Actinokineospora spheciospongiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Actinokineospora

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6610 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W7IYI6|W7IYI6_9PSEU Putative LINOLEOYL-CoA DESATURASE (DELTA(6)-DESATURASE) OS=Actinokineospora spheciospongiae OX=909613 GN=UO65_5621 PE=4 SV=1
MM1 pKa = 7.74IIAIDD6 pKa = 3.81NGDD9 pKa = 4.26LSHH12 pKa = 7.03CSWSTDD18 pKa = 3.1SGLLTFDD25 pKa = 3.24SGRR28 pKa = 11.84WCLRR32 pKa = 11.84TLEE35 pKa = 4.28MVGDD39 pKa = 4.28PDD41 pKa = 4.53QPCLSATPTPTSS53 pKa = 3.17

Molecular weight: 5.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W7ILR2|W7ILR2_9PSEU Proton-translocating NAD(P)(+) transhydrogenase OS=Actinokineospora spheciospongiae OX=909613 GN=UO65_2894 PE=4 SV=1
MM1 pKa = 8.1VPMGIRR7 pKa = 11.84RR8 pKa = 11.84ARR10 pKa = 11.84GGRR13 pKa = 11.84TGPRR17 pKa = 11.84LPVRR21 pKa = 11.84ARR23 pKa = 11.84QRR25 pKa = 11.84TGARR29 pKa = 11.84SWRR32 pKa = 11.84TGWWSLDD39 pKa = 2.91GRR41 pKa = 11.84SRR43 pKa = 11.84ARR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84AMARR51 pKa = 11.84RR52 pKa = 11.84WLTMALRR59 pKa = 11.84RR60 pKa = 11.84SVAPSGRR67 pKa = 11.84DD68 pKa = 3.21TVRR71 pKa = 11.84LGRR74 pKa = 11.84ARR76 pKa = 11.84GAWALRR82 pKa = 11.84RR83 pKa = 11.84GTVAPHH89 pKa = 6.26RR90 pKa = 11.84PAVARR95 pKa = 11.84LRR97 pKa = 3.91

Molecular weight: 11.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6610

0

6610

2183027

37

9305

330.3

35.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.012 ± 0.046

0.75 ± 0.008

6.247 ± 0.023

5.323 ± 0.03

2.748 ± 0.017

9.637 ± 0.045

2.372 ± 0.019

2.746 ± 0.024

1.699 ± 0.022

10.487 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.507 ± 0.013

1.687 ± 0.016

6.333 ± 0.038

2.513 ± 0.023

8.265 ± 0.036

4.721 ± 0.024

6.298 ± 0.034

9.464 ± 0.035

1.47 ± 0.013

1.723 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski