Amino acid dipepetide frequency for Pectobacterium phage My1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.716AlaAla: 7.716 ± 1.822
0.654AlaCys: 0.654 ± 0.144
3.335AlaAsp: 3.335 ± 0.322
5.362AlaGlu: 5.362 ± 0.529
2.06AlaPhe: 2.06 ± 0.233
4.316AlaGly: 4.316 ± 0.505
1.373AlaHis: 1.373 ± 0.219
5.166AlaIle: 5.166 ± 0.406
6.016AlaLys: 6.016 ± 0.501
7.291AlaLeu: 7.291 ± 0.471
2.223AlaMet: 2.223 ± 0.29
3.597AlaAsn: 3.597 ± 0.495
2.223AlaPro: 2.223 ± 0.392
3.073AlaGln: 3.073 ± 0.472
3.564AlaArg: 3.564 ± 0.302
6.31AlaSer: 6.31 ± 1.46
6.833AlaThr: 6.833 ± 2.097
4.675AlaVal: 4.675 ± 0.374
0.817AlaTrp: 0.817 ± 0.207
2.877AlaTyr: 2.877 ± 0.311
0.0AlaXaa: 0.0 ± 0.0
Cys
0.621CysAla: 0.621 ± 0.158
0.196CysCys: 0.196 ± 0.08
0.589CysAsp: 0.589 ± 0.154
0.523CysGlu: 0.523 ± 0.171
0.589CysPhe: 0.589 ± 0.133
0.785CysGly: 0.785 ± 0.207
0.294CysHis: 0.294 ± 0.095
0.621CysIle: 0.621 ± 0.16
0.687CysLys: 0.687 ± 0.133
0.687CysLeu: 0.687 ± 0.162
0.196CysMet: 0.196 ± 0.077
0.49CysAsn: 0.49 ± 0.133
0.49CysPro: 0.49 ± 0.138
0.425CysGln: 0.425 ± 0.15
0.36CysArg: 0.36 ± 0.096
0.719CysSer: 0.719 ± 0.131
0.752CysThr: 0.752 ± 0.157
0.948CysVal: 0.948 ± 0.181
0.033CysTrp: 0.033 ± 0.037
0.392CysTyr: 0.392 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
4.512AspAla: 4.512 ± 0.397
0.49AspCys: 0.49 ± 0.143
2.714AspAsp: 2.714 ± 0.394
4.12AspGlu: 4.12 ± 0.351
1.929AspPhe: 1.929 ± 0.289
3.891AspGly: 3.891 ± 0.552
0.883AspHis: 0.883 ± 0.238
4.152AspIle: 4.152 ± 0.436
3.564AspLys: 3.564 ± 0.385
5.002AspLeu: 5.002 ± 0.576
1.667AspMet: 1.667 ± 0.222
2.387AspAsn: 2.387 ± 0.299
2.027AspPro: 2.027 ± 0.239
1.373AspGln: 1.373 ± 0.294
2.289AspArg: 2.289 ± 0.322
4.218AspSer: 4.218 ± 0.432
4.087AspThr: 4.087 ± 0.339
4.054AspVal: 4.054 ± 0.37
0.883AspTrp: 0.883 ± 0.201
3.041AspTyr: 3.041 ± 0.308
0.0AspXaa: 0.0 ± 0.0
Glu
6.441GluAla: 6.441 ± 0.565
0.981GluCys: 0.981 ± 0.211
3.956GluAsp: 3.956 ± 0.382
4.675GluGlu: 4.675 ± 0.552
2.518GluPhe: 2.518 ± 0.303
4.087GluGly: 4.087 ± 0.332
1.504GluHis: 1.504 ± 0.221
4.806GluIle: 4.806 ± 0.48
4.054GluLys: 4.054 ± 0.42
6.964GluLeu: 6.964 ± 0.54
1.373GluMet: 1.373 ± 0.198
2.616GluAsn: 2.616 ± 0.334
1.798GluPro: 1.798 ± 0.312
2.681GluGln: 2.681 ± 0.334
2.289GluArg: 2.289 ± 0.249
4.283GluSer: 4.283 ± 0.414
3.531GluThr: 3.531 ± 0.338
4.806GluVal: 4.806 ± 0.406
0.785GluTrp: 0.785 ± 0.154
3.597GluTyr: 3.597 ± 0.369
0.0GluXaa: 0.0 ± 0.0
Phe
2.06PheAla: 2.06 ± 0.226
0.392PheCys: 0.392 ± 0.131
2.975PheAsp: 2.975 ± 0.384
2.223PheGlu: 2.223 ± 0.363
1.341PhePhe: 1.341 ± 0.234
2.583PheGly: 2.583 ± 0.357
0.85PheHis: 0.85 ± 0.192
2.485PheIle: 2.485 ± 0.317
2.648PheLys: 2.648 ± 0.381
3.073PheLeu: 3.073 ± 0.402
0.915PheMet: 0.915 ± 0.183
2.681PheAsn: 2.681 ± 0.295
1.21PhePro: 1.21 ± 0.212
0.948PheGln: 0.948 ± 0.157
1.471PheArg: 1.471 ± 0.243
3.302PheSer: 3.302 ± 0.38
2.681PheThr: 2.681 ± 0.333
2.419PheVal: 2.419 ± 0.339
0.392PheTrp: 0.392 ± 0.127
1.21PheTyr: 1.21 ± 0.182
0.0PheXaa: 0.0 ± 0.0
Gly
5.068GlyAla: 5.068 ± 0.689
0.981GlyCys: 0.981 ± 0.211
4.479GlyAsp: 4.479 ± 0.395
4.316GlyGlu: 4.316 ± 0.285
3.4GlyPhe: 3.4 ± 0.297
3.466GlyGly: 3.466 ± 0.425
0.883GlyHis: 0.883 ± 0.191
4.97GlyIle: 4.97 ± 0.447
4.806GlyLys: 4.806 ± 0.462
4.741GlyLeu: 4.741 ± 0.471
1.144GlyMet: 1.144 ± 0.173
3.106GlyAsn: 3.106 ± 0.36
1.144GlyPro: 1.144 ± 0.218
1.994GlyGln: 1.994 ± 0.28
2.714GlyArg: 2.714 ± 0.249
4.774GlySer: 4.774 ± 0.404
5.035GlyThr: 5.035 ± 0.572
5.526GlyVal: 5.526 ± 0.35
1.046GlyTrp: 1.046 ± 0.189
2.845GlyTyr: 2.845 ± 0.395
0.0GlyXaa: 0.0 ± 0.0
His
0.85HisAla: 0.85 ± 0.185
0.327HisCys: 0.327 ± 0.117
1.046HisAsp: 1.046 ± 0.209
0.752HisGlu: 0.752 ± 0.168
0.621HisPhe: 0.621 ± 0.155
1.341HisGly: 1.341 ± 0.201
0.392HisHis: 0.392 ± 0.144
1.373HisIle: 1.373 ± 0.213
0.915HisLys: 0.915 ± 0.191
1.635HisLeu: 1.635 ± 0.255
0.458HisMet: 0.458 ± 0.143
0.883HisAsn: 0.883 ± 0.209
0.817HisPro: 0.817 ± 0.188
0.49HisGln: 0.49 ± 0.149
1.014HisArg: 1.014 ± 0.212
1.242HisSer: 1.242 ± 0.213
1.144HisThr: 1.144 ± 0.201
1.275HisVal: 1.275 ± 0.206
0.36HisTrp: 0.36 ± 0.099
0.85HisTyr: 0.85 ± 0.144
0.0HisXaa: 0.0 ± 0.0
Ile
5.068IleAla: 5.068 ± 0.448
0.654IleCys: 0.654 ± 0.162
4.479IleAsp: 4.479 ± 0.387
5.101IleGlu: 5.101 ± 0.527
1.994IlePhe: 1.994 ± 0.29
4.25IleGly: 4.25 ± 0.484
0.981IleHis: 0.981 ± 0.223
3.727IleIle: 3.727 ± 0.337
4.022IleLys: 4.022 ± 0.41
5.722IleLeu: 5.722 ± 0.478
1.471IleMet: 1.471 ± 0.247
3.368IleAsn: 3.368 ± 0.316
3.433IlePro: 3.433 ± 0.418
2.648IleGln: 2.648 ± 0.298
2.452IleArg: 2.452 ± 0.293
4.708IleSer: 4.708 ± 0.367
5.427IleThr: 5.427 ± 0.703
3.597IleVal: 3.597 ± 0.329
0.49IleTrp: 0.49 ± 0.125
2.06IleTyr: 2.06 ± 0.258
0.0IleXaa: 0.0 ± 0.0
Lys
5.885LysAla: 5.885 ± 0.6
0.556LysCys: 0.556 ± 0.133
3.597LysAsp: 3.597 ± 0.338
4.872LysGlu: 4.872 ± 0.435
2.485LysPhe: 2.485 ± 0.279
4.152LysGly: 4.152 ± 0.397
1.177LysHis: 1.177 ± 0.182
3.073LysIle: 3.073 ± 0.329
3.466LysLys: 3.466 ± 0.408
5.918LysLeu: 5.918 ± 0.63
1.798LysMet: 1.798 ± 0.249
2.681LysAsn: 2.681 ± 0.259
2.518LysPro: 2.518 ± 0.319
2.125LysGln: 2.125 ± 0.301
2.714LysArg: 2.714 ± 0.293
4.12LysSer: 4.12 ± 0.428
3.4LysThr: 3.4 ± 0.445
4.283LysVal: 4.283 ± 0.376
0.621LysTrp: 0.621 ± 0.158
2.746LysTyr: 2.746 ± 0.288
0.0LysXaa: 0.0 ± 0.0
Leu
6.866LeuAla: 6.866 ± 0.446
0.915LeuCys: 0.915 ± 0.174
5.853LeuAsp: 5.853 ± 0.547
7.749LeuGlu: 7.749 ± 0.519
2.681LeuPhe: 2.681 ± 0.311
6.212LeuGly: 6.212 ± 0.454
1.602LeuHis: 1.602 ± 0.248
4.512LeuIle: 4.512 ± 0.397
5.395LeuLys: 5.395 ± 0.527
7.324LeuLeu: 7.324 ± 0.663
1.962LeuMet: 1.962 ± 0.28
4.741LeuAsn: 4.741 ± 0.546
3.237LeuPro: 3.237 ± 0.409
3.597LeuGln: 3.597 ± 0.303
4.414LeuArg: 4.414 ± 0.449
5.951LeuSer: 5.951 ± 0.469
4.937LeuThr: 4.937 ± 0.47
5.362LeuVal: 5.362 ± 0.448
0.589LeuTrp: 0.589 ± 0.143
2.812LeuTyr: 2.812 ± 0.304
0.0LeuXaa: 0.0 ± 0.0
Met
1.733MetAla: 1.733 ± 0.188
0.196MetCys: 0.196 ± 0.076
1.046MetAsp: 1.046 ± 0.188
1.406MetGlu: 1.406 ± 0.247
0.85MetPhe: 0.85 ± 0.143
1.602MetGly: 1.602 ± 0.181
0.49MetHis: 0.49 ± 0.112
1.439MetIle: 1.439 ± 0.199
1.7MetLys: 1.7 ± 0.297
2.158MetLeu: 2.158 ± 0.272
0.392MetMet: 0.392 ± 0.107
1.112MetAsn: 1.112 ± 0.209
1.177MetPro: 1.177 ± 0.196
1.046MetGln: 1.046 ± 0.227
1.112MetArg: 1.112 ± 0.206
1.864MetSer: 1.864 ± 0.245
1.635MetThr: 1.635 ± 0.228
1.308MetVal: 1.308 ± 0.188
0.229MetTrp: 0.229 ± 0.081
0.719MetTyr: 0.719 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
3.498AsnAla: 3.498 ± 0.518
0.425AsnCys: 0.425 ± 0.119
2.093AsnAsp: 2.093 ± 0.308
3.106AsnGlu: 3.106 ± 0.334
1.798AsnPhe: 1.798 ± 0.333
4.152AsnGly: 4.152 ± 0.386
0.654AsnHis: 0.654 ± 0.119
3.4AsnIle: 3.4 ± 0.347
2.845AsnLys: 2.845 ± 0.348
4.022AsnLeu: 4.022 ± 0.386
1.275AsnMet: 1.275 ± 0.217
2.583AsnAsn: 2.583 ± 0.292
2.387AsnPro: 2.387 ± 0.306
1.766AsnGln: 1.766 ± 0.247
2.55AsnArg: 2.55 ± 0.325
4.022AsnSer: 4.022 ± 0.452
3.008AsnThr: 3.008 ± 0.341
3.008AsnVal: 3.008 ± 0.302
0.948AsnTrp: 0.948 ± 0.211
1.602AsnTyr: 1.602 ± 0.226
0.0AsnXaa: 0.0 ± 0.0
Pro
2.583ProAla: 2.583 ± 0.271
0.425ProCys: 0.425 ± 0.139
2.125ProAsp: 2.125 ± 0.303
2.779ProGlu: 2.779 ± 0.347
1.766ProPhe: 1.766 ± 0.193
1.896ProGly: 1.896 ± 0.288
0.817ProHis: 0.817 ± 0.194
2.191ProIle: 2.191 ± 0.271
2.321ProLys: 2.321 ± 0.306
2.223ProLeu: 2.223 ± 0.249
0.719ProMet: 0.719 ± 0.164
2.027ProAsn: 2.027 ± 0.272
1.242ProPro: 1.242 ± 0.283
0.915ProGln: 0.915 ± 0.19
1.864ProArg: 1.864 ± 0.284
2.877ProSer: 2.877 ± 0.329
2.321ProThr: 2.321 ± 0.247
2.583ProVal: 2.583 ± 0.292
0.49ProTrp: 0.49 ± 0.116
1.439ProTyr: 1.439 ± 0.209
0.0ProXaa: 0.0 ± 0.0
Gln
2.845GlnAla: 2.845 ± 0.633
0.36GlnCys: 0.36 ± 0.117
2.093GlnAsp: 2.093 ± 0.31
2.256GlnGlu: 2.256 ± 0.292
1.373GlnPhe: 1.373 ± 0.161
1.733GlnGly: 1.733 ± 0.239
0.687GlnHis: 0.687 ± 0.16
2.518GlnIle: 2.518 ± 0.303
1.864GlnLys: 1.864 ± 0.305
3.498GlnLeu: 3.498 ± 0.387
0.719GlnMet: 0.719 ± 0.14
1.504GlnAsn: 1.504 ± 0.228
0.915GlnPro: 0.915 ± 0.19
1.733GlnGln: 1.733 ± 0.217
1.733GlnArg: 1.733 ± 0.234
2.289GlnSer: 2.289 ± 0.287
1.798GlnThr: 1.798 ± 0.286
2.223GlnVal: 2.223 ± 0.288
0.458GlnTrp: 0.458 ± 0.117
1.635GlnTyr: 1.635 ± 0.288
0.0GlnXaa: 0.0 ± 0.0
Arg
3.139ArgAla: 3.139 ± 0.271
0.098ArgCys: 0.098 ± 0.051
2.583ArgAsp: 2.583 ± 0.294
2.91ArgGlu: 2.91 ± 0.358
1.602ArgPhe: 1.602 ± 0.259
3.139ArgGly: 3.139 ± 0.307
0.556ArgHis: 0.556 ± 0.145
3.171ArgIle: 3.171 ± 0.366
2.877ArgLys: 2.877 ± 0.33
3.76ArgLeu: 3.76 ± 0.395
1.112ArgMet: 1.112 ± 0.176
2.648ArgAsn: 2.648 ± 0.332
1.406ArgPro: 1.406 ± 0.244
1.537ArgGln: 1.537 ± 0.23
2.877ArgArg: 2.877 ± 0.412
2.714ArgSer: 2.714 ± 0.27
2.812ArgThr: 2.812 ± 0.289
3.466ArgVal: 3.466 ± 0.314
0.392ArgTrp: 0.392 ± 0.108
1.831ArgTyr: 1.831 ± 0.254
0.0ArgXaa: 0.0 ± 0.0
Ser
6.245SerAla: 6.245 ± 1.573
0.687SerCys: 0.687 ± 0.141
3.368SerAsp: 3.368 ± 0.327
3.989SerGlu: 3.989 ± 0.438
3.727SerPhe: 3.727 ± 0.519
5.362SerGly: 5.362 ± 0.402
0.915SerHis: 0.915 ± 0.183
5.885SerIle: 5.885 ± 0.491
4.087SerLys: 4.087 ± 0.443
6.147SerLeu: 6.147 ± 0.54
1.864SerMet: 1.864 ± 0.282
3.335SerAsn: 3.335 ± 0.344
2.158SerPro: 2.158 ± 0.283
1.929SerGln: 1.929 ± 0.29
2.943SerArg: 2.943 ± 0.343
6.31SerSer: 6.31 ± 0.562
5.362SerThr: 5.362 ± 0.628
5.82SerVal: 5.82 ± 0.478
0.883SerTrp: 0.883 ± 0.14
2.354SerTyr: 2.354 ± 0.284
0.0SerXaa: 0.0 ± 0.0
Thr
5.656ThrAla: 5.656 ± 1.435
0.556ThrCys: 0.556 ± 0.119
3.531ThrAsp: 3.531 ± 0.352
4.152ThrGlu: 4.152 ± 0.407
2.321ThrPhe: 2.321 ± 0.251
6.081ThrGly: 6.081 ± 0.714
1.046ThrHis: 1.046 ± 0.166
4.839ThrIle: 4.839 ± 0.399
4.12ThrLys: 4.12 ± 0.464
6.049ThrLeu: 6.049 ± 0.492
1.373ThrMet: 1.373 ± 0.21
3.629ThrAsn: 3.629 ± 0.3
2.55ThrPro: 2.55 ± 0.26
2.027ThrGln: 2.027 ± 0.279
2.419ThrArg: 2.419 ± 0.289
4.97ThrSer: 4.97 ± 0.797
5.264ThrThr: 5.264 ± 1.145
4.675ThrVal: 4.675 ± 0.348
0.752ThrTrp: 0.752 ± 0.184
2.354ThrTyr: 2.354 ± 0.259
0.0ThrXaa: 0.0 ± 0.0
Val
5.166ValAla: 5.166 ± 0.432
0.589ValCys: 0.589 ± 0.134
4.218ValAsp: 4.218 ± 0.399
4.185ValGlu: 4.185 ± 0.404
2.616ValPhe: 2.616 ± 0.284
3.989ValGly: 3.989 ± 0.363
1.439ValHis: 1.439 ± 0.224
4.414ValIle: 4.414 ± 0.43
3.956ValLys: 3.956 ± 0.383
6.31ValLeu: 6.31 ± 0.517
1.667ValMet: 1.667 ± 0.246
3.073ValAsn: 3.073 ± 0.377
2.648ValPro: 2.648 ± 0.361
2.256ValGln: 2.256 ± 0.256
3.335ValArg: 3.335 ± 0.342
5.199ValSer: 5.199 ± 0.438
4.904ValThr: 4.904 ± 0.445
4.839ValVal: 4.839 ± 0.428
0.687ValTrp: 0.687 ± 0.18
3.041ValTyr: 3.041 ± 0.338
0.0ValXaa: 0.0 ± 0.0
Trp
0.556TrpAla: 0.556 ± 0.167
0.294TrpCys: 0.294 ± 0.126
0.817TrpAsp: 0.817 ± 0.18
0.948TrpGlu: 0.948 ± 0.173
0.589TrpPhe: 0.589 ± 0.179
0.752TrpGly: 0.752 ± 0.169
0.262TrpHis: 0.262 ± 0.078
0.523TrpIle: 0.523 ± 0.125
0.425TrpLys: 0.425 ± 0.128
1.014TrpLeu: 1.014 ± 0.187
0.229TrpMet: 0.229 ± 0.092
0.687TrpAsn: 0.687 ± 0.145
0.36TrpPro: 0.36 ± 0.118
0.392TrpGln: 0.392 ± 0.126
0.392TrpArg: 0.392 ± 0.097
0.85TrpSer: 0.85 ± 0.197
0.654TrpThr: 0.654 ± 0.169
0.948TrpVal: 0.948 ± 0.169
0.033TrpTrp: 0.033 ± 0.033
0.654TrpTyr: 0.654 ± 0.15
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.975TyrAla: 2.975 ± 0.328
0.621TyrCys: 0.621 ± 0.135
2.387TyrAsp: 2.387 ± 0.279
2.321TyrGlu: 2.321 ± 0.313
1.602TyrPhe: 1.602 ± 0.213
2.648TyrGly: 2.648 ± 0.306
0.981TyrHis: 0.981 ± 0.183
2.419TyrIle: 2.419 ± 0.312
2.419TyrLys: 2.419 ± 0.301
3.171TyrLeu: 3.171 ± 0.363
0.621TyrMet: 0.621 ± 0.155
1.994TyrAsn: 1.994 ± 0.186
1.864TyrPro: 1.864 ± 0.265
1.275TyrGln: 1.275 ± 0.207
2.125TyrArg: 2.125 ± 0.334
2.648TyrSer: 2.648 ± 0.3
2.779TyrThr: 2.779 ± 0.281
2.714TyrVal: 2.714 ± 0.237
0.49TyrTrp: 0.49 ± 0.128
1.929TyrTyr: 1.929 ± 0.295
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 149 proteins (30586 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski