Phaeobacter sp. 22II1-1F12B
Average proteome isoelectric point is 5.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4790 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A254QXN8|A0A254QXN8_9RHOB Cytochrome P450 OS=Phaeobacter sp. 22II1-1F12B OX=1317111 GN=ATO1_00875 PE=3 SV=1
MM1 pKa = 7.49 NYY3 pKa = 9.72 VLEE6 pKa = 4.37 TDD8 pKa = 3.41 NVGVNIQDD16 pKa = 3.28 IALSGDD22 pKa = 3.22 FTIEE26 pKa = 3.64 FNVYY30 pKa = 9.86 FEE32 pKa = 5.12 PGSEE36 pKa = 3.67 IAKK39 pKa = 10.15 NDD41 pKa = 4.38 GIVSSNVDD49 pKa = 3.21 GGNDD53 pKa = 3.02 INFFGGIPRR62 pKa = 11.84 LYY64 pKa = 9.89 DD65 pKa = 3.79 ANWGDD70 pKa = 3.92 VVVGTSALNTGEE82 pKa = 4.03 WNHH85 pKa = 5.91 IAFVRR90 pKa = 11.84 EE91 pKa = 3.99 GGVVRR96 pKa = 11.84 LYY98 pKa = 11.61 VNGVNDD104 pKa = 4.68 ANSTTANYY112 pKa = 9.83 TDD114 pKa = 3.61 VFNISTLATSVAGALEE130 pKa = 4.06 GRR132 pKa = 11.84 LDD134 pKa = 3.76 EE135 pKa = 4.26 VRR137 pKa = 11.84 IWSLARR143 pKa = 11.84 PGAEE147 pKa = 3.53 VLANADD153 pKa = 3.34 GALNLSGGVPVGLEE167 pKa = 3.43 RR168 pKa = 11.84 YY169 pKa = 9.52 YY170 pKa = 11.13 RR171 pKa = 11.84 FDD173 pKa = 4.68 DD174 pKa = 5.09 DD175 pKa = 3.82 IGHH178 pKa = 6.68 IVDD181 pKa = 3.4 ATGNAEE187 pKa = 4.24 TGHH190 pKa = 6.91 DD191 pKa = 3.87 LHH193 pKa = 7.08 PPTGTSIIQDD203 pKa = 3.36 DD204 pKa = 4.76 TVFDD208 pKa = 4.12 TPVGDD213 pKa = 3.25 VGGFIDD219 pKa = 3.89 TKK221 pKa = 10.87 VVTGLVLPTDD231 pKa = 4.15 LAFLPDD237 pKa = 3.34 GRR239 pKa = 11.84 ALVIQKK245 pKa = 10.21 SGEE248 pKa = 3.97 VLLIEE253 pKa = 5.17 DD254 pKa = 4.36 PSVANSTKK262 pKa = 9.69 TLYY265 pKa = 9.69 MDD267 pKa = 5.5 LSAQVEE273 pKa = 4.22 NSRR276 pKa = 11.84 EE277 pKa = 3.98 AGLLNVVVDD286 pKa = 5.01 PNFDD290 pKa = 4.07 DD291 pKa = 4.07 NGYY294 pKa = 10.69 FYY296 pKa = 11.57 LLYY299 pKa = 10.71 TNLDD303 pKa = 3.61 EE304 pKa = 5.18 QRR306 pKa = 11.84 TTVSRR311 pKa = 11.84 FQHH314 pKa = 5.36 QEE316 pKa = 3.41 NAGGSTSTGDD326 pKa = 3.15 ISSEE330 pKa = 4.12 TVLWRR335 pKa = 11.84 EE336 pKa = 3.34 FDD338 pKa = 3.54 TYY340 pKa = 10.84 SEE342 pKa = 4.71 KK343 pKa = 10.95 DD344 pKa = 3.32 HH345 pKa = 6.66 QGGGLAVAYY354 pKa = 9.52 EE355 pKa = 4.82 PIDD358 pKa = 4.13 ANDD361 pKa = 3.74 PSPYY365 pKa = 10.31 KK366 pKa = 10.76 LYY368 pKa = 9.27 ITVGEE373 pKa = 4.15 EE374 pKa = 4.29 FEE376 pKa = 4.66 GEE378 pKa = 3.76 NAADD382 pKa = 3.92 LTHH385 pKa = 7.77 DD386 pKa = 4.09 DD387 pKa = 4.32 GKK389 pKa = 10.21 VHH391 pKa = 7.77 RR392 pKa = 11.84 INLTDD397 pKa = 3.96 GSIPTDD403 pKa = 3.26 NPYY406 pKa = 10.86 YY407 pKa = 10.56 DD408 pKa = 3.82 PAVAATYY415 pKa = 8.06 TPWINTQTAISTSAANTAIDD435 pKa = 3.93 PEE437 pKa = 4.81 GVLTTVYY444 pKa = 10.58 SYY446 pKa = 11.56 GLRR449 pKa = 11.84 NPFRR453 pKa = 11.84 AEE455 pKa = 3.65 YY456 pKa = 9.84 DD457 pKa = 3.72 QVSGTLWIGEE467 pKa = 4.03 VGGNGRR473 pKa = 11.84 YY474 pKa = 9.62 ASEE477 pKa = 4.76 DD478 pKa = 3.14 VHH480 pKa = 7.98 IAYY483 pKa = 9.26 PGANMGWPSEE493 pKa = 4.12 EE494 pKa = 5.24 GYY496 pKa = 11.08 LSDD499 pKa = 5.53 PNDD502 pKa = 4.24 PGNPIHH508 pKa = 7.14 SYY510 pKa = 9.54 IHH512 pKa = 6.8 ASAPGQDD519 pKa = 3.43 QLPSYY524 pKa = 9.73 GDD526 pKa = 3.51 SGSSISGGVVYY537 pKa = 10.66 RR538 pKa = 11.84 GTDD541 pKa = 3.62 FPEE544 pKa = 4.42 EE545 pKa = 3.68 FQGAYY550 pKa = 10.14 FYY552 pKa = 11.49 GDD554 pKa = 3.16 WVRR557 pKa = 11.84 NWIRR561 pKa = 11.84 YY562 pKa = 9.4 LEE564 pKa = 4.05 VDD566 pKa = 3.77 YY567 pKa = 11.6 SSGEE571 pKa = 3.99 PVVVSDD577 pKa = 4.04 NHH579 pKa = 6.25 FKK581 pKa = 10.96 NATGQVLTFAEE592 pKa = 4.71 APDD595 pKa = 3.5 GTLYY599 pKa = 11.18 YY600 pKa = 9.49 ITTFQTGNIFNFEE613 pKa = 3.95 GAVNRR618 pKa = 11.84 LEE620 pKa = 4.01 YY621 pKa = 10.8 SSTNTAPAGTGIILDD636 pKa = 4.16 PGEE639 pKa = 4.41 EE640 pKa = 4.05 ASLTAPHH647 pKa = 6.45 TVTFEE652 pKa = 3.91 TDD654 pKa = 2.92 AFDD657 pKa = 5.13 PDD659 pKa = 3.82 GDD661 pKa = 4.68 AITYY665 pKa = 7.36 EE666 pKa = 4.18 WSFGDD671 pKa = 4.97 GPDD674 pKa = 3.74 IDD676 pKa = 4.88 GDD678 pKa = 4.48 GIGDD682 pKa = 4.03 GATSTEE688 pKa = 4.08 MNPTYY693 pKa = 10.21 TYY695 pKa = 10.21 TSEE698 pKa = 3.99 GQYY701 pKa = 9.53 TVEE704 pKa = 5.79 LIVTDD709 pKa = 4.01 ANGAQTVYY717 pKa = 9.7 NPKK720 pKa = 10.37 VITVGEE726 pKa = 4.19 APVVTINTPTQGQTFRR742 pKa = 11.84 AGEE745 pKa = 4.17 TVIFTGSAFDD755 pKa = 4.47 ANDD758 pKa = 3.66 GVLTGEE764 pKa = 4.14 DD765 pKa = 3.85 VFWSVNLAHH774 pKa = 6.7 NEE776 pKa = 4.05 HH777 pKa = 6.31 YY778 pKa = 10.4 HH779 pKa = 6.21 PEE781 pKa = 3.92 VSAVEE786 pKa = 4.02 NTPGGFSFVVPTDD799 pKa = 2.97 GHH801 pKa = 6.92 DD802 pKa = 3.32 YY803 pKa = 10.49 YY804 pKa = 11.26 EE805 pKa = 4.29 NVGYY809 pKa = 9.91 QVSLTAIDD817 pKa = 3.75 SSGLRR822 pKa = 11.84 TTQSVTIFPEE832 pKa = 4.33 EE833 pKa = 4.41 SITTYY838 pKa = 11.48 DD839 pKa = 3.57 MPDD842 pKa = 2.76 VSGFNFFIDD851 pKa = 4.97 GIGYY855 pKa = 10.11 SGDD858 pKa = 3.36 QVRR861 pKa = 11.84 DD862 pKa = 3.58 NIIGFEE868 pKa = 4.18 HH869 pKa = 6.05 TVSVQASYY877 pKa = 11.21 IDD879 pKa = 5.35 AGFLWEE885 pKa = 4.43 FSHH888 pKa = 7.29 WEE890 pKa = 4.22 DD891 pKa = 5.15 DD892 pKa = 3.84 PSLTSTSRR900 pKa = 11.84 QFIVPEE906 pKa = 4.0 TDD908 pKa = 3.04 SVLRR912 pKa = 11.84 PVYY915 pKa = 10.52 AQTDD919 pKa = 3.67 VVGPVNAEE927 pKa = 3.7 DD928 pKa = 4.63 DD929 pKa = 3.92 VFARR933 pKa = 11.84 DD934 pKa = 3.55 LASLNGNQIQTIGNVLLNDD953 pKa = 4.73 DD954 pKa = 5.05 NPTGDD959 pKa = 3.57 PFSVVGLNGYY969 pKa = 9.46 KK970 pKa = 8.97 VTGAPKK976 pKa = 10.01 TDD978 pKa = 3.51 DD979 pKa = 4.49 TIDD982 pKa = 3.53 SNNKK986 pKa = 9.37 YY987 pKa = 9.77 GWIWGDD993 pKa = 2.82 QGGQFRR999 pKa = 11.84 VNANGTVEE1007 pKa = 4.3 FRR1009 pKa = 11.84 DD1010 pKa = 3.65 RR1011 pKa = 11.84 DD1012 pKa = 3.69 LQFAGLTAGQSITTTINYY1030 pKa = 9.57 LIADD1034 pKa = 4.03 GDD1036 pKa = 4.11 DD1037 pKa = 3.38 TDD1039 pKa = 4.07 EE1040 pKa = 5.08 ASLSLTINGAGTANTAPTGAGILLDD1065 pKa = 4.27 PGEE1068 pKa = 5.16 DD1069 pKa = 3.46 SSASAPHH1076 pKa = 6.16 TVTFDD1081 pKa = 3.38 SDD1083 pKa = 3.55 VTDD1086 pKa = 4.31 AEE1088 pKa = 4.51 GHH1090 pKa = 4.92 TLSYY1094 pKa = 10.9 LWDD1097 pKa = 4.63 FGDD1100 pKa = 4.09 GTTSTLAAPEE1110 pKa = 4.05 HH1111 pKa = 6.35 TYY1113 pKa = 11.2 SVVGFYY1119 pKa = 9.63 TVSLTVTDD1127 pKa = 3.8 EE1128 pKa = 4.45 LGAEE1132 pKa = 4.29 TVFDD1136 pKa = 4.14 EE1137 pKa = 4.98 VSIDD1141 pKa = 3.59 VGNAPPVAVADD1152 pKa = 4.07 SFTLPVGFDD1161 pKa = 3.25 PMANQLNILDD1171 pKa = 4.44 NDD1173 pKa = 3.84 YY1174 pKa = 11.65 DD1175 pKa = 4.41 PDD1177 pKa = 4.46 GSLNMQIVEE1186 pKa = 4.31 IVSGPSFGTVEE1197 pKa = 4.2 ILDD1200 pKa = 4.17 AAQEE1204 pKa = 4.02 LSDD1207 pKa = 3.95 RR1208 pKa = 11.84 GLPAGQYY1215 pKa = 9.6 GQAEE1219 pKa = 4.43 YY1220 pKa = 9.22 TAFDD1224 pKa = 4.6 PNFEE1228 pKa = 4.29 GTDD1231 pKa = 3.23 SFTYY1235 pKa = 10.08 RR1236 pKa = 11.84 VQDD1239 pKa = 4.09 NEE1241 pKa = 4.42 GMWSNIVTAEE1251 pKa = 3.85 IVVQNAPVLTLDD1263 pKa = 4.81 AVDD1266 pKa = 4.66 DD1267 pKa = 5.07 GYY1269 pKa = 11.97 AVLVADD1275 pKa = 4.48 IASGSWVQIQGNIFDD1290 pKa = 5.09 NDD1292 pKa = 3.72 TSDD1295 pKa = 3.45 GALNLLQLDD1304 pKa = 3.92 GFKK1307 pKa = 10.9 PFDD1310 pKa = 3.86 VPDD1313 pKa = 3.63 STTSFDD1319 pKa = 3.37 SQGRR1323 pKa = 11.84 LKK1325 pKa = 10.26 IRR1327 pKa = 11.84 ADD1329 pKa = 2.97 NGGFFRR1335 pKa = 11.84 IGSDD1339 pKa = 3.27 GSIEE1343 pKa = 4.15 FKK1345 pKa = 11.03 DD1346 pKa = 3.45 RR1347 pKa = 11.84 DD1348 pKa = 3.5 FDD1350 pKa = 4.03 FTGLSSSEE1358 pKa = 4.27 SAVTTISYY1366 pKa = 8.85 TIGDD1370 pKa = 4.0 GTDD1373 pKa = 2.78 TDD1375 pKa = 3.91 AAFISLTINGSGNVNTAPTGAGILLDD1401 pKa = 4.45 PGEE1404 pKa = 5.24 DD1405 pKa = 3.5 SATSAPHH1412 pKa = 5.85 TVTFDD1417 pKa = 3.38 SDD1419 pKa = 3.55 VTDD1422 pKa = 4.31 AEE1424 pKa = 4.51 GHH1426 pKa = 4.92 TLSYY1430 pKa = 10.9 LWDD1433 pKa = 4.63 FGDD1436 pKa = 4.09 GTTSTLAAPEE1446 pKa = 4.01 HH1447 pKa = 6.5 TYY1449 pKa = 11.53 NSIGFYY1455 pKa = 10.33 NVSLTVTDD1463 pKa = 3.91 EE1464 pKa = 4.29 LGAATVFNEE1473 pKa = 3.69 VSIDD1477 pKa = 3.6 VGNAPPVAVADD1488 pKa = 4.07 SFTLPVGFDD1497 pKa = 3.25 PVANHH1502 pKa = 6.61 LHH1504 pKa = 6.47 ILDD1507 pKa = 4.24 NDD1509 pKa = 3.77 YY1510 pKa = 11.66 DD1511 pKa = 4.51 PDD1513 pKa = 4.43 GSLNMQVVEE1522 pKa = 4.4 IVSGPSFGTVEE1533 pKa = 4.15 MMDD1536 pKa = 3.89 TAQEE1540 pKa = 3.95 LADD1543 pKa = 4.09 RR1544 pKa = 11.84 GLPAGHH1550 pKa = 6.59 YY1551 pKa = 9.77 GHH1553 pKa = 7.49 AEE1555 pKa = 3.93 YY1556 pKa = 8.51 ATFDD1560 pKa = 4.19 PNFEE1564 pKa = 4.26 GTDD1567 pKa = 3.23 SFTYY1571 pKa = 10.08 RR1572 pKa = 11.84 VQDD1575 pKa = 4.9 NEE1577 pKa = 4.61 GTWSNIVTAEE1587 pKa = 4.14 INVQSAPSTPGGALASVNSGSGLDD1611 pKa = 3.42 IQDD1614 pKa = 3.13 ISVAGDD1620 pKa = 3.16 FTVEE1624 pKa = 3.61 FWIAFNPGTNTNKK1637 pKa = 9.98 YY1638 pKa = 10.18 DD1639 pKa = 3.35 SAFAGGVYY1647 pKa = 10.36 DD1648 pKa = 5.06 DD1649 pKa = 5.07 TPGDD1653 pKa = 3.94 DD1654 pKa = 3.91 PSTDD1658 pKa = 3.15 GVFGNDD1664 pKa = 3.24 FNFHH1668 pKa = 6.56 AGLARR1673 pKa = 11.84 LYY1675 pKa = 9.66 STEE1678 pKa = 4.03 IPSGGDD1684 pKa = 3.08 AIVANTALTSNGVANHH1700 pKa = 5.81 VALVRR1705 pKa = 11.84 EE1706 pKa = 4.52 GNNFSIYY1713 pKa = 9.8 MNGVLDD1719 pKa = 4.0 STSTLALDD1727 pKa = 3.78 GDD1729 pKa = 4.35 LLIEE1733 pKa = 4.15 RR1734 pKa = 11.84 LATNVRR1740 pKa = 11.84 ATKK1743 pKa = 10.38 ALSGTLDD1750 pKa = 3.61 EE1751 pKa = 5.02 FRR1753 pKa = 11.84 VWDD1756 pKa = 4.08 DD1757 pKa = 3.25 ARR1759 pKa = 11.84 SASEE1763 pKa = 3.29 IATYY1767 pKa = 9.84 MNQEE1771 pKa = 4.12 VASNADD1777 pKa = 3.34 DD1778 pKa = 4.02 LLRR1781 pKa = 11.84 YY1782 pKa = 9.17 YY1783 pKa = 10.78 QFDD1786 pKa = 4.42 DD1787 pKa = 3.59 DD1788 pKa = 4.58 TFVIDD1793 pKa = 3.44 STGNAEE1799 pKa = 4.05 AAGDD1803 pKa = 3.85 LSYY1806 pKa = 11.72 ASISGVDD1813 pKa = 3.03 WFEE1816 pKa = 4.01 FSDD1819 pKa = 3.46 WLGAA1823 pKa = 3.78
Molecular weight: 194.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.668
IPC_protein 3.732
Toseland 3.49
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.541
Grimsley 3.401
Solomon 3.719
Lehninger 3.681
Nozaki 3.821
DTASelect 4.101
Thurlkill 3.541
EMBOSS 3.668
Sillero 3.846
Patrickios 0.922
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.749
Protein with the highest isoelectric point:
>tr|A0A254QLH7|A0A254QLH7_9RHOB Sorbitol dehydrogenase OS=Phaeobacter sp. 22II1-1F12B OX=1317111 GN=ATO1_20225 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.43 EE41 pKa = 3.72 LSAA44 pKa = 5.03
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.423
IPC2_protein 10.906
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.13
Rodwell 12.34
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.076
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.092
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4790
0
4790
1505669
31
2868
314.3
34.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.513 ± 0.05
0.899 ± 0.012
6.084 ± 0.034
6.294 ± 0.035
3.795 ± 0.023
8.573 ± 0.036
2.012 ± 0.02
5.365 ± 0.026
3.342 ± 0.026
9.968 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.887 ± 0.017
2.747 ± 0.017
5.012 ± 0.024
3.181 ± 0.018
6.551 ± 0.036
5.529 ± 0.022
5.378 ± 0.02
7.166 ± 0.028
1.392 ± 0.015
2.312 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here