Amino acid dipepetide frequency for Streptomyces phage Vash

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.999AlaAla: 15.999 ± 1.591
1.045AlaCys: 1.045 ± 0.395
10.693AlaAsp: 10.693 ± 1.225
7.879AlaGlu: 7.879 ± 1.018
2.894AlaPhe: 2.894 ± 0.467
8.844AlaGly: 8.844 ± 0.944
2.09AlaHis: 2.09 ± 0.391
4.502AlaIle: 4.502 ± 0.708
5.065AlaLys: 5.065 ± 0.866
11.497AlaLeu: 11.497 ± 0.977
3.296AlaMet: 3.296 ± 0.537
3.698AlaAsn: 3.698 ± 0.494
5.467AlaPro: 5.467 ± 0.762
4.824AlaGln: 4.824 ± 0.579
8.04AlaArg: 8.04 ± 0.937
6.432AlaSer: 6.432 ± 0.91
7.718AlaThr: 7.718 ± 0.869
9.326AlaVal: 9.326 ± 0.807
2.412AlaTrp: 2.412 ± 0.413
3.055AlaTyr: 3.055 ± 0.42
0.0AlaXaa: 0.0 ± 0.0
Cys
0.965CysAla: 0.965 ± 0.345
0.0CysCys: 0.0 ± 0.0
0.884CysAsp: 0.884 ± 0.26
0.563CysGlu: 0.563 ± 0.174
0.241CysPhe: 0.241 ± 0.152
0.804CysGly: 0.804 ± 0.248
0.241CysHis: 0.241 ± 0.128
0.322CysIle: 0.322 ± 0.167
0.161CysLys: 0.161 ± 0.103
0.563CysLeu: 0.563 ± 0.224
0.161CysMet: 0.161 ± 0.102
0.402CysAsn: 0.402 ± 0.184
0.724CysPro: 0.724 ± 0.27
0.241CysGln: 0.241 ± 0.15
0.402CysArg: 0.402 ± 0.165
0.241CysSer: 0.241 ± 0.145
0.322CysThr: 0.322 ± 0.173
0.884CysVal: 0.884 ± 0.266
0.241CysTrp: 0.241 ± 0.18
0.241CysTyr: 0.241 ± 0.135
0.0CysXaa: 0.0 ± 0.0
Asp
12.06AspAla: 12.06 ± 1.324
0.241AspCys: 0.241 ± 0.113
3.779AspAsp: 3.779 ± 0.76
4.02AspGlu: 4.02 ± 0.564
2.653AspPhe: 2.653 ± 0.498
6.995AspGly: 6.995 ± 0.695
1.286AspHis: 1.286 ± 0.246
1.367AspIle: 1.367 ± 0.403
2.332AspLys: 2.332 ± 0.363
5.789AspLeu: 5.789 ± 0.755
1.93AspMet: 1.93 ± 0.351
1.608AspAsn: 1.608 ± 0.37
4.02AspPro: 4.02 ± 0.591
0.965AspGln: 0.965 ± 0.368
3.377AspArg: 3.377 ± 0.537
2.653AspSer: 2.653 ± 0.282
4.422AspThr: 4.422 ± 0.53
7.075AspVal: 7.075 ± 1.0
1.849AspTrp: 1.849 ± 0.351
1.528AspTyr: 1.528 ± 0.337
0.0AspXaa: 0.0 ± 0.0
Glu
8.442GluAla: 8.442 ± 0.962
0.643GluCys: 0.643 ± 0.266
3.055GluAsp: 3.055 ± 0.433
3.538GluGlu: 3.538 ± 0.619
3.055GluPhe: 3.055 ± 0.496
3.538GluGly: 3.538 ± 0.69
1.126GluHis: 1.126 ± 0.277
2.814GluIle: 2.814 ± 0.537
2.412GluLys: 2.412 ± 0.541
5.708GluLeu: 5.708 ± 1.016
0.965GluMet: 0.965 ± 0.343
1.045GluAsn: 1.045 ± 0.305
3.296GluPro: 3.296 ± 0.803
1.126GluGln: 1.126 ± 0.312
4.181GluArg: 4.181 ± 0.589
3.457GluSer: 3.457 ± 0.54
3.618GluThr: 3.618 ± 0.63
3.136GluVal: 3.136 ± 0.627
1.286GluTrp: 1.286 ± 0.263
1.608GluTyr: 1.608 ± 0.423
0.0GluXaa: 0.0 ± 0.0
Phe
4.583PheAla: 4.583 ± 0.713
0.241PheCys: 0.241 ± 0.144
2.653PheAsp: 2.653 ± 0.445
1.045PheGlu: 1.045 ± 0.299
0.884PhePhe: 0.884 ± 0.226
2.412PheGly: 2.412 ± 0.471
0.161PheHis: 0.161 ± 0.117
0.965PheIle: 0.965 ± 0.309
1.688PheLys: 1.688 ± 0.309
3.055PheLeu: 3.055 ± 0.421
0.482PheMet: 0.482 ± 0.184
1.286PheAsn: 1.286 ± 0.326
2.01PhePro: 2.01 ± 0.418
0.724PheGln: 0.724 ± 0.185
1.769PheArg: 1.769 ± 0.359
2.171PheSer: 2.171 ± 0.482
2.814PheThr: 2.814 ± 0.413
2.01PheVal: 2.01 ± 0.357
0.241PheTrp: 0.241 ± 0.134
0.643PheTyr: 0.643 ± 0.189
0.0PheXaa: 0.0 ± 0.0
Gly
10.452GlyAla: 10.452 ± 1.145
0.965GlyCys: 0.965 ± 0.256
4.744GlyAsp: 4.744 ± 0.556
5.065GlyGlu: 5.065 ± 0.581
3.538GlyPhe: 3.538 ± 0.541
6.673GlyGly: 6.673 ± 0.929
1.126GlyHis: 1.126 ± 0.279
2.734GlyIle: 2.734 ± 0.575
4.1GlyLys: 4.1 ± 0.521
6.753GlyLeu: 6.753 ± 1.174
2.171GlyMet: 2.171 ± 0.475
2.814GlyAsn: 2.814 ± 0.482
4.342GlyPro: 4.342 ± 0.667
2.573GlyGln: 2.573 ± 0.491
5.467GlyArg: 5.467 ± 0.562
5.065GlySer: 5.065 ± 0.713
5.708GlyThr: 5.708 ± 0.944
6.191GlyVal: 6.191 ± 1.142
2.01GlyTrp: 2.01 ± 0.458
2.975GlyTyr: 2.975 ± 0.504
0.0GlyXaa: 0.0 ± 0.0
His
1.769HisAla: 1.769 ± 0.492
0.241HisCys: 0.241 ± 0.13
1.286HisAsp: 1.286 ± 0.284
1.447HisGlu: 1.447 ± 0.369
0.322HisPhe: 0.322 ± 0.172
1.206HisGly: 1.206 ± 0.313
0.161HisHis: 0.161 ± 0.119
1.045HisIle: 1.045 ± 0.226
0.965HisLys: 0.965 ± 0.297
1.528HisLeu: 1.528 ± 0.39
0.643HisMet: 0.643 ± 0.289
0.241HisAsn: 0.241 ± 0.137
1.126HisPro: 1.126 ± 0.317
0.241HisGln: 0.241 ± 0.199
0.884HisArg: 0.884 ± 0.195
1.126HisSer: 1.126 ± 0.22
0.482HisThr: 0.482 ± 0.204
1.608HisVal: 1.608 ± 0.36
0.563HisTrp: 0.563 ± 0.218
0.241HisTyr: 0.241 ± 0.124
0.0HisXaa: 0.0 ± 0.0
Ile
3.618IleAla: 3.618 ± 0.675
0.322IleCys: 0.322 ± 0.164
3.698IleAsp: 3.698 ± 0.47
2.332IleGlu: 2.332 ± 0.475
0.724IlePhe: 0.724 ± 0.288
3.136IleGly: 3.136 ± 0.412
0.884IleHis: 0.884 ± 0.394
1.045IleIle: 1.045 ± 0.292
2.01IleLys: 2.01 ± 0.39
2.09IleLeu: 2.09 ± 0.35
0.724IleMet: 0.724 ± 0.209
0.965IleAsn: 0.965 ± 0.29
2.251IlePro: 2.251 ± 0.39
1.045IleGln: 1.045 ± 0.344
2.814IleArg: 2.814 ± 0.567
1.528IleSer: 1.528 ± 0.372
2.492IleThr: 2.492 ± 0.373
2.573IleVal: 2.573 ± 0.366
0.482IleTrp: 0.482 ± 0.189
0.804IleTyr: 0.804 ± 0.275
0.0IleXaa: 0.0 ± 0.0
Lys
6.03LysAla: 6.03 ± 1.114
0.563LysCys: 0.563 ± 0.266
2.573LysAsp: 2.573 ± 0.406
2.171LysGlu: 2.171 ± 0.347
1.045LysPhe: 1.045 ± 0.302
3.538LysGly: 3.538 ± 0.51
1.206LysHis: 1.206 ± 0.238
1.528LysIle: 1.528 ± 0.304
2.09LysLys: 2.09 ± 0.461
3.457LysLeu: 3.457 ± 0.555
1.206LysMet: 1.206 ± 0.335
1.286LysAsn: 1.286 ± 0.364
3.216LysPro: 3.216 ± 0.61
0.241LysGln: 0.241 ± 0.127
4.261LysArg: 4.261 ± 0.696
2.332LysSer: 2.332 ± 0.502
3.055LysThr: 3.055 ± 0.794
3.859LysVal: 3.859 ± 0.491
0.724LysTrp: 0.724 ± 0.295
1.286LysTyr: 1.286 ± 0.325
0.0LysXaa: 0.0 ± 0.0
Leu
7.397LeuAla: 7.397 ± 0.932
0.724LeuCys: 0.724 ± 0.232
6.914LeuAsp: 6.914 ± 0.778
4.181LeuGlu: 4.181 ± 0.738
2.573LeuPhe: 2.573 ± 0.342
5.789LeuGly: 5.789 ± 0.675
0.884LeuHis: 0.884 ± 0.253
3.859LeuIle: 3.859 ± 0.518
4.904LeuLys: 4.904 ± 0.562
5.628LeuLeu: 5.628 ± 0.914
1.126LeuMet: 1.126 ± 0.32
2.975LeuAsn: 2.975 ± 0.48
5.306LeuPro: 5.306 ± 0.722
1.688LeuGln: 1.688 ± 0.407
6.191LeuArg: 6.191 ± 0.968
6.593LeuSer: 6.593 ± 0.885
6.753LeuThr: 6.753 ± 0.773
5.065LeuVal: 5.065 ± 0.779
0.884LeuTrp: 0.884 ± 0.236
1.126LeuTyr: 1.126 ± 0.282
0.0LeuXaa: 0.0 ± 0.0
Met
3.136MetAla: 3.136 ± 0.564
0.161MetCys: 0.161 ± 0.108
0.884MetAsp: 0.884 ± 0.264
0.402MetGlu: 0.402 ± 0.166
0.322MetPhe: 0.322 ± 0.155
2.894MetGly: 2.894 ± 0.577
0.241MetHis: 0.241 ± 0.133
0.804MetIle: 0.804 ± 0.262
1.286MetLys: 1.286 ± 0.292
1.849MetLeu: 1.849 ± 0.316
0.241MetMet: 0.241 ± 0.146
0.402MetAsn: 0.402 ± 0.161
1.849MetPro: 1.849 ± 0.384
0.322MetGln: 0.322 ± 0.134
1.608MetArg: 1.608 ± 0.393
1.367MetSer: 1.367 ± 0.309
1.447MetThr: 1.447 ± 0.249
1.849MetVal: 1.849 ± 0.378
0.322MetTrp: 0.322 ± 0.147
0.241MetTyr: 0.241 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
4.342AsnAla: 4.342 ± 0.551
0.08AsnCys: 0.08 ± 0.095
1.769AsnAsp: 1.769 ± 0.374
1.608AsnGlu: 1.608 ± 0.404
0.724AsnPhe: 0.724 ± 0.209
3.94AsnGly: 3.94 ± 0.458
0.563AsnHis: 0.563 ± 0.18
0.643AsnIle: 0.643 ± 0.278
1.528AsnLys: 1.528 ± 0.362
1.769AsnLeu: 1.769 ± 0.306
0.563AsnMet: 0.563 ± 0.235
0.724AsnAsn: 0.724 ± 0.255
2.412AsnPro: 2.412 ± 0.402
0.965AsnGln: 0.965 ± 0.278
0.965AsnArg: 0.965 ± 0.414
1.447AsnSer: 1.447 ± 0.347
1.608AsnThr: 1.608 ± 0.256
2.251AsnVal: 2.251 ± 0.358
0.482AsnTrp: 0.482 ± 0.23
0.643AsnTyr: 0.643 ± 0.201
0.0AsnXaa: 0.0 ± 0.0
Pro
6.432ProAla: 6.432 ± 0.734
0.724ProCys: 0.724 ± 0.298
4.985ProAsp: 4.985 ± 0.712
4.502ProGlu: 4.502 ± 0.729
2.09ProPhe: 2.09 ± 0.393
5.548ProGly: 5.548 ± 0.838
1.126ProHis: 1.126 ± 0.347
1.206ProIle: 1.206 ± 0.308
2.814ProLys: 2.814 ± 0.519
2.01ProLeu: 2.01 ± 0.457
1.206ProMet: 1.206 ± 0.251
2.171ProAsn: 2.171 ± 0.432
1.206ProPro: 1.206 ± 0.363
0.724ProGln: 0.724 ± 0.26
2.412ProArg: 2.412 ± 0.426
3.457ProSer: 3.457 ± 0.69
4.261ProThr: 4.261 ± 0.615
4.342ProVal: 4.342 ± 0.793
0.804ProTrp: 0.804 ± 0.285
1.367ProTyr: 1.367 ± 0.306
0.0ProXaa: 0.0 ± 0.0
Gln
2.734GlnAla: 2.734 ± 0.462
0.241GlnCys: 0.241 ± 0.131
1.688GlnAsp: 1.688 ± 0.352
0.563GlnGlu: 0.563 ± 0.209
1.367GlnPhe: 1.367 ± 0.382
2.332GlnGly: 2.332 ± 0.375
0.643GlnHis: 0.643 ± 0.323
0.724GlnIle: 0.724 ± 0.257
0.643GlnLys: 0.643 ± 0.25
1.608GlnLeu: 1.608 ± 0.385
0.482GlnMet: 0.482 ± 0.182
0.884GlnAsn: 0.884 ± 0.272
1.286GlnPro: 1.286 ± 0.358
0.402GlnGln: 0.402 ± 0.202
2.573GlnArg: 2.573 ± 0.508
0.965GlnSer: 0.965 ± 0.227
2.09GlnThr: 2.09 ± 0.523
1.93GlnVal: 1.93 ± 0.408
0.643GlnTrp: 0.643 ± 0.267
0.482GlnTyr: 0.482 ± 0.195
0.0GlnXaa: 0.0 ± 0.0
Arg
8.281ArgAla: 8.281 ± 0.796
0.482ArgCys: 0.482 ± 0.193
4.583ArgAsp: 4.583 ± 0.657
4.824ArgGlu: 4.824 ± 0.657
2.573ArgPhe: 2.573 ± 0.432
3.859ArgGly: 3.859 ± 0.675
1.688ArgHis: 1.688 ± 0.409
3.296ArgIle: 3.296 ± 0.468
4.1ArgLys: 4.1 ± 0.579
5.065ArgLeu: 5.065 ± 0.793
1.608ArgMet: 1.608 ± 0.406
1.849ArgAsn: 1.849 ± 0.337
1.849ArgPro: 1.849 ± 0.325
1.528ArgGln: 1.528 ± 0.377
4.02ArgArg: 4.02 ± 0.603
4.181ArgSer: 4.181 ± 0.65
3.136ArgThr: 3.136 ± 0.484
5.387ArgVal: 5.387 ± 0.585
1.528ArgTrp: 1.528 ± 0.333
1.93ArgTyr: 1.93 ± 0.397
0.0ArgXaa: 0.0 ± 0.0
Ser
6.753SerAla: 6.753 ± 0.657
0.482SerCys: 0.482 ± 0.237
4.181SerAsp: 4.181 ± 0.642
3.055SerGlu: 3.055 ± 0.498
1.206SerPhe: 1.206 ± 0.26
4.985SerGly: 4.985 ± 0.756
0.965SerHis: 0.965 ± 0.317
2.412SerIle: 2.412 ± 0.289
2.332SerLys: 2.332 ± 0.515
5.467SerLeu: 5.467 ± 0.535
1.126SerMet: 1.126 ± 0.356
1.608SerAsn: 1.608 ± 0.47
2.814SerPro: 2.814 ± 0.535
1.447SerGln: 1.447 ± 0.304
2.734SerArg: 2.734 ± 0.535
3.216SerSer: 3.216 ± 0.527
4.02SerThr: 4.02 ± 0.651
4.904SerVal: 4.904 ± 0.529
1.206SerTrp: 1.206 ± 0.258
1.367SerTyr: 1.367 ± 0.378
0.0SerXaa: 0.0 ± 0.0
Thr
7.718ThrAla: 7.718 ± 0.595
0.482ThrCys: 0.482 ± 0.26
4.02ThrAsp: 4.02 ± 0.947
3.618ThrGlu: 3.618 ± 0.469
2.251ThrPhe: 2.251 ± 0.371
8.201ThrGly: 8.201 ± 1.209
0.724ThrHis: 0.724 ± 0.244
2.09ThrIle: 2.09 ± 0.363
2.734ThrLys: 2.734 ± 0.455
6.995ThrLeu: 6.995 ± 0.791
1.045ThrMet: 1.045 ± 0.284
1.93ThrAsn: 1.93 ± 0.351
3.779ThrPro: 3.779 ± 0.63
2.09ThrGln: 2.09 ± 0.409
3.779ThrArg: 3.779 ± 0.555
3.055ThrSer: 3.055 ± 0.449
6.352ThrThr: 6.352 ± 0.879
5.467ThrVal: 5.467 ± 0.722
1.126ThrTrp: 1.126 ± 0.291
2.412ThrTyr: 2.412 ± 0.491
0.0ThrXaa: 0.0 ± 0.0
Val
8.844ValAla: 8.844 ± 1.429
0.482ValCys: 0.482 ± 0.228
4.261ValAsp: 4.261 ± 0.566
4.342ValGlu: 4.342 ± 0.862
2.412ValPhe: 2.412 ± 0.432
6.191ValGly: 6.191 ± 0.729
1.126ValHis: 1.126 ± 0.303
3.296ValIle: 3.296 ± 0.45
2.894ValLys: 2.894 ± 0.653
6.352ValLeu: 6.352 ± 0.694
1.608ValMet: 1.608 ± 0.286
2.332ValAsn: 2.332 ± 0.368
4.824ValPro: 4.824 ± 0.813
1.93ValGln: 1.93 ± 0.44
6.191ValArg: 6.191 ± 0.738
4.342ValSer: 4.342 ± 0.629
6.834ValThr: 6.834 ± 0.729
6.352ValVal: 6.352 ± 0.855
1.769ValTrp: 1.769 ± 0.4
2.09ValTyr: 2.09 ± 0.343
0.0ValXaa: 0.0 ± 0.0
Trp
1.849TrpAla: 1.849 ± 0.356
0.241TrpCys: 0.241 ± 0.125
1.045TrpAsp: 1.045 ± 0.26
1.045TrpGlu: 1.045 ± 0.284
0.643TrpPhe: 0.643 ± 0.294
1.608TrpGly: 1.608 ± 0.375
0.482TrpHis: 0.482 ± 0.177
0.724TrpIle: 0.724 ± 0.249
0.643TrpLys: 0.643 ± 0.223
1.93TrpLeu: 1.93 ± 0.403
0.322TrpMet: 0.322 ± 0.139
0.402TrpAsn: 0.402 ± 0.19
0.402TrpPro: 0.402 ± 0.166
0.724TrpGln: 0.724 ± 0.21
1.769TrpArg: 1.769 ± 0.446
1.126TrpSer: 1.126 ± 0.243
1.608TrpThr: 1.608 ± 0.486
1.849TrpVal: 1.849 ± 0.39
0.08TrpTrp: 0.08 ± 0.082
0.402TrpTyr: 0.402 ± 0.194
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.377TyrAla: 3.377 ± 0.534
0.241TyrCys: 0.241 ± 0.148
2.251TyrAsp: 2.251 ± 0.357
1.849TyrGlu: 1.849 ± 0.41
0.322TyrPhe: 0.322 ± 0.176
3.055TyrGly: 3.055 ± 0.462
0.402TyrHis: 0.402 ± 0.166
0.161TyrIle: 0.161 ± 0.125
0.965TyrLys: 0.965 ± 0.277
1.447TyrLeu: 1.447 ± 0.3
0.643TyrMet: 0.643 ± 0.294
0.402TyrAsn: 0.402 ± 0.182
1.206TyrPro: 1.206 ± 0.283
0.482TyrGln: 0.482 ± 0.147
2.332TyrArg: 2.332 ± 0.525
1.528TyrSer: 1.528 ± 0.396
1.126TyrThr: 1.126 ± 0.255
2.412TyrVal: 2.412 ± 0.424
0.241TyrTrp: 0.241 ± 0.13
0.402TyrTyr: 0.402 ± 0.168
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (12439 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski