Monodelphis domestica (Gray short-tailed opossum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Theria; Metatheria; Didelphimorphia; Didelphidae; Didelphinae; Monodelphis

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 36221 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5F8GDN3|A0A5F8GDN3_MONDO Isoform of F6TB56 Ig-like domain-containing protein OS=Monodelphis domestica OX=13616 GN=CCDC141 PE=4 SV=1
MM1 pKa = 7.51SFLKK5 pKa = 10.64SFPPPSSEE13 pKa = 4.71DD14 pKa = 3.26GLRR17 pKa = 11.84QQQPDD22 pKa = 3.8TEE24 pKa = 4.26AVLDD28 pKa = 4.25GKK30 pKa = 11.27GLGTGTLYY38 pKa = 10.36IAEE41 pKa = 4.77SRR43 pKa = 11.84LSWLDD48 pKa = 3.16GSGLGFSLDD57 pKa = 3.61YY58 pKa = 9.22PTISLHH64 pKa = 6.36AVSRR68 pKa = 11.84DD69 pKa = 3.15LNAYY73 pKa = 7.41PQEE76 pKa = 4.13HH77 pKa = 7.12LYY79 pKa = 11.62VMVNARR85 pKa = 11.84FGVEE89 pKa = 3.68ALFAAMCEE97 pKa = 4.42CQALHH102 pKa = 7.39PDD104 pKa = 4.25PEE106 pKa = 6.21DD107 pKa = 4.16EE108 pKa = 6.53DD109 pKa = 5.1SDD111 pKa = 5.45DD112 pKa = 4.29YY113 pKa = 11.91DD114 pKa = 4.07GEE116 pKa = 4.35EE117 pKa = 4.51YY118 pKa = 10.58DD119 pKa = 5.25VEE121 pKa = 4.24AHH123 pKa = 5.97EE124 pKa = 4.68QGQGDD129 pKa = 3.76IPTFYY134 pKa = 10.0TYY136 pKa = 11.09EE137 pKa = 4.17EE138 pKa = 4.62GLSHH142 pKa = 6.9LTAEE146 pKa = 4.71GQATLEE152 pKa = 4.11RR153 pKa = 11.84LEE155 pKa = 4.74GMLAQSVSSQYY166 pKa = 11.66NMAGVRR172 pKa = 11.84TEE174 pKa = 4.15DD175 pKa = 3.91SIRR178 pKa = 11.84DD179 pKa = 3.72YY180 pKa = 11.37EE181 pKa = 5.31DD182 pKa = 3.35GMEE185 pKa = 4.19IDD187 pKa = 4.27AAPTVAGQFEE197 pKa = 4.58DD198 pKa = 4.85ADD200 pKa = 3.71VDD202 pKa = 3.98HH203 pKa = 7.2

Molecular weight: 22.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q2AB85|Q2AB85_MONDO Taste receptor type 2 OS=Monodelphis domestica OX=13616 GN=TAS2R60 PE=3 SV=1
MM1 pKa = 7.13ARR3 pKa = 11.84YY4 pKa = 9.14RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84SRR9 pKa = 11.84SRR11 pKa = 11.84SRR13 pKa = 11.84SRR15 pKa = 11.84YY16 pKa = 6.77GRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84SRR24 pKa = 11.84SRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84SRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84RR40 pKa = 11.84GRR42 pKa = 11.84GYY44 pKa = 10.41HH45 pKa = 5.72RR46 pKa = 11.84RR47 pKa = 11.84SPHH50 pKa = 4.72RR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 3.2

Molecular weight: 7.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

21225

14996

36221

22459468

16

8347

620.1

69.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.221 ± 0.01

2.22 ± 0.011

4.785 ± 0.008

7.165 ± 0.016

3.855 ± 0.008

6.179 ± 0.014

2.611 ± 0.007

5.034 ± 0.011

6.253 ± 0.016

9.83 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.005

4.045 ± 0.008

5.832 ± 0.017

4.787 ± 0.011

5.35 ± 0.011

8.397 ± 0.019

5.44 ± 0.009

5.734 ± 0.012

1.201 ± 0.005

2.785 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski