Sphaerobolus stellatus (strain SS14)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Phallomycetidae; Geastrales; Sphaerobolaceae; Sphaerobolus; Sphaerobolus stellatus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24609 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C9UX48|A0A0C9UX48_SPHS4 Unplaced genomic scaffold SPHSTscaffold_132 whole genome shotgun sequence OS=Sphaerobolus stellatus (strain SS14) OX=990650 GN=M422DRAFT_52261 PE=4 SV=1
MM1 pKa = 7.78PGGVHH6 pKa = 6.36TGDD9 pKa = 3.43LTWYY13 pKa = 9.81HH14 pKa = 6.93GEE16 pKa = 3.94LTACGDD22 pKa = 3.65TTTDD26 pKa = 2.68SDD28 pKa = 3.89FGVALGAALFDD39 pKa = 4.31SLNTDD44 pKa = 3.41GDD46 pKa = 4.46SNDD49 pKa = 3.71NAACFQKK56 pKa = 10.62IMITANGKK64 pKa = 5.87TAYY67 pKa = 8.85GTVDD71 pKa = 4.19DD72 pKa = 4.53RR73 pKa = 11.84CEE75 pKa = 4.23GCAEE79 pKa = 3.85WDD81 pKa = 3.37IDD83 pKa = 3.82VLPALFEE90 pKa = 4.18EE91 pKa = 5.07FAPLSAGRR99 pKa = 11.84IHH101 pKa = 7.36DD102 pKa = 4.02VQWQFVAGNN111 pKa = 3.56

Molecular weight: 11.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C9UKN7|A0A0C9UKN7_SPHS4 Unplaced genomic scaffold SPHSTscaffold_313 whole genome shotgun sequence OS=Sphaerobolus stellatus (strain SS14) OX=990650 GN=M422DRAFT_236724 PE=3 SV=1
AA1 pKa = 7.4KK2 pKa = 9.91HH3 pKa = 5.82IRR5 pKa = 11.84LRR7 pKa = 11.84PPIPTLPILPQNKK20 pKa = 9.27HH21 pKa = 5.4NLLILLLLPIPLPLLHH37 pKa = 6.96LSSLLHH43 pKa = 6.55LPLPLITPPMHH54 pKa = 6.2MLRR57 pKa = 11.84RR58 pKa = 11.84AHH60 pKa = 6.36HH61 pKa = 5.26PHH63 pKa = 6.16RR64 pKa = 11.84HH65 pKa = 5.11KK66 pKa = 11.09LMPRR70 pKa = 11.84MPRR73 pKa = 11.84RR74 pKa = 11.84CWWRR78 pKa = 11.84SPMM81 pKa = 4.51

Molecular weight: 9.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24605

4

24609

7189526

49

3671

292.2

32.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.656 ± 0.016

1.439 ± 0.007

5.396 ± 0.013

6.07 ± 0.018

3.918 ± 0.012

6.171 ± 0.017

2.726 ± 0.01

5.655 ± 0.014

4.992 ± 0.016

9.331 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.007

4.018 ± 0.01

6.161 ± 0.018

3.965 ± 0.01

5.633 ± 0.016

8.248 ± 0.02

6.106 ± 0.014

5.946 ± 0.014

1.56 ± 0.007

2.814 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski