Amino acid dipepetide frequency for Bacillus sp. AFS006103

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.756AlaAla: 5.756 ± 0.07
0.641AlaCys: 0.641 ± 0.024
3.284AlaAsp: 3.284 ± 0.05
4.474AlaGlu: 4.474 ± 0.06
3.108AlaPhe: 3.108 ± 0.046
5.267AlaGly: 5.267 ± 0.076
1.192AlaHis: 1.192 ± 0.029
5.816AlaIle: 5.816 ± 0.083
4.817AlaLys: 4.817 ± 0.068
6.867AlaLeu: 6.867 ± 0.077
1.927AlaMet: 1.927 ± 0.039
2.976AlaAsn: 2.976 ± 0.043
2.109AlaPro: 2.109 ± 0.044
2.037AlaGln: 2.037 ± 0.041
2.507AlaArg: 2.507 ± 0.044
3.95AlaSer: 3.95 ± 0.056
3.347AlaThr: 3.347 ± 0.059
5.12AlaVal: 5.12 ± 0.068
0.601AlaTrp: 0.601 ± 0.024
2.209AlaTyr: 2.209 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.459CysAla: 0.459 ± 0.017
0.116CysCys: 0.116 ± 0.01
0.382CysAsp: 0.382 ± 0.017
0.508CysGlu: 0.508 ± 0.016
0.341CysPhe: 0.341 ± 0.016
0.702CysGly: 0.702 ± 0.023
0.264CysHis: 0.264 ± 0.021
0.594CysIle: 0.594 ± 0.02
0.412CysLys: 0.412 ± 0.022
0.788CysLeu: 0.788 ± 0.024
0.208CysMet: 0.208 ± 0.012
0.322CysAsn: 0.322 ± 0.017
0.41CysPro: 0.41 ± 0.019
0.25CysGln: 0.25 ± 0.014
0.281CysArg: 0.281 ± 0.015
0.564CysSer: 0.564 ± 0.021
0.402CysThr: 0.402 ± 0.018
0.446CysVal: 0.446 ± 0.019
0.076CysTrp: 0.076 ± 0.007
0.286CysTyr: 0.286 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.09AspAla: 3.09 ± 0.05
0.395AspCys: 0.395 ± 0.016
2.189AspAsp: 2.189 ± 0.049
3.878AspGlu: 3.878 ± 0.057
2.497AspPhe: 2.497 ± 0.045
3.199AspGly: 3.199 ± 0.055
1.104AspHis: 1.104 ± 0.028
3.954AspIle: 3.954 ± 0.054
3.222AspLys: 3.222 ± 0.052
5.066AspLeu: 5.066 ± 0.068
1.193AspMet: 1.193 ± 0.027
1.833AspAsn: 1.833 ± 0.046
1.96AspPro: 1.96 ± 0.035
1.986AspGln: 1.986 ± 0.04
2.138AspArg: 2.138 ± 0.042
2.61AspSer: 2.61 ± 0.042
2.404AspThr: 2.404 ± 0.034
3.436AspVal: 3.436 ± 0.049
0.649AspTrp: 0.649 ± 0.024
2.1AspTyr: 2.1 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
4.924GluAla: 4.924 ± 0.067
0.407GluCys: 0.407 ± 0.02
3.423GluAsp: 3.423 ± 0.046
6.179GluGlu: 6.179 ± 0.096
2.71GluPhe: 2.71 ± 0.05
4.118GluGly: 4.118 ± 0.049
1.382GluHis: 1.382 ± 0.035
5.806GluIle: 5.806 ± 0.073
6.666GluLys: 6.666 ± 0.076
7.012GluLeu: 7.012 ± 0.084
2.287GluMet: 2.287 ± 0.038
3.732GluAsn: 3.732 ± 0.058
1.85GluPro: 1.85 ± 0.04
2.986GluGln: 2.986 ± 0.047
3.24GluArg: 3.24 ± 0.057
3.377GluSer: 3.377 ± 0.053
3.682GluThr: 3.682 ± 0.052
4.93GluVal: 4.93 ± 0.068
0.815GluTrp: 0.815 ± 0.027
2.307GluTyr: 2.307 ± 0.04
0.0GluXaa: 0.0 ± 0.0
Phe
3.117PheAla: 3.117 ± 0.046
0.412PheCys: 0.412 ± 0.017
2.355PheAsp: 2.355 ± 0.039
2.964PheGlu: 2.964 ± 0.051
2.461PhePhe: 2.461 ± 0.048
3.415PheGly: 3.415 ± 0.054
1.034PheHis: 1.034 ± 0.024
4.007PheIle: 4.007 ± 0.065
2.575PheLys: 2.575 ± 0.046
4.877PheLeu: 4.877 ± 0.067
1.184PheMet: 1.184 ± 0.03
2.054PheAsn: 2.054 ± 0.036
1.768PhePro: 1.768 ± 0.034
1.704PheGln: 1.704 ± 0.036
1.575PheArg: 1.575 ± 0.038
3.303PheSer: 3.303 ± 0.05
2.691PheThr: 2.691 ± 0.046
3.021PheVal: 3.021 ± 0.048
0.513PheTrp: 0.513 ± 0.02
1.804PheTyr: 1.804 ± 0.042
0.0PheXaa: 0.0 ± 0.0
Gly
4.73GlyAla: 4.73 ± 0.065
0.669GlyCys: 0.669 ± 0.024
3.059GlyAsp: 3.059 ± 0.053
4.298GlyGlu: 4.298 ± 0.048
3.546GlyPhe: 3.546 ± 0.067
4.983GlyGly: 4.983 ± 0.084
1.413GlyHis: 1.413 ± 0.033
6.055GlyIle: 6.055 ± 0.069
5.365GlyLys: 5.365 ± 0.061
6.733GlyLeu: 6.733 ± 0.078
2.09GlyMet: 2.09 ± 0.042
2.944GlyAsn: 2.944 ± 0.049
1.809GlyPro: 1.809 ± 0.04
2.216GlyGln: 2.216 ± 0.038
2.665GlyArg: 2.665 ± 0.043
3.965GlySer: 3.965 ± 0.054
4.15GlyThr: 4.15 ± 0.063
5.036GlyVal: 5.036 ± 0.059
0.841GlyTrp: 0.841 ± 0.025
2.835GlyTyr: 2.835 ± 0.052
0.001GlyXaa: 0.001 ± 0.001
His
1.149HisAla: 1.149 ± 0.028
0.198HisCys: 0.198 ± 0.013
0.984HisAsp: 0.984 ± 0.028
1.289HisGlu: 1.289 ± 0.031
1.087HisPhe: 1.087 ± 0.028
1.436HisGly: 1.436 ± 0.033
0.632HisHis: 0.632 ± 0.025
1.428HisIle: 1.428 ± 0.031
1.031HisLys: 1.031 ± 0.026
2.155HisLeu: 2.155 ± 0.037
0.48HisMet: 0.48 ± 0.017
0.772HisAsn: 0.772 ± 0.022
1.122HisPro: 1.122 ± 0.028
0.863HisGln: 0.863 ± 0.023
0.849HisArg: 0.849 ± 0.023
1.31HisSer: 1.31 ± 0.03
1.033HisThr: 1.033 ± 0.029
1.296HisVal: 1.296 ± 0.027
0.218HisTrp: 0.218 ± 0.012
0.848HisTyr: 0.848 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
5.962IleAla: 5.962 ± 0.082
0.706IleCys: 0.706 ± 0.022
4.277IleAsp: 4.277 ± 0.055
5.694IleGlu: 5.694 ± 0.073
3.549IlePhe: 3.549 ± 0.062
5.97IleGly: 5.97 ± 0.082
1.722IleHis: 1.722 ± 0.036
6.314IleIle: 6.314 ± 0.086
5.117IleLys: 5.117 ± 0.06
7.706IleLeu: 7.706 ± 0.092
1.822IleMet: 1.822 ± 0.037
3.794IleAsn: 3.794 ± 0.054
3.46IlePro: 3.46 ± 0.055
3.013IleGln: 3.013 ± 0.045
3.122IleArg: 3.122 ± 0.048
5.369IleSer: 5.369 ± 0.063
4.434IleThr: 4.434 ± 0.057
5.483IleVal: 5.483 ± 0.067
0.708IleTrp: 0.708 ± 0.024
2.503IleTyr: 2.503 ± 0.044
0.0IleXaa: 0.0 ± 0.0
Lys
4.826LysAla: 4.826 ± 0.056
0.392LysCys: 0.392 ± 0.018
3.93LysAsp: 3.93 ± 0.057
6.743LysGlu: 6.743 ± 0.078
2.173LysPhe: 2.173 ± 0.04
4.802LysGly: 4.802 ± 0.057
1.246LysHis: 1.246 ± 0.026
5.248LysIle: 5.248 ± 0.055
6.221LysLys: 6.221 ± 0.072
6.183LysLeu: 6.183 ± 0.068
2.318LysMet: 2.318 ± 0.043
3.815LysAsn: 3.815 ± 0.054
2.291LysPro: 2.291 ± 0.042
3.085LysGln: 3.085 ± 0.052
3.194LysArg: 3.194 ± 0.05
3.79LysSer: 3.79 ± 0.051
3.841LysThr: 3.841 ± 0.052
5.023LysVal: 5.023 ± 0.061
0.854LysTrp: 0.854 ± 0.027
2.294LysTyr: 2.294 ± 0.047
0.0LysXaa: 0.0 ± 0.0
Leu
7.185LeuAla: 7.185 ± 0.082
0.716LeuCys: 0.716 ± 0.024
4.741LeuAsp: 4.741 ± 0.066
6.713LeuGlu: 6.713 ± 0.085
4.987LeuPhe: 4.987 ± 0.073
6.592LeuGly: 6.592 ± 0.074
1.853LeuHis: 1.853 ± 0.035
7.692LeuIle: 7.692 ± 0.088
7.176LeuLys: 7.176 ± 0.079
10.237LeuLeu: 10.237 ± 0.112
2.537LeuMet: 2.537 ± 0.049
4.516LeuAsn: 4.516 ± 0.059
3.959LeuPro: 3.959 ± 0.06
3.416LeuGln: 3.416 ± 0.051
3.588LeuArg: 3.588 ± 0.053
6.727LeuSer: 6.727 ± 0.069
5.816LeuThr: 5.816 ± 0.061
6.368LeuVal: 6.368 ± 0.072
0.87LeuTrp: 0.87 ± 0.023
3.068LeuTyr: 3.068 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
1.974MetAla: 1.974 ± 0.043
0.157MetCys: 0.157 ± 0.01
1.44MetAsp: 1.44 ± 0.032
2.008MetGlu: 2.008 ± 0.04
1.066MetPhe: 1.066 ± 0.035
1.866MetGly: 1.866 ± 0.036
0.417MetHis: 0.417 ± 0.017
2.255MetIle: 2.255 ± 0.038
2.417MetLys: 2.417 ± 0.045
2.423MetLeu: 2.423 ± 0.043
0.801MetMet: 0.801 ± 0.025
1.568MetAsn: 1.568 ± 0.033
0.992MetPro: 0.992 ± 0.033
0.807MetGln: 0.807 ± 0.027
0.995MetArg: 0.995 ± 0.029
1.618MetSer: 1.618 ± 0.032
1.533MetThr: 1.533 ± 0.033
1.894MetVal: 1.894 ± 0.039
0.183MetTrp: 0.183 ± 0.012
0.725MetTyr: 0.725 ± 0.02
0.0MetXaa: 0.0 ± 0.0
Asn
2.701AsnAla: 2.701 ± 0.045
0.382AsnCys: 0.382 ± 0.017
2.274AsnAsp: 2.274 ± 0.042
3.504AsnGlu: 3.504 ± 0.053
1.863AsnPhe: 1.863 ± 0.037
3.522AsnGly: 3.522 ± 0.051
1.173AsnHis: 1.173 ± 0.032
3.544AsnIle: 3.544 ± 0.057
3.269AsnLys: 3.269 ± 0.052
4.281AsnLeu: 4.281 ± 0.057
1.17AsnMet: 1.17 ± 0.026
2.231AsnAsn: 2.231 ± 0.049
2.319AsnPro: 2.319 ± 0.044
2.241AsnGln: 2.241 ± 0.047
2.139AsnArg: 2.139 ± 0.039
2.667AsnSer: 2.667 ± 0.046
2.239AsnThr: 2.239 ± 0.046
3.03AsnVal: 3.03 ± 0.048
0.555AsnTrp: 0.555 ± 0.021
1.753AsnTyr: 1.753 ± 0.043
0.0AsnXaa: 0.0 ± 0.0
Pro
2.322ProAla: 2.322 ± 0.046
0.225ProCys: 0.225 ± 0.014
1.875ProAsp: 1.875 ± 0.035
2.871ProGlu: 2.871 ± 0.042
2.056ProPhe: 2.056 ± 0.042
2.362ProGly: 2.362 ± 0.042
0.742ProHis: 0.742 ± 0.019
3.05ProIle: 3.05 ± 0.048
2.435ProLys: 2.435 ± 0.044
3.517ProLeu: 3.517 ± 0.052
0.854ProMet: 0.854 ± 0.025
1.837ProAsn: 1.837 ± 0.038
0.969ProPro: 0.969 ± 0.033
1.147ProGln: 1.147 ± 0.03
1.157ProArg: 1.157 ± 0.029
2.305ProSer: 2.305 ± 0.049
1.986ProThr: 1.986 ± 0.04
2.877ProVal: 2.877 ± 0.048
0.389ProTrp: 0.389 ± 0.017
1.402ProTyr: 1.402 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
2.617GlnAla: 2.617 ± 0.046
0.218GlnCys: 0.218 ± 0.014
1.527GlnAsp: 1.527 ± 0.037
2.696GlnGlu: 2.696 ± 0.048
1.695GlnPhe: 1.695 ± 0.037
2.145GlnGly: 2.145 ± 0.04
0.71GlnHis: 0.71 ± 0.022
2.718GlnIle: 2.718 ± 0.044
2.838GlnLys: 2.838 ± 0.047
4.013GlnLeu: 4.013 ± 0.063
1.102GlnMet: 1.102 ± 0.034
1.678GlnAsn: 1.678 ± 0.036
1.23GlnPro: 1.23 ± 0.032
1.676GlnGln: 1.676 ± 0.042
1.423GlnArg: 1.423 ± 0.031
2.105GlnSer: 2.105 ± 0.04
1.959GlnThr: 1.959 ± 0.041
2.392GlnVal: 2.392 ± 0.048
0.42GlnTrp: 0.42 ± 0.018
1.344GlnTyr: 1.344 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
2.33ArgAla: 2.33 ± 0.037
0.271ArgCys: 0.271 ± 0.014
1.971ArgAsp: 1.971 ± 0.043
3.039ArgGlu: 3.039 ± 0.049
1.933ArgPhe: 1.933 ± 0.035
2.311ArgGly: 2.311 ± 0.044
0.716ArgHis: 0.716 ± 0.02
3.136ArgIle: 3.136 ± 0.046
3.202ArgLys: 3.202 ± 0.051
3.912ArgLeu: 3.912 ± 0.047
1.266ArgMet: 1.266 ± 0.036
2.014ArgAsn: 2.014 ± 0.041
1.33ArgPro: 1.33 ± 0.032
1.354ArgGln: 1.354 ± 0.035
1.733ArgArg: 1.733 ± 0.039
2.172ArgSer: 2.172 ± 0.042
2.003ArgThr: 2.003 ± 0.037
2.581ArgVal: 2.581 ± 0.042
0.419ArgTrp: 0.419 ± 0.015
1.533ArgTyr: 1.533 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
3.697SerAla: 3.697 ± 0.053
0.479SerCys: 0.479 ± 0.021
2.72SerAsp: 2.72 ± 0.043
3.93SerGlu: 3.93 ± 0.053
3.506SerPhe: 3.506 ± 0.056
4.538SerGly: 4.538 ± 0.054
1.202SerHis: 1.202 ± 0.03
5.131SerIle: 5.131 ± 0.06
4.098SerLys: 4.098 ± 0.052
6.255SerLeu: 6.255 ± 0.071
1.663SerMet: 1.663 ± 0.04
2.767SerAsn: 2.767 ± 0.054
2.153SerPro: 2.153 ± 0.034
2.033SerGln: 2.033 ± 0.042
2.28SerArg: 2.28 ± 0.041
3.941SerSer: 3.941 ± 0.072
3.189SerThr: 3.189 ± 0.051
3.981SerVal: 3.981 ± 0.05
0.627SerTrp: 0.627 ± 0.019
2.305SerTyr: 2.305 ± 0.042
0.001SerXaa: 0.001 ± 0.001
Thr
3.898ThrAla: 3.898 ± 0.067
0.402ThrCys: 0.402 ± 0.016
2.622ThrAsp: 2.622 ± 0.05
3.334ThrGlu: 3.334 ± 0.047
2.68ThrPhe: 2.68 ± 0.047
4.244ThrGly: 4.244 ± 0.056
1.02ThrHis: 1.02 ± 0.026
4.807ThrIle: 4.807 ± 0.063
3.529ThrLys: 3.529 ± 0.051
5.122ThrLeu: 5.122 ± 0.065
1.279ThrMet: 1.279 ± 0.031
2.661ThrAsn: 2.661 ± 0.052
2.225ThrPro: 2.225 ± 0.037
1.421ThrGln: 1.421 ± 0.034
1.867ThrArg: 1.867 ± 0.036
3.321ThrSer: 3.321 ± 0.051
3.083ThrThr: 3.083 ± 0.059
4.19ThrVal: 4.19 ± 0.069
0.548ThrTrp: 0.548 ± 0.024
1.882ThrTyr: 1.882 ± 0.043
0.0ThrXaa: 0.0 ± 0.0
Val
4.65ValAla: 4.65 ± 0.067
0.62ValCys: 0.62 ± 0.019
3.524ValAsp: 3.524 ± 0.054
4.678ValGlu: 4.678 ± 0.064
3.117ValPhe: 3.117 ± 0.046
4.704ValGly: 4.704 ± 0.061
1.283ValHis: 1.283 ± 0.032
5.781ValIle: 5.781 ± 0.066
4.897ValLys: 4.897 ± 0.059
6.662ValLeu: 6.662 ± 0.072
1.798ValMet: 1.798 ± 0.033
3.263ValAsn: 3.263 ± 0.046
2.707ValPro: 2.707 ± 0.047
2.255ValGln: 2.255 ± 0.04
2.494ValArg: 2.494 ± 0.045
4.432ValSer: 4.432 ± 0.058
4.121ValThr: 4.121 ± 0.067
4.875ValVal: 4.875 ± 0.075
0.626ValTrp: 0.626 ± 0.023
2.284ValTyr: 2.284 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
0.626TrpAla: 0.626 ± 0.023
0.076TrpCys: 0.076 ± 0.006
0.535TrpAsp: 0.535 ± 0.02
0.656TrpGlu: 0.656 ± 0.021
0.544TrpPhe: 0.544 ± 0.022
0.722TrpGly: 0.722 ± 0.025
0.207TrpHis: 0.207 ± 0.012
0.854TrpIle: 0.854 ± 0.025
0.81TrpLys: 0.81 ± 0.025
1.159TrpLeu: 1.159 ± 0.029
0.345TrpMet: 0.345 ± 0.016
0.594TrpAsn: 0.594 ± 0.022
0.247TrpPro: 0.247 ± 0.015
0.354TrpGln: 0.354 ± 0.018
0.395TrpArg: 0.395 ± 0.017
0.635TrpSer: 0.635 ± 0.019
0.469TrpThr: 0.469 ± 0.019
0.688TrpVal: 0.688 ± 0.024
0.143TrpTrp: 0.143 ± 0.009
0.387TrpTyr: 0.387 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.99TyrAla: 1.99 ± 0.039
0.329TyrCys: 0.329 ± 0.014
1.79TyrAsp: 1.79 ± 0.042
2.377TyrGlu: 2.377 ± 0.043
1.914TyrPhe: 1.914 ± 0.036
2.506TyrGly: 2.506 ± 0.041
0.868TyrHis: 0.868 ± 0.022
2.536TyrIle: 2.536 ± 0.046
2.171TyrLys: 2.171 ± 0.042
3.791TyrLeu: 3.791 ± 0.059
0.811TyrMet: 0.811 ± 0.026
1.485TyrAsn: 1.485 ± 0.039
1.467TyrPro: 1.467 ± 0.033
1.66TyrGln: 1.66 ± 0.037
1.583TyrArg: 1.583 ± 0.034
2.338TyrSer: 2.338 ± 0.048
1.754TyrThr: 1.754 ± 0.042
2.107TyrVal: 2.107 ± 0.047
0.392TyrTrp: 0.392 ± 0.015
1.464TyrTyr: 1.464 ± 0.043
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.001XaaGly: 0.001 ± 0.001
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.001XaaArg: 0.001 ± 0.001
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4840 proteins (1451607 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski