Amino acid dipepetide frequency for Actinokineospora spheciospongiae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.833AlaAla: 22.833 ± 0.191	1.037AlaCys: 1.037 ± 0.027	9.313AlaAsp: 9.313 ± 0.09	9.453AlaGlu: 9.453 ± 0.083	3.524AlaPhe: 3.524 ± 0.047	13.672AlaGly: 13.672 ± 0.112	3.181AlaHis: 3.181 ± 0.042	3.487AlaIle: 3.487 ± 0.048	2.569AlaLys: 2.569 ± 0.047	15.213AlaLeu: 15.213 ± 0.129	2.24AlaMet: 2.24 ± 0.037	2.131AlaAsn: 2.131 ± 0.039	7.122AlaPro: 7.122 ± 0.085	3.497AlaGln: 3.497 ± 0.044	10.273AlaArg: 10.273 ± 0.093	4.955AlaSer: 4.955 ± 0.054	7.824AlaThr: 7.824 ± 0.077	13.769AlaVal: 13.769 ± 0.125	1.81AlaTrp: 1.81 ± 0.033	2.215AlaTyr: 2.215 ± 0.033	0.0AlaXaa: 0.0 ± 0.0
Cys	1.191CysAla: 1.191 ± 0.028	0.087CysCys: 0.087 ± 0.007	0.442CysAsp: 0.442 ± 0.013	0.332CysGlu: 0.332 ± 0.012	0.221CysPhe: 0.221 ± 0.011	0.9CysGly: 0.9 ± 0.021	0.187CysHis: 0.187 ± 0.009	0.115CysIle: 0.115 ± 0.008	0.098CysLys: 0.098 ± 0.007	0.72CysLeu: 0.72 ± 0.018	0.09CysMet: 0.09 ± 0.007	0.113CysAsn: 0.113 ± 0.007	0.498CysPro: 0.498 ± 0.015	0.149CysGln: 0.149 ± 0.009	0.568CysArg: 0.568 ± 0.018	0.418CysSer: 0.418 ± 0.016	0.45CysThr: 0.45 ± 0.016	0.655CysVal: 0.655 ± 0.018	0.12CysTrp: 0.12 ± 0.008	0.146CysTyr: 0.146 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.622AspAla: 7.622 ± 0.061	0.412AspCys: 0.412 ± 0.014	3.8AspAsp: 3.8 ± 0.054	3.655AspGlu: 3.655 ± 0.05	1.656AspPhe: 1.656 ± 0.03	6.218AspGly: 6.218 ± 0.064	1.618AspHis: 1.618 ± 0.028	1.599AspIle: 1.599 ± 0.028	0.984AspLys: 0.984 ± 0.026	7.448AspLeu: 7.448 ± 0.073	0.734AspMet: 0.734 ± 0.019	1.06AspAsn: 1.06 ± 0.027	5.277AspPro: 5.277 ± 0.056	1.545AspGln: 1.545 ± 0.03	5.728AspArg: 5.728 ± 0.058	2.553AspSer: 2.553 ± 0.034	3.362AspThr: 3.362 ± 0.039	5.126AspVal: 5.126 ± 0.045	0.956AspTrp: 0.956 ± 0.024	1.114AspTyr: 1.114 ± 0.022	0.0AspXaa: 0.0 ± 0.0
Glu	6.52GluAla: 6.52 ± 0.075	0.349GluCys: 0.349 ± 0.014	2.691GluAsp: 2.691 ± 0.039	2.63GluGlu: 2.63 ± 0.042	1.617GluPhe: 1.617 ± 0.028	3.597GluGly: 3.597 ± 0.041	1.811GluHis: 1.811 ± 0.03	1.737GluIle: 1.737 ± 0.032	1.004GluLys: 1.004 ± 0.024	6.662GluLeu: 6.662 ± 0.062	0.709GluMet: 0.709 ± 0.022	0.91GluAsn: 0.91 ± 0.022	3.427GluPro: 3.427 ± 0.05	2.252GluGln: 2.252 ± 0.036	5.208GluArg: 5.208 ± 0.064	2.391GluSer: 2.391 ± 0.039	2.478GluThr: 2.478 ± 0.032	5.53GluVal: 5.53 ± 0.059	0.737GluTrp: 0.737 ± 0.018	0.975GluTyr: 0.975 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	4.026PheAla: 4.026 ± 0.052	0.246PheCys: 0.246 ± 0.01	2.256PheAsp: 2.256 ± 0.039	1.322PheGlu: 1.322 ± 0.027	0.817PhePhe: 0.817 ± 0.021	3.175PheGly: 3.175 ± 0.047	0.64PheHis: 0.64 ± 0.017	0.638PheIle: 0.638 ± 0.02	0.393PheLys: 0.393 ± 0.014	2.593PheLeu: 2.593 ± 0.034	0.301PheMet: 0.301 ± 0.012	0.497PheAsn: 0.497 ± 0.017	1.479PhePro: 1.479 ± 0.026	0.598PheGln: 0.598 ± 0.019	1.842PheArg: 1.842 ± 0.03	1.411PheSer: 1.411 ± 0.028	2.168PheThr: 2.168 ± 0.037	2.208PheVal: 2.208 ± 0.034	0.377PheTrp: 0.377 ± 0.015	0.486PheTyr: 0.486 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.758GlyAla: 11.758 ± 0.103	0.827GlyCys: 0.827 ± 0.02	5.283GlyAsp: 5.283 ± 0.052	5.121GlyGlu: 5.121 ± 0.057	3.032GlyPhe: 3.032 ± 0.043	9.594GlyGly: 9.594 ± 0.141	2.427GlyHis: 2.427 ± 0.045	3.083GlyIle: 3.083 ± 0.044	2.146GlyLys: 2.146 ± 0.038	9.6GlyLeu: 9.6 ± 0.076	1.928GlyMet: 1.928 ± 0.034	1.737GlyAsn: 1.737 ± 0.042	5.237GlyPro: 5.237 ± 0.061	2.754GlyGln: 2.754 ± 0.059	7.579GlyArg: 7.579 ± 0.076	5.174GlySer: 5.174 ± 0.065	6.33GlyThr: 6.33 ± 0.073	8.951GlyVal: 8.951 ± 0.086	1.728GlyTrp: 1.728 ± 0.032	2.078GlyTyr: 2.078 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	2.749HisAla: 2.749 ± 0.039	0.206HisCys: 0.206 ± 0.01	1.429HisAsp: 1.429 ± 0.025	1.112HisGlu: 1.112 ± 0.024	0.599HisPhe: 0.599 ± 0.015	2.398HisGly: 2.398 ± 0.04	0.809HisHis: 0.809 ± 0.025	0.527HisIle: 0.527 ± 0.015	0.259HisLys: 0.259 ± 0.011	2.702HisLeu: 2.702 ± 0.042	0.282HisMet: 0.282 ± 0.013	0.4HisAsn: 0.4 ± 0.015	2.033HisPro: 2.033 ± 0.037	0.67HisGln: 0.67 ± 0.021	2.508HisArg: 2.508 ± 0.05	1.014HisSer: 1.014 ± 0.022	1.33HisThr: 1.33 ± 0.026	1.853HisVal: 1.853 ± 0.033	0.374HisTrp: 0.374 ± 0.014	0.461HisTyr: 0.461 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	4.478IleAla: 4.478 ± 0.051	0.214IleCys: 0.214 ± 0.01	1.983IleAsp: 1.983 ± 0.04	1.613IleGlu: 1.613 ± 0.034	0.543IlePhe: 0.543 ± 0.019	3.267IleGly: 3.267 ± 0.046	0.493IleHis: 0.493 ± 0.016	0.734IleIle: 0.734 ± 0.023	0.535IleLys: 0.535 ± 0.014	1.805IleLeu: 1.805 ± 0.032	0.335IleMet: 0.335 ± 0.013	0.609IleAsn: 0.609 ± 0.02	1.676IlePro: 1.676 ± 0.029	0.56IleGln: 0.56 ± 0.019	1.926IleArg: 1.926 ± 0.028	1.474IleSer: 1.474 ± 0.03	2.395IleThr: 2.395 ± 0.038	2.16IleVal: 2.16 ± 0.033	0.265IleTrp: 0.265 ± 0.01	0.396IleTyr: 0.396 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	2.291LysAla: 2.291 ± 0.043	0.089LysCys: 0.089 ± 0.007	0.86LysAsp: 0.86 ± 0.027	0.724LysGlu: 0.724 ± 0.023	0.427LysPhe: 0.427 ± 0.017	1.28LysGly: 1.28 ± 0.031	0.369LysHis: 0.369 ± 0.013	0.636LysIle: 0.636 ± 0.021	0.487LysLys: 0.487 ± 0.019	1.767LysLeu: 1.767 ± 0.032	0.293LysMet: 0.293 ± 0.013	0.341LysAsn: 0.341 ± 0.013	1.224LysPro: 1.224 ± 0.026	0.566LysGln: 0.566 ± 0.02	1.322LysArg: 1.322 ± 0.032	0.971LysSer: 0.971 ± 0.026	1.093LysThr: 1.093 ± 0.025	1.721LysVal: 1.721 ± 0.034	0.211LysTrp: 0.211 ± 0.011	0.314LysTyr: 0.314 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	16.046LeuAla: 16.046 ± 0.139	0.784LeuCys: 0.784 ± 0.021	7.455LeuAsp: 7.455 ± 0.071	4.136LeuGlu: 4.136 ± 0.053	2.713LeuPhe: 2.713 ± 0.047	9.85LeuGly: 9.85 ± 0.083	2.33LeuHis: 2.33 ± 0.034	2.742LeuIle: 2.742 ± 0.044	1.316LeuLys: 1.316 ± 0.029	11.36LeuLeu: 11.36 ± 0.107	1.263LeuMet: 1.263 ± 0.028	1.548LeuAsn: 1.548 ± 0.027	6.824LeuPro: 6.824 ± 0.07	1.721LeuGln: 1.721 ± 0.029	9.438LeuArg: 9.438 ± 0.089	5.341LeuSer: 5.341 ± 0.051	6.754LeuThr: 6.754 ± 0.058	10.525LeuVal: 10.525 ± 0.078	1.271LeuTrp: 1.271 ± 0.027	1.458LeuTyr: 1.458 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.0MetAla: 2.0 ± 0.035	0.101MetCys: 0.101 ± 0.007	0.747MetAsp: 0.747 ± 0.018	0.54MetGlu: 0.54 ± 0.02	0.435MetPhe: 0.435 ± 0.014	1.203MetGly: 1.203 ± 0.025	0.283MetHis: 0.283 ± 0.012	0.53MetIle: 0.53 ± 0.017	0.27MetLys: 0.27 ± 0.012	1.493MetLeu: 1.493 ± 0.026	0.221MetMet: 0.221 ± 0.011	0.322MetAsn: 0.322 ± 0.012	0.965MetPro: 0.965 ± 0.023	0.343MetGln: 0.343 ± 0.013	1.299MetArg: 1.299 ± 0.026	1.154MetSer: 1.154 ± 0.023	1.414MetThr: 1.414 ± 0.028	1.373MetVal: 1.373 ± 0.027	0.176MetTrp: 0.176 ± 0.009	0.204MetTyr: 0.204 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	2.136AsnAla: 2.136 ± 0.036	0.151AsnCys: 0.151 ± 0.009	0.859AsnAsp: 0.859 ± 0.024	0.662AsnGlu: 0.662 ± 0.02	0.423AsnPhe: 0.423 ± 0.015	1.733AsnGly: 1.733 ± 0.043	0.414AsnHis: 0.414 ± 0.013	0.514AsnIle: 0.514 ± 0.016	0.308AsnLys: 0.308 ± 0.011	1.729AsnLeu: 1.729 ± 0.03	0.246AsnMet: 0.246 ± 0.011	0.398AsnAsn: 0.398 ± 0.015	1.527AsnPro: 1.527 ± 0.034	0.485AsnGln: 0.485 ± 0.018	1.458AsnArg: 1.458 ± 0.027	0.845AsnSer: 0.845 ± 0.019	1.137AsnThr: 1.137 ± 0.026	1.215AsnVal: 1.215 ± 0.026	0.256AsnTrp: 0.256 ± 0.012	0.375AsnTyr: 0.375 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	9.094ProAla: 9.094 ± 0.105	0.321ProCys: 0.321 ± 0.015	4.876ProAsp: 4.876 ± 0.053	4.139ProGlu: 4.139 ± 0.049	1.587ProPhe: 1.587 ± 0.031	7.421ProGly: 7.421 ± 0.074	1.393ProHis: 1.393 ± 0.029	1.471ProIle: 1.471 ± 0.026	1.013ProLys: 1.013 ± 0.023	5.282ProLeu: 5.282 ± 0.062	0.953ProMet: 0.953 ± 0.021	1.047ProAsn: 1.047 ± 0.027	4.097ProPro: 4.097 ± 0.078	1.555ProGln: 1.555 ± 0.036	4.325ProArg: 4.325 ± 0.057	2.697ProSer: 2.697 ± 0.04	4.06ProThr: 4.06 ± 0.063	6.158ProVal: 6.158 ± 0.067	0.903ProTrp: 0.903 ± 0.024	0.942ProTyr: 0.942 ± 0.022	0.0ProXaa: 0.0 ± 0.0
Gln	3.445GlnAla: 3.445 ± 0.046	0.167GlnCys: 0.167 ± 0.008	1.226GlnAsp: 1.226 ± 0.026	1.099GlnGlu: 1.099 ± 0.028	0.654GlnPhe: 0.654 ± 0.019	1.982GlnGly: 1.982 ± 0.04	0.603GlnHis: 0.603 ± 0.023	0.785GlnIle: 0.785 ± 0.022	0.413GlnLys: 0.413 ± 0.016	2.586GlnLeu: 2.586 ± 0.046	0.353GlnMet: 0.353 ± 0.014	0.452GlnAsn: 0.452 ± 0.014	1.751GlnPro: 1.751 ± 0.041	1.085GlnGln: 1.085 ± 0.037	2.523GlnArg: 2.523 ± 0.048	1.137GlnSer: 1.137 ± 0.041	1.189GlnThr: 1.189 ± 0.028	2.751GlnVal: 2.751 ± 0.036	0.425GlnTrp: 0.425 ± 0.014	0.504GlnTyr: 0.504 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	11.007ArgAla: 11.007 ± 0.086	0.636ArgCys: 0.636 ± 0.018	4.502ArgAsp: 4.502 ± 0.052	4.519ArgGlu: 4.519 ± 0.049	2.639ArgPhe: 2.639 ± 0.038	6.682ArgGly: 6.682 ± 0.069	2.109ArgHis: 2.109 ± 0.041	2.486ArgIle: 2.486 ± 0.043	1.474ArgLys: 1.474 ± 0.026	8.834ArgLeu: 8.834 ± 0.083	1.738ArgMet: 1.738 ± 0.03	1.26ArgAsn: 1.26 ± 0.024	4.994ArgPro: 4.994 ± 0.06	2.148ArgGln: 2.148 ± 0.039	7.767ArgArg: 7.767 ± 0.1	3.999ArgSer: 3.999 ± 0.049	5.143ArgThr: 5.143 ± 0.05	7.589ArgVal: 7.589 ± 0.077	1.507ArgTrp: 1.507 ± 0.029	1.613ArgTyr: 1.613 ± 0.028	0.0ArgXaa: 0.0 ± 0.0
Ser	6.623SerAla: 6.623 ± 0.078	0.376SerCys: 0.376 ± 0.013	2.464SerAsp: 2.464 ± 0.037	1.951SerGlu: 1.951 ± 0.037	1.398SerPhe: 1.398 ± 0.027	5.656SerGly: 5.656 ± 0.054	0.836SerHis: 0.836 ± 0.02	1.362SerIle: 1.362 ± 0.029	0.784SerLys: 0.784 ± 0.025	4.432SerLeu: 4.432 ± 0.05	0.927SerMet: 0.927 ± 0.022	0.789SerAsn: 0.789 ± 0.025	3.035SerPro: 3.035 ± 0.041	1.019SerGln: 1.019 ± 0.022	3.595SerArg: 3.595 ± 0.043	2.469SerSer: 2.469 ± 0.047	3.568SerThr: 3.568 ± 0.055	4.141SerVal: 4.141 ± 0.043	0.849SerTrp: 0.849 ± 0.024	0.936SerTyr: 0.936 ± 0.024	0.0SerXaa: 0.0 ± 0.0
Thr	9.633ThrAla: 9.633 ± 0.084	0.459ThrCys: 0.459 ± 0.016	3.836ThrAsp: 3.836 ± 0.054	3.182ThrGlu: 3.182 ± 0.043	1.516ThrPhe: 1.516 ± 0.028	7.111ThrGly: 7.111 ± 0.068	1.261ThrHis: 1.261 ± 0.028	1.713ThrIle: 1.713 ± 0.031	0.969ThrLys: 0.969 ± 0.028	5.55ThrLeu: 5.55 ± 0.056	0.892ThrMet: 0.892 ± 0.021	0.945ThrAsn: 0.945 ± 0.024	4.616ThrPro: 4.616 ± 0.072	1.216ThrGln: 1.216 ± 0.034	4.465ThrArg: 4.465 ± 0.043	3.028ThrSer: 3.028 ± 0.04	4.82ThrThr: 4.82 ± 0.073	5.744ThrVal: 5.744 ± 0.056	0.977ThrTrp: 0.977 ± 0.021	1.044ThrTyr: 1.044 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	12.77ValAla: 12.77 ± 0.104	0.757ValCys: 0.757 ± 0.018	6.559ValAsp: 6.559 ± 0.063	5.558ValGlu: 5.558 ± 0.057	2.624ValPhe: 2.624 ± 0.039	7.94ValGly: 7.94 ± 0.081	2.209ValHis: 2.209 ± 0.037	2.586ValIle: 2.586 ± 0.038	1.436ValLys: 1.436 ± 0.032	11.218ValLeu: 11.218 ± 0.097	1.142ValMet: 1.142 ± 0.023	1.65ValAsn: 1.65 ± 0.027	5.721ValPro: 5.721 ± 0.063	1.928ValGln: 1.928 ± 0.03	7.704ValArg: 7.704 ± 0.061	4.334ValSer: 4.334 ± 0.056	5.445ValThr: 5.445 ± 0.05	10.59ValVal: 10.59 ± 0.089	1.121ValTrp: 1.121 ± 0.027	1.34ValTyr: 1.34 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.668TrpAla: 1.668 ± 0.029	0.142TrpCys: 0.142 ± 0.008	0.752TrpAsp: 0.752 ± 0.02	0.66TrpGlu: 0.66 ± 0.018	0.519TrpPhe: 0.519 ± 0.018	1.009TrpGly: 1.009 ± 0.026	0.352TrpHis: 0.352 ± 0.011	0.402TrpIle: 0.402 ± 0.015	0.279TrpLys: 0.279 ± 0.012	1.83TrpLeu: 1.83 ± 0.037	0.262TrpMet: 0.262 ± 0.011	0.34TrpAsn: 0.34 ± 0.014	0.797TrpPro: 0.797 ± 0.018	0.53TrpGln: 0.53 ± 0.016	1.376TrpArg: 1.376 ± 0.026	0.98TrpSer: 0.98 ± 0.021	0.975TrpThr: 0.975 ± 0.021	1.206TrpVal: 1.206 ± 0.028	0.35TrpTrp: 0.35 ± 0.016	0.266TrpTyr: 0.266 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.23TyrAla: 2.23 ± 0.035	0.14TyrCys: 0.14 ± 0.007	1.134TyrAsp: 1.134 ± 0.031	0.825TyrGlu: 0.825 ± 0.021	0.531TyrPhe: 0.531 ± 0.02	1.683TyrGly: 1.683 ± 0.03	0.392TyrHis: 0.392 ± 0.014	0.317TyrIle: 0.317 ± 0.013	0.251TyrLys: 0.251 ± 0.01	2.047TyrLeu: 2.047 ± 0.034	0.165TyrMet: 0.165 ± 0.008	0.322TyrAsn: 0.322 ± 0.012	1.033TyrPro: 1.033 ± 0.024	0.505TyrGln: 0.505 ± 0.018	1.743TyrArg: 1.743 ± 0.031	0.861TyrSer: 0.861 ± 0.023	1.041TyrThr: 1.041 ± 0.028	1.367TyrVal: 1.367 ± 0.028	0.281TyrTrp: 0.281 ± 0.013	0.363TyrTyr: 0.363 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6610 proteins (2183027 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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