Olsenella sp. An188
Average proteome isoelectric point is 5.64
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1814 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y4K9Q7|A0A1Y4K9Q7_9ACTN Dihydrodipicolinate synthase family protein OS=Olsenella sp. An188 OX=1965579 GN=B5F23_01925 PE=3 SV=1
MM1 pKa = 7.38 SNISRR6 pKa = 11.84 RR7 pKa = 11.84 TFLAGSSASLLAVFLGGCTGSNEE30 pKa = 3.82 PAAEE34 pKa = 4.36 GEE36 pKa = 4.43 GTEE39 pKa = 4.25 AAAGGHH45 pKa = 6.24 IYY47 pKa = 10.97 VLTASPDD54 pKa = 3.67 HH55 pKa = 6.45 GWTGQVGVFAQEE67 pKa = 4.24 KK68 pKa = 8.42 VDD70 pKa = 3.8 EE71 pKa = 4.37 VNEE74 pKa = 4.3 AGVYY78 pKa = 7.97 TAEE81 pKa = 4.31 LQTAGAAADD90 pKa = 3.83 QIAQVEE96 pKa = 4.67 DD97 pKa = 3.61 ILAGDD102 pKa = 4.33 LSGVAGIVIQPLDD115 pKa = 3.95 DD116 pKa = 3.77 TVQSAIQQIVDD127 pKa = 3.13 AGVAYY132 pKa = 10.52 VAFDD136 pKa = 4.56 RR137 pKa = 11.84 IIEE140 pKa = 4.23 AVQDD144 pKa = 3.67 SAVANVKK151 pKa = 10.13 GDD153 pKa = 3.47 NSGIGAGSAAYY164 pKa = 9.38 FVEE167 pKa = 4.77 NGMKK171 pKa = 10.29 PGDD174 pKa = 3.48 AVYY177 pKa = 9.8 IYY179 pKa = 10.58 QGDD182 pKa = 3.95 TSSVTTLRR190 pKa = 11.84 DD191 pKa = 3.34 NGFTDD196 pKa = 3.72 YY197 pKa = 11.7 LLGNLEE203 pKa = 4.05 FDD205 pKa = 3.81 GATVPEE211 pKa = 4.37 DD212 pKa = 3.64 AKK214 pKa = 9.42 WTQEE218 pKa = 4.14 QIDD221 pKa = 3.97 SAITKK226 pKa = 9.71 SGAMNWSRR234 pKa = 11.84 SDD236 pKa = 3.05 TKK238 pKa = 11.18 AAFEE242 pKa = 4.35 ALLGDD247 pKa = 4.08 EE248 pKa = 5.03 ANASIKK254 pKa = 8.48 WWYY257 pKa = 10.82 AEE259 pKa = 4.09 DD260 pKa = 5.16 DD261 pKa = 4.0 EE262 pKa = 5.02 LAMGILEE269 pKa = 4.42 ALNGGGISDD278 pKa = 3.84 SAKK281 pKa = 8.93 EE282 pKa = 3.77 AFYY285 pKa = 9.38 ATKK288 pKa = 10.34 PFITGCGGLQEE299 pKa = 4.55 YY300 pKa = 10.58 YY301 pKa = 10.84 DD302 pKa = 4.27 VISGEE307 pKa = 4.49 SYY309 pKa = 11.26 TDD311 pKa = 2.86 ITANLGGVMSTTYY324 pKa = 10.04 SPSMIQTAIQDD335 pKa = 4.12 MVDD338 pKa = 3.37 HH339 pKa = 7.28 LDD341 pKa = 3.86 GKK343 pKa = 9.62 EE344 pKa = 3.92 VPQDD348 pKa = 3.41 HH349 pKa = 7.11 VIACEE354 pKa = 3.73 NVTAEE359 pKa = 4.12 NVADD363 pKa = 3.85 YY364 pKa = 11.46 VGFEE368 pKa = 3.98
Molecular weight: 38.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.592
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.961
Wikipedia 3.706
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.719
Sillero 3.923
Patrickios 1.265
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|A0A1Y4K5A3|A0A1Y4K5A3_9ACTN Uncharacterized protein OS=Olsenella sp. An188 OX=1965579 GN=B5F23_04275 PE=4 SV=1
MM1 pKa = 7.48 TFGTTTPRR9 pKa = 11.84 PRR11 pKa = 11.84 PPSRR15 pKa = 11.84 ARR17 pKa = 11.84 ARR19 pKa = 11.84 VRR21 pKa = 11.84 GRR23 pKa = 11.84 TSRR26 pKa = 11.84 SPRR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 RR32 pKa = 11.84 SRR34 pKa = 11.84 RR35 pKa = 11.84 TAPPPPRR42 pKa = 11.84 GCRR45 pKa = 11.84 RR46 pKa = 11.84 RR47 pKa = 11.84 RR48 pKa = 11.84 RR49 pKa = 11.84 SRR51 pKa = 11.84 RR52 pKa = 11.84 SRR54 pKa = 11.84 CRR56 pKa = 11.84 RR57 pKa = 11.84 WAPRR61 pKa = 11.84 RR62 pKa = 11.84 TPAQTSRR69 pKa = 11.84 GPRR72 pKa = 11.84 RR73 pKa = 11.84 PRR75 pKa = 11.84 RR76 pKa = 11.84 RR77 pKa = 11.84 RR78 pKa = 11.84 RR79 pKa = 11.84 ATAGPRR85 pKa = 11.84 SGG87 pKa = 3.92
Molecular weight: 10.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.525
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.544
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.252
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.239
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1814
0
1814
615698
36
2282
339.4
36.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.452 ± 0.084
1.517 ± 0.024
6.259 ± 0.043
7.062 ± 0.049
3.286 ± 0.034
8.786 ± 0.053
1.809 ± 0.023
3.942 ± 0.053
2.63 ± 0.046
9.684 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.394 ± 0.027
2.327 ± 0.032
4.436 ± 0.035
2.467 ± 0.03
7.27 ± 0.07
5.744 ± 0.041
5.157 ± 0.052
9.042 ± 0.051
1.113 ± 0.019
2.623 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here