Halomonas sp. BC04

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas; unclassified Halomonas

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6235 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W7QA22|W7QA22_9GAMM Uncharacterized protein (Fragment) OS=Halomonas sp. BC04 OX=1403540 GN=Q427_31400 PE=4 SV=1
AA1 pKa = 7.85ANNDD5 pKa = 3.19SDD7 pKa = 4.59VNQTGYY13 pKa = 11.27YY14 pKa = 10.02NNADD18 pKa = 3.34VFQTGNNHH26 pKa = 6.14EE27 pKa = 4.59SNITQDD33 pKa = 4.04GIGNDD38 pKa = 3.49SDD40 pKa = 3.92VAQYY44 pKa = 10.94GYY46 pKa = 10.18SQSSDD51 pKa = 2.59IDD53 pKa = 3.29QDD55 pKa = 3.06GWGNEE60 pKa = 4.07GVVVQHH66 pKa = 5.91GWGNDD71 pKa = 3.13SSIDD75 pKa = 3.35QTGYY79 pKa = 10.96FNDD82 pKa = 3.32ADD84 pKa = 4.29TYY86 pKa = 11.14QKK88 pKa = 10.4GTGNEE93 pKa = 4.1VTHH96 pKa = 6.67LQAGWGNSSEE106 pKa = 4.43SVQKK110 pKa = 10.82GWGNTASIDD119 pKa = 3.65QDD121 pKa = 3.71GKK123 pKa = 10.46NLSSDD128 pKa = 3.71VYY130 pKa = 9.3QTGRR134 pKa = 11.84RR135 pKa = 11.84NDD137 pKa = 3.52ADD139 pKa = 3.66VTQTGVSNSSTIVQEE154 pKa = 4.47GKK156 pKa = 10.17FWGQNSATVNQSGFKK171 pKa = 9.85NDD173 pKa = 3.5SDD175 pKa = 3.22ISQYY179 pKa = 11.68GDD181 pKa = 3.35FNTASTTQSGVNNDD195 pKa = 3.09AVVVQKK201 pKa = 10.59GWNNDD206 pKa = 2.86STINQSGYY214 pKa = 10.74NDD216 pKa = 4.07LADD219 pKa = 3.63VYY221 pKa = 11.41QNGKK225 pKa = 8.52NHH227 pKa = 6.72TSTVTQSGAGWGAFAQNTATVVQTGQNNMSNVAQNGGGNTATVTQQQ273 pKa = 2.65

Molecular weight: 28.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W7PRA1|W7PRA1_9GAMM Methyltranfer_dom domain-containing protein OS=Halomonas sp. BC04 OX=1403540 GN=Q427_32910 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6235

0

6235

1595998

29

2848

256.0

28.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.817 ± 0.038

1.003 ± 0.011

5.633 ± 0.031

6.622 ± 0.038

3.494 ± 0.021

8.171 ± 0.032

2.52 ± 0.016

4.805 ± 0.03

2.551 ± 0.025

11.451 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.018

2.487 ± 0.018

5.039 ± 0.025

3.772 ± 0.021

7.469 ± 0.038

5.588 ± 0.021

4.939 ± 0.021

7.073 ± 0.026

1.548 ± 0.017

2.35 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski