Oreochromis aureus (Israeli tilapia) (Chromis aureus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; A

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58969 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A668SHA4|A0A668SHA4_OREAU Isoform of A0A668SHA0 F-actin-capping protein subunit beta OS=Oreochromis aureus OX=47969 PE=3 SV=1
MM1 pKa = 7.71LLPLSALSGTSLFGGDD17 pKa = 3.21IRR19 pKa = 11.84QVHH22 pKa = 4.91PTITEE27 pKa = 3.85EE28 pKa = 4.25PLEE31 pKa = 4.64DD32 pKa = 3.5DD33 pKa = 3.2HH34 pKa = 9.88DD35 pKa = 4.05EE36 pKa = 4.3FEE38 pKa = 4.56EE39 pKa = 4.94VDD41 pKa = 5.86FEE43 pKa = 5.76DD44 pKa = 6.29LDD46 pKa = 4.04DD47 pKa = 4.57CRR49 pKa = 11.84SIASDD54 pKa = 3.53DD55 pKa = 3.73SFYY58 pKa = 10.96PPDD61 pKa = 4.14DD62 pKa = 3.52VFADD66 pKa = 4.16SEE68 pKa = 4.45RR69 pKa = 11.84SPSPDD74 pKa = 3.04SPEE77 pKa = 3.99PLSFFQACCTNNAAIVRR94 pKa = 11.84IMIRR98 pKa = 11.84HH99 pKa = 4.88GVKK102 pKa = 10.23EE103 pKa = 4.22EE104 pKa = 4.01EE105 pKa = 4.48VKK107 pKa = 9.9EE108 pKa = 3.95TDD110 pKa = 3.01RR111 pKa = 11.84NNRR114 pKa = 11.84LGLLVACYY122 pKa = 9.68QGYY125 pKa = 9.67VDD127 pKa = 5.2VVIALSQCPYY137 pKa = 11.12LDD139 pKa = 4.55VNWQDD144 pKa = 3.88SEE146 pKa = 4.93GNTALITAAQAVTSVICPPVPVTFVSCSLPPIVGG180 pKa = 3.27

Molecular weight: 19.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A668TMV2|A0A668TMV2_OREAU Uncharacterized protein OS=Oreochromis aureus OX=47969 PE=3 SV=1
SS1 pKa = 6.75SSSPAAASSSLPAAVFPPCLHH22 pKa = 6.82PRR24 pKa = 11.84LAQPPCLHH32 pKa = 6.81PRR34 pKa = 11.84LVQPPCLHH42 pKa = 6.27PRR44 pKa = 11.84RR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84QLHH50 pKa = 5.59HH51 pKa = 6.24PRR53 pKa = 11.84PRR55 pKa = 11.84RR56 pKa = 11.84QHH58 pKa = 5.43QLHH61 pKa = 6.51HH62 pKa = 6.14PRR64 pKa = 11.84PRR66 pKa = 11.84RR67 pKa = 11.84QHH69 pKa = 5.43QLHH72 pKa = 6.51HH73 pKa = 6.14PRR75 pKa = 11.84PRR77 pKa = 11.84RR78 pKa = 11.84QHH80 pKa = 5.43QLHH83 pKa = 6.51HH84 pKa = 6.14PRR86 pKa = 11.84PRR88 pKa = 11.84RR89 pKa = 11.84QHH91 pKa = 5.43QLHH94 pKa = 6.51HH95 pKa = 6.14PRR97 pKa = 11.84PRR99 pKa = 11.84RR100 pKa = 11.84QHH102 pKa = 5.43QLHH105 pKa = 6.51HH106 pKa = 6.14PRR108 pKa = 11.84PRR110 pKa = 11.84RR111 pKa = 11.84QHH113 pKa = 5.43QLHH116 pKa = 6.51HH117 pKa = 6.14PRR119 pKa = 11.84PRR121 pKa = 11.84RR122 pKa = 11.84QHH124 pKa = 5.43QLHH127 pKa = 6.51HH128 pKa = 6.14PRR130 pKa = 11.84PRR132 pKa = 11.84RR133 pKa = 11.84QHH135 pKa = 5.43QLHH138 pKa = 6.49HH139 pKa = 6.07PRR141 pKa = 11.84PRR143 pKa = 11.84RR144 pKa = 11.84RR145 pKa = 11.84RR146 pKa = 11.84QLHH149 pKa = 5.59HH150 pKa = 6.24PRR152 pKa = 11.84PRR154 pKa = 11.84RR155 pKa = 11.84QHH157 pKa = 5.43QLHH160 pKa = 6.51HH161 pKa = 6.14PRR163 pKa = 11.84PRR165 pKa = 11.84RR166 pKa = 11.84QHH168 pKa = 5.43QLHH171 pKa = 6.59HH172 pKa = 6.71PRR174 pKa = 11.84PQRR177 pKa = 11.84QHH179 pKa = 5.55QPHH182 pKa = 7.24PRR184 pKa = 11.84PQQQLQHH191 pKa = 5.8HH192 pKa = 6.28HH193 pKa = 6.4LRR195 pKa = 11.84WLRR198 pKa = 11.84LARR201 pKa = 11.84PPLQLQPRR209 pKa = 11.84LAQPPLQLQPRR220 pKa = 11.84LAQPPLQLRR229 pKa = 11.84FSSSLGLRR237 pKa = 11.84FGPGPVPPSWTPYY250 pKa = 9.18RR251 pKa = 11.84PPGSGVFLWLSGASPP266 pKa = 3.75

Molecular weight: 32.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24412

34557

58969

37318939

18

8615

632.9

70.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.461 ± 0.008

2.287 ± 0.008

5.229 ± 0.007

6.82 ± 0.011

3.901 ± 0.006

6.134 ± 0.01

2.619 ± 0.004

4.777 ± 0.007

5.834 ± 0.009

9.769 ± 0.012

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.004

3.994 ± 0.005

5.275 ± 0.01

4.635 ± 0.009

5.454 ± 0.007

8.19 ± 0.01

5.575 ± 0.006

6.459 ± 0.007

1.196 ± 0.003

2.952 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski