Domestic cat hepadnavirus
Average proteome isoelectric point is 8.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U8SZQ3|A0A2U8SZQ3_9HEPA External core antigen OS=Domestic cat hepadnavirus OX=2107574 GN=C PE=3 SV=1
MM1 pKa = 7.64 GANQSIPNPLGFFPRR16 pKa = 11.84 HH17 pKa = 5.71 NLDD20 pKa = 3.23 LTDD23 pKa = 4.0 PAGTLGDD30 pKa = 4.14 FDD32 pKa = 5.88 HH33 pKa = 7.12 NPLKK37 pKa = 10.88 DD38 pKa = 3.41 PWPQSSHH45 pKa = 5.01 QSRR48 pKa = 11.84 GAWGPGFVPPHH59 pKa = 6.31 GGLLGSLSFATQGVVSAQPITNPPPRR85 pKa = 11.84 KK86 pKa = 9.28 KK87 pKa = 10.85 GRR89 pKa = 11.84 GPTPLTPPVRR99 pKa = 11.84 VTHH102 pKa = 6.24 PQMSPSSWRR111 pKa = 11.84 QKK113 pKa = 10.61 YY114 pKa = 10.42 GYY116 pKa = 10.42 LLLPKK121 pKa = 8.28 TTAAPGPTSPPQTTVAPTASSTSSPPLTIGAPAARR156 pKa = 11.84 MGNITSGPFGFLLGLQVGSFLWTKK180 pKa = 9.9 IQTIGQSADD189 pKa = 3.32 SWWTSLSFPGAIPGCIGQDD208 pKa = 3.2 SQYY211 pKa = 9.22 QTSKK215 pKa = 10.67 HH216 pKa = 6.39 SPTSCPPTCTGFPWMCLRR234 pKa = 11.84 RR235 pKa = 11.84 FIIYY239 pKa = 10.6 LLLLALLLIFSLVLLDD255 pKa = 3.78 WKK257 pKa = 11.0 GLLPVCPFPPGDD269 pKa = 3.82 SQTGNIRR276 pKa = 11.84 CKK278 pKa = 10.64 ACIGSAPEE286 pKa = 4.33 TPWPPLCCCTVPSDD300 pKa = 4.59 GNCTCWPIPSSWALGSYY317 pKa = 9.68 LWEE320 pKa = 4.06 LALARR325 pKa = 11.84 FSWLSSLVVWLQWLGGISPIVWCLLIWMTWFWGLRR360 pKa = 11.84 VWNILSQFMPLLFLLFYY377 pKa = 10.7 HH378 pKa = 7.07 LVYY381 pKa = 10.34 TT382 pKa = 4.99
Molecular weight: 41.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.237
IPC2_protein 8.024
IPC_protein 7.834
Toseland 7.322
ProMoST 8.404
Dawson 8.521
Bjellqvist 9.004
Wikipedia 8.419
Rodwell 8.536
Grimsley 7.293
Solomon 8.595
Lehninger 8.609
Nozaki 9.297
DTASelect 8.639
Thurlkill 8.697
EMBOSS 8.726
Sillero 9.063
Patrickios 3.465
IPC_peptide 8.595
IPC2_peptide 8.448
IPC2.peptide.svr19 8.539
Protein with the highest isoelectric point:
>tr|A0A2U8T025|A0A2U8T025_9HEPA Protein X OS=Domestic cat hepadnavirus OX=2107574 GN=X PE=3 SV=1
MM1 pKa = 7.47 HH2 pKa = 7.68 LFHH5 pKa = 7.08 LCVLFCSIPTVQASKK20 pKa = 11.21 LCLRR24 pKa = 11.84 WLWGMDD30 pKa = 2.7 IDD32 pKa = 4.44 PYY34 pKa = 11.38 KK35 pKa = 10.94 EE36 pKa = 4.02 FGTTSQLISFLPSDD50 pKa = 4.81 FFPALNDD57 pKa = 3.91 LVDD60 pKa = 4.86 TIQALYY66 pKa = 10.59 EE67 pKa = 4.01 EE68 pKa = 5.04 EE69 pKa = 4.11 LTGRR73 pKa = 11.84 EE74 pKa = 4.29 HH75 pKa = 7.77 CSPHH79 pKa = 5.03 HH80 pKa = 5.16 TALRR84 pKa = 11.84 VLLNCWEE91 pKa = 4.24 EE92 pKa = 4.25 SARR95 pKa = 11.84 MATWVRR101 pKa = 11.84 ANVEE105 pKa = 4.1 GAPLQDD111 pKa = 4.8 AIVAYY116 pKa = 10.69 VNSTVSLKK124 pKa = 10.44 LRR126 pKa = 11.84 QQMWFHH132 pKa = 6.56 LSCLTFGQHH141 pKa = 5.14 TVLEE145 pKa = 4.45 FLVSFGTWIRR155 pKa = 11.84 TPAPYY160 pKa = 9.94 RR161 pKa = 11.84 PPNAPILSTLPEE173 pKa = 4.04 HH174 pKa = 5.47 TVIRR178 pKa = 11.84 ARR180 pKa = 11.84 GAARR184 pKa = 11.84 RR185 pKa = 11.84 PARR188 pKa = 11.84 SPRR191 pKa = 11.84 RR192 pKa = 11.84 RR193 pKa = 11.84 TPSPRR198 pKa = 11.84 RR199 pKa = 11.84 RR200 pKa = 11.84 RR201 pKa = 11.84 SQSPRR206 pKa = 11.84 RR207 pKa = 11.84 RR208 pKa = 11.84 RR209 pKa = 11.84 SQSPTQSNCC218 pKa = 2.89
Molecular weight: 25.05 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.222
IPC2_protein 9.165
IPC_protein 9.853
Toseland 9.794
ProMoST 9.75
Dawson 10.087
Bjellqvist 9.984
Wikipedia 10.335
Rodwell 10.101
Grimsley 10.175
Solomon 10.189
Lehninger 10.145
Nozaki 10.116
DTASelect 9.897
Thurlkill 9.955
EMBOSS 10.248
Sillero 10.101
Patrickios 7.585
IPC_peptide 10.175
IPC2_peptide 9.575
IPC2.peptide.svr19 8.079
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1583
145
838
395.8
44.28
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.822 ± 0.456
2.78 ± 0.551
3.032 ± 0.165
2.716 ± 0.665
4.991 ± 0.573
6.886 ± 0.791
3.538 ± 0.75
3.095 ± 0.532
2.59 ± 0.57
12.697 ± 0.538
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.832 ± 0.078
2.527 ± 0.4
8.97 ± 1.11
3.79 ± 0.33
7.202 ± 1.23
9.16 ± 0.419
6.191 ± 1.025
5.433 ± 0.525
3.095 ± 0.697
2.653 ± 0.672
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here