Amino acid dipepetide frequency for Stenotrophomonas phage Ponderosa

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.648AlaAla: 12.648 ± 1.579
1.331AlaCys: 1.331 ± 0.348
5.991AlaAsp: 5.991 ± 0.67
6.953AlaGlu: 6.953 ± 0.735
2.367AlaPhe: 2.367 ± 0.396
8.062AlaGly: 8.062 ± 1.285
1.923AlaHis: 1.923 ± 0.355
4.882AlaIle: 4.882 ± 0.657
6.361AlaLys: 6.361 ± 0.696
8.062AlaLeu: 8.062 ± 0.893
3.698AlaMet: 3.698 ± 0.59
4.142AlaAsn: 4.142 ± 0.65
4.586AlaPro: 4.586 ± 0.592
4.882AlaGln: 4.882 ± 0.934
5.621AlaArg: 5.621 ± 0.742
4.882AlaSer: 4.882 ± 0.584
5.621AlaThr: 5.621 ± 0.763
6.805AlaVal: 6.805 ± 0.572
1.775AlaTrp: 1.775 ± 0.354
3.55AlaTyr: 3.55 ± 0.592
0.0AlaXaa: 0.0 ± 0.0
Cys
0.814CysAla: 0.814 ± 0.33
0.222CysCys: 0.222 ± 0.117
0.37CysAsp: 0.37 ± 0.202
0.592CysGlu: 0.592 ± 0.233
0.074CysPhe: 0.074 ± 0.082
0.74CysGly: 0.74 ± 0.344
0.444CysHis: 0.444 ± 0.196
0.518CysIle: 0.518 ± 0.277
0.444CysLys: 0.444 ± 0.155
1.036CysLeu: 1.036 ± 0.312
0.296CysMet: 0.296 ± 0.126
0.444CysAsn: 0.444 ± 0.192
0.074CysPro: 0.074 ± 0.082
0.148CysGln: 0.148 ± 0.09
0.37CysArg: 0.37 ± 0.191
0.444CysSer: 0.444 ± 0.223
0.296CysThr: 0.296 ± 0.139
0.74CysVal: 0.74 ± 0.228
0.148CysTrp: 0.148 ± 0.097
0.148CysTyr: 0.148 ± 0.113
0.0CysXaa: 0.0 ± 0.0
Asp
6.731AspAla: 6.731 ± 0.844
0.074AspCys: 0.074 ± 0.064
3.698AspAsp: 3.698 ± 0.577
4.068AspGlu: 4.068 ± 0.604
2.293AspPhe: 2.293 ± 0.367
5.251AspGly: 5.251 ± 0.578
1.553AspHis: 1.553 ± 0.468
3.328AspIle: 3.328 ± 0.503
3.328AspLys: 3.328 ± 0.441
2.885AspLeu: 2.885 ± 0.476
2.589AspMet: 2.589 ± 0.487
2.811AspAsn: 2.811 ± 0.516
3.254AspPro: 3.254 ± 0.636
2.071AspGln: 2.071 ± 0.411
4.142AspArg: 4.142 ± 0.546
2.811AspSer: 2.811 ± 0.523
2.589AspThr: 2.589 ± 0.405
3.772AspVal: 3.772 ± 0.55
1.036AspTrp: 1.036 ± 0.395
2.071AspTyr: 2.071 ± 0.4
0.0AspXaa: 0.0 ± 0.0
Glu
7.101GluAla: 7.101 ± 0.724
0.444GluCys: 0.444 ± 0.188
3.624GluAsp: 3.624 ± 0.492
3.994GluGlu: 3.994 ± 0.717
2.811GluPhe: 2.811 ± 0.472
4.364GluGly: 4.364 ± 0.618
1.553GluHis: 1.553 ± 0.372
1.479GluIle: 1.479 ± 0.349
2.959GluLys: 2.959 ± 0.439
5.991GluLeu: 5.991 ± 0.707
1.923GluMet: 1.923 ± 0.344
2.293GluAsn: 2.293 ± 0.36
2.589GluPro: 2.589 ± 0.444
3.92GluGln: 3.92 ± 0.456
3.772GluArg: 3.772 ± 0.692
2.811GluSer: 2.811 ± 0.495
2.737GluThr: 2.737 ± 0.375
4.956GluVal: 4.956 ± 0.629
1.479GluTrp: 1.479 ± 0.259
2.441GluTyr: 2.441 ± 0.514
0.0GluXaa: 0.0 ± 0.0
Phe
3.476PheAla: 3.476 ± 0.528
0.222PheCys: 0.222 ± 0.132
3.92PheAsp: 3.92 ± 0.51
2.515PheGlu: 2.515 ± 0.346
0.74PhePhe: 0.74 ± 0.224
2.663PheGly: 2.663 ± 0.495
0.518PheHis: 0.518 ± 0.18
2.071PheIle: 2.071 ± 0.435
1.849PheLys: 1.849 ± 0.323
2.441PheLeu: 2.441 ± 0.367
0.888PheMet: 0.888 ± 0.259
1.849PheAsn: 1.849 ± 0.372
1.627PhePro: 1.627 ± 0.374
1.479PheGln: 1.479 ± 0.283
1.553PheArg: 1.553 ± 0.318
1.627PheSer: 1.627 ± 0.336
1.701PheThr: 1.701 ± 0.29
2.219PheVal: 2.219 ± 0.47
0.444PheTrp: 0.444 ± 0.22
2.071PheTyr: 2.071 ± 0.295
0.0PheXaa: 0.0 ± 0.0
Gly
7.101GlyAla: 7.101 ± 0.742
0.444GlyCys: 0.444 ± 0.17
4.512GlyAsp: 4.512 ± 0.576
4.808GlyGlu: 4.808 ± 0.69
2.811GlyPhe: 2.811 ± 0.403
6.731GlyGly: 6.731 ± 0.795
1.036GlyHis: 1.036 ± 0.273
4.142GlyIle: 4.142 ± 0.657
6.287GlyLys: 6.287 ± 0.652
6.213GlyLeu: 6.213 ± 0.797
3.55GlyMet: 3.55 ± 0.429
3.772GlyAsn: 3.772 ± 0.43
2.071GlyPro: 2.071 ± 0.341
1.849GlyGln: 1.849 ± 0.32
4.66GlyArg: 4.66 ± 0.594
4.142GlySer: 4.142 ± 0.427
4.068GlyThr: 4.068 ± 0.564
4.66GlyVal: 4.66 ± 0.575
1.627GlyTrp: 1.627 ± 0.368
2.367GlyTyr: 2.367 ± 0.392
0.0GlyXaa: 0.0 ± 0.0
His
2.071HisAla: 2.071 ± 0.438
0.148HisCys: 0.148 ± 0.111
1.109HisAsp: 1.109 ± 0.293
1.405HisGlu: 1.405 ± 0.378
0.74HisPhe: 0.74 ± 0.204
1.627HisGly: 1.627 ± 0.356
0.296HisHis: 0.296 ± 0.148
0.74HisIle: 0.74 ± 0.199
1.479HisLys: 1.479 ± 0.348
1.627HisLeu: 1.627 ± 0.332
0.666HisMet: 0.666 ± 0.17
0.962HisAsn: 0.962 ± 0.254
1.183HisPro: 1.183 ± 0.224
0.74HisGln: 0.74 ± 0.175
1.627HisArg: 1.627 ± 0.348
0.592HisSer: 0.592 ± 0.258
1.775HisThr: 1.775 ± 0.343
0.962HisVal: 0.962 ± 0.265
0.222HisTrp: 0.222 ± 0.127
0.962HisTyr: 0.962 ± 0.365
0.0HisXaa: 0.0 ± 0.0
Ile
4.734IleAla: 4.734 ± 0.632
0.592IleCys: 0.592 ± 0.214
3.328IleAsp: 3.328 ± 0.574
3.994IleGlu: 3.994 ± 0.546
1.036IlePhe: 1.036 ± 0.253
3.624IleGly: 3.624 ± 0.559
1.331IleHis: 1.331 ± 0.263
1.849IleIle: 1.849 ± 0.276
2.663IleLys: 2.663 ± 0.472
2.589IleLeu: 2.589 ± 0.388
1.109IleMet: 1.109 ± 0.324
1.923IleAsn: 1.923 ± 0.398
1.701IlePro: 1.701 ± 0.413
1.553IleGln: 1.553 ± 0.338
3.402IleArg: 3.402 ± 0.369
2.219IleSer: 2.219 ± 0.377
3.476IleThr: 3.476 ± 0.441
2.737IleVal: 2.737 ± 0.584
1.036IleTrp: 1.036 ± 0.293
0.814IleTyr: 0.814 ± 0.273
0.0IleXaa: 0.0 ± 0.0
Lys
6.435LysAla: 6.435 ± 0.826
0.222LysCys: 0.222 ± 0.124
2.885LysAsp: 2.885 ± 0.435
3.254LysGlu: 3.254 ± 0.511
2.885LysPhe: 2.885 ± 0.489
4.068LysGly: 4.068 ± 0.591
1.553LysHis: 1.553 ± 0.33
1.775LysIle: 1.775 ± 0.353
2.885LysLys: 2.885 ± 0.474
5.325LysLeu: 5.325 ± 0.675
1.405LysMet: 1.405 ± 0.301
2.885LysAsn: 2.885 ± 0.523
2.219LysPro: 2.219 ± 0.425
2.293LysGln: 2.293 ± 0.355
3.402LysArg: 3.402 ± 0.546
3.254LysSer: 3.254 ± 0.439
2.589LysThr: 2.589 ± 0.391
4.216LysVal: 4.216 ± 0.533
1.257LysTrp: 1.257 ± 0.283
2.515LysTyr: 2.515 ± 0.463
0.0LysXaa: 0.0 ± 0.0
Leu
7.84LeuAla: 7.84 ± 0.778
0.518LeuCys: 0.518 ± 0.218
5.325LeuAsp: 5.325 ± 0.518
4.808LeuGlu: 4.808 ± 0.525
2.737LeuPhe: 2.737 ± 0.381
5.473LeuGly: 5.473 ± 0.775
1.849LeuHis: 1.849 ± 0.311
3.402LeuIle: 3.402 ± 0.548
3.92LeuLys: 3.92 ± 0.543
6.657LeuLeu: 6.657 ± 0.926
2.811LeuMet: 2.811 ± 0.358
2.663LeuAsn: 2.663 ± 0.474
3.328LeuPro: 3.328 ± 0.495
3.328LeuGln: 3.328 ± 0.643
5.991LeuArg: 5.991 ± 0.595
5.547LeuSer: 5.547 ± 0.701
5.325LeuThr: 5.325 ± 0.715
4.29LeuVal: 4.29 ± 0.512
1.331LeuTrp: 1.331 ± 0.334
1.775LeuTyr: 1.775 ± 0.278
0.0LeuXaa: 0.0 ± 0.0
Met
3.772MetAla: 3.772 ± 0.546
0.148MetCys: 0.148 ± 0.09
2.589MetAsp: 2.589 ± 0.448
1.849MetGlu: 1.849 ± 0.373
1.036MetPhe: 1.036 ± 0.288
2.071MetGly: 2.071 ± 0.628
0.666MetHis: 0.666 ± 0.233
1.701MetIle: 1.701 ± 0.299
1.701MetLys: 1.701 ± 0.338
2.959MetLeu: 2.959 ± 0.46
0.814MetMet: 0.814 ± 0.175
1.775MetAsn: 1.775 ± 0.488
1.109MetPro: 1.109 ± 0.273
2.071MetGln: 2.071 ± 0.325
2.515MetArg: 2.515 ± 0.542
2.589MetSer: 2.589 ± 0.399
2.367MetThr: 2.367 ± 0.251
1.553MetVal: 1.553 ± 0.287
0.592MetTrp: 0.592 ± 0.218
0.74MetTyr: 0.74 ± 0.23
0.0MetXaa: 0.0 ± 0.0
Asn
4.734AsnAla: 4.734 ± 0.755
0.0AsnCys: 0.0 ± 0.0
2.367AsnAsp: 2.367 ± 0.524
2.219AsnGlu: 2.219 ± 0.552
1.627AsnPhe: 1.627 ± 0.346
3.18AsnGly: 3.18 ± 0.453
0.592AsnHis: 0.592 ± 0.276
2.293AsnIle: 2.293 ± 0.325
2.293AsnLys: 2.293 ± 0.37
3.033AsnLeu: 3.033 ± 0.365
2.293AsnMet: 2.293 ± 0.339
2.071AsnAsn: 2.071 ± 0.414
2.441AsnPro: 2.441 ± 0.437
1.701AsnGln: 1.701 ± 0.383
2.367AsnArg: 2.367 ± 0.381
1.553AsnSer: 1.553 ± 0.347
1.701AsnThr: 1.701 ± 0.392
3.107AsnVal: 3.107 ± 0.526
0.37AsnTrp: 0.37 ± 0.178
1.923AsnTyr: 1.923 ± 0.514
0.0AsnXaa: 0.0 ± 0.0
Pro
3.55ProAla: 3.55 ± 0.533
0.222ProCys: 0.222 ± 0.139
3.328ProAsp: 3.328 ± 0.462
3.254ProGlu: 3.254 ± 0.433
1.109ProPhe: 1.109 ± 0.251
2.959ProGly: 2.959 ± 0.676
0.888ProHis: 0.888 ± 0.356
1.923ProIle: 1.923 ± 0.311
2.737ProLys: 2.737 ± 0.594
3.107ProLeu: 3.107 ± 0.429
1.553ProMet: 1.553 ± 0.3
1.257ProAsn: 1.257 ± 0.286
1.849ProPro: 1.849 ± 0.487
1.183ProGln: 1.183 ± 0.273
1.997ProArg: 1.997 ± 0.338
2.811ProSer: 2.811 ± 0.459
2.663ProThr: 2.663 ± 0.488
3.772ProVal: 3.772 ± 0.657
0.148ProTrp: 0.148 ± 0.098
1.627ProTyr: 1.627 ± 0.34
0.0ProXaa: 0.0 ± 0.0
Gln
4.364GlnAla: 4.364 ± 0.794
0.37GlnCys: 0.37 ± 0.213
1.553GlnAsp: 1.553 ± 0.294
2.071GlnGlu: 2.071 ± 0.414
1.775GlnPhe: 1.775 ± 0.312
3.107GlnGly: 3.107 ± 0.575
0.888GlnHis: 0.888 ± 0.244
1.775GlnIle: 1.775 ± 0.42
2.515GlnLys: 2.515 ± 0.464
3.698GlnLeu: 3.698 ± 0.49
1.849GlnMet: 1.849 ± 0.431
1.405GlnAsn: 1.405 ± 0.322
1.183GlnPro: 1.183 ± 0.385
1.849GlnGln: 1.849 ± 0.554
3.402GlnArg: 3.402 ± 0.579
1.405GlnSer: 1.405 ± 0.412
2.663GlnThr: 2.663 ± 0.486
2.811GlnVal: 2.811 ± 0.53
0.518GlnTrp: 0.518 ± 0.15
1.479GlnTyr: 1.479 ± 0.309
0.0GlnXaa: 0.0 ± 0.0
Arg
6.731ArgAla: 6.731 ± 1.006
0.74ArgCys: 0.74 ± 0.282
2.885ArgAsp: 2.885 ± 0.498
3.92ArgGlu: 3.92 ± 0.545
2.219ArgPhe: 2.219 ± 0.449
4.216ArgGly: 4.216 ± 0.658
1.036ArgHis: 1.036 ± 0.299
3.92ArgIle: 3.92 ± 0.61
3.698ArgLys: 3.698 ± 0.489
4.142ArgLeu: 4.142 ± 0.753
2.515ArgMet: 2.515 ± 0.491
2.145ArgAsn: 2.145 ± 0.42
2.811ArgPro: 2.811 ± 0.434
2.515ArgGln: 2.515 ± 0.411
3.254ArgArg: 3.254 ± 0.46
2.663ArgSer: 2.663 ± 0.344
3.254ArgThr: 3.254 ± 0.488
3.55ArgVal: 3.55 ± 0.541
0.888ArgTrp: 0.888 ± 0.261
2.219ArgTyr: 2.219 ± 0.344
0.0ArgXaa: 0.0 ± 0.0
Ser
5.104SerAla: 5.104 ± 0.487
0.666SerCys: 0.666 ± 0.227
3.254SerAsp: 3.254 ± 0.461
2.515SerGlu: 2.515 ± 0.466
2.367SerPhe: 2.367 ± 0.392
4.66SerGly: 4.66 ± 0.646
0.518SerHis: 0.518 ± 0.189
2.145SerIle: 2.145 ± 0.416
3.033SerLys: 3.033 ± 0.393
4.66SerLeu: 4.66 ± 0.544
1.701SerMet: 1.701 ± 0.355
2.145SerAsn: 2.145 ± 0.493
2.219SerPro: 2.219 ± 0.404
1.923SerGln: 1.923 ± 0.462
2.737SerArg: 2.737 ± 0.455
2.663SerSer: 2.663 ± 0.491
3.55SerThr: 3.55 ± 0.476
4.29SerVal: 4.29 ± 0.509
1.183SerTrp: 1.183 ± 0.246
1.627SerTyr: 1.627 ± 0.424
0.0SerXaa: 0.0 ± 0.0
Thr
5.03ThrAla: 5.03 ± 0.563
0.518ThrCys: 0.518 ± 0.186
3.846ThrAsp: 3.846 ± 0.602
2.663ThrGlu: 2.663 ± 0.475
1.849ThrPhe: 1.849 ± 0.349
5.473ThrGly: 5.473 ± 0.61
1.036ThrHis: 1.036 ± 0.339
3.18ThrIle: 3.18 ± 0.604
3.328ThrLys: 3.328 ± 0.418
5.03ThrLeu: 5.03 ± 0.683
1.775ThrMet: 1.775 ± 0.461
2.071ThrAsn: 2.071 ± 0.358
2.737ThrPro: 2.737 ± 0.54
2.293ThrGln: 2.293 ± 0.484
2.145ThrArg: 2.145 ± 0.35
3.18ThrSer: 3.18 ± 0.424
3.846ThrThr: 3.846 ± 0.6
4.438ThrVal: 4.438 ± 0.733
0.962ThrTrp: 0.962 ± 0.265
2.145ThrTyr: 2.145 ± 0.407
0.0ThrXaa: 0.0 ± 0.0
Val
6.213ValAla: 6.213 ± 0.737
0.666ValCys: 0.666 ± 0.251
2.885ValAsp: 2.885 ± 0.46
5.03ValGlu: 5.03 ± 0.741
2.145ValPhe: 2.145 ± 0.347
4.882ValGly: 4.882 ± 0.587
1.849ValHis: 1.849 ± 0.327
2.737ValIle: 2.737 ± 0.426
3.476ValLys: 3.476 ± 0.453
5.178ValLeu: 5.178 ± 0.735
1.775ValMet: 1.775 ± 0.307
3.254ValAsn: 3.254 ± 0.454
3.033ValPro: 3.033 ± 0.574
2.885ValGln: 2.885 ± 0.536
3.624ValArg: 3.624 ± 0.471
4.068ValSer: 4.068 ± 0.811
4.438ValThr: 4.438 ± 0.707
5.178ValVal: 5.178 ± 0.925
1.331ValTrp: 1.331 ± 0.383
2.589ValTyr: 2.589 ± 0.426
0.0ValXaa: 0.0 ± 0.0
Trp
1.553TrpAla: 1.553 ± 0.39
0.444TrpCys: 0.444 ± 0.212
0.74TrpAsp: 0.74 ± 0.258
0.888TrpGlu: 0.888 ± 0.238
1.405TrpPhe: 1.405 ± 0.336
0.962TrpGly: 0.962 ± 0.231
0.666TrpHis: 0.666 ± 0.193
0.666TrpIle: 0.666 ± 0.28
0.74TrpLys: 0.74 ± 0.27
1.775TrpLeu: 1.775 ± 0.309
0.518TrpMet: 0.518 ± 0.204
0.74TrpAsn: 0.74 ± 0.217
0.37TrpPro: 0.37 ± 0.17
0.74TrpGln: 0.74 ± 0.251
0.814TrpArg: 0.814 ± 0.204
0.962TrpSer: 0.962 ± 0.245
0.74TrpThr: 0.74 ± 0.221
1.109TrpVal: 1.109 ± 0.254
0.222TrpTrp: 0.222 ± 0.132
0.888TrpTyr: 0.888 ± 0.262
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.142TyrAla: 4.142 ± 0.547
0.37TyrCys: 0.37 ± 0.176
2.145TyrAsp: 2.145 ± 0.416
2.441TyrGlu: 2.441 ± 0.446
1.849TyrPhe: 1.849 ± 0.433
2.737TyrGly: 2.737 ± 0.415
0.74TyrHis: 0.74 ± 0.203
1.183TyrIle: 1.183 ± 0.275
1.627TyrLys: 1.627 ± 0.309
2.515TyrLeu: 2.515 ± 0.592
0.814TyrMet: 0.814 ± 0.204
1.257TyrAsn: 1.257 ± 0.303
1.627TyrPro: 1.627 ± 0.406
1.257TyrGln: 1.257 ± 0.243
1.923TyrArg: 1.923 ± 0.382
2.737TyrSer: 2.737 ± 0.324
2.145TyrThr: 2.145 ± 0.477
2.071TyrVal: 2.071 ± 0.35
0.37TyrTrp: 0.37 ± 0.137
1.257TyrTyr: 1.257 ± 0.349
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (13521 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski