Beihai noda-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KFY0|A0A1L3KFY0_9VIRU RNA replicase OS=Beihai noda-like virus 5 OX=1922487 PE=3 SV=1
MM1 pKa = 7.82LSRR4 pKa = 11.84LYY6 pKa = 10.55HH7 pKa = 5.19VVEE10 pKa = 4.42EE11 pKa = 4.56EE12 pKa = 4.9VFEE15 pKa = 4.33PWAKK19 pKa = 10.6ALARR23 pKa = 11.84PVLHH27 pKa = 6.24VAHH30 pKa = 6.65PVVTAAYY37 pKa = 9.62DD38 pKa = 3.43VTDD41 pKa = 3.67QVSRR45 pKa = 11.84WLGPGYY51 pKa = 7.9GTCPPLSPAEE61 pKa = 3.93EE62 pKa = 3.89VVAFIRR68 pKa = 11.84SCWPDD73 pKa = 3.62LEE75 pKa = 4.57QIDD78 pKa = 4.15YY79 pKa = 11.17ALLIGCRR86 pKa = 11.84LVLLIAGAVILFNLQRR102 pKa = 11.84KK103 pKa = 6.0VLRR106 pKa = 11.84TISITNPVRR115 pKa = 11.84QYY117 pKa = 11.21VIEE120 pKa = 4.06LSKK123 pKa = 10.63IKK125 pKa = 10.23QYY127 pKa = 11.08PDD129 pKa = 3.42DD130 pKa = 3.83LRR132 pKa = 11.84KK133 pKa = 9.94KK134 pKa = 10.04FRR136 pKa = 11.84NLNVLKK142 pKa = 10.36YY143 pKa = 9.65APSLNGHH150 pKa = 5.11SHH152 pKa = 6.1GVCAAFRR159 pKa = 11.84AAADD163 pKa = 3.55DD164 pKa = 4.33TINTFIQEE172 pKa = 4.04NGFEE176 pKa = 4.34VYY178 pKa = 10.31SVGMSAKK185 pKa = 10.8DD186 pKa = 3.19MDD188 pKa = 4.82LGFEE192 pKa = 5.03GYY194 pKa = 10.49RR195 pKa = 11.84PMVEE199 pKa = 4.26DD200 pKa = 4.08KK201 pKa = 11.51DD202 pKa = 3.57MVMGFKK208 pKa = 10.51CSPVRR213 pKa = 11.84DD214 pKa = 3.49NHH216 pKa = 6.17IIKK219 pKa = 9.63MVDD222 pKa = 2.31VDD224 pKa = 4.24YY225 pKa = 11.49YY226 pKa = 11.83VDD228 pKa = 3.85LLYY231 pKa = 10.15WLSFGRR237 pKa = 11.84PVIIYY242 pKa = 7.24TFQPSAVAGMTDD254 pKa = 3.62DD255 pKa = 4.69ASFTIKK261 pKa = 10.71DD262 pKa = 3.92DD263 pKa = 3.65VVHH266 pKa = 6.6FSVSGGARR274 pKa = 11.84WQHH277 pKa = 5.49RR278 pKa = 11.84VWDD281 pKa = 3.99YY282 pKa = 11.73SADD285 pKa = 3.62CVYY288 pKa = 10.71VPSYY292 pKa = 10.17WNSLYY297 pKa = 10.89SYY299 pKa = 11.21VCFCEE304 pKa = 4.61SIQVSHH310 pKa = 7.37DD311 pKa = 3.21RR312 pKa = 11.84RR313 pKa = 11.84IVGLFPRR320 pKa = 11.84TKK322 pKa = 8.93MFSPMEE328 pKa = 4.2SFRR331 pKa = 11.84EE332 pKa = 3.99INPLQRR338 pKa = 11.84MRR340 pKa = 11.84YY341 pKa = 6.81TYY343 pKa = 11.21GRR345 pKa = 11.84FNVLRR350 pKa = 11.84VEE352 pKa = 4.55NKK354 pKa = 10.21GKK356 pKa = 8.8VHH358 pKa = 6.6YY359 pKa = 10.53SIGYY363 pKa = 8.0NGSAVSVDD371 pKa = 3.01INADD375 pKa = 3.39DD376 pKa = 4.69FEE378 pKa = 4.41ATRR381 pKa = 11.84IRR383 pKa = 11.84LEE385 pKa = 3.94NSKK388 pKa = 10.81KK389 pKa = 10.1PVISDD394 pKa = 3.06VEE396 pKa = 4.42RR397 pKa = 11.84ILRR400 pKa = 11.84ASSHH404 pKa = 5.66PQPVLAAAILKK415 pKa = 9.61EE416 pKa = 4.24FLDD419 pKa = 4.18KK420 pKa = 10.87TPQLPRR426 pKa = 11.84QRR428 pKa = 11.84VVSVGQPKK436 pKa = 10.25NEE438 pKa = 3.93RR439 pKa = 11.84GYY441 pKa = 11.07QSLTPLVTEE450 pKa = 4.6DD451 pKa = 3.54GKK453 pKa = 11.0NKK455 pKa = 9.8VKK457 pKa = 10.43EE458 pKa = 3.81IRR460 pKa = 11.84GAICKK465 pKa = 10.13GAVHH469 pKa = 6.61PVSSYY474 pKa = 11.55NNDD477 pKa = 2.79VRR479 pKa = 11.84CVDD482 pKa = 3.36KK483 pKa = 11.17RR484 pKa = 11.84IVAVANNTVFPQRR497 pKa = 11.84YY498 pKa = 6.95QRR500 pKa = 11.84YY501 pKa = 7.99ACAFVKK507 pKa = 10.51LLIPSDD513 pKa = 3.5QVEE516 pKa = 4.3RR517 pKa = 11.84GFPLALSDD525 pKa = 4.06VFDD528 pKa = 4.33RR529 pKa = 11.84QDD531 pKa = 3.35RR532 pKa = 11.84PSQRR536 pKa = 11.84AGFEE540 pKa = 4.16QNHH543 pKa = 4.71TTFFSTDD550 pKa = 2.79NRR552 pKa = 11.84IKK554 pKa = 10.87AFQKK558 pKa = 10.17SEE560 pKa = 3.71AYY562 pKa = 10.64ANVNDD567 pKa = 4.3PRR569 pKa = 11.84NISTTTVDD577 pKa = 2.8SRR579 pKa = 11.84VRR581 pKa = 11.84YY582 pKa = 10.06SSFTLPLAEE591 pKa = 5.2HH592 pKa = 6.36IKK594 pKa = 10.45KK595 pKa = 10.51FEE597 pKa = 3.83WYY599 pKa = 10.5AFGRR603 pKa = 11.84TPKK606 pKa = 9.72VWTEE610 pKa = 3.92MFHH613 pKa = 8.3VMASNSEE620 pKa = 4.23WILATDD626 pKa = 3.65YY627 pKa = 11.5SRR629 pKa = 11.84FDD631 pKa = 3.37GTISKK636 pKa = 10.35SFHH639 pKa = 5.66TLLRR643 pKa = 11.84LPILRR648 pKa = 11.84RR649 pKa = 11.84FFSPEE654 pKa = 3.46YY655 pKa = 9.85HH656 pKa = 6.88PEE658 pKa = 3.89VLKK661 pKa = 10.9LAEE664 pKa = 4.04QQRR667 pKa = 11.84NCPASTAHH675 pKa = 5.92GVTYY679 pKa = 10.68NSGYY683 pKa = 8.81GTVSGSADD691 pKa = 2.93TSLFNTIFNAFVAFVSICEE710 pKa = 3.93TRR712 pKa = 11.84MRR714 pKa = 11.84SLSCDD719 pKa = 2.88MAMTGKK725 pKa = 9.65PFMEE729 pKa = 4.26LVEE732 pKa = 4.45GAFEE736 pKa = 3.91EE737 pKa = 4.99LEE739 pKa = 4.19RR740 pKa = 11.84SCLFGGDD747 pKa = 4.48DD748 pKa = 6.64GILCDD753 pKa = 4.49CEE755 pKa = 4.86AGEE758 pKa = 5.01LEE760 pKa = 4.47SAAEE764 pKa = 4.19KK765 pKa = 10.2MGLKK769 pKa = 10.37LKK771 pKa = 10.63LDD773 pKa = 3.85VYY775 pKa = 10.87HH776 pKa = 6.39YY777 pKa = 11.2GPVPFLGRR785 pKa = 11.84WFVNPWVSGEE795 pKa = 4.08CMADD799 pKa = 3.27VQRR802 pKa = 11.84QVRR805 pKa = 11.84KK806 pKa = 9.91FGLTGSNLEE815 pKa = 4.07EE816 pKa = 4.41KK817 pKa = 10.35KK818 pKa = 11.04ALVAKK823 pKa = 10.51ALGYY827 pKa = 10.47LATDD831 pKa = 3.74TNTPLISHH839 pKa = 7.16WSRR842 pKa = 11.84AIIRR846 pKa = 11.84ACGDD850 pKa = 3.4LQVQPEE856 pKa = 4.42SWWARR861 pKa = 11.84MANPDD866 pKa = 3.33EE867 pKa = 5.27VVFQTSDD874 pKa = 3.1SDD876 pKa = 3.69EE877 pKa = 4.49AKK879 pKa = 10.68ALAAEE884 pKa = 4.72GCGIQVSEE892 pKa = 4.9LDD894 pKa = 4.62EE895 pKa = 4.83ICDD898 pKa = 5.85IIDD901 pKa = 3.28GWNDD905 pKa = 3.01LDD907 pKa = 4.7QFEE910 pKa = 5.47LLLDD914 pKa = 4.22LDD916 pKa = 5.05AEE918 pKa = 4.55VKK920 pKa = 10.81YY921 pKa = 10.25PVVLDD926 pKa = 3.82GSVVGEE932 pKa = 4.15VEE934 pKa = 4.2EE935 pKa = 4.58TVSITSDD942 pKa = 2.89NTSRR946 pKa = 11.84DD947 pKa = 3.46SEE949 pKa = 4.11ISSTRR954 pKa = 11.84DD955 pKa = 3.12SLKK958 pKa = 9.62TSQSAPVRR966 pKa = 11.84RR967 pKa = 11.84RR968 pKa = 11.84PSRR971 pKa = 11.84RR972 pKa = 11.84KK973 pKa = 8.69QSNPVRR979 pKa = 11.84SDD981 pKa = 3.07TTVGDD986 pKa = 3.71RR987 pKa = 11.84VVIASTKK994 pKa = 10.2VEE996 pKa = 4.2PTALGAGPAEE1006 pKa = 4.27PVNSAKK1012 pKa = 10.49LVEE1015 pKa = 4.09RR1016 pKa = 11.84AKK1018 pKa = 10.84FSSGPEE1024 pKa = 3.64

Molecular weight: 115.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KFY0|A0A1L3KFY0_9VIRU RNA replicase OS=Beihai noda-like virus 5 OX=1922487 PE=3 SV=1
MM1 pKa = 6.86KK2 pKa = 9.17TCPVCGRR9 pKa = 11.84KK10 pKa = 9.2FKK12 pKa = 9.8TAQALRR18 pKa = 11.84QHH20 pKa = 6.8RR21 pKa = 11.84AAAHH25 pKa = 6.24RR26 pKa = 11.84GGSASKK32 pKa = 10.14PVLQGGRR39 pKa = 11.84PFPANPSTPTQQFSSEE55 pKa = 3.79NSIVLSGTDD64 pKa = 3.76RR65 pKa = 11.84LYY67 pKa = 10.6MAAVDD72 pKa = 4.92AKK74 pKa = 9.81DD75 pKa = 3.54TPLVRR80 pKa = 11.84LLIGPSLFPRR90 pKa = 11.84LAHH93 pKa = 5.75VAKK96 pKa = 9.17TFQRR100 pKa = 11.84IDD102 pKa = 3.33YY103 pKa = 10.62LMLKK107 pKa = 10.11FEE109 pKa = 4.24IVSRR113 pKa = 11.84FATITPGGYY122 pKa = 8.34VACVFTDD129 pKa = 3.91PDD131 pKa = 4.16DD132 pKa = 5.0APPNDD137 pKa = 3.92PSTLEE142 pKa = 4.89RR143 pKa = 11.84ITSTAGSVQANWWQTSTITAPPKK166 pKa = 9.38YY167 pKa = 10.06GLYY170 pKa = 9.77TSRR173 pKa = 11.84SPTSDD178 pKa = 3.3LRR180 pKa = 11.84FSSPGVFALMTEE192 pKa = 4.44IPPAQGGGFTIMCHH206 pKa = 4.38WKK208 pKa = 10.13ARR210 pKa = 11.84LYY212 pKa = 10.91HH213 pKa = 6.04PTLEE217 pKa = 4.49IDD219 pKa = 3.67VPSPFTSLIATNHH232 pKa = 4.95IKK234 pKa = 10.63AEE236 pKa = 4.1KK237 pKa = 9.12GQPRR241 pKa = 11.84YY242 pKa = 9.58AAEE245 pKa = 3.91SSEE248 pKa = 4.5DD249 pKa = 3.35NRR251 pKa = 11.84FSPEE255 pKa = 3.6VFLDD259 pKa = 3.84GLPSEE264 pKa = 5.22LADD267 pKa = 3.42GDD269 pKa = 4.09YY270 pKa = 11.5DD271 pKa = 4.55FVPEE275 pKa = 3.99TLGVFGYY282 pKa = 10.51RR283 pKa = 11.84AGTGDD288 pKa = 3.84EE289 pKa = 4.17LAAPIARR296 pKa = 11.84VRR298 pKa = 11.84LHH300 pKa = 4.99WRR302 pKa = 11.84GRR304 pKa = 11.84ANPNSNLAPWSPFSGNYY321 pKa = 9.73DD322 pKa = 3.46NPSTSDD328 pKa = 2.91NSTISNIVSWPRR340 pKa = 11.84GTRR343 pKa = 11.84FTLLQSSS350 pKa = 3.98

Molecular weight: 38.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1374

350

1024

687.0

76.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.933 ± 0.627

1.82 ± 0.351

6.041 ± 0.466

5.386 ± 1.015

4.876 ± 0.138

5.968 ± 0.609

1.965 ± 0.018

4.585 ± 0.155

4.294 ± 0.597

7.642 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.82 ± 0.203

3.785 ± 0.036

6.259 ± 1.643

3.202 ± 0.031

6.696 ± 0.064

8.661 ± 0.546

5.75 ± 1.315

8.297 ± 1.784

1.601 ± 0.059

3.421 ± 0.292
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski