Pectobacterium phage My1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Demerecviridae; Mccorquodalevirinae; Myunavirus; Pectobacterium virus My1

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 149 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J9QPU1|J9QPU1_9CAUD Uncharacterized protein OS=Pectobacterium phage My1 OX=1204539 GN=My1_067 PE=4 SV=1
MM1 pKa = 8.37DD2 pKa = 4.61IMTTPAINLLGVGLFSVTIYY22 pKa = 9.81RR23 pKa = 11.84TDD25 pKa = 3.51DD26 pKa = 3.51SEE28 pKa = 4.44DD29 pKa = 3.66TVTVIVPEE37 pKa = 4.24FFLEE41 pKa = 4.22KK42 pKa = 10.37FFEE45 pKa = 4.2EE46 pKa = 4.62FAQFKK51 pKa = 10.75EE52 pKa = 4.09EE53 pKa = 3.83LTAYY57 pKa = 11.06SDD59 pKa = 4.01MEE61 pKa = 4.98DD62 pKa = 3.55LAAMYY67 pKa = 7.15PTVYY71 pKa = 10.29GYY73 pKa = 11.04VFEE76 pKa = 4.48EE77 pKa = 4.71QGFDD81 pKa = 3.54LNKK84 pKa = 10.32AEE86 pKa = 5.23HH87 pKa = 7.22IEE89 pKa = 4.09IQWGISFEE97 pKa = 4.35VGSPFPRR104 pKa = 11.84YY105 pKa = 9.66FEE107 pKa = 4.15GLEE110 pKa = 3.83II111 pKa = 5.29

Molecular weight: 12.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J9QKZ0|J9QKZ0_9CAUD Uncharacterized protein OS=Pectobacterium phage My1 OX=1204539 GN=My1_065 PE=4 SV=1
MM1 pKa = 7.63RR2 pKa = 11.84RR3 pKa = 11.84KK4 pKa = 9.98LKK6 pKa = 10.14RR7 pKa = 11.84KK8 pKa = 8.39YY9 pKa = 9.62IRR11 pKa = 11.84RR12 pKa = 11.84INSLRR17 pKa = 11.84EE18 pKa = 3.88YY19 pKa = 11.04AKK21 pKa = 10.47GWPLQILIADD31 pKa = 4.09RR32 pKa = 11.84RR33 pKa = 11.84PRR35 pKa = 11.84YY36 pKa = 9.58NEE38 pKa = 3.21EE39 pKa = 3.61FIYY42 pKa = 10.8YY43 pKa = 9.78RR44 pKa = 11.84DD45 pKa = 3.18WDD47 pKa = 3.91KK48 pKa = 11.72YY49 pKa = 10.85CRR51 pKa = 11.84TKK53 pKa = 10.53QGRR56 pKa = 11.84LSGAYY61 pKa = 9.03EE62 pKa = 3.66EE63 pKa = 4.83HH64 pKa = 6.79FGVKK68 pKa = 9.91PSRR71 pKa = 11.84LAVGNKK77 pKa = 9.27RR78 pKa = 11.84LAA80 pKa = 3.67

Molecular weight: 9.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

149

0

149

30586

46

1435

205.3

22.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.948 ± 0.689

1.04 ± 0.084

5.728 ± 0.197

6.774 ± 0.227

3.786 ± 0.174

6.644 ± 0.219

1.779 ± 0.136

6.568 ± 0.189

6.219 ± 0.254

8.671 ± 0.342

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.119

4.914 ± 0.143

3.56 ± 0.178

3.423 ± 0.177

4.463 ± 0.189

7.337 ± 0.296

6.836 ± 0.434

6.853 ± 0.183

1.138 ± 0.091

3.999 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski