPRR298 pKa = 11.84 PYY300 pKa = 10.99 AEE302 pKa = 5.34 LKK304 pKa = 10.41 DD305 pKa = 5.63 DD306 pKa = 3.81 YY307 pKa = 11.25 AIWQVPIEE315 pKa = 4.49 DD316 pKa = 4.25 ANSAFNFIMHH326 pKa = 6.88 FGDD329 pKa = 4.18 VKK331 pKa = 10.9 STDD334 pKa = 3.24 SDD336 pKa = 3.91 LSIVANSFGTDD347 pKa = 2.05 IWIVQDD353 pKa = 3.24 TATIYY358 pKa = 11.2 NNEE361 pKa = 3.72 TDD363 pKa = 4.57 ARR365 pKa = 11.84 AAYY368 pKa = 10.35 NSLLQNLGNQSANLDD383 pKa = 3.83 LSDD386 pKa = 3.62 VTTTDD391 pKa = 2.91 TSSALADD398 pKa = 3.45 GWVDD402 pKa = 3.31 SAQFAQIYY410 pKa = 8.62 VRR412 pKa = 11.84 AYY414 pKa = 9.96 QDD416 pKa = 3.04 SDD418 pKa = 3.47 GDD420 pKa = 4.24 GVGDD424 pKa = 3.61 IQGLISRR431 pKa = 11.84 LDD433 pKa = 3.54 YY434 pKa = 11.48 LKK436 pKa = 11.17 DD437 pKa = 3.47 NGIDD441 pKa = 4.34 AIWLMPMMEE450 pKa = 4.92 SSDD453 pKa = 3.77 NDD455 pKa = 3.31 HH456 pKa = 7.09 GYY458 pKa = 8.7 ATTDD462 pKa = 3.19 YY463 pKa = 11.01 RR464 pKa = 11.84 KK465 pKa = 9.54 IEE467 pKa = 4.06 SDD469 pKa = 3.69 YY470 pKa = 11.73 GSLDD474 pKa = 3.49 DD475 pKa = 6.43 FKK477 pKa = 11.77 ALLAEE482 pKa = 3.97 AHH484 pKa = 6.07 NRR486 pKa = 11.84 DD487 pKa = 3.21 MAIILDD493 pKa = 3.82 YY494 pKa = 11.09 VINHH498 pKa = 6.33 SSNQNPLFADD508 pKa = 3.76 AMSSANNDD516 pKa = 2.69 KK517 pKa = 10.66 RR518 pKa = 11.84 DD519 pKa = 3.28 WYY521 pKa = 10.02 IIQADD526 pKa = 4.62 KK527 pKa = 10.88 PLGWNIWGGDD537 pKa = 3.59 PWRR540 pKa = 11.84 SSPGGAYY547 pKa = 9.62 YY548 pKa = 10.83 AAFADD553 pKa = 4.08 SMPDD557 pKa = 3.36 FDD559 pKa = 5.03 LTNPDD564 pKa = 3.42 VIAFHH569 pKa = 6.35 QNNLKK574 pKa = 10.08 FWLNMGVDD582 pKa = 4.3 GFRR585 pKa = 11.84 FDD587 pKa = 3.45 AVGVLVEE594 pKa = 4.17 NGKK597 pKa = 9.63 DD598 pKa = 3.33 ALEE601 pKa = 4.57 DD602 pKa = 3.35 QPEE605 pKa = 3.92 NHH607 pKa = 6.79 VIMKK611 pKa = 8.56 TMKK614 pKa = 9.72 DD615 pKa = 3.42 TIEE618 pKa = 4.76 SYY620 pKa = 10.31 DD621 pKa = 3.28 NRR623 pKa = 11.84 YY624 pKa = 8.75 IVCEE628 pKa = 4.18 SPSGYY633 pKa = 10.79 AAFANDD639 pKa = 3.74 NSCGRR644 pKa = 11.84 AFNFSAGHH652 pKa = 7.13 AILGMVDD659 pKa = 3.48 TGVVWDD665 pKa = 4.24 EE666 pKa = 4.55 LLTEE670 pKa = 4.62 LAASNTDD677 pKa = 3.44 QMPMILANHH686 pKa = 6.9 DD687 pKa = 3.6 FFAGDD692 pKa = 3.67 RR693 pKa = 11.84 VWNQLSGNVAQYY705 pKa = 11.24 KK706 pKa = 9.77 LAAATYY712 pKa = 10.02 LLASSNPFTYY722 pKa = 10.32 YY723 pKa = 10.84 GEE725 pKa = 4.62 EE726 pKa = 3.95 IGLAAADD733 pKa = 4.17 NLSGDD738 pKa = 3.32 WSIRR742 pKa = 11.84 TPMSWTDD749 pKa = 3.26 DD750 pKa = 3.52 TNNAGFTTGTPFRR763 pKa = 11.84 DD764 pKa = 3.31 LSSNVASNNVTAQLANADD782 pKa = 3.97 SLLNYY787 pKa = 9.94 YY788 pKa = 10.15 RR789 pKa = 11.84 DD790 pKa = 3.77 LYY792 pKa = 9.54 QVRR795 pKa = 11.84 NDD797 pKa = 3.42 HH798 pKa = 6.48 SVIATGILNVQSSGGDD814 pKa = 3.17 EE815 pKa = 4.07 VLKK818 pKa = 10.6 LVRR821 pKa = 11.84 TGTGTEE827 pKa = 3.69 AVILINYY834 pKa = 9.39 SDD836 pKa = 4.11 GDD838 pKa = 3.61 EE839 pKa = 4.34 SVTVDD844 pKa = 3.43 VTHH847 pKa = 7.51 DD848 pKa = 3.4 NAVYY852 pKa = 10.0 TDD854 pKa = 4.1 EE855 pKa = 6.15 LSDD858 pKa = 4.14 LADD861 pKa = 4.26 AGASSSGQLTVIVPAQSAAVFVLTVANN888 pKa = 4.03
Molecular weight: 96.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.783
IPC_protein 3.834
Toseland 3.605
ProMoST 4.012
Dawson 3.846
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.656
Grimsley 3.503
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.24
Thurlkill 3.656
EMBOSS 3.795
Sillero 3.961
Patrickios 1.227
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|W7QVH6|W7QVH6_9ALTE Uncharacterized protein OS=Catenovulum agarivorans DS-2 OX=1328313 GN=DS2_02830 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 10.14 RR12 pKa = 11.84 KK13 pKa = 9.13 RR14 pKa = 11.84 SHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3835
0
3835
1380918
28
3888
360.1
40.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.67 ± 0.038
0.977 ± 0.013
5.694 ± 0.037
5.869 ± 0.036
4.253 ± 0.022
6.316 ± 0.038
2.23 ± 0.021
6.427 ± 0.03
5.658 ± 0.04
9.978 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.198 ± 0.018
5.031 ± 0.035
3.726 ± 0.024
5.543 ± 0.046
3.955 ± 0.028
6.585 ± 0.04
5.449 ± 0.042
6.742 ± 0.037
1.303 ± 0.017
3.396 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here