Amino acid dipepetide frequency for Sphaerobolus stellatus (strain SS14)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.078AlaAla: 7.078 ± 0.046	1.08AlaCys: 1.08 ± 0.013	3.52AlaAsp: 3.52 ± 0.023	4.411AlaGlu: 4.411 ± 0.03	3.164AlaPhe: 3.164 ± 0.025	4.652AlaGly: 4.652 ± 0.028	1.948AlaHis: 1.948 ± 0.016	4.286AlaIle: 4.286 ± 0.027	3.737AlaLys: 3.737 ± 0.028	7.486AlaLeu: 7.486 ± 0.034	1.746AlaMet: 1.746 ± 0.017	2.892AlaAsn: 2.892 ± 0.02	4.165AlaPro: 4.165 ± 0.037	3.038AlaGln: 3.038 ± 0.022	4.179AlaArg: 4.179 ± 0.027	6.343AlaSer: 6.343 ± 0.034	4.704AlaThr: 4.704 ± 0.028	4.826AlaVal: 4.826 ± 0.03	1.152AlaTrp: 1.152 ± 0.015	2.151AlaTyr: 2.151 ± 0.016	0.0AlaXaa: 0.0 ± 0.0
Cys	0.957CysAla: 0.957 ± 0.011	0.311CysCys: 0.311 ± 0.008	0.691CysAsp: 0.691 ± 0.01	0.67CysGlu: 0.67 ± 0.011	0.622CysPhe: 0.622 ± 0.01	0.93CysGly: 0.93 ± 0.013	0.443CysHis: 0.443 ± 0.009	0.863CysIle: 0.863 ± 0.013	0.673CysLys: 0.673 ± 0.009	1.456CysLeu: 1.456 ± 0.015	0.386CysMet: 0.386 ± 0.011	0.58CysAsn: 0.58 ± 0.008	0.905CysPro: 0.905 ± 0.012	0.581CysGln: 0.581 ± 0.01	0.841CysArg: 0.841 ± 0.011	1.155CysSer: 1.155 ± 0.014	0.859CysThr: 0.859 ± 0.013	0.815CysVal: 0.815 ± 0.011	0.232CysTrp: 0.232 ± 0.005	0.418CysTyr: 0.418 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	4.001AspAla: 4.001 ± 0.026	0.724AspCys: 0.724 ± 0.011	3.498AspAsp: 3.498 ± 0.026	4.002AspGlu: 4.002 ± 0.031	2.218AspPhe: 2.218 ± 0.019	3.683AspGly: 3.683 ± 0.025	1.307AspHis: 1.307 ± 0.015	3.791AspIle: 3.791 ± 0.026	2.37AspLys: 2.37 ± 0.02	4.847AspLeu: 4.847 ± 0.031	1.247AspMet: 1.247 ± 0.014	2.087AspAsn: 2.087 ± 0.018	3.312AspPro: 3.312 ± 0.024	1.662AspGln: 1.662 ± 0.017	2.461AspArg: 2.461 ± 0.02	3.943AspSer: 3.943 ± 0.03	3.044AspThr: 3.044 ± 0.02	3.454AspVal: 3.454 ± 0.024	0.845AspTrp: 0.845 ± 0.012	1.463AspTyr: 1.463 ± 0.015	0.0AspXaa: 0.0 ± 0.0
Glu	5.024GluAla: 5.024 ± 0.033	0.728GluCys: 0.728 ± 0.011	4.286GluAsp: 4.286 ± 0.033	5.795GluGlu: 5.795 ± 0.054	2.044GluPhe: 2.044 ± 0.016	3.705GluGly: 3.705 ± 0.031	1.484GluHis: 1.484 ± 0.015	3.194GluIle: 3.194 ± 0.023	3.448GluLys: 3.448 ± 0.029	5.486GluLeu: 5.486 ± 0.032	1.459GluMet: 1.459 ± 0.019	2.259GluAsn: 2.259 ± 0.018	2.549GluPro: 2.549 ± 0.023	2.307GluGln: 2.307 ± 0.018	3.52GluArg: 3.52 ± 0.026	3.888GluSer: 3.888 ± 0.026	3.153GluThr: 3.153 ± 0.022	3.566GluVal: 3.566 ± 0.026	1.025GluTrp: 1.025 ± 0.013	1.776GluTyr: 1.776 ± 0.02	0.0GluXaa: 0.0 ± 0.0
Phe	2.778PheAla: 2.778 ± 0.02	0.59PheCys: 0.59 ± 0.009	2.246PheAsp: 2.246 ± 0.017	2.209PheGlu: 2.209 ± 0.02	1.668PhePhe: 1.668 ± 0.018	2.584PheGly: 2.584 ± 0.019	1.085PheHis: 1.085 ± 0.013	2.302PheIle: 2.302 ± 0.019	1.834PheLys: 1.834 ± 0.014	3.792PheLeu: 3.792 ± 0.025	0.897PheMet: 0.897 ± 0.011	1.75PheAsn: 1.75 ± 0.016	2.24PhePro: 2.24 ± 0.019	1.545PheGln: 1.545 ± 0.019	1.941PheArg: 1.941 ± 0.017	3.242PheSer: 3.242 ± 0.023	2.352PheThr: 2.352 ± 0.02	2.329PheVal: 2.329 ± 0.019	0.635PheTrp: 0.635 ± 0.009	1.159PheTyr: 1.159 ± 0.013	0.0PheXaa: 0.0 ± 0.0
Gly	4.038GlyAla: 4.038 ± 0.024	0.953GlyCys: 0.953 ± 0.014	3.116GlyAsp: 3.116 ± 0.024	3.432GlyGlu: 3.432 ± 0.027	2.515GlyPhe: 2.515 ± 0.022	4.613GlyGly: 4.613 ± 0.037	1.775GlyHis: 1.775 ± 0.022	3.666GlyIle: 3.666 ± 0.023	3.537GlyLys: 3.537 ± 0.027	5.354GlyLeu: 5.354 ± 0.028	1.409GlyMet: 1.409 ± 0.015	2.596GlyAsn: 2.596 ± 0.026	3.018GlyPro: 3.018 ± 0.025	2.341GlyGln: 2.341 ± 0.021	3.58GlyArg: 3.58 ± 0.027	5.185GlySer: 5.185 ± 0.029	3.844GlyThr: 3.844 ± 0.025	3.731GlyVal: 3.731 ± 0.024	1.088GlyTrp: 1.088 ± 0.013	1.916GlyTyr: 1.916 ± 0.019	0.0GlyXaa: 0.0 ± 0.0
His	1.9HisAla: 1.9 ± 0.013	0.506HisCys: 0.506 ± 0.008	1.325HisAsp: 1.325 ± 0.015	1.343HisGlu: 1.343 ± 0.016	1.128HisPhe: 1.128 ± 0.013	1.61HisGly: 1.61 ± 0.014	0.909HisHis: 0.909 ± 0.012	1.774HisIle: 1.774 ± 0.017	1.155HisLys: 1.155 ± 0.015	2.627HisLeu: 2.627 ± 0.02	0.606HisMet: 0.606 ± 0.008	1.067HisAsn: 1.067 ± 0.012	2.106HisPro: 2.106 ± 0.021	1.052HisGln: 1.052 ± 0.013	1.579HisArg: 1.579 ± 0.015	2.135HisSer: 2.135 ± 0.019	1.693HisThr: 1.693 ± 0.024	1.526HisVal: 1.526 ± 0.014	0.433HisTrp: 0.433 ± 0.008	0.784HisTyr: 0.784 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	4.129IleAla: 4.129 ± 0.025	0.934IleCys: 0.934 ± 0.013	2.977IleAsp: 2.977 ± 0.022	3.034IleGlu: 3.034 ± 0.024	2.352IlePhe: 2.352 ± 0.019	3.213IleGly: 3.213 ± 0.023	1.579IleHis: 1.579 ± 0.015	3.367IleIle: 3.367 ± 0.025	2.562IleLys: 2.562 ± 0.021	5.586IleLeu: 5.586 ± 0.031	1.181IleMet: 1.181 ± 0.013	2.252IleAsn: 2.252 ± 0.019	4.017IlePro: 4.017 ± 0.026	2.381IleGln: 2.381 ± 0.022	3.15IleArg: 3.15 ± 0.018	4.58IleSer: 4.58 ± 0.028	3.349IleThr: 3.349 ± 0.026	3.411IleVal: 3.411 ± 0.023	0.952IleTrp: 0.952 ± 0.013	1.546IleTyr: 1.546 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	4.363LysAla: 4.363 ± 0.03	0.675LysCys: 0.675 ± 0.01	2.906LysAsp: 2.906 ± 0.023	3.698LysGlu: 3.698 ± 0.03	1.623LysPhe: 1.623 ± 0.017	3.017LysGly: 3.017 ± 0.025	1.339LysHis: 1.339 ± 0.013	2.445LysIle: 2.445 ± 0.021	3.358LysLys: 3.358 ± 0.033	4.479LysLeu: 4.479 ± 0.028	1.041LysMet: 1.041 ± 0.011	1.845LysAsn: 1.845 ± 0.016	2.58LysPro: 2.58 ± 0.022	1.928LysGln: 1.928 ± 0.019	3.165LysArg: 3.165 ± 0.024	3.325LysSer: 3.325 ± 0.024	2.831LysThr: 2.831 ± 0.021	2.908LysVal: 2.908 ± 0.024	0.893LysTrp: 0.893 ± 0.016	1.502LysTyr: 1.502 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	7.152LeuAla: 7.152 ± 0.038	1.393LeuCys: 1.393 ± 0.014	4.971LeuAsp: 4.971 ± 0.03	5.884LeuGlu: 5.884 ± 0.039	3.528LeuPhe: 3.528 ± 0.027	5.701LeuGly: 5.701 ± 0.036	2.696LeuHis: 2.696 ± 0.023	4.56LeuIle: 4.56 ± 0.029	4.795LeuLys: 4.795 ± 0.025	8.842LeuLeu: 8.842 ± 0.048	1.824LeuMet: 1.824 ± 0.018	3.653LeuAsn: 3.653 ± 0.029	6.246LeuPro: 6.246 ± 0.028	4.213LeuGln: 4.213 ± 0.03	5.617LeuArg: 5.617 ± 0.03	7.678LeuSer: 7.678 ± 0.038	5.472LeuThr: 5.472 ± 0.034	5.157LeuVal: 5.157 ± 0.032	1.371LeuTrp: 1.371 ± 0.016	2.552LeuTyr: 2.552 ± 0.021	0.0LeuXaa: 0.0 ± 0.0
Met	1.804MetAla: 1.804 ± 0.017	0.268MetCys: 0.268 ± 0.006	1.385MetAsp: 1.385 ± 0.015	1.426MetGlu: 1.426 ± 0.016	0.724MetPhe: 0.724 ± 0.009	1.284MetGly: 1.284 ± 0.014	0.619MetHis: 0.619 ± 0.009	1.012MetIle: 1.012 ± 0.013	1.203MetLys: 1.203 ± 0.014	1.898MetLeu: 1.898 ± 0.017	0.502MetMet: 0.502 ± 0.008	0.921MetAsn: 0.921 ± 0.014	1.299MetPro: 1.299 ± 0.014	0.903MetGln: 0.903 ± 0.011	1.297MetArg: 1.297 ± 0.017	2.013MetSer: 2.013 ± 0.019	1.322MetThr: 1.322 ± 0.014	1.309MetVal: 1.309 ± 0.014	0.277MetTrp: 0.277 ± 0.007	0.506MetTyr: 0.506 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	3.288AsnAla: 3.288 ± 0.022	0.558AsnCys: 0.558 ± 0.01	2.049AsnAsp: 2.049 ± 0.017	2.113AsnGlu: 2.113 ± 0.02	1.49AsnPhe: 1.49 ± 0.013	2.723AsnGly: 2.723 ± 0.023	1.01AsnHis: 1.01 ± 0.013	2.7AsnIle: 2.7 ± 0.023	1.731AsnLys: 1.731 ± 0.017	3.768AsnLeu: 3.768 ± 0.03	0.897AsnMet: 0.897 ± 0.011	1.934AsnAsn: 1.934 ± 0.022	2.617AsnPro: 2.617 ± 0.018	1.457AsnGln: 1.457 ± 0.017	1.907AsnArg: 1.907 ± 0.016	3.06AsnSer: 3.06 ± 0.025	2.609AsnThr: 2.609 ± 0.021	2.595AsnVal: 2.595 ± 0.021	0.62AsnTrp: 0.62 ± 0.009	1.051AsnTyr: 1.051 ± 0.01	0.0AsnXaa: 0.0 ± 0.0
Pro	4.676ProAla: 4.676 ± 0.031	0.636ProCys: 0.636 ± 0.009	3.126ProAsp: 3.126 ± 0.025	3.652ProGlu: 3.652 ± 0.026	2.284ProPhe: 2.284 ± 0.02	3.525ProGly: 3.525 ± 0.024	1.683ProHis: 1.683 ± 0.017	3.082ProIle: 3.082 ± 0.022	2.765ProLys: 2.765 ± 0.025	5.183ProLeu: 5.183 ± 0.029	1.101ProMet: 1.101 ± 0.013	2.568ProAsn: 2.568 ± 0.022	5.335ProPro: 5.335 ± 0.052	2.426ProGln: 2.426 ± 0.023	3.152ProArg: 3.152 ± 0.026	6.446ProSer: 6.446 ± 0.041	4.183ProThr: 4.183 ± 0.032	3.436ProVal: 3.436 ± 0.028	0.727ProTrp: 0.727 ± 0.011	1.626ProTyr: 1.626 ± 0.017	0.0ProXaa: 0.0 ± 0.0
Gln	3.329GlnAla: 3.329 ± 0.027	0.64GlnCys: 0.64 ± 0.011	2.038GlnAsp: 2.038 ± 0.021	2.468GlnGlu: 2.468 ± 0.021	1.369GlnPhe: 1.369 ± 0.015	2.453GlnGly: 2.453 ± 0.02	1.094GlnHis: 1.094 ± 0.014	1.994GlnIle: 1.994 ± 0.016	1.848GlnLys: 1.848 ± 0.017	3.796GlnLeu: 3.796 ± 0.024	0.934GlnMet: 0.934 ± 0.012	1.539GlnAsn: 1.539 ± 0.015	2.363GlnPro: 2.363 ± 0.023	2.033GlnGln: 2.033 ± 0.036	2.285GlnArg: 2.285 ± 0.018	3.099GlnSer: 3.099 ± 0.026	2.309GlnThr: 2.309 ± 0.02	2.192GlnVal: 2.192 ± 0.017	0.652GlnTrp: 0.652 ± 0.01	1.216GlnTyr: 1.216 ± 0.015	0.0GlnXaa: 0.0 ± 0.0
Arg	3.976ArgAla: 3.976 ± 0.024	0.835ArgCys: 0.835 ± 0.011	2.822ArgAsp: 2.822 ± 0.023	3.382ArgGlu: 3.382 ± 0.026	2.23ArgPhe: 2.23 ± 0.02	2.999ArgGly: 2.999 ± 0.025	1.579ArgHis: 1.579 ± 0.015	3.278ArgIle: 3.278 ± 0.024	3.326ArgLys: 3.326 ± 0.026	5.329ArgLeu: 5.329 ± 0.031	1.338ArgMet: 1.338 ± 0.015	2.254ArgAsn: 2.254 ± 0.017	3.067ArgPro: 3.067 ± 0.023	2.204ArgGln: 2.204 ± 0.022	4.267ArgArg: 4.267 ± 0.034	4.282ArgSer: 4.282 ± 0.026	3.291ArgThr: 3.291 ± 0.025	3.223ArgVal: 3.223 ± 0.026	0.978ArgTrp: 0.978 ± 0.012	1.671ArgTyr: 1.671 ± 0.017	0.0ArgXaa: 0.0 ± 0.0
Ser	5.648SerAla: 5.648 ± 0.032	1.102SerCys: 1.102 ± 0.015	4.094SerAsp: 4.094 ± 0.028	4.144SerGlu: 4.144 ± 0.03	3.378SerPhe: 3.378 ± 0.025	5.1SerGly: 5.1 ± 0.032	2.235SerHis: 2.235 ± 0.021	4.588SerIle: 4.588 ± 0.029	3.842SerLys: 3.842 ± 0.028	7.744SerLeu: 7.744 ± 0.034	1.732SerMet: 1.732 ± 0.017	3.332SerAsn: 3.332 ± 0.022	5.438SerPro: 5.438 ± 0.044	3.253SerGln: 3.253 ± 0.027	4.663SerArg: 4.663 ± 0.037	8.615SerSer: 8.615 ± 0.063	5.71SerThr: 5.71 ± 0.039	4.728SerVal: 4.728 ± 0.032	1.087SerTrp: 1.087 ± 0.016	2.051SerTyr: 2.051 ± 0.018	0.0SerXaa: 0.0 ± 0.0
Thr	4.746ThrAla: 4.746 ± 0.027	0.849ThrCys: 0.849 ± 0.013	2.839ThrAsp: 2.839 ± 0.022	2.986ThrGlu: 2.986 ± 0.023	2.507ThrPhe: 2.507 ± 0.02	3.812ThrGly: 3.812 ± 0.027	1.678ThrHis: 1.678 ± 0.019	3.622ThrIle: 3.622 ± 0.029	2.629ThrLys: 2.629 ± 0.02	5.866ThrLeu: 5.866 ± 0.03	1.245ThrMet: 1.245 ± 0.012	2.256ThrAsn: 2.256 ± 0.02	4.428ThrPro: 4.428 ± 0.032	2.225ThrGln: 2.225 ± 0.021	3.201ThrArg: 3.201 ± 0.026	5.532ThrSer: 5.532 ± 0.033	4.252ThrThr: 4.252 ± 0.036	3.891ThrVal: 3.891 ± 0.022	0.865ThrTrp: 0.865 ± 0.011	1.629ThrTyr: 1.629 ± 0.015	0.0ThrXaa: 0.0 ± 0.0
Val	4.594ValAla: 4.594 ± 0.027	0.904ValCys: 0.904 ± 0.011	3.476ValAsp: 3.476 ± 0.025	3.653ValGlu: 3.653 ± 0.031	2.438ValPhe: 2.438 ± 0.019	3.39ValGly: 3.39 ± 0.021	1.55ValHis: 1.55 ± 0.013	3.416ValIle: 3.416 ± 0.025	2.984ValLys: 2.984 ± 0.025	5.561ValLeu: 5.561 ± 0.028	1.26ValMet: 1.26 ± 0.012	2.451ValAsn: 2.451 ± 0.022	3.649ValPro: 3.649 ± 0.022	2.447ValGln: 2.447 ± 0.018	3.058ValArg: 3.058 ± 0.023	4.555ValSer: 4.555 ± 0.028	3.523ValThr: 3.523 ± 0.026	3.878ValVal: 3.878 ± 0.026	0.966ValTrp: 0.966 ± 0.013	1.703ValTyr: 1.703 ± 0.014	0.0ValXaa: 0.0 ± 0.0
Trp	1.067TrpAla: 1.067 ± 0.014	0.242TrpCys: 0.242 ± 0.005	0.958TrpAsp: 0.958 ± 0.013	0.898TrpGlu: 0.898 ± 0.012	0.64TrpPhe: 0.64 ± 0.009	0.834TrpGly: 0.834 ± 0.011	0.399TrpHis: 0.399 ± 0.007	0.928TrpIle: 0.928 ± 0.012	0.972TrpLys: 0.972 ± 0.012	1.439TrpLeu: 1.439 ± 0.016	0.456TrpMet: 0.456 ± 0.01	0.768TrpAsn: 0.768 ± 0.013	0.674TrpPro: 0.674 ± 0.009	0.558TrpGln: 0.558 ± 0.009	0.934TrpArg: 0.934 ± 0.013	1.263TrpSer: 1.263 ± 0.016	0.944TrpThr: 0.944 ± 0.011	0.911TrpVal: 0.911 ± 0.012	0.283TrpTrp: 0.283 ± 0.007	0.432TrpTyr: 0.432 ± 0.007	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.009TyrAla: 2.009 ± 0.018	0.462TyrCys: 0.462 ± 0.008	1.634TyrAsp: 1.634 ± 0.016	1.497TyrGlu: 1.497 ± 0.016	1.258TyrPhe: 1.258 ± 0.014	1.878TyrGly: 1.878 ± 0.015	0.844TyrHis: 0.844 ± 0.011	1.684TyrIle: 1.684 ± 0.017	1.155TyrLys: 1.155 ± 0.011	2.767TyrLeu: 2.767 ± 0.022	0.713TyrMet: 0.713 ± 0.011	1.172TyrAsn: 1.172 ± 0.012	1.602TyrPro: 1.602 ± 0.019	1.096TyrGln: 1.096 ± 0.015	1.534TyrArg: 1.534 ± 0.015	2.144TyrSer: 2.144 ± 0.018	1.619TyrThr: 1.619 ± 0.016	1.569TyrVal: 1.569 ± 0.016	0.518TyrTrp: 0.518 ± 0.008	0.982TyrTyr: 0.982 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 24609 proteins (7189526 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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