Pseudidiomarina taiwanensis
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2021 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A432ZL29|A0A432ZL29_9GAMM Rhomboid family intramembrane serine protease OS=Pseudidiomarina taiwanensis OX=337250 GN=CWI83_06420 PE=4 SV=1
MM1 pKa = 7.37 KK2 pKa = 10.78 GIMKK6 pKa = 10.48 YY7 pKa = 10.86 GLATVAAVLLSACGSSSDD25 pKa = 4.55 DD26 pKa = 3.32 EE27 pKa = 4.77 STVVLPEE34 pKa = 5.26 LITEE38 pKa = 4.28 VRR40 pKa = 11.84 VHH42 pKa = 5.8 HH43 pKa = 6.15 TVADD47 pKa = 3.94 APNVNVLVNGGAAVEE62 pKa = 4.14 DD63 pKa = 4.0 AGFGASSTVLEE74 pKa = 4.89 LDD76 pKa = 3.13 AGTYY80 pKa = 9.93 SLQVDD85 pKa = 5.08 AILADD90 pKa = 3.92 GSTATVIGPVNDD102 pKa = 3.43 TLAANTRR109 pKa = 11.84 YY110 pKa = 10.09 EE111 pKa = 3.68 ILAIGKK117 pKa = 9.01 VADD120 pKa = 3.86 EE121 pKa = 4.79 TIQPLIITNLNSEE134 pKa = 4.22 IADD137 pKa = 3.53 GNARR141 pKa = 11.84 LQVVHH146 pKa = 7.12 AAPDD150 pKa = 3.47 APLVDD155 pKa = 4.55 IYY157 pKa = 10.72 LTAPDD162 pKa = 4.75 ADD164 pKa = 4.44 LSASSPAATLAFSDD178 pKa = 3.56 FTGQVEE184 pKa = 4.82 VPAGDD189 pKa = 3.56 YY190 pKa = 9.08 QVRR193 pKa = 11.84 ITPAGDD199 pKa = 3.2 PATVVFDD206 pKa = 4.33 SGTLPLTAGADD217 pKa = 4.09 LIVSAVTNTKK227 pKa = 10.68 AGDD230 pKa = 4.09 SPVMLQVADD239 pKa = 4.09 GSGASIVTDD248 pKa = 3.73 INAGADD254 pKa = 3.36 VRR256 pKa = 11.84 IVHH259 pKa = 6.51 SSADD263 pKa = 3.55 APTVDD268 pKa = 5.59 FVADD272 pKa = 3.3 GDD274 pKa = 4.24 MPATLATLSFGQFTDD289 pKa = 3.71 YY290 pKa = 11.54 LNVADD295 pKa = 5.45 GDD297 pKa = 4.1 YY298 pKa = 11.1 LIDD301 pKa = 4.11 VIVNGTTTVALNDD314 pKa = 3.64 IPLSLAQGEE323 pKa = 4.63 IYY325 pKa = 10.33 SVYY328 pKa = 10.69 AIGSAGDD335 pKa = 3.68 GSLTAGVLTPDD346 pKa = 3.26 TRR348 pKa = 11.84 RR349 pKa = 11.84 IATEE353 pKa = 3.67 AKK355 pKa = 9.37 LQAVHH360 pKa = 7.15 ASPTAGDD367 pKa = 3.11 VDD369 pKa = 3.89 IYY371 pKa = 10.55 VTATDD376 pKa = 5.67 DD377 pKa = 3.47 ITSATPAFTAVPFTTEE393 pKa = 4.08 NGPASTGYY401 pKa = 10.26 VSLMPGDD408 pKa = 4.28 YY409 pKa = 10.49 YY410 pKa = 10.86 VTITPTGTKK419 pKa = 9.83 DD420 pKa = 3.13 AAIGPEE426 pKa = 4.21 LVTLEE431 pKa = 4.14 GNGIYY436 pKa = 9.51 TAVAVDD442 pKa = 3.61 AVGGGVPLNVIAMDD456 pKa = 4.59 DD457 pKa = 3.9 FATVDD462 pKa = 3.14
Molecular weight: 46.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.579
IPC_protein 3.63
Toseland 3.389
ProMoST 3.795
Dawson 3.643
Bjellqvist 3.821
Wikipedia 3.605
Rodwell 3.452
Grimsley 3.3
Solomon 3.643
Lehninger 3.592
Nozaki 3.757
DTASelect 4.062
Thurlkill 3.452
EMBOSS 3.617
Sillero 3.757
Patrickios 1.252
IPC_peptide 3.63
IPC2_peptide 3.732
IPC2.peptide.svr19 3.724
Protein with the highest isoelectric point:
>tr|A0A432ZEQ9|A0A432ZEQ9_9GAMM DUF2489 domain-containing protein OS=Pseudidiomarina taiwanensis OX=337250 GN=CWI83_08455 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.49 RR12 pKa = 11.84 KK13 pKa = 7.97 RR14 pKa = 11.84 THH16 pKa = 5.89 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2021
0
2021
682925
37
1759
337.9
37.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.358 ± 0.061
0.858 ± 0.016
5.393 ± 0.046
6.354 ± 0.051
3.877 ± 0.035
6.978 ± 0.051
2.273 ± 0.027
5.665 ± 0.041
3.904 ± 0.041
10.837 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.365 ± 0.027
3.369 ± 0.032
4.292 ± 0.034
5.578 ± 0.06
5.669 ± 0.041
5.637 ± 0.036
5.121 ± 0.03
7.144 ± 0.045
1.367 ± 0.028
2.962 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here