Streptomyces phage Vash

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vashvirus; Streptomyces virus Vash

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A411AYW1|A0A411AYW1_9CAUD Thioredoxin OS=Streptomyces phage Vash OX=2510568 GN=43 PE=4 SV=1
MM1 pKa = 7.68DD2 pKa = 4.44TVTLTNAEE10 pKa = 4.2KK11 pKa = 10.58NQTAEE16 pKa = 4.0VVLNGEE22 pKa = 4.31TLTVRR27 pKa = 11.84VLLGSGDD34 pKa = 4.39EE35 pKa = 4.2LTGWTFDD42 pKa = 3.59YY43 pKa = 10.93AADD46 pKa = 4.01GFEE49 pKa = 4.48PGYY52 pKa = 10.88AADD55 pKa = 3.57AAAYY59 pKa = 7.19EE60 pKa = 4.25VSRR63 pKa = 11.84FEE65 pKa = 4.83RR66 pKa = 11.84IGYY69 pKa = 7.72IQNN72 pKa = 3.27

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A411AYT8|A0A411AYT8_9CAUD Major tail protein OS=Streptomyces phage Vash OX=2510568 GN=8 PE=4 SV=1
MM1 pKa = 7.33AQRR4 pKa = 11.84SAYY7 pKa = 8.27TIRR10 pKa = 11.84VDD12 pKa = 4.55GLRR15 pKa = 11.84EE16 pKa = 3.69LQRR19 pKa = 11.84NVRR22 pKa = 11.84TLKK25 pKa = 10.9DD26 pKa = 3.25KK27 pKa = 10.66EE28 pKa = 4.03LNKK31 pKa = 10.37AVRR34 pKa = 11.84EE35 pKa = 4.13ANKK38 pKa = 10.46ASGEE42 pKa = 3.98ILIPQAKK49 pKa = 9.88HH50 pKa = 5.64EE51 pKa = 4.58SPDD54 pKa = 3.22GKK56 pKa = 10.43RR57 pKa = 11.84DD58 pKa = 3.46AKK60 pKa = 10.69SSKK63 pKa = 10.0KK64 pKa = 9.86YY65 pKa = 10.53RR66 pKa = 11.84PGKK69 pKa = 9.35LDD71 pKa = 3.24KK72 pKa = 10.64SIKK75 pKa = 8.76VTASTKK81 pKa = 10.1GAVIKK86 pKa = 10.53AGSAARR92 pKa = 11.84VPYY95 pKa = 10.17AAAIHH100 pKa = 5.81FGYY103 pKa = 10.25RR104 pKa = 11.84KK105 pKa = 10.13RR106 pKa = 11.84NIKK109 pKa = 9.82PNRR112 pKa = 11.84FLFRR116 pKa = 11.84AMARR120 pKa = 11.84KK121 pKa = 8.83SAQVAATYY129 pKa = 8.47EE130 pKa = 3.88RR131 pKa = 11.84RR132 pKa = 11.84IDD134 pKa = 3.71AVVRR138 pKa = 11.84TYY140 pKa = 11.26LEE142 pKa = 4.14SNRR145 pKa = 11.84AGG147 pKa = 3.29

Molecular weight: 16.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12439

45

806

230.4

24.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.477 ± 0.499

0.86 ± 0.131

6.994 ± 0.271

5.804 ± 0.345

3.184 ± 0.155

8.554 ± 0.398

1.712 ± 0.176

3.553 ± 0.266

4.566 ± 0.334

8.055 ± 0.252

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.163 ± 0.134

2.902 ± 0.161

5.338 ± 0.353

2.428 ± 0.201

6.576 ± 0.429

5.627 ± 0.261

6.986 ± 0.467

7.846 ± 0.294

1.889 ± 0.131

2.484 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski