Acinetobacter phage fLi-Aba02
Average proteome isoelectric point is 6.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6M5CB29|A0A6M5CB29_9CAUD SASA domain-containing protein OS=Acinetobacter phage fLi-Aba02 OX=2736182 GN=fLiAba02_21 PE=4 SV=1
MM1 pKa = 7.07 VKK3 pKa = 10.37 VPIFKK8 pKa = 10.47 LARR11 pKa = 11.84 ADD13 pKa = 3.76 PAVTALLEE21 pKa = 4.53 SNGILRR27 pKa = 11.84 VWKK30 pKa = 10.15 FGHH33 pKa = 7.04 APDD36 pKa = 5.7 DD37 pKa = 4.03 PQTPYY42 pKa = 9.69 VTWQTITGDD51 pKa = 3.81 SNSNLDD57 pKa = 3.71 SRR59 pKa = 11.84 PVSDD63 pKa = 4.18 NAIIQIDD70 pKa = 4.29 VYY72 pKa = 10.9 ATDD75 pKa = 3.7 EE76 pKa = 4.28 DD77 pKa = 4.38 VVEE80 pKa = 4.42 QVAIAMRR87 pKa = 11.84 DD88 pKa = 3.74 AIEE91 pKa = 4.12 LDD93 pKa = 4.18 CYY95 pKa = 8.73 VVRR98 pKa = 11.84 YY99 pKa = 10.3 GEE101 pKa = 4.11 ADD103 pKa = 3.48 KK104 pKa = 11.52 DD105 pKa = 3.93 PVTGMPHH112 pKa = 5.91 YY113 pKa = 10.74 SFDD116 pKa = 3.49 VSWIVNRR123 pKa = 11.84 EE124 pKa = 3.69
Molecular weight: 13.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.337
IPC2_protein 4.266
IPC_protein 4.215
Toseland 4.012
ProMoST 4.329
Dawson 4.202
Bjellqvist 4.418
Wikipedia 4.151
Rodwell 4.05
Grimsley 3.923
Solomon 4.202
Lehninger 4.151
Nozaki 4.317
DTASelect 4.571
Thurlkill 4.062
EMBOSS 4.151
Sillero 4.342
Patrickios 3.694
IPC_peptide 4.202
IPC2_peptide 4.317
IPC2.peptide.svr19 4.266
Protein with the highest isoelectric point:
>tr|A0A6M5CC03|A0A6M5CC03_9CAUD Uncharacterized protein OS=Acinetobacter phage fLi-Aba02 OX=2736182 GN=fLiAba02_20 PE=4 SV=1
MM1 pKa = 7.35 ATQIHH6 pKa = 6.05 GLEE9 pKa = 3.91 PALRR13 pKa = 11.84 KK14 pKa = 8.42 MQAIGNEE21 pKa = 4.14 KK22 pKa = 8.1 TVKK25 pKa = 10.3 RR26 pKa = 11.84 IARR29 pKa = 11.84 KK30 pKa = 9.94 AMRR33 pKa = 11.84 QAMNIARR40 pKa = 11.84 DD41 pKa = 3.54 EE42 pKa = 4.03 ARR44 pKa = 11.84 QKK46 pKa = 10.75 VKK48 pKa = 10.78 RR49 pKa = 11.84 LDD51 pKa = 4.27 DD52 pKa = 3.68 PTTPEE57 pKa = 4.81 KK58 pKa = 9.61 IWKK61 pKa = 9.51 EE62 pKa = 3.47 IVVQNGRR69 pKa = 11.84 SRR71 pKa = 11.84 NKK73 pKa = 7.96 NTLVMRR79 pKa = 11.84 VGVRR83 pKa = 11.84 GGARR87 pKa = 11.84 IPYY90 pKa = 8.42 TNNAQNRR97 pKa = 11.84 RR98 pKa = 11.84 SGRR101 pKa = 11.84 VGKK104 pKa = 8.62 TYY106 pKa = 9.25 QTDD109 pKa = 2.9 GRR111 pKa = 11.84 VFYY114 pKa = 10.44 WRR116 pKa = 11.84 FLEE119 pKa = 5.09 LGTSRR124 pKa = 11.84 QPATPFLRR132 pKa = 11.84 PALYY136 pKa = 10.69 EE137 pKa = 4.22 NIEE140 pKa = 4.18 QITDD144 pKa = 3.22 KK145 pKa = 10.61 FVQVFNFEE153 pKa = 4.3 LSVVLGAAA161 pKa = 3.65
Molecular weight: 18.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.307
IPC2_protein 9.882
IPC_protein 10.921
Toseland 11.096
ProMoST 11.096
Dawson 11.155
Bjellqvist 10.95
Wikipedia 11.447
Rodwell 11.272
Grimsley 11.184
Solomon 11.403
Lehninger 11.345
Nozaki 11.082
DTASelect 10.95
Thurlkill 11.096
EMBOSS 11.535
Sillero 11.096
Patrickios 10.994
IPC_peptide 11.418
IPC2_peptide 10.028
IPC2.peptide.svr19 8.669
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
10930
56
1195
202.4
22.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.271 ± 0.596
1.07 ± 0.161
6.057 ± 0.264
6.743 ± 0.266
3.641 ± 0.215
6.441 ± 0.271
1.619 ± 0.16
6.148 ± 0.305
7.466 ± 0.354
8.966 ± 0.322
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.425 ± 0.163
5.142 ± 0.326
3.532 ± 0.278
4.584 ± 0.453
4.584 ± 0.258
6.633 ± 0.423
5.947 ± 0.303
5.837 ± 0.254
1.226 ± 0.101
3.669 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here