Amino acid dipepetide frequency for Olsenella sp. An188

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.991AlaAla: 16.991 ± 0.256
2.212AlaCys: 2.212 ± 0.068
7.596AlaAsp: 7.596 ± 0.109
8.863AlaGlu: 8.863 ± 0.163
3.848AlaPhe: 3.848 ± 0.091
11.121AlaGly: 11.121 ± 0.17
2.271AlaHis: 2.271 ± 0.067
4.569AlaIle: 4.569 ± 0.1
2.997AlaLys: 2.997 ± 0.082
13.718AlaLeu: 13.718 ± 0.225
2.868AlaMet: 2.868 ± 0.075
2.581AlaAsn: 2.581 ± 0.069
4.967AlaPro: 4.967 ± 0.111
3.416AlaGln: 3.416 ± 0.081
9.846AlaArg: 9.846 ± 0.19
6.807AlaSer: 6.807 ± 0.126
5.86AlaThr: 5.86 ± 0.111
9.461AlaVal: 9.461 ± 0.168
1.46AlaTrp: 1.46 ± 0.054
3.068AlaTyr: 3.068 ± 0.073
0.0AlaXaa: 0.0 ± 0.0
Cys
1.964CysAla: 1.964 ± 0.061
0.271CysCys: 0.271 ± 0.02
1.074CysAsp: 1.074 ± 0.045
1.124CysGlu: 1.124 ± 0.045
0.403CysPhe: 0.403 ± 0.026
1.752CysGly: 1.752 ± 0.056
0.318CysHis: 0.318 ± 0.025
0.447CysIle: 0.447 ± 0.027
0.286CysLys: 0.286 ± 0.022
1.226CysLeu: 1.226 ± 0.046
0.292CysMet: 0.292 ± 0.023
0.279CysAsn: 0.279 ± 0.023
0.838CysPro: 0.838 ± 0.038
0.292CysGln: 0.292 ± 0.023
0.96CysArg: 0.96 ± 0.043
0.841CysSer: 0.841 ± 0.04
0.723CysThr: 0.723 ± 0.043
1.53CysVal: 1.53 ± 0.056
0.161CysTrp: 0.161 ± 0.017
0.385CysTyr: 0.385 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
8.597AspAla: 8.597 ± 0.138
0.75AspCys: 0.75 ± 0.039
3.947AspAsp: 3.947 ± 0.122
5.941AspGlu: 5.941 ± 0.104
2.318AspPhe: 2.318 ± 0.062
5.709AspGly: 5.709 ± 0.121
1.062AspHis: 1.062 ± 0.039
2.227AspIle: 2.227 ± 0.062
1.48AspLys: 1.48 ± 0.052
5.845AspLeu: 5.845 ± 0.106
1.32AspMet: 1.32 ± 0.048
1.317AspAsn: 1.317 ± 0.042
3.2AspPro: 3.2 ± 0.073
1.369AspGln: 1.369 ± 0.052
3.533AspArg: 3.533 ± 0.078
2.639AspSer: 2.639 ± 0.068
2.552AspThr: 2.552 ± 0.077
6.198AspVal: 6.198 ± 0.096
0.765AspTrp: 0.765 ± 0.044
1.826AspTyr: 1.826 ± 0.066
0.0AspXaa: 0.0 ± 0.0
Glu
8.366GluAla: 8.366 ± 0.133
0.833GluCys: 0.833 ± 0.039
3.776GluAsp: 3.776 ± 0.078
4.679GluGlu: 4.679 ± 0.116
1.907GluPhe: 1.907 ± 0.059
6.149GluGly: 6.149 ± 0.104
1.455GluHis: 1.455 ± 0.055
2.589GluIle: 2.589 ± 0.072
2.92GluLys: 2.92 ± 0.081
8.147GluLeu: 8.147 ± 0.115
1.73GluMet: 1.73 ± 0.054
1.707GluAsn: 1.707 ± 0.052
2.95GluPro: 2.95 ± 0.079
2.246GluGln: 2.246 ± 0.061
7.223GluArg: 7.223 ± 0.138
3.036GluSer: 3.036 ± 0.082
3.019GluThr: 3.019 ± 0.075
5.779GluVal: 5.779 ± 0.09
0.689GluTrp: 0.689 ± 0.033
1.42GluTyr: 1.42 ± 0.048
0.0GluXaa: 0.0 ± 0.0
Phe
3.692PheAla: 3.692 ± 0.085
0.637PheCys: 0.637 ± 0.029
2.573PheAsp: 2.573 ± 0.062
2.287PheGlu: 2.287 ± 0.056
1.208PhePhe: 1.208 ± 0.054
3.209PheGly: 3.209 ± 0.074
0.564PheHis: 0.564 ± 0.029
1.09PheIle: 1.09 ± 0.051
0.799PheLys: 0.799 ± 0.036
2.745PheLeu: 2.745 ± 0.064
0.781PheMet: 0.781 ± 0.032
0.806PheAsn: 0.806 ± 0.04
1.186PhePro: 1.186 ± 0.041
0.711PheGln: 0.711 ± 0.032
1.735PheArg: 1.735 ± 0.058
2.644PheSer: 2.644 ± 0.069
1.733PheThr: 1.733 ± 0.055
3.174PheVal: 3.174 ± 0.075
0.382PheTrp: 0.382 ± 0.024
0.906PheTyr: 0.906 ± 0.041
0.0PheXaa: 0.0 ± 0.0
Gly
10.106GlyAla: 10.106 ± 0.139
1.473GlyCys: 1.473 ± 0.049
4.871GlyAsp: 4.871 ± 0.099
6.454GlyGlu: 6.454 ± 0.108
3.003GlyPhe: 3.003 ± 0.064
7.281GlyGly: 7.281 ± 0.121
1.704GlyHis: 1.704 ± 0.047
4.036GlyIle: 4.036 ± 0.083
2.576GlyLys: 2.576 ± 0.078
8.056GlyLeu: 8.056 ± 0.133
2.35GlyMet: 2.35 ± 0.053
1.928GlyAsn: 1.928 ± 0.058
2.802GlyPro: 2.802 ± 0.075
2.261GlyGln: 2.261 ± 0.069
6.619GlyArg: 6.619 ± 0.121
5.335GlySer: 5.335 ± 0.098
5.235GlyThr: 5.235 ± 0.109
8.033GlyVal: 8.033 ± 0.117
1.147GlyTrp: 1.147 ± 0.044
2.592GlyTyr: 2.592 ± 0.07
0.0GlyXaa: 0.0 ± 0.0
His
2.098HisAla: 2.098 ± 0.065
0.247HisCys: 0.247 ± 0.021
1.286HisAsp: 1.286 ± 0.048
1.335HisGlu: 1.335 ± 0.048
0.642HisPhe: 0.642 ± 0.029
1.741HisGly: 1.741 ± 0.061
0.409HisHis: 0.409 ± 0.03
0.684HisIle: 0.684 ± 0.038
0.417HisLys: 0.417 ± 0.026
1.686HisLeu: 1.686 ± 0.047
0.344HisMet: 0.344 ± 0.022
0.416HisAsn: 0.416 ± 0.03
1.041HisPro: 1.041 ± 0.043
0.385HisGln: 0.385 ± 0.023
1.135HisArg: 1.135 ± 0.041
0.745HisSer: 0.745 ± 0.033
0.784HisThr: 0.784 ± 0.035
1.96HisVal: 1.96 ± 0.061
0.166HisTrp: 0.166 ± 0.017
0.565HisTyr: 0.565 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
4.692IleAla: 4.692 ± 0.102
0.705IleCys: 0.705 ± 0.036
2.984IleAsp: 2.984 ± 0.072
2.961IleGlu: 2.961 ± 0.082
1.217IlePhe: 1.217 ± 0.049
3.175IleGly: 3.175 ± 0.081
0.616IleHis: 0.616 ± 0.033
1.662IleIle: 1.662 ± 0.07
1.168IleLys: 1.168 ± 0.05
3.063IleLeu: 3.063 ± 0.077
0.975IleMet: 0.975 ± 0.044
1.137IleAsn: 1.137 ± 0.052
1.92IlePro: 1.92 ± 0.055
0.924IleGln: 0.924 ± 0.045
2.251IleArg: 2.251 ± 0.063
2.54IleSer: 2.54 ± 0.08
2.142IleThr: 2.142 ± 0.069
3.924IleVal: 3.924 ± 0.078
0.364IleTrp: 0.364 ± 0.023
0.999IleTyr: 0.999 ± 0.05
0.0IleXaa: 0.0 ± 0.0
Lys
2.698LysAla: 2.698 ± 0.085
0.3LysCys: 0.3 ± 0.023
1.569LysAsp: 1.569 ± 0.053
1.421LysGlu: 1.421 ± 0.055
0.716LysPhe: 0.716 ± 0.038
2.09LysGly: 2.09 ± 0.076
0.452LysHis: 0.452 ± 0.026
1.543LysIle: 1.543 ± 0.056
1.23LysLys: 1.23 ± 0.058
2.277LysLeu: 2.277 ± 0.066
0.658LysMet: 0.658 ± 0.031
1.035LysAsn: 1.035 ± 0.044
1.199LysPro: 1.199 ± 0.047
0.718LysGln: 0.718 ± 0.03
1.939LysArg: 1.939 ± 0.058
1.584LysSer: 1.584 ± 0.059
1.636LysThr: 1.636 ± 0.053
2.308LysVal: 2.308 ± 0.078
0.24LysTrp: 0.24 ± 0.02
0.69LysTyr: 0.69 ± 0.037
0.0LysXaa: 0.0 ± 0.0
Leu
14.255LeuAla: 14.255 ± 0.223
1.384LeuCys: 1.384 ± 0.05
6.474LeuAsp: 6.474 ± 0.122
6.24LeuGlu: 6.24 ± 0.113
2.951LeuPhe: 2.951 ± 0.068
8.641LeuGly: 8.641 ± 0.149
1.416LeuHis: 1.416 ± 0.048
3.166LeuIle: 3.166 ± 0.074
2.144LeuLys: 2.144 ± 0.068
9.03LeuLeu: 9.03 ± 0.156
2.324LeuMet: 2.324 ± 0.051
1.852LeuAsn: 1.852 ± 0.057
4.523LeuPro: 4.523 ± 0.095
1.752LeuGln: 1.752 ± 0.059
6.573LeuArg: 6.573 ± 0.13
6.264LeuSer: 6.264 ± 0.107
5.261LeuThr: 5.261 ± 0.094
9.531LeuVal: 9.531 ± 0.185
0.9LeuTrp: 0.9 ± 0.036
2.162LeuTyr: 2.162 ± 0.059
0.0LeuXaa: 0.0 ± 0.0
Met
3.023MetAla: 3.023 ± 0.073
0.336MetCys: 0.336 ± 0.023
1.426MetAsp: 1.426 ± 0.05
1.403MetGlu: 1.403 ± 0.053
0.627MetPhe: 0.627 ± 0.032
2.345MetGly: 2.345 ± 0.058
0.377MetHis: 0.377 ± 0.024
0.885MetIle: 0.885 ± 0.041
0.655MetLys: 0.655 ± 0.035
2.202MetLeu: 2.202 ± 0.062
0.585MetMet: 0.585 ± 0.033
0.716MetAsn: 0.716 ± 0.036
1.366MetPro: 1.366 ± 0.051
0.581MetGln: 0.581 ± 0.03
1.97MetArg: 1.97 ± 0.065
1.691MetSer: 1.691 ± 0.053
1.33MetThr: 1.33 ± 0.045
1.777MetVal: 1.777 ± 0.052
0.167MetTrp: 0.167 ± 0.017
0.474MetTyr: 0.474 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.621AsnAla: 2.621 ± 0.072
0.307AsnCys: 0.307 ± 0.021
1.418AsnAsp: 1.418 ± 0.053
1.359AsnGlu: 1.359 ± 0.054
0.776AsnPhe: 0.776 ± 0.034
1.978AsnGly: 1.978 ± 0.071
0.421AsnHis: 0.421 ± 0.027
1.108AsnIle: 1.108 ± 0.042
0.646AsnLys: 0.646 ± 0.035
2.319AsnLeu: 2.319 ± 0.059
0.609AsnMet: 0.609 ± 0.03
0.583AsnAsn: 0.583 ± 0.038
1.558AsnPro: 1.558 ± 0.055
0.603AsnGln: 0.603 ± 0.035
1.346AsnArg: 1.346 ± 0.05
1.075AsnSer: 1.075 ± 0.045
1.137AsnThr: 1.137 ± 0.044
2.392AsnVal: 2.392 ± 0.069
0.263AsnTrp: 0.263 ± 0.02
0.745AsnTyr: 0.745 ± 0.041
0.0AsnXaa: 0.0 ± 0.0
Pro
5.763ProAla: 5.763 ± 0.139
0.624ProCys: 0.624 ± 0.033
3.084ProAsp: 3.084 ± 0.078
4.132ProGlu: 4.132 ± 0.106
1.533ProPhe: 1.533 ± 0.052
4.13ProGly: 4.13 ± 0.098
0.799ProHis: 0.799 ± 0.034
1.558ProIle: 1.558 ± 0.053
1.121ProLys: 1.121 ± 0.053
3.794ProLeu: 3.794 ± 0.072
0.919ProMet: 0.919 ± 0.039
1.035ProAsn: 1.035 ± 0.039
1.187ProPro: 1.187 ± 0.052
1.116ProGln: 1.116 ± 0.053
2.852ProArg: 2.852 ± 0.086
2.711ProSer: 2.711 ± 0.064
2.262ProThr: 2.262 ± 0.064
3.914ProVal: 3.914 ± 0.095
0.586ProTrp: 0.586 ± 0.035
1.244ProTyr: 1.244 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
2.92GlnAla: 2.92 ± 0.077
0.219GlnCys: 0.219 ± 0.021
1.317GlnAsp: 1.317 ± 0.047
1.472GlnGlu: 1.472 ± 0.056
0.733GlnPhe: 0.733 ± 0.036
2.071GlnGly: 2.071 ± 0.067
0.416GlnHis: 0.416 ± 0.028
1.431GlnIle: 1.431 ± 0.051
0.651GlnLys: 0.651 ± 0.032
2.644GlnLeu: 2.644 ± 0.062
0.783GlnMet: 0.783 ± 0.038
0.581GlnAsn: 0.581 ± 0.031
0.944GlnPro: 0.944 ± 0.042
0.861GlnGln: 0.861 ± 0.046
1.817GlnArg: 1.817 ± 0.05
1.236GlnSer: 1.236 ± 0.046
1.39GlnThr: 1.39 ± 0.05
2.357GlnVal: 2.357 ± 0.064
0.229GlnTrp: 0.229 ± 0.018
0.598GlnTyr: 0.598 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
9.253ArgAla: 9.253 ± 0.171
0.931ArgCys: 0.931 ± 0.04
4.267ArgAsp: 4.267 ± 0.099
5.776ArgGlu: 5.776 ± 0.101
2.394ArgPhe: 2.394 ± 0.061
5.654ArgGly: 5.654 ± 0.116
1.47ArgHis: 1.47 ± 0.048
2.808ArgIle: 2.808 ± 0.067
1.761ArgLys: 1.761 ± 0.055
6.929ArgLeu: 6.929 ± 0.143
2.017ArgMet: 2.017 ± 0.058
1.483ArgAsn: 1.483 ± 0.046
3.339ArgPro: 3.339 ± 0.09
1.793ArgGln: 1.793 ± 0.059
6.649ArgArg: 6.649 ± 0.14
3.815ArgSer: 3.815 ± 0.085
3.351ArgThr: 3.351 ± 0.074
6.238ArgVal: 6.238 ± 0.115
0.784ArgTrp: 0.784 ± 0.038
1.985ArgTyr: 1.985 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
6.737SerAla: 6.737 ± 0.131
0.858SerCys: 0.858 ± 0.043
3.321SerAsp: 3.321 ± 0.078
3.672SerGlu: 3.672 ± 0.083
2.232SerPhe: 2.232 ± 0.061
5.626SerGly: 5.626 ± 0.109
1.147SerHis: 1.147 ± 0.042
2.215SerIle: 2.215 ± 0.07
1.364SerLys: 1.364 ± 0.056
5.501SerLeu: 5.501 ± 0.116
1.395SerMet: 1.395 ± 0.044
1.254SerAsn: 1.254 ± 0.052
2.574SerPro: 2.574 ± 0.061
1.429SerGln: 1.429 ± 0.05
3.882SerArg: 3.882 ± 0.082
3.598SerSer: 3.598 ± 0.105
3.045SerThr: 3.045 ± 0.086
4.981SerVal: 4.981 ± 0.085
0.806SerTrp: 0.806 ± 0.035
1.804SerTyr: 1.804 ± 0.065
0.0SerXaa: 0.0 ± 0.0
Thr
5.095ThrAla: 5.095 ± 0.088
0.94ThrCys: 0.94 ± 0.037
3.122ThrAsp: 3.122 ± 0.076
2.699ThrGlu: 2.699 ± 0.068
2.064ThrPhe: 2.064 ± 0.058
4.746ThrGly: 4.746 ± 0.099
1.103ThrHis: 1.103 ± 0.044
2.363ThrIle: 2.363 ± 0.076
1.234ThrLys: 1.234 ± 0.048
5.433ThrLeu: 5.433 ± 0.102
1.176ThrMet: 1.176 ± 0.045
1.32ThrAsn: 1.32 ± 0.047
2.974ThrPro: 2.974 ± 0.076
1.299ThrGln: 1.299 ± 0.044
3.169ThrArg: 3.169 ± 0.073
3.057ThrSer: 3.057 ± 0.073
2.914ThrThr: 2.914 ± 0.08
4.541ThrVal: 4.541 ± 0.106
0.62ThrTrp: 0.62 ± 0.035
1.697ThrTyr: 1.697 ± 0.069
0.0ThrXaa: 0.0 ± 0.0
Val
11.571ValAla: 11.571 ± 0.158
1.603ValCys: 1.603 ± 0.063
5.659ValAsp: 5.659 ± 0.104
6.112ValGlu: 6.112 ± 0.108
2.977ValPhe: 2.977 ± 0.073
7.072ValGly: 7.072 ± 0.126
1.304ValHis: 1.304 ± 0.05
3.882ValIle: 3.882 ± 0.086
1.957ValLys: 1.957 ± 0.059
8.238ValLeu: 8.238 ± 0.127
2.098ValMet: 2.098 ± 0.062
2.198ValAsn: 2.198 ± 0.062
4.179ValPro: 4.179 ± 0.081
1.696ValGln: 1.696 ± 0.059
6.575ValArg: 6.575 ± 0.119
5.915ValSer: 5.915 ± 0.107
5.447ValThr: 5.447 ± 0.104
9.017ValVal: 9.017 ± 0.173
0.945ValTrp: 0.945 ± 0.042
1.978ValTyr: 1.978 ± 0.053
0.0ValXaa: 0.0 ± 0.0
Trp
1.134TrpAla: 1.134 ± 0.044
0.201TrpCys: 0.201 ± 0.019
0.741TrpAsp: 0.741 ± 0.043
0.796TrpGlu: 0.796 ± 0.034
0.388TrpPhe: 0.388 ± 0.029
0.978TrpGly: 0.978 ± 0.04
0.227TrpHis: 0.227 ± 0.018
0.395TrpIle: 0.395 ± 0.026
0.274TrpLys: 0.274 ± 0.023
1.14TrpLeu: 1.14 ± 0.046
0.255TrpMet: 0.255 ± 0.017
0.323TrpAsn: 0.323 ± 0.024
0.491TrpPro: 0.491 ± 0.029
0.403TrpGln: 0.403 ± 0.027
0.898TrpArg: 0.898 ± 0.046
0.627TrpSer: 0.627 ± 0.034
0.491TrpThr: 0.491 ± 0.032
0.887TrpVal: 0.887 ± 0.041
0.171TrpTrp: 0.171 ± 0.017
0.312TrpTyr: 0.312 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.945TyrAla: 2.945 ± 0.074
0.335TyrCys: 0.335 ± 0.024
2.09TyrAsp: 2.09 ± 0.069
1.892TyrGlu: 1.892 ± 0.059
0.924TyrPhe: 0.924 ± 0.041
2.392TyrGly: 2.392 ± 0.069
0.559TyrHis: 0.559 ± 0.033
0.762TyrIle: 0.762 ± 0.043
0.622TyrLys: 0.622 ± 0.037
2.849TyrLeu: 2.849 ± 0.072
0.455TyrMet: 0.455 ± 0.027
0.716TyrAsn: 0.716 ± 0.04
1.129TyrPro: 1.129 ± 0.042
0.814TyrGln: 0.814 ± 0.034
1.725TyrArg: 1.725 ± 0.056
1.281TyrSer: 1.281 ± 0.052
1.255TyrThr: 1.255 ± 0.061
2.42TyrVal: 2.42 ± 0.055
0.286TyrTrp: 0.286 ± 0.022
0.783TyrTyr: 0.783 ± 0.039
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1814 proteins (615698 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski