Stenotrophomonas phage Ponderosa

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; unclassified Autographiviridae

Average proteome isoelectric point is 6.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NA72|A0A5B9NA72_9CAUD Uncharacterized protein OS=Stenotrophomonas phage Ponderosa OX=2591103 GN=CPT_Ponderosa_018 PE=4 SV=1
MM1 pKa = 7.67SDD3 pKa = 3.91DD4 pKa = 4.48APASTVSVQDD14 pKa = 3.41VEE16 pKa = 6.27LIAEE20 pKa = 4.77WFRR23 pKa = 11.84TCCAWCYY30 pKa = 9.7EE31 pKa = 3.97RR32 pKa = 11.84SIEE35 pKa = 5.04LSTDD39 pKa = 3.12EE40 pKa = 4.28LTLAQRR46 pKa = 11.84LGIDD50 pKa = 3.35VPTEE54 pKa = 4.09EE55 pKa = 4.45EE56 pKa = 4.41CLL58 pKa = 3.67

Molecular weight: 6.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N842|A0A5B9N842_9CAUD DNA-directed DNA polymerase OS=Stenotrophomonas phage Ponderosa OX=2591103 GN=CPT_Ponderosa_022 PE=3 SV=1
MM1 pKa = 7.67GIWEE5 pKa = 4.28LAKK8 pKa = 10.83KK9 pKa = 10.05LGKK12 pKa = 10.54GVVNMLHH19 pKa = 7.03RR20 pKa = 11.84PTKK23 pKa = 10.24HH24 pKa = 5.22VFAVHH29 pKa = 6.89PSTSEE34 pKa = 3.69EE35 pKa = 3.85VRR37 pKa = 11.84QGLRR41 pKa = 11.84STGAEE46 pKa = 4.01VVEE49 pKa = 4.7TKK51 pKa = 10.26PLPRR55 pKa = 11.84EE56 pKa = 4.17KK57 pKa = 9.26PTLMQTFGIPYY68 pKa = 9.49NKK70 pKa = 9.16IAKK73 pKa = 9.74RR74 pKa = 11.84PARR77 pKa = 11.84YY78 pKa = 8.6RR79 pKa = 11.84QGYY82 pKa = 8.98ILGEE86 pKa = 4.13TFDD89 pKa = 4.6LGINKK94 pKa = 8.99VKK96 pKa = 10.4RR97 pKa = 11.84AIRR100 pKa = 11.84ALPKK104 pKa = 9.98KK105 pKa = 7.0EE106 pKa = 3.41QRR108 pKa = 11.84AARR111 pKa = 11.84TAFIKK116 pKa = 10.41NLRR119 pKa = 11.84ASGVKK124 pKa = 9.67NN125 pKa = 3.43

Molecular weight: 14.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13521

44

1360

250.4

27.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.413 ± 0.494

0.836 ± 0.139

6.035 ± 0.249

6.323 ± 0.272

3.587 ± 0.098

7.684 ± 0.38

2.093 ± 0.17

4.571 ± 0.197

5.732 ± 0.263

7.891 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.461 ± 0.235

4.186 ± 0.206

4.201 ± 0.273

4.068 ± 0.241

5.932 ± 0.265

5.295 ± 0.234

5.754 ± 0.245

6.723 ± 0.344

1.694 ± 0.105

3.52 ± 0.204
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski