Amino acid dipepetide frequency for Oreochromis aureus (Israeli tilapia) (Chromis aureus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.8AlaAla: 5.8 ± 0.022	1.31AlaCys: 1.31 ± 0.007	3.165AlaAsp: 3.165 ± 0.01	4.553AlaGlu: 4.553 ± 0.016	2.5AlaPhe: 2.5 ± 0.01	4.065AlaGly: 4.065 ± 0.014	1.477AlaHis: 1.477 ± 0.007	2.964AlaIle: 2.964 ± 0.011	3.508AlaLys: 3.508 ± 0.013	6.562AlaLeu: 6.562 ± 0.018	1.65AlaMet: 1.65 ± 0.008	2.252AlaAsn: 2.252 ± 0.009	3.068AlaPro: 3.068 ± 0.014	2.875AlaGln: 2.875 ± 0.01	3.068AlaArg: 3.068 ± 0.01	5.131AlaSer: 5.131 ± 0.013	3.408AlaThr: 3.408 ± 0.011	4.949AlaVal: 4.949 ± 0.015	0.658AlaTrp: 0.658 ± 0.004	1.647AlaTyr: 1.647 ± 0.008	0.0AlaXaa: 0.0 ± 0.0
Cys	1.204CysAla: 1.204 ± 0.007	0.652CysCys: 0.652 ± 0.006	1.163CysAsp: 1.163 ± 0.01	1.25CysGlu: 1.25 ± 0.007	1.01CysPhe: 1.01 ± 0.006	1.505CysGly: 1.505 ± 0.01	0.672CysHis: 0.672 ± 0.005	1.117CysIle: 1.117 ± 0.007	1.217CysLys: 1.217 ± 0.008	2.287CysLeu: 2.287 ± 0.009	0.491CysMet: 0.491 ± 0.004	0.873CysAsn: 0.873 ± 0.006	1.228CysPro: 1.228 ± 0.01	1.031CysGln: 1.031 ± 0.007	1.27CysArg: 1.27 ± 0.007	2.06CysSer: 2.06 ± 0.012	1.231CysThr: 1.231 ± 0.007	1.637CysVal: 1.637 ± 0.009	0.302CysTrp: 0.302 ± 0.003	0.671CysTyr: 0.671 ± 0.005	0.0CysXaa: 0.0 ± 0.0
Asp	2.952AspAla: 2.952 ± 0.01	1.167AspCys: 1.167 ± 0.009	3.081AspAsp: 3.081 ± 0.013	3.743AspGlu: 3.743 ± 0.014	2.263AspPhe: 2.263 ± 0.009	3.605AspGly: 3.605 ± 0.013	1.216AspHis: 1.216 ± 0.006	2.931AspIle: 2.931 ± 0.011	2.859AspLys: 2.859 ± 0.01	5.125AspLeu: 5.125 ± 0.013	1.318AspMet: 1.318 ± 0.006	2.024AspAsn: 2.024 ± 0.01	2.84AspPro: 2.84 ± 0.01	1.951AspGln: 1.951 ± 0.009	2.649AspArg: 2.649 ± 0.01	4.215AspSer: 4.215 ± 0.013	2.644AspThr: 2.644 ± 0.008	3.331AspVal: 3.331 ± 0.01	0.709AspTrp: 0.709 ± 0.006	1.664AspTyr: 1.664 ± 0.008	0.001AspXaa: 0.001 ± 0.0
Glu	4.722GluAla: 4.722 ± 0.015	1.285GluCys: 1.285 ± 0.01	4.278GluAsp: 4.278 ± 0.013	7.218GluGlu: 7.218 ± 0.032	2.119GluPhe: 2.119 ± 0.007	4.068GluGly: 4.068 ± 0.013	1.463GluHis: 1.463 ± 0.007	3.084GluIle: 3.084 ± 0.011	4.813GluLys: 4.813 ± 0.019	6.38GluLeu: 6.38 ± 0.019	1.807GluMet: 1.807 ± 0.009	2.87GluAsn: 2.87 ± 0.009	2.6GluPro: 2.6 ± 0.01	3.045GluGln: 3.045 ± 0.012	4.113GluArg: 4.113 ± 0.017	4.159GluSer: 4.159 ± 0.013	3.34GluThr: 3.34 ± 0.011	4.367GluVal: 4.367 ± 0.013	0.729GluTrp: 0.729 ± 0.005	1.743GluTyr: 1.743 ± 0.007	0.0GluXaa: 0.0 ± 0.0
Phe	2.085PheAla: 2.085 ± 0.009	1.036PheCys: 1.036 ± 0.006	1.914PheAsp: 1.914 ± 0.008	2.03PheGlu: 2.03 ± 0.007	1.895PhePhe: 1.895 ± 0.009	2.332PheGly: 2.332 ± 0.01	1.085PheHis: 1.085 ± 0.006	2.168PheIle: 2.168 ± 0.01	1.99PheLys: 1.99 ± 0.008	4.219PheLeu: 4.219 ± 0.015	0.892PheMet: 0.892 ± 0.005	1.633PheAsn: 1.633 ± 0.007	1.855PhePro: 1.855 ± 0.009	1.706PheGln: 1.706 ± 0.007	1.908PheArg: 1.908 ± 0.007	3.499PheSer: 3.499 ± 0.011	2.453PheThr: 2.453 ± 0.01	2.441PheVal: 2.441 ± 0.011	0.511PheTrp: 0.511 ± 0.004	1.355PheTyr: 1.355 ± 0.007	0.0PheXaa: 0.0 ± 0.0
Gly	3.819GlyAla: 3.819 ± 0.015	1.212GlyCys: 1.212 ± 0.007	3.19GlyAsp: 3.19 ± 0.011	3.98GlyGlu: 3.98 ± 0.014	2.479GlyPhe: 2.479 ± 0.011	4.827GlyGly: 4.827 ± 0.02	1.624GlyHis: 1.624 ± 0.008	2.792GlyIle: 2.792 ± 0.01	3.715GlyLys: 3.715 ± 0.013	5.378GlyLeu: 5.378 ± 0.015	1.511GlyMet: 1.511 ± 0.007	2.491GlyAsn: 2.491 ± 0.009	2.939GlyPro: 2.939 ± 0.022	2.652GlyGln: 2.652 ± 0.009	3.431GlyArg: 3.431 ± 0.014	5.242GlySer: 5.242 ± 0.016	3.389GlyThr: 3.389 ± 0.013	3.969GlyVal: 3.969 ± 0.013	0.778GlyTrp: 0.778 ± 0.005	1.925GlyTyr: 1.925 ± 0.009	0.0GlyXaa: 0.0 ± 0.0
His	1.346HisAla: 1.346 ± 0.007	0.778HisCys: 0.778 ± 0.005	1.011HisAsp: 1.011 ± 0.006	1.282HisGlu: 1.282 ± 0.007	1.131HisPhe: 1.131 ± 0.006	1.502HisGly: 1.502 ± 0.008	0.965HisHis: 0.965 ± 0.007	1.42HisIle: 1.42 ± 0.006	1.325HisLys: 1.325 ± 0.006	2.735HisLeu: 2.735 ± 0.01	0.663HisMet: 0.663 ± 0.005	1.058HisAsn: 1.058 ± 0.005	1.504HisPro: 1.504 ± 0.008	1.203HisGln: 1.203 ± 0.007	1.585HisArg: 1.585 ± 0.007	2.33HisSer: 2.33 ± 0.011	1.633HisThr: 1.633 ± 0.009	1.483HisVal: 1.483 ± 0.007	0.339HisTrp: 0.339 ± 0.003	0.894HisTyr: 0.894 ± 0.005	0.0HisXaa: 0.0 ± 0.0
Ile	2.782IleAla: 2.782 ± 0.01	1.176IleCys: 1.176 ± 0.007	2.328IleAsp: 2.328 ± 0.009	2.609IleGlu: 2.609 ± 0.01	2.075IlePhe: 2.075 ± 0.01	2.471IleGly: 2.471 ± 0.008	1.391IleHis: 1.391 ± 0.008	2.725IleIle: 2.725 ± 0.011	2.838IleLys: 2.838 ± 0.01	4.693IleLeu: 4.693 ± 0.015	1.177IleMet: 1.177 ± 0.006	2.167IleAsn: 2.167 ± 0.009	2.519IlePro: 2.519 ± 0.01	2.368IleGln: 2.368 ± 0.009	2.605IleArg: 2.605 ± 0.009	3.927IleSer: 3.927 ± 0.011	2.976IleThr: 2.976 ± 0.011	2.82IleVal: 2.82 ± 0.011	0.526IleTrp: 0.526 ± 0.004	1.597IleTyr: 1.597 ± 0.007	0.0IleXaa: 0.0 ± 0.0
Lys	3.859LysAla: 3.859 ± 0.013	1.098LysCys: 1.098 ± 0.007	3.368LysAsp: 3.368 ± 0.011	4.86LysGlu: 4.86 ± 0.017	1.802LysPhe: 1.802 ± 0.008	3.26LysGly: 3.26 ± 0.012	1.473LysHis: 1.473 ± 0.007	2.77LysIle: 2.77 ± 0.01	4.583LysLys: 4.583 ± 0.02	5.367LysLeu: 5.367 ± 0.016	1.543LysMet: 1.543 ± 0.007	2.506LysAsn: 2.506 ± 0.009	2.777LysPro: 2.777 ± 0.011	2.647LysGln: 2.647 ± 0.013	3.336LysArg: 3.336 ± 0.01	3.789LysSer: 3.789 ± 0.014	3.258LysThr: 3.258 ± 0.011	3.758LysVal: 3.758 ± 0.011	0.617LysTrp: 0.617 ± 0.005	1.665LysTyr: 1.665 ± 0.007	0.0LysXaa: 0.0 ± 0.0
Leu	6.201LeuAla: 6.201 ± 0.016	2.345LeuCys: 2.345 ± 0.009	4.963LeuAsp: 4.963 ± 0.011	6.61LeuGlu: 6.61 ± 0.023	3.778LeuPhe: 3.778 ± 0.014	5.183LeuGly: 5.183 ± 0.014	2.787LeuHis: 2.787 ± 0.011	4.21LeuIle: 4.21 ± 0.013	5.936LeuLys: 5.936 ± 0.016	10.325LeuLeu: 10.325 ± 0.026	2.234LeuMet: 2.234 ± 0.009	3.848LeuAsn: 3.848 ± 0.012	5.067LeuPro: 5.067 ± 0.014	5.483LeuGln: 5.483 ± 0.018	5.611LeuArg: 5.611 ± 0.017	8.117LeuSer: 8.117 ± 0.019	5.407LeuThr: 5.407 ± 0.015	5.612LeuVal: 5.612 ± 0.015	1.109LeuTrp: 1.109 ± 0.006	2.862LeuTyr: 2.862 ± 0.01	0.0LeuXaa: 0.0 ± 0.0
Met	1.901MetAla: 1.901 ± 0.009	0.525MetCys: 0.525 ± 0.004	1.45MetAsp: 1.45 ± 0.006	2.015MetGlu: 2.015 ± 0.009	0.941MetPhe: 0.941 ± 0.004	1.421MetGly: 1.421 ± 0.007	0.508MetHis: 0.508 ± 0.004	0.99MetIle: 0.99 ± 0.006	1.573MetLys: 1.573 ± 0.007	2.204MetLeu: 2.204 ± 0.008	0.723MetMet: 0.723 ± 0.004	0.976MetAsn: 0.976 ± 0.006	1.069MetPro: 1.069 ± 0.006	1.01MetGln: 1.01 ± 0.006	1.21MetArg: 1.21 ± 0.006	1.836MetSer: 1.836 ± 0.007	1.316MetThr: 1.316 ± 0.006	1.624MetVal: 1.624 ± 0.007	0.29MetTrp: 0.29 ± 0.002	0.688MetTyr: 0.688 ± 0.004	0.0MetXaa: 0.0 ± 0.0
Asn	2.253AsnAla: 2.253 ± 0.01	0.919AsnCys: 0.919 ± 0.006	1.824AsnAsp: 1.824 ± 0.008	2.216AsnGlu: 2.216 ± 0.009	1.564AsnPhe: 1.564 ± 0.006	2.761AsnGly: 2.761 ± 0.011	1.027AsnHis: 1.027 ± 0.005	2.38AsnIle: 2.38 ± 0.009	2.388AsnLys: 2.388 ± 0.008	3.862AsnLeu: 3.862 ± 0.012	1.093AsnMet: 1.093 ± 0.006	1.893AsnAsn: 1.893 ± 0.009	2.302AsnPro: 2.302 ± 0.009	1.751AsnGln: 1.751 ± 0.008	2.022AsnArg: 2.022 ± 0.008	3.207AsnSer: 3.207 ± 0.011	2.348AsnThr: 2.348 ± 0.009	2.387AsnVal: 2.387 ± 0.009	0.485AsnTrp: 0.485 ± 0.004	1.256AsnTyr: 1.256 ± 0.006	0.0AsnXaa: 0.0 ± 0.0
Pro	3.672ProAla: 3.672 ± 0.014	0.999ProCys: 0.999 ± 0.007	2.812ProAsp: 2.812 ± 0.01	3.633ProGlu: 3.633 ± 0.011	1.804ProPhe: 1.804 ± 0.009	3.659ProGly: 3.659 ± 0.023	1.414ProHis: 1.414 ± 0.009	1.949ProIle: 1.949 ± 0.008	2.454ProLys: 2.454 ± 0.01	4.649ProLeu: 4.649 ± 0.014	0.994ProMet: 0.994 ± 0.006	1.917ProAsn: 1.917 ± 0.008	4.577ProPro: 4.577 ± 0.026	2.423ProGln: 2.423 ± 0.012	2.504ProArg: 2.504 ± 0.013	4.904ProSer: 4.904 ± 0.017	2.854ProThr: 2.854 ± 0.012	3.548ProVal: 3.548 ± 0.012	0.547ProTrp: 0.547 ± 0.004	1.434ProTyr: 1.434 ± 0.007	0.0ProXaa: 0.0 ± 0.0
Gln	3.157GlnAla: 3.157 ± 0.012	1.002GlnCys: 1.002 ± 0.008	2.264GlnAsp: 2.264 ± 0.008	3.414GlnGlu: 3.414 ± 0.013	1.47GlnPhe: 1.47 ± 0.006	2.63GlnGly: 2.63 ± 0.011	1.3GlnHis: 1.3 ± 0.007	2.079GlnIle: 2.079 ± 0.008	2.673GlnLys: 2.673 ± 0.01	4.586GlnLeu: 4.586 ± 0.015	1.174GlnMet: 1.174 ± 0.006	1.784GlnAsn: 1.784 ± 0.007	2.315GlnPro: 2.315 ± 0.011	3.046GlnGln: 3.046 ± 0.019	2.931GlnArg: 2.931 ± 0.011	3.268GlnSer: 3.268 ± 0.013	2.466GlnThr: 2.466 ± 0.012	2.877GlnVal: 2.877 ± 0.009	0.57GlnTrp: 0.57 ± 0.004	1.343GlnTyr: 1.343 ± 0.006	0.0GlnXaa: 0.0 ± 0.0
Arg	3.323ArgAla: 3.323 ± 0.009	1.244ArgCys: 1.244 ± 0.008	2.912ArgAsp: 2.912 ± 0.011	3.767ArgGlu: 3.767 ± 0.015	2.053ArgPhe: 2.053 ± 0.009	3.194ArgGly: 3.194 ± 0.014	1.494ArgHis: 1.494 ± 0.007	2.537ArgIle: 2.537 ± 0.008	3.497ArgLys: 3.497 ± 0.011	5.336ArgLeu: 5.336 ± 0.015	1.313ArgMet: 1.313 ± 0.006	2.185ArgAsn: 2.185 ± 0.008	2.657ArgPro: 2.657 ± 0.012	2.6ArgGln: 2.6 ± 0.01	3.874ArgArg: 3.874 ± 0.016	4.113ArgSer: 4.113 ± 0.014	2.792ArgThr: 2.792 ± 0.009	3.336ArgVal: 3.336 ± 0.01	0.678ArgTrp: 0.678 ± 0.005	1.636ArgTyr: 1.636 ± 0.007	0.0ArgXaa: 0.0 ± 0.0
Ser	5.196SerAla: 5.196 ± 0.014	1.919SerCys: 1.919 ± 0.01	4.16SerAsp: 4.16 ± 0.013	4.714SerGlu: 4.714 ± 0.014	3.202SerPhe: 3.202 ± 0.011	5.25SerGly: 5.25 ± 0.015	2.154SerHis: 2.154 ± 0.01	3.489SerIle: 3.489 ± 0.011	4.006SerLys: 4.006 ± 0.012	8.072SerLeu: 8.072 ± 0.018	1.773SerMet: 1.773 ± 0.008	2.978SerAsn: 2.978 ± 0.009	5.142SerPro: 5.142 ± 0.022	3.664SerGln: 3.664 ± 0.013	4.141SerArg: 4.141 ± 0.014	9.012SerSer: 9.012 ± 0.035	4.527SerThr: 4.527 ± 0.013	5.313SerVal: 5.313 ± 0.014	0.987SerTrp: 0.987 ± 0.006	2.201SerTyr: 2.201 ± 0.009	0.0SerXaa: 0.0 ± 0.0
Thr	3.926ThrAla: 3.926 ± 0.01	1.385ThrCys: 1.385 ± 0.011	2.913ThrAsp: 2.913 ± 0.009	3.705ThrGlu: 3.705 ± 0.011	2.233ThrPhe: 2.233 ± 0.008	3.687ThrGly: 3.687 ± 0.013	1.42ThrHis: 1.42 ± 0.008	2.604ThrIle: 2.604 ± 0.009	2.773ThrLys: 2.773 ± 0.011	5.497ThrLeu: 5.497 ± 0.014	1.246ThrMet: 1.246 ± 0.006	2.012ThrAsn: 2.012 ± 0.007	3.387ThrPro: 3.387 ± 0.013	2.334ThrGln: 2.334 ± 0.009	2.442ThrArg: 2.442 ± 0.008	4.542ThrSer: 4.542 ± 0.015	3.199ThrThr: 3.199 ± 0.017	4.23ThrVal: 4.23 ± 0.013	0.682ThrTrp: 0.682 ± 0.005	1.536ThrTyr: 1.536 ± 0.008	0.0ThrXaa: 0.0 ± 0.0
Val	4.19ValAla: 4.19 ± 0.012	1.779ValCys: 1.779 ± 0.01	3.311ValAsp: 3.311 ± 0.011	4.092ValGlu: 4.092 ± 0.01	2.861ValPhe: 2.861 ± 0.011	3.51ValGly: 3.51 ± 0.011	1.597ValHis: 1.597 ± 0.007	3.287ValIle: 3.287 ± 0.012	3.772ValLys: 3.772 ± 0.01	6.304ValLeu: 6.304 ± 0.017	1.575ValMet: 1.575 ± 0.008	2.613ValAsn: 2.613 ± 0.01	3.131ValPro: 3.131 ± 0.01	2.751ValGln: 2.751 ± 0.01	3.35ValArg: 3.35 ± 0.011	5.232ValSer: 5.232 ± 0.014	4.037ValThr: 4.037 ± 0.012	4.457ValVal: 4.457 ± 0.013	0.81ValTrp: 0.81 ± 0.005	1.932ValTyr: 1.932 ± 0.008	0.001ValXaa: 0.001 ± 0.0
Trp	0.693TrpAla: 0.693 ± 0.005	0.275TrpCys: 0.275 ± 0.003	0.674TrpAsp: 0.674 ± 0.005	0.78TrpGlu: 0.78 ± 0.004	0.483TrpPhe: 0.483 ± 0.004	0.632TrpGly: 0.632 ± 0.005	0.285TrpHis: 0.285 ± 0.003	0.608TrpIle: 0.608 ± 0.005	0.766TrpLys: 0.766 ± 0.005	1.203TrpLeu: 1.203 ± 0.007	0.361TrpMet: 0.361 ± 0.003	0.548TrpAsn: 0.548 ± 0.004	0.44TrpPro: 0.44 ± 0.004	0.512TrpGln: 0.512 ± 0.004	0.724TrpArg: 0.724 ± 0.005	0.943TrpSer: 0.943 ± 0.006	0.709TrpThr: 0.709 ± 0.005	0.731TrpVal: 0.731 ± 0.004	0.213TrpTrp: 0.213 ± 0.003	0.381TrpTyr: 0.381 ± 0.003	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.53TyrAla: 1.53 ± 0.006	0.765TyrCys: 0.765 ± 0.004	1.505TyrAsp: 1.505 ± 0.008	1.732TyrGlu: 1.732 ± 0.008	1.344TyrPhe: 1.344 ± 0.006	1.783TyrGly: 1.783 ± 0.008	0.834TyrHis: 0.834 ± 0.005	1.665TyrIle: 1.665 ± 0.008	1.652TyrLys: 1.652 ± 0.008	2.903TyrLeu: 2.903 ± 0.01	0.731TyrMet: 0.731 ± 0.005	1.31TyrAsn: 1.31 ± 0.006	1.33TyrPro: 1.33 ± 0.008	1.3TyrGln: 1.3 ± 0.007	1.764TyrArg: 1.764 ± 0.008	2.375TyrSer: 2.375 ± 0.009	1.767TyrThr: 1.767 ± 0.008	1.72TyrVal: 1.72 ± 0.007	0.42TyrTrp: 0.42 ± 0.005	1.094TyrTyr: 1.094 ± 0.007	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.109XaaXaa: 0.109 ± 0.024

Statistics based on 58969 proteins (37318939 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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