Amino acid dipepetide frequency for Phaeobacter sp. 22II1-1F12B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.368AlaAla: 14.368 ± 0.144
1.084AlaCys: 1.084 ± 0.026
6.469AlaAsp: 6.469 ± 0.077
8.125AlaGlu: 8.125 ± 0.102
4.176AlaPhe: 4.176 ± 0.053
9.622AlaGly: 9.622 ± 0.088
2.134AlaHis: 2.134 ± 0.04
5.844AlaIle: 5.844 ± 0.069
3.814AlaLys: 3.814 ± 0.062
12.794AlaLeu: 12.794 ± 0.113
3.698AlaMet: 3.698 ± 0.053
2.76AlaAsn: 2.76 ± 0.045
5.273AlaPro: 5.273 ± 0.068
4.106AlaGln: 4.106 ± 0.055
8.02AlaArg: 8.02 ± 0.09
5.681AlaSer: 5.681 ± 0.07
5.552AlaThr: 5.552 ± 0.063
7.735AlaVal: 7.735 ± 0.077
1.338AlaTrp: 1.338 ± 0.027
2.534AlaTyr: 2.534 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
1.049CysAla: 1.049 ± 0.027
0.128CysCys: 0.128 ± 0.011
0.629CysAsp: 0.629 ± 0.022
0.485CysGlu: 0.485 ± 0.019
0.345CysPhe: 0.345 ± 0.016
0.935CysGly: 0.935 ± 0.027
0.273CysHis: 0.273 ± 0.014
0.432CysIle: 0.432 ± 0.014
0.25CysLys: 0.25 ± 0.012
0.871CysLeu: 0.871 ± 0.019
0.172CysMet: 0.172 ± 0.011
0.23CysAsn: 0.23 ± 0.011
0.511CysPro: 0.511 ± 0.02
0.215CysGln: 0.215 ± 0.012
0.536CysArg: 0.536 ± 0.021
0.459CysSer: 0.459 ± 0.021
0.46CysThr: 0.46 ± 0.02
0.661CysVal: 0.661 ± 0.022
0.123CysTrp: 0.123 ± 0.009
0.223CysTyr: 0.223 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
6.731AspAla: 6.731 ± 0.07
0.541AspCys: 0.541 ± 0.02
3.453AspAsp: 3.453 ± 0.078
3.795AspGlu: 3.795 ± 0.056
2.421AspPhe: 2.421 ± 0.04
5.315AspGly: 5.315 ± 0.08
1.398AspHis: 1.398 ± 0.028
3.298AspIle: 3.298 ± 0.049
1.901AspLys: 1.901 ± 0.043
6.502AspLeu: 6.502 ± 0.068
1.868AspMet: 1.868 ± 0.039
1.399AspAsn: 1.399 ± 0.032
3.634AspPro: 3.634 ± 0.055
1.96AspGln: 1.96 ± 0.033
4.239AspArg: 4.239 ± 0.066
2.455AspSer: 2.455 ± 0.048
3.019AspThr: 3.019 ± 0.053
4.257AspVal: 4.257 ± 0.057
1.144AspTrp: 1.144 ± 0.025
1.507AspTyr: 1.507 ± 0.032
0.0AspXaa: 0.0 ± 0.0
Glu
7.589GluAla: 7.589 ± 0.091
0.402GluCys: 0.402 ± 0.017
3.653GluAsp: 3.653 ± 0.046
4.176GluGlu: 4.176 ± 0.065
2.081GluPhe: 2.081 ± 0.041
4.692GluGly: 4.692 ± 0.069
1.201GluHis: 1.201 ± 0.029
4.072GluIle: 4.072 ± 0.055
2.645GluLys: 2.645 ± 0.048
5.617GluLeu: 5.617 ± 0.065
2.057GluMet: 2.057 ± 0.04
2.142GluAsn: 2.142 ± 0.042
2.739GluPro: 2.739 ± 0.048
2.188GluGln: 2.188 ± 0.038
4.386GluArg: 4.386 ± 0.061
2.732GluSer: 2.732 ± 0.041
4.243GluThr: 4.243 ± 0.056
4.33GluVal: 4.33 ± 0.053
0.795GluTrp: 0.795 ± 0.026
1.197GluTyr: 1.197 ± 0.024
0.0GluXaa: 0.0 ± 0.0
Phe
4.387PheAla: 4.387 ± 0.063
0.467PheCys: 0.467 ± 0.016
2.944PheAsp: 2.944 ± 0.048
2.347PheGlu: 2.347 ± 0.038
1.499PhePhe: 1.499 ± 0.039
3.749PheGly: 3.749 ± 0.047
0.731PheHis: 0.731 ± 0.023
1.708PheIle: 1.708 ± 0.038
0.996PheLys: 0.996 ± 0.025
3.549PheLeu: 3.549 ± 0.06
0.903PheMet: 0.903 ± 0.023
1.063PheAsn: 1.063 ± 0.024
1.591PhePro: 1.591 ± 0.031
1.089PheGln: 1.089 ± 0.026
2.23PheArg: 2.23 ± 0.041
2.34PheSer: 2.34 ± 0.041
2.07PheThr: 2.07 ± 0.039
2.728PheVal: 2.728 ± 0.046
0.591PheTrp: 0.591 ± 0.019
0.97PheTyr: 0.97 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
9.066GlyAla: 9.066 ± 0.079
0.861GlyCys: 0.861 ± 0.023
4.737GlyAsp: 4.737 ± 0.066
5.085GlyGlu: 5.085 ± 0.062
3.713GlyPhe: 3.713 ± 0.059
7.28GlyGly: 7.28 ± 0.093
1.908GlyHis: 1.908 ± 0.038
4.607GlyIle: 4.607 ± 0.065
3.33GlyLys: 3.33 ± 0.057
8.993GlyLeu: 8.993 ± 0.097
2.732GlyMet: 2.732 ± 0.042
2.27GlyAsn: 2.27 ± 0.057
3.552GlyPro: 3.552 ± 0.05
3.091GlyGln: 3.091 ± 0.05
5.436GlyArg: 5.436 ± 0.063
4.498GlySer: 4.498 ± 0.064
4.538GlyThr: 4.538 ± 0.056
6.142GlyVal: 6.142 ± 0.061
1.464GlyTrp: 1.464 ± 0.036
2.42GlyTyr: 2.42 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
2.159HisAla: 2.159 ± 0.041
0.24HisCys: 0.24 ± 0.014
1.269HisAsp: 1.269 ± 0.03
1.121HisGlu: 1.121 ± 0.028
0.861HisPhe: 0.861 ± 0.022
1.811HisGly: 1.811 ± 0.032
0.535HisHis: 0.535 ± 0.021
0.984HisIle: 0.984 ± 0.024
0.535HisLys: 0.535 ± 0.019
2.044HisLeu: 2.044 ± 0.038
0.566HisMet: 0.566 ± 0.019
0.468HisAsn: 0.468 ± 0.019
1.334HisPro: 1.334 ± 0.033
0.574HisGln: 0.574 ± 0.019
1.352HisArg: 1.352 ± 0.03
0.972HisSer: 0.972 ± 0.027
0.841HisThr: 0.841 ± 0.026
1.549HisVal: 1.549 ± 0.033
0.356HisTrp: 0.356 ± 0.015
0.548HisTyr: 0.548 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
6.98IleAla: 6.98 ± 0.085
0.639IleCys: 0.639 ± 0.021
3.655IleAsp: 3.655 ± 0.048
3.731IleGlu: 3.731 ± 0.053
1.832IlePhe: 1.832 ± 0.039
5.08IleGly: 5.08 ± 0.063
0.954IleHis: 0.954 ± 0.026
2.397IleIle: 2.397 ± 0.052
1.495IleLys: 1.495 ± 0.03
4.919IleLeu: 4.919 ± 0.071
1.177IleMet: 1.177 ± 0.029
1.487IleAsn: 1.487 ± 0.034
2.389IlePro: 2.389 ± 0.039
1.292IleGln: 1.292 ± 0.029
3.295IleArg: 3.295 ± 0.046
3.377IleSer: 3.377 ± 0.045
2.987IleThr: 2.987 ± 0.045
3.91IleVal: 3.91 ± 0.06
0.787IleTrp: 0.787 ± 0.025
1.266IleTyr: 1.266 ± 0.03
0.0IleXaa: 0.0 ± 0.0
Lys
3.934LysAla: 3.934 ± 0.065
0.193LysCys: 0.193 ± 0.011
1.799LysAsp: 1.799 ± 0.041
1.781LysGlu: 1.781 ± 0.042
0.999LysPhe: 0.999 ± 0.027
2.899LysGly: 2.899 ± 0.049
0.632LysHis: 0.632 ± 0.022
1.813LysIle: 1.813 ± 0.032
1.336LysLys: 1.336 ± 0.037
3.283LysLeu: 3.283 ± 0.048
0.954LysMet: 0.954 ± 0.025
0.926LysAsn: 0.926 ± 0.027
1.872LysPro: 1.872 ± 0.042
1.006LysGln: 1.006 ± 0.027
2.298LysArg: 2.298 ± 0.041
2.087LysSer: 2.087 ± 0.038
2.083LysThr: 2.083 ± 0.037
2.401LysVal: 2.401 ± 0.042
0.427LysTrp: 0.427 ± 0.018
0.701LysTyr: 0.701 ± 0.025
0.0LysXaa: 0.0 ± 0.0
Leu
12.311LeuAla: 12.311 ± 0.105
0.951LeuCys: 0.951 ± 0.027
5.978LeuAsp: 5.978 ± 0.072
5.578LeuGlu: 5.578 ± 0.072
3.574LeuPhe: 3.574 ± 0.066
8.366LeuGly: 8.366 ± 0.076
1.837LeuHis: 1.837 ± 0.036
5.488LeuIle: 5.488 ± 0.072
3.369LeuLys: 3.369 ± 0.059
8.823LeuLeu: 8.823 ± 0.116
2.691LeuMet: 2.691 ± 0.044
2.956LeuAsn: 2.956 ± 0.049
5.438LeuPro: 5.438 ± 0.075
2.705LeuGln: 2.705 ± 0.042
6.739LeuArg: 6.739 ± 0.077
6.879LeuSer: 6.879 ± 0.077
5.774LeuThr: 5.774 ± 0.055
6.916LeuVal: 6.916 ± 0.075
1.268LeuTrp: 1.268 ± 0.031
2.042LeuTyr: 2.042 ± 0.043
0.0LeuXaa: 0.0 ± 0.0
Met
3.433MetAla: 3.433 ± 0.045
0.179MetCys: 0.179 ± 0.011
1.449MetAsp: 1.449 ± 0.034
1.483MetGlu: 1.483 ± 0.032
0.887MetPhe: 0.887 ± 0.025
2.307MetGly: 2.307 ± 0.043
0.491MetHis: 0.491 ± 0.018
1.689MetIle: 1.689 ± 0.035
1.206MetLys: 1.206 ± 0.029
2.798MetLeu: 2.798 ± 0.047
0.84MetMet: 0.84 ± 0.025
0.961MetAsn: 0.961 ± 0.027
1.583MetPro: 1.583 ± 0.032
1.062MetGln: 1.062 ± 0.023
1.973MetArg: 1.973 ± 0.037
1.965MetSer: 1.965 ± 0.035
2.052MetThr: 2.052 ± 0.036
1.878MetVal: 1.878 ± 0.034
0.24MetTrp: 0.24 ± 0.015
0.398MetTyr: 0.398 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.211AsnAla: 3.211 ± 0.049
0.29AsnCys: 0.29 ± 0.015
1.552AsnAsp: 1.552 ± 0.042
1.375AsnGlu: 1.375 ± 0.035
1.012AsnPhe: 1.012 ± 0.026
2.6AsnGly: 2.6 ± 0.047
0.531AsnHis: 0.531 ± 0.018
1.477AsnIle: 1.477 ± 0.03
0.773AsnLys: 0.773 ± 0.024
2.642AsnLeu: 2.642 ± 0.044
0.784AsnMet: 0.784 ± 0.021
0.736AsnAsn: 0.736 ± 0.022
1.905AsnPro: 1.905 ± 0.036
0.768AsnGln: 0.768 ± 0.025
1.913AsnArg: 1.913 ± 0.035
1.346AsnSer: 1.346 ± 0.029
1.433AsnThr: 1.433 ± 0.032
1.911AsnVal: 1.911 ± 0.038
0.503AsnTrp: 0.503 ± 0.019
0.712AsnTyr: 0.712 ± 0.022
0.0AsnXaa: 0.0 ± 0.0
Pro
5.179ProAla: 5.179 ± 0.066
0.343ProCys: 0.343 ± 0.015
3.957ProAsp: 3.957 ± 0.054
4.45ProGlu: 4.45 ± 0.063
1.916ProPhe: 1.916 ± 0.036
4.538ProGly: 4.538 ± 0.059
1.019ProHis: 1.019 ± 0.022
2.362ProIle: 2.362 ± 0.04
1.697ProLys: 1.697 ± 0.033
4.562ProLeu: 4.562 ± 0.057
1.358ProMet: 1.358 ± 0.035
1.356ProAsn: 1.356 ± 0.03
2.157ProPro: 2.157 ± 0.044
1.584ProGln: 1.584 ± 0.036
2.583ProArg: 2.583 ± 0.04
2.647ProSer: 2.647 ± 0.043
2.311ProThr: 2.311 ± 0.038
4.269ProVal: 4.269 ± 0.053
0.64ProTrp: 0.64 ± 0.02
1.193ProTyr: 1.193 ± 0.028
0.0ProXaa: 0.0 ± 0.0
Gln
3.764GlnAla: 3.764 ± 0.058
0.208GlnCys: 0.208 ± 0.011
1.737GlnAsp: 1.737 ± 0.038
1.789GlnGlu: 1.789 ± 0.036
1.127GlnPhe: 1.127 ± 0.031
2.524GlnGly: 2.524 ± 0.042
0.59GlnHis: 0.59 ± 0.023
2.076GlnIle: 2.076 ± 0.04
1.162GlnLys: 1.162 ± 0.029
2.831GlnLeu: 2.831 ± 0.046
1.114GlnMet: 1.114 ± 0.026
0.948GlnAsn: 0.948 ± 0.028
1.62GlnPro: 1.62 ± 0.035
1.14GlnGln: 1.14 ± 0.029
2.142GlnArg: 2.142 ± 0.039
1.971GlnSer: 1.971 ± 0.04
1.74GlnThr: 1.74 ± 0.035
2.32GlnVal: 2.32 ± 0.038
0.399GlnTrp: 0.399 ± 0.017
0.613GlnTyr: 0.613 ± 0.018
0.0GlnXaa: 0.0 ± 0.0
Arg
7.297ArgAla: 7.297 ± 0.082
0.486ArgCys: 0.486 ± 0.019
4.266ArgAsp: 4.266 ± 0.054
4.215ArgGlu: 4.215 ± 0.058
2.631ArgPhe: 2.631 ± 0.042
4.424ArgGly: 4.424 ± 0.065
1.481ArgHis: 1.481 ± 0.029
3.863ArgIle: 3.863 ± 0.052
2.461ArgLys: 2.461 ± 0.04
7.026ArgLeu: 7.026 ± 0.071
1.97ArgMet: 1.97 ± 0.038
1.871ArgAsn: 1.871 ± 0.034
3.205ArgPro: 3.205 ± 0.048
2.346ArgGln: 2.346 ± 0.039
4.777ArgArg: 4.777 ± 0.065
3.41ArgSer: 3.41 ± 0.049
2.857ArgThr: 2.857 ± 0.04
4.467ArgVal: 4.467 ± 0.057
0.857ArgTrp: 0.857 ± 0.026
1.601ArgTyr: 1.601 ± 0.029
0.0ArgXaa: 0.0 ± 0.0
Ser
6.054SerAla: 6.054 ± 0.071
0.466SerCys: 0.466 ± 0.017
3.719SerAsp: 3.719 ± 0.051
3.473SerGlu: 3.473 ± 0.055
2.418SerPhe: 2.418 ± 0.043
5.687SerGly: 5.687 ± 0.073
1.118SerHis: 1.118 ± 0.025
2.748SerIle: 2.748 ± 0.045
1.686SerLys: 1.686 ± 0.033
5.386SerLeu: 5.386 ± 0.072
1.528SerMet: 1.528 ± 0.033
1.512SerAsn: 1.512 ± 0.031
2.614SerPro: 2.614 ± 0.045
1.654SerGln: 1.654 ± 0.035
3.456SerArg: 3.456 ± 0.056
2.91SerSer: 2.91 ± 0.054
2.603SerThr: 2.603 ± 0.044
4.067SerVal: 4.067 ± 0.05
0.768SerTrp: 0.768 ± 0.022
1.425SerTyr: 1.425 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
5.708ThrAla: 5.708 ± 0.074
0.477ThrCys: 0.477 ± 0.018
3.086ThrAsp: 3.086 ± 0.051
3.123ThrGlu: 3.123 ± 0.049
1.965ThrPhe: 1.965 ± 0.036
5.424ThrGly: 5.424 ± 0.067
1.054ThrHis: 1.054 ± 0.029
2.764ThrIle: 2.764 ± 0.051
1.485ThrLys: 1.485 ± 0.031
5.787ThrLeu: 5.787 ± 0.061
1.321ThrMet: 1.321 ± 0.03
1.307ThrAsn: 1.307 ± 0.031
3.332ThrPro: 3.332 ± 0.053
1.575ThrGln: 1.575 ± 0.034
3.41ThrArg: 3.41 ± 0.048
2.908ThrSer: 2.908 ± 0.051
2.764ThrThr: 2.764 ± 0.048
4.233ThrVal: 4.233 ± 0.058
0.707ThrTrp: 0.707 ± 0.023
1.347ThrTyr: 1.347 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
8.134ValAla: 8.134 ± 0.071
0.635ValCys: 0.635 ± 0.019
4.03ValAsp: 4.03 ± 0.053
4.65ValGlu: 4.65 ± 0.061
2.927ValPhe: 2.927 ± 0.051
5.241ValGly: 5.241 ± 0.061
1.332ValHis: 1.332 ± 0.034
4.328ValIle: 4.328 ± 0.057
2.17ValLys: 2.17 ± 0.046
7.306ValLeu: 7.306 ± 0.087
2.218ValMet: 2.218 ± 0.04
2.004ValAsn: 2.004 ± 0.037
3.563ValPro: 3.563 ± 0.054
2.127ValGln: 2.127 ± 0.033
4.05ValArg: 4.05 ± 0.053
4.551ValSer: 4.551 ± 0.057
4.59ValThr: 4.59 ± 0.051
5.394ValVal: 5.394 ± 0.071
0.928ValTrp: 0.928 ± 0.024
1.481ValTyr: 1.481 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.291TrpAla: 1.291 ± 0.035
0.148TrpCys: 0.148 ± 0.01
0.745TrpAsp: 0.745 ± 0.023
0.719TrpGlu: 0.719 ± 0.019
0.604TrpPhe: 0.604 ± 0.021
1.027TrpGly: 1.027 ± 0.027
0.341TrpHis: 0.341 ± 0.015
0.725TrpIle: 0.725 ± 0.025
0.491TrpLys: 0.491 ± 0.02
1.587TrpLeu: 1.587 ± 0.036
0.408TrpMet: 0.408 ± 0.017
0.448TrpAsn: 0.448 ± 0.015
0.692TrpPro: 0.692 ± 0.024
0.611TrpGln: 0.611 ± 0.022
1.062TrpArg: 1.062 ± 0.028
0.851TrpSer: 0.851 ± 0.027
0.757TrpThr: 0.757 ± 0.022
0.913TrpVal: 0.913 ± 0.025
0.201TrpTrp: 0.201 ± 0.013
0.3TrpTyr: 0.3 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.48TyrAla: 2.48 ± 0.04
0.248TyrCys: 0.248 ± 0.012
1.71TyrAsp: 1.71 ± 0.03
1.438TyrGlu: 1.438 ± 0.032
0.964TyrPhe: 0.964 ± 0.023
2.205TyrGly: 2.205 ± 0.046
0.559TyrHis: 0.559 ± 0.019
0.972TyrIle: 0.972 ± 0.027
0.621TyrLys: 0.621 ± 0.02
2.364TyrLeu: 2.364 ± 0.04
0.517TyrMet: 0.517 ± 0.019
0.631TyrAsn: 0.631 ± 0.022
1.116TyrPro: 1.116 ± 0.03
0.72TyrGln: 0.72 ± 0.021
1.616TyrArg: 1.616 ± 0.031
1.255TyrSer: 1.255 ± 0.028
1.103TyrThr: 1.103 ± 0.027
1.579TyrVal: 1.579 ± 0.033
0.383TyrTrp: 0.383 ± 0.017
0.64TyrTyr: 0.64 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4790 proteins (1505669 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski