Bean yellow disorder virus
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B2BZW8|B2BZW8_9CLOS p60 OS=Bean yellow disorder virus OX=267970 PE=4 SV=1
MM1 pKa = 7.36 GLKK4 pKa = 9.87 IVIYY8 pKa = 8.2 FHH10 pKa = 6.63 NCGLIIYY17 pKa = 8.19 YY18 pKa = 10.41 CADD21 pKa = 3.17 NRR23 pKa = 11.84 DD24 pKa = 3.51 FLGDD28 pKa = 3.54 FEE30 pKa = 4.79 VVRR33 pKa = 11.84 TEE35 pKa = 4.53 DD36 pKa = 3.66 FSEE39 pKa = 4.55 LSDD42 pKa = 4.23 IITNFPFVKK51 pKa = 10.13 TSWW54 pKa = 3.13
Molecular weight: 6.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.14
IPC2_protein 4.533
IPC_protein 4.304
Toseland 4.126
ProMoST 4.469
Dawson 4.291
Bjellqvist 4.457
Wikipedia 4.228
Rodwell 4.151
Grimsley 4.05
Solomon 4.279
Lehninger 4.24
Nozaki 4.431
DTASelect 4.622
Thurlkill 4.19
EMBOSS 4.24
Sillero 4.431
Patrickios 2.015
IPC_peptide 4.279
IPC2_peptide 4.418
IPC2.peptide.svr19 4.348
Protein with the highest isoelectric point:
>tr|B2BZW4|B2BZW4_9CLOS p6 OS=Bean yellow disorder virus OX=267970 PE=4 SV=1
MM1 pKa = 7.56 GINYY5 pKa = 9.19 GFLSHH10 pKa = 6.96 ACNRR14 pKa = 11.84 NGILYY19 pKa = 8.92 NNSMCVVDD27 pKa = 6.48 IIGFWLTVCVLIIILIYY44 pKa = 10.81 LLFICFVKK52 pKa = 10.14 FVIKK56 pKa = 10.52 II57 pKa = 3.59
Molecular weight: 6.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.766
IPC2_protein 7.439
IPC_protein 7.527
Toseland 6.942
ProMoST 7.922
Dawson 8.097
Bjellqvist 8.463
Wikipedia 8.009
Rodwell 8.083
Grimsley 6.883
Solomon 8.258
Lehninger 8.287
Nozaki 8.77
DTASelect 8.2
Thurlkill 8.229
EMBOSS 8.331
Sillero 8.609
Patrickios 0.223
IPC_peptide 8.258
IPC2_peptide 8.141
IPC2.peptide.svr19 8.08
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13
0
13
5080
48
2021
390.8
44.94
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.878 ± 0.252
2.087 ± 0.239
5.965 ± 0.282
5.591 ± 0.483
5.866 ± 0.608
5.039 ± 0.293
2.146 ± 0.295
6.811 ± 0.289
7.776 ± 0.453
9.528 ± 0.377
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.697 ± 0.27
7.185 ± 0.378
3.406 ± 0.25
2.5 ± 0.214
4.862 ± 0.4
7.52 ± 0.399
5.02 ± 0.242
6.831 ± 0.626
0.807 ± 0.105
4.488 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here