Dialister succinatiphilus YIT 11850
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2140 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H1D2W2|H1D2W2_9FIRM Uncharacterized protein OS=Dialister succinatiphilus YIT 11850 OX=742743 GN=HMPREF9453_01950 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.24 KK3 pKa = 10.19 YY4 pKa = 9.87 ICKK7 pKa = 9.17 ICGWIYY13 pKa = 11.18 DD14 pKa = 4.17 EE15 pKa = 5.52 AQGDD19 pKa = 4.38 PDD21 pKa = 3.97 NGIAPGTKK29 pKa = 9.9 FEE31 pKa = 5.53 DD32 pKa = 3.69 LPADD36 pKa = 4.59 YY37 pKa = 10.21 ICPMCGAGKK46 pKa = 10.3 DD47 pKa = 3.56 EE48 pKa = 4.27 FEE50 pKa = 4.51 EE51 pKa = 4.83 YY52 pKa = 10.52 EE53 pKa = 4.25 GG54 pKa = 4.34
Molecular weight: 6.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.921
IPC2_protein 4.126
IPC_protein 3.999
Toseland 3.808
ProMoST 4.151
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.897
Rodwell 3.834
Grimsley 3.732
Solomon 3.948
Lehninger 3.91
Nozaki 4.101
DTASelect 4.266
Thurlkill 3.859
EMBOSS 3.897
Sillero 4.113
Patrickios 0.477
IPC_peptide 3.948
IPC2_peptide 4.088
IPC2.peptide.svr19 4.014
Protein with the highest isoelectric point:
>tr|H1D358|H1D358_9FIRM Ribosomal RNA small subunit methyltransferase G OS=Dialister succinatiphilus YIT 11850 OX=742743 GN=rsmG PE=3 SV=1
MM1 pKa = 7.7 AKK3 pKa = 7.99 MTFQPNNHH11 pKa = 5.63 WRR13 pKa = 11.84 KK14 pKa = 6.87 QTHH17 pKa = 5.61 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MKK24 pKa = 8.64 TKK26 pKa = 10.33 AGRR29 pKa = 11.84 IVLKK33 pKa = 10.36 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 9.89 GRR40 pKa = 11.84 KK41 pKa = 8.67 VLSAA45 pKa = 4.05
Molecular weight: 5.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.647
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.369
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.095
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2140
0
2140
646481
20
2249
302.1
33.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.447 ± 0.054
1.204 ± 0.022
5.697 ± 0.047
6.824 ± 0.052
4.15 ± 0.04
7.731 ± 0.054
2.307 ± 0.023
6.526 ± 0.039
6.613 ± 0.047
9.361 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.226 ± 0.026
3.537 ± 0.044
3.974 ± 0.031
2.795 ± 0.031
5.185 ± 0.048
5.948 ± 0.043
5.149 ± 0.041
6.704 ± 0.045
1.056 ± 0.019
3.568 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here