Brevibacterium phage Cantare

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 130 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3LZP4|A0A3G3LZP4_9CAUD Uncharacterized protein OS=Brevibacterium phage Cantare OX=2338395 GN=93 PE=4 SV=1
MM1 pKa = 7.21ATVTLTDD8 pKa = 3.41QNASLVVVGNSYY20 pKa = 10.73IFDD23 pKa = 4.4ADD25 pKa = 3.42DD26 pKa = 3.54RR27 pKa = 11.84AKK29 pKa = 11.1VEE31 pKa = 5.73DD32 pKa = 3.96GGEE35 pKa = 4.14LNGVVPVVVDD45 pKa = 3.37EE46 pKa = 6.06FYY48 pKa = 11.33FEE50 pKa = 5.25DD51 pKa = 4.42DD52 pKa = 3.58CAKK55 pKa = 10.16YY56 pKa = 10.27AVNVRR61 pKa = 11.84IEE63 pKa = 4.31GGDD66 pKa = 3.45FKK68 pKa = 11.11QWFGIEE74 pKa = 4.05DD75 pKa = 4.05TVTLVEE81 pKa = 4.42YY82 pKa = 10.89NN83 pKa = 3.32

Molecular weight: 9.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3LYR1|A0A3G3LYR1_9CAUD Uncharacterized protein OS=Brevibacterium phage Cantare OX=2338395 GN=60 PE=4 SV=1
MM1 pKa = 7.22FVGRR5 pKa = 11.84CSVTPKK11 pKa = 9.77YY12 pKa = 10.62SEE14 pKa = 4.0SRR16 pKa = 11.84VMLGTVLSEE25 pKa = 3.76SRR27 pKa = 11.84QVEE30 pKa = 4.12RR31 pKa = 11.84CLLRR35 pKa = 11.84LATNLVTNGYY45 pKa = 10.05GRR47 pKa = 11.84IRR49 pKa = 11.84KK50 pKa = 8.76LKK52 pKa = 8.83KK53 pKa = 9.95NHH55 pKa = 6.49VIDD58 pKa = 3.94PTQTLSDD65 pKa = 4.4RR66 pKa = 11.84MMEE69 pKa = 4.12WFGKK73 pKa = 9.42PIVIFAVWVLLFLLTLVYY91 pKa = 10.71SEE93 pKa = 4.69LSFRR97 pKa = 11.84ALMSAFIAWVGGLVTSRR114 pKa = 11.84LDD116 pKa = 3.17NALRR120 pKa = 11.84RR121 pKa = 11.84EE122 pKa = 4.32RR123 pKa = 11.84IYY125 pKa = 11.32KK126 pKa = 10.21DD127 pKa = 3.24VSDD130 pKa = 4.53KK131 pKa = 10.63IARR134 pKa = 11.84NRR136 pKa = 11.84KK137 pKa = 8.34FNRR140 pKa = 11.84NVYY143 pKa = 9.9

Molecular weight: 16.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

130

0

130

28420

35

1366

218.6

24.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.646 ± 0.257

0.676 ± 0.091

6.89 ± 0.278

6.833 ± 0.304

3.269 ± 0.142

7.474 ± 0.322

2.16 ± 0.143

6.133 ± 0.18

6.474 ± 0.27

7.062 ± 0.174

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.892 ± 0.112

4.627 ± 0.167

4.286 ± 0.144

3.1 ± 0.126

5.954 ± 0.243

6.165 ± 0.186

6.524 ± 0.221

7.09 ± 0.158

1.39 ± 0.091

3.357 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski