Niastella populi

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Niastella

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6536 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V9GDG3|A0A1V9GDG3_9BACT Ferritin OS=Niastella populi OX=550983 GN=A4R26_01455 PE=3 SV=1
MM1 pKa = 7.29YY2 pKa = 9.94SYY4 pKa = 11.68SLLEE8 pKa = 4.09PGCYY12 pKa = 10.1YY13 pKa = 10.81LIQEE17 pKa = 4.79KK18 pKa = 10.27EE19 pKa = 4.25DD20 pKa = 3.75EE21 pKa = 4.46PVTLVKK27 pKa = 10.7IMVEE31 pKa = 4.29TDD33 pKa = 2.32QCMYY37 pKa = 8.25VTQYY41 pKa = 11.76EE42 pKa = 4.46DD43 pKa = 3.49MPVLAWKK50 pKa = 10.03KK51 pKa = 10.44KK52 pKa = 9.17NDD54 pKa = 4.5PIFDD58 pKa = 3.82ILEE61 pKa = 4.24LLSDD65 pKa = 4.05EE66 pKa = 5.65KK67 pKa = 11.25VKK69 pKa = 10.58EE70 pKa = 3.63WDD72 pKa = 3.11AVYY75 pKa = 11.04NDD77 pKa = 3.99SQDD80 pKa = 3.11AYY82 pKa = 11.28YY83 pKa = 10.66EE84 pKa = 4.25EE85 pKa = 5.2EE86 pKa = 4.6DD87 pKa = 5.05DD88 pKa = 4.32EE89 pKa = 4.61

Molecular weight: 10.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V9FKD7|A0A1V9FKD7_9BACT Uncharacterized protein OS=Niastella populi OX=550983 GN=A4R26_22700 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.18RR3 pKa = 11.84QAASGKK9 pKa = 9.34RR10 pKa = 11.84QAASGKK16 pKa = 9.34RR17 pKa = 11.84QAASGKK23 pKa = 9.34RR24 pKa = 11.84QAASGKK30 pKa = 9.34RR31 pKa = 11.84QAASGKK37 pKa = 9.34RR38 pKa = 11.84QAASGKK44 pKa = 9.34RR45 pKa = 11.84QAASGKK51 pKa = 9.34RR52 pKa = 11.84QAASGKK58 pKa = 9.34RR59 pKa = 11.84QAASGKK65 pKa = 9.34RR66 pKa = 11.84QAASGKK72 pKa = 9.34RR73 pKa = 11.84QAASGKK79 pKa = 9.34RR80 pKa = 11.84QAASGKK86 pKa = 9.34RR87 pKa = 11.84QAASGKK93 pKa = 9.34RR94 pKa = 11.84QAASGKK100 pKa = 9.34RR101 pKa = 11.84QAASGKK107 pKa = 9.34RR108 pKa = 11.84QAASGKK114 pKa = 9.34RR115 pKa = 11.84QAASGKK121 pKa = 9.34RR122 pKa = 11.84QAASGKK128 pKa = 9.34RR129 pKa = 11.84QAASGKK135 pKa = 9.34RR136 pKa = 11.84QAASGKK142 pKa = 9.34RR143 pKa = 11.84QAASGKK149 pKa = 9.34RR150 pKa = 11.84QAASGKK156 pKa = 9.34RR157 pKa = 11.84QAASGKK163 pKa = 9.34RR164 pKa = 11.84QAASGKK170 pKa = 9.34RR171 pKa = 11.84QAASGKK177 pKa = 9.34RR178 pKa = 11.84QAASGKK184 pKa = 9.34RR185 pKa = 11.84QAASGKK191 pKa = 9.34RR192 pKa = 11.84QAASGKK198 pKa = 9.34RR199 pKa = 11.84QAASGKK205 pKa = 9.34RR206 pKa = 11.84QAASGKK212 pKa = 9.34RR213 pKa = 11.84QAASGKK219 pKa = 9.4RR220 pKa = 11.84QAASGNNFGRR230 pKa = 11.84RR231 pKa = 11.84KK232 pKa = 9.64VVNKK236 pKa = 9.84IPNFASRR243 pKa = 11.84FTFHH247 pKa = 7.46FARR250 pKa = 11.84PFTIHH255 pKa = 6.61ARR257 pKa = 11.84YY258 pKa = 8.95NQFNN262 pKa = 3.54

Molecular weight: 27.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6536

0

6536

2361176

26

4087

361.3

40.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.75 ± 0.034

0.888 ± 0.011

5.079 ± 0.019

5.375 ± 0.036

4.723 ± 0.023

7.087 ± 0.035

1.932 ± 0.016

6.733 ± 0.024

6.487 ± 0.032

9.105 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.014

5.736 ± 0.034

4.061 ± 0.02

3.925 ± 0.02

4.392 ± 0.021

6.12 ± 0.028

6.17 ± 0.035

6.551 ± 0.025

1.373 ± 0.011

4.193 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski