Amino acid dipepetide frequency for Brevibacterium phage Cantare

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.07AlaAla: 0.07 ± 0.061
0.633AlaCys: 0.633 ± 0.171
4.258AlaAsp: 4.258 ± 0.475
5.489AlaGlu: 5.489 ± 0.467
2.428AlaPhe: 2.428 ± 0.414
5.7AlaGly: 5.7 ± 0.592
1.654AlaHis: 1.654 ± 0.279
5.173AlaIle: 5.173 ± 0.489
6.193AlaLys: 6.193 ± 0.448
5.912AlaLeu: 5.912 ± 0.531
2.674AlaMet: 2.674 ± 0.298
4.258AlaAsn: 4.258 ± 0.528
3.308AlaPro: 3.308 ± 0.341
2.921AlaGln: 2.921 ± 0.353
4.961AlaArg: 4.961 ± 0.434
6.158AlaSer: 6.158 ± 0.576
5.841AlaThr: 5.841 ± 0.701
5.665AlaVal: 5.665 ± 0.508
0.704AlaTrp: 0.704 ± 0.151
2.463AlaTyr: 2.463 ± 0.294
0.0AlaXaa: 0.0 ± 0.0
Cys
0.457CysAla: 0.457 ± 0.129
0.0CysCys: 0.0 ± 0.0
0.457CysAsp: 0.457 ± 0.145
0.352CysGlu: 0.352 ± 0.119
0.141CysPhe: 0.141 ± 0.068
0.387CysGly: 0.387 ± 0.127
0.422CysHis: 0.422 ± 0.137
0.422CysIle: 0.422 ± 0.126
0.422CysLys: 0.422 ± 0.126
0.457CysLeu: 0.457 ± 0.129
0.07CysMet: 0.07 ± 0.054
0.457CysAsn: 0.457 ± 0.129
0.387CysPro: 0.387 ± 0.14
0.282CysGln: 0.282 ± 0.118
0.387CysArg: 0.387 ± 0.1
0.282CysSer: 0.282 ± 0.126
0.528CysThr: 0.528 ± 0.146
0.457CysVal: 0.457 ± 0.13
0.176CysTrp: 0.176 ± 0.102
0.211CysTyr: 0.211 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
5.7AspAla: 5.7 ± 0.491
0.493AspCys: 0.493 ± 0.143
5.876AspAsp: 5.876 ± 0.91
5.349AspGlu: 5.349 ± 0.569
2.217AspPhe: 2.217 ± 0.299
6.123AspGly: 6.123 ± 0.499
1.232AspHis: 1.232 ± 0.214
4.082AspIle: 4.082 ± 0.374
3.66AspLys: 3.66 ± 0.426
4.68AspLeu: 4.68 ± 0.405
1.865AspMet: 1.865 ± 0.358
2.674AspAsn: 2.674 ± 0.344
3.589AspPro: 3.589 ± 0.406
2.146AspGln: 2.146 ± 0.306
3.167AspArg: 3.167 ± 0.336
3.695AspSer: 3.695 ± 0.407
3.695AspThr: 3.695 ± 0.418
5.419AspVal: 5.419 ± 0.424
0.845AspTrp: 0.845 ± 0.145
2.393AspTyr: 2.393 ± 0.327
0.0AspXaa: 0.0 ± 0.0
Glu
5.665GluAla: 5.665 ± 0.447
0.493GluCys: 0.493 ± 0.181
5.595GluAsp: 5.595 ± 0.678
5.137GluGlu: 5.137 ± 0.544
2.569GluPhe: 2.569 ± 0.304
4.786GluGly: 4.786 ± 0.369
1.267GluHis: 1.267 ± 0.229
4.398GluIle: 4.398 ± 0.447
3.308GluLys: 3.308 ± 0.458
6.65GluLeu: 6.65 ± 0.599
1.865GluMet: 1.865 ± 0.298
2.393GluAsn: 2.393 ± 0.307
2.709GluPro: 2.709 ± 0.329
2.463GluGln: 2.463 ± 0.298
4.328GluArg: 4.328 ± 0.391
4.082GluSer: 4.082 ± 0.353
3.448GluThr: 3.448 ± 0.364
3.519GluVal: 3.519 ± 0.392
1.196GluTrp: 1.196 ± 0.186
2.463GluTyr: 2.463 ± 0.316
0.0GluXaa: 0.0 ± 0.0
Phe
2.534PheAla: 2.534 ± 0.262
0.07PheCys: 0.07 ± 0.05
2.639PheAsp: 2.639 ± 0.283
2.463PheGlu: 2.463 ± 0.247
0.985PhePhe: 0.985 ± 0.172
2.885PheGly: 2.885 ± 0.338
0.88PheHis: 0.88 ± 0.204
1.935PheIle: 1.935 ± 0.276
1.971PheLys: 1.971 ± 0.284
2.322PheLeu: 2.322 ± 0.362
0.845PheMet: 0.845 ± 0.142
1.161PheAsn: 1.161 ± 0.173
1.232PhePro: 1.232 ± 0.223
0.88PheGln: 0.88 ± 0.158
1.583PheArg: 1.583 ± 0.292
1.865PheSer: 1.865 ± 0.32
2.534PheThr: 2.534 ± 0.295
2.674PheVal: 2.674 ± 0.275
0.317PheTrp: 0.317 ± 0.095
0.915PheTyr: 0.915 ± 0.185
0.0PheXaa: 0.0 ± 0.0
Gly
6.263GlyAla: 6.263 ± 0.618
0.598GlyCys: 0.598 ± 0.176
4.75GlyAsp: 4.75 ± 0.465
4.223GlyGlu: 4.223 ± 0.418
2.322GlyPhe: 2.322 ± 0.29
6.087GlyGly: 6.087 ± 0.987
1.795GlyHis: 1.795 ± 0.308
5.841GlyIle: 5.841 ± 0.515
5.7GlyLys: 5.7 ± 0.574
5.349GlyLeu: 5.349 ± 0.443
2.322GlyMet: 2.322 ± 0.248
3.765GlyAsn: 3.765 ± 0.361
0.457GlyPro: 0.457 ± 0.172
2.956GlyGln: 2.956 ± 0.336
4.082GlyArg: 4.082 ± 0.441
4.082GlySer: 4.082 ± 0.47
5.56GlyThr: 5.56 ± 0.524
4.926GlyVal: 4.926 ± 0.476
0.95GlyTrp: 0.95 ± 0.188
2.709GlyTyr: 2.709 ± 0.335
0.0GlyXaa: 0.0 ± 0.0
His
1.83HisAla: 1.83 ± 0.242
0.352HisCys: 0.352 ± 0.096
1.267HisAsp: 1.267 ± 0.21
1.443HisGlu: 1.443 ± 0.242
0.669HisPhe: 0.669 ± 0.154
1.196HisGly: 1.196 ± 0.221
0.633HisHis: 0.633 ± 0.136
1.583HisIle: 1.583 ± 0.221
1.126HisLys: 1.126 ± 0.197
1.619HisLeu: 1.619 ± 0.247
0.704HisMet: 0.704 ± 0.151
1.126HisAsn: 1.126 ± 0.199
0.95HisPro: 0.95 ± 0.185
0.493HisGln: 0.493 ± 0.144
1.372HisArg: 1.372 ± 0.278
1.091HisSer: 1.091 ± 0.209
1.408HisThr: 1.408 ± 0.229
1.795HisVal: 1.795 ± 0.25
0.176HisTrp: 0.176 ± 0.084
0.774HisTyr: 0.774 ± 0.213
0.0HisXaa: 0.0 ± 0.0
Ile
5.032IleAla: 5.032 ± 0.365
0.211IleCys: 0.211 ± 0.085
5.524IleAsp: 5.524 ± 0.543
4.961IleGlu: 4.961 ± 0.442
2.041IlePhe: 2.041 ± 0.288
4.187IleGly: 4.187 ± 0.552
1.161IleHis: 1.161 ± 0.196
2.921IleIle: 2.921 ± 0.323
3.378IleLys: 3.378 ± 0.39
3.73IleLeu: 3.73 ± 0.326
1.408IleMet: 1.408 ± 0.183
3.519IleAsn: 3.519 ± 0.39
3.589IlePro: 3.589 ± 0.316
1.9IleGln: 1.9 ± 0.262
3.378IleArg: 3.378 ± 0.383
3.624IleSer: 3.624 ± 0.347
4.258IleThr: 4.258 ± 0.436
4.047IleVal: 4.047 ± 0.368
0.704IleTrp: 0.704 ± 0.14
1.759IleTyr: 1.759 ± 0.264
0.0IleXaa: 0.0 ± 0.0
Lys
6.123LysAla: 6.123 ± 0.696
0.352LysCys: 0.352 ± 0.104
4.223LysAsp: 4.223 ± 0.425
3.343LysGlu: 3.343 ± 0.395
2.358LysPhe: 2.358 ± 0.271
4.082LysGly: 4.082 ± 0.529
1.302LysHis: 1.302 ± 0.174
3.343LysIle: 3.343 ± 0.312
5.454LysLys: 5.454 ± 0.503
4.258LysLeu: 4.258 ± 0.397
1.935LysMet: 1.935 ± 0.282
3.061LysAsn: 3.061 ± 0.309
3.484LysPro: 3.484 ± 0.536
1.337LysGln: 1.337 ± 0.223
4.187LysArg: 4.187 ± 0.529
4.504LysSer: 4.504 ± 0.434
4.293LysThr: 4.293 ± 0.404
3.835LysVal: 3.835 ± 0.395
1.161LysTrp: 1.161 ± 0.184
2.111LysTyr: 2.111 ± 0.27
0.0LysXaa: 0.0 ± 0.0
Leu
5.841LeuAla: 5.841 ± 0.505
0.457LeuCys: 0.457 ± 0.14
4.645LeuAsp: 4.645 ± 0.52
4.398LeuGlu: 4.398 ± 0.361
1.654LeuPhe: 1.654 ± 0.266
5.067LeuGly: 5.067 ± 0.619
1.654LeuHis: 1.654 ± 0.237
3.554LeuIle: 3.554 ± 0.357
4.258LeuLys: 4.258 ± 0.525
4.258LeuLeu: 4.258 ± 0.383
2.85LeuMet: 2.85 ± 0.267
3.237LeuAsn: 3.237 ± 0.357
3.378LeuPro: 3.378 ± 0.321
2.428LeuGln: 2.428 ± 0.299
4.574LeuArg: 4.574 ± 0.401
5.349LeuSer: 5.349 ± 0.516
4.61LeuThr: 4.61 ± 0.361
4.997LeuVal: 4.997 ± 0.366
1.126LeuTrp: 1.126 ± 0.222
2.287LeuTyr: 2.287 ± 0.386
0.0LeuXaa: 0.0 ± 0.0
Met
2.885MetAla: 2.885 ± 0.293
0.176MetCys: 0.176 ± 0.073
1.971MetAsp: 1.971 ± 0.256
2.252MetGlu: 2.252 ± 0.302
1.196MetPhe: 1.196 ± 0.194
1.759MetGly: 1.759 ± 0.23
0.493MetHis: 0.493 ± 0.112
1.654MetIle: 1.654 ± 0.21
2.041MetLys: 2.041 ± 0.292
1.267MetLeu: 1.267 ± 0.199
0.704MetMet: 0.704 ± 0.143
1.372MetAsn: 1.372 ± 0.168
1.408MetPro: 1.408 ± 0.222
0.809MetGln: 0.809 ± 0.189
1.724MetArg: 1.724 ± 0.237
2.287MetSer: 2.287 ± 0.251
2.428MetThr: 2.428 ± 0.346
1.232MetVal: 1.232 ± 0.205
0.528MetTrp: 0.528 ± 0.13
0.739MetTyr: 0.739 ± 0.16
0.0MetXaa: 0.0 ± 0.0
Asn
3.765AsnAla: 3.765 ± 0.477
0.282AsnCys: 0.282 ± 0.116
2.709AsnAsp: 2.709 ± 0.276
3.554AsnGlu: 3.554 ± 0.378
1.232AsnPhe: 1.232 ± 0.213
4.504AsnGly: 4.504 ± 0.388
1.513AsnHis: 1.513 ± 0.238
2.639AsnIle: 2.639 ± 0.306
2.393AsnLys: 2.393 ± 0.36
2.956AsnLeu: 2.956 ± 0.354
1.196AsnMet: 1.196 ± 0.226
2.217AsnAsn: 2.217 ± 0.313
2.217AsnPro: 2.217 ± 0.326
2.111AsnGln: 2.111 ± 0.249
2.78AsnArg: 2.78 ± 0.336
2.322AsnSer: 2.322 ± 0.321
2.498AsnThr: 2.498 ± 0.284
3.343AsnVal: 3.343 ± 0.399
0.845AsnTrp: 0.845 ± 0.191
1.196AsnTyr: 1.196 ± 0.186
0.0AsnXaa: 0.0 ± 0.0
Pro
3.589ProAla: 3.589 ± 0.369
0.07ProCys: 0.07 ± 0.049
3.026ProAsp: 3.026 ± 0.412
3.237ProGlu: 3.237 ± 0.338
1.02ProPhe: 1.02 ± 0.202
4.293ProGly: 4.293 ± 0.418
0.95ProHis: 0.95 ± 0.155
1.83ProIle: 1.83 ± 0.26
3.554ProLys: 3.554 ± 0.438
2.393ProLeu: 2.393 ± 0.326
1.267ProMet: 1.267 ± 0.259
2.252ProAsn: 2.252 ± 0.273
1.619ProPro: 1.619 ± 0.255
0.88ProGln: 0.88 ± 0.183
1.83ProArg: 1.83 ± 0.309
2.956ProSer: 2.956 ± 0.36
2.991ProThr: 2.991 ± 0.311
3.66ProVal: 3.66 ± 0.356
0.563ProTrp: 0.563 ± 0.137
0.88ProTyr: 0.88 ± 0.21
0.0ProXaa: 0.0 ± 0.0
Gln
2.287GlnAla: 2.287 ± 0.287
0.352GlnCys: 0.352 ± 0.143
1.408GlnAsp: 1.408 ± 0.272
1.865GlnGlu: 1.865 ± 0.287
1.302GlnPhe: 1.302 ± 0.272
1.935GlnGly: 1.935 ± 0.285
0.669GlnHis: 0.669 ± 0.129
2.252GlnIle: 2.252 ± 0.268
2.252GlnLys: 2.252 ± 0.342
2.639GlnLeu: 2.639 ± 0.308
0.739GlnMet: 0.739 ± 0.174
1.232GlnAsn: 1.232 ± 0.207
1.02GlnPro: 1.02 ± 0.198
0.985GlnGln: 0.985 ± 0.251
1.971GlnArg: 1.971 ± 0.272
1.372GlnSer: 1.372 ± 0.228
2.006GlnThr: 2.006 ± 0.249
2.569GlnVal: 2.569 ± 0.306
0.739GlnTrp: 0.739 ± 0.156
1.408GlnTyr: 1.408 ± 0.213
0.0GlnXaa: 0.0 ± 0.0
Arg
5.278ArgAla: 5.278 ± 0.43
0.704ArgCys: 0.704 ± 0.183
3.66ArgAsp: 3.66 ± 0.424
3.589ArgGlu: 3.589 ± 0.422
2.076ArgPhe: 2.076 ± 0.24
3.061ArgGly: 3.061 ± 0.335
1.408ArgHis: 1.408 ± 0.226
3.941ArgIle: 3.941 ± 0.342
4.574ArgLys: 4.574 ± 0.431
4.363ArgLeu: 4.363 ± 0.39
1.971ArgMet: 1.971 ± 0.284
2.604ArgAsn: 2.604 ± 0.383
2.252ArgPro: 2.252 ± 0.31
1.759ArgGln: 1.759 ± 0.23
5.173ArgArg: 5.173 ± 0.683
2.956ArgSer: 2.956 ± 0.319
3.519ArgThr: 3.519 ± 0.392
3.976ArgVal: 3.976 ± 0.46
0.528ArgTrp: 0.528 ± 0.112
2.146ArgTyr: 2.146 ± 0.332
0.0ArgXaa: 0.0 ± 0.0
Ser
5.137SerAla: 5.137 ± 0.5
0.246SerCys: 0.246 ± 0.097
4.082SerAsp: 4.082 ± 0.444
3.765SerGlu: 3.765 ± 0.401
2.111SerPhe: 2.111 ± 0.275
4.75SerGly: 4.75 ± 0.507
1.232SerHis: 1.232 ± 0.184
4.363SerIle: 4.363 ± 0.326
3.871SerLys: 3.871 ± 0.408
4.821SerLeu: 4.821 ± 0.398
1.83SerMet: 1.83 ± 0.264
2.921SerAsn: 2.921 ± 0.276
2.358SerPro: 2.358 ± 0.312
1.689SerGln: 1.689 ± 0.213
3.237SerArg: 3.237 ± 0.376
3.519SerSer: 3.519 ± 0.419
3.835SerThr: 3.835 ± 0.352
4.328SerVal: 4.328 ± 0.394
0.774SerTrp: 0.774 ± 0.155
2.78SerTyr: 2.78 ± 0.288
0.0SerXaa: 0.0 ± 0.0
Thr
5.137ThrAla: 5.137 ± 0.475
0.317ThrCys: 0.317 ± 0.114
3.976ThrAsp: 3.976 ± 0.401
4.223ThrGlu: 4.223 ± 0.42
2.358ThrPhe: 2.358 ± 0.277
5.489ThrGly: 5.489 ± 0.553
1.161ThrHis: 1.161 ± 0.195
4.187ThrIle: 4.187 ± 0.437
4.398ThrLys: 4.398 ± 0.393
4.117ThrLeu: 4.117 ± 0.384
1.654ThrMet: 1.654 ± 0.238
3.272ThrAsn: 3.272 ± 0.335
3.8ThrPro: 3.8 ± 0.419
1.619ThrGln: 1.619 ± 0.213
3.484ThrArg: 3.484 ± 0.392
3.413ThrSer: 3.413 ± 0.425
4.504ThrThr: 4.504 ± 0.432
4.61ThrVal: 4.61 ± 0.422
0.845ThrTrp: 0.845 ± 0.173
2.674ThrTyr: 2.674 ± 0.305
0.0ThrXaa: 0.0 ± 0.0
Val
5.595ValAla: 5.595 ± 0.518
0.493ValCys: 0.493 ± 0.121
5.137ValAsp: 5.137 ± 0.491
4.997ValGlu: 4.997 ± 0.484
2.076ValPhe: 2.076 ± 0.277
4.504ValGly: 4.504 ± 0.462
1.337ValHis: 1.337 ± 0.211
4.786ValIle: 4.786 ± 0.398
3.765ValLys: 3.765 ± 0.415
5.067ValLeu: 5.067 ± 0.454
1.9ValMet: 1.9 ± 0.253
2.956ValAsn: 2.956 ± 0.331
3.132ValPro: 3.132 ± 0.345
2.111ValGln: 2.111 ± 0.254
4.293ValArg: 4.293 ± 0.446
4.821ValSer: 4.821 ± 0.368
4.504ValThr: 4.504 ± 0.486
5.7ValVal: 5.7 ± 0.587
1.056ValTrp: 1.056 ± 0.209
2.674ValTyr: 2.674 ± 0.335
0.0ValXaa: 0.0 ± 0.0
Trp
1.196TrpAla: 1.196 ± 0.215
0.211TrpCys: 0.211 ± 0.092
1.232TrpAsp: 1.232 ± 0.17
1.091TrpGlu: 1.091 ± 0.211
0.669TrpPhe: 0.669 ± 0.205
0.95TrpGly: 0.95 ± 0.201
0.317TrpHis: 0.317 ± 0.112
0.774TrpIle: 0.774 ± 0.166
0.95TrpLys: 0.95 ± 0.206
0.985TrpLeu: 0.985 ± 0.203
0.317TrpMet: 0.317 ± 0.12
0.633TrpAsn: 0.633 ± 0.149
0.282TrpPro: 0.282 ± 0.111
0.352TrpGln: 0.352 ± 0.113
0.739TrpArg: 0.739 ± 0.206
0.915TrpSer: 0.915 ± 0.201
0.633TrpThr: 0.633 ± 0.144
1.337TrpVal: 1.337 ± 0.201
0.141TrpTrp: 0.141 ± 0.066
0.176TrpTyr: 0.176 ± 0.082
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.076TyrAla: 2.076 ± 0.237
0.246TyrCys: 0.246 ± 0.097
2.463TyrAsp: 2.463 ± 0.292
2.604TyrGlu: 2.604 ± 0.316
1.267TyrPhe: 1.267 ± 0.202
2.991TyrGly: 2.991 ± 0.302
0.528TyrHis: 0.528 ± 0.145
1.654TyrIle: 1.654 ± 0.255
1.478TyrLys: 1.478 ± 0.223
2.78TyrLeu: 2.78 ± 0.423
0.774TyrMet: 0.774 ± 0.158
1.161TyrAsn: 1.161 ± 0.269
1.689TyrPro: 1.689 ± 0.237
0.88TyrGln: 0.88 ± 0.191
2.287TyrArg: 2.287 ± 0.342
2.358TyrSer: 2.358 ± 0.288
2.146TyrThr: 2.146 ± 0.273
2.815TyrVal: 2.815 ± 0.413
0.528TyrTrp: 0.528 ± 0.134
0.809TyrTyr: 0.809 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 130 proteins (28420 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski