Lonsdalea quercina
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3284 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H3VJ85|A0A1H3VJ85_9GAMM Flagellar assembly protein FliH OS=Lonsdalea quercina OX=71657 GN=SAMN02982996_00018 PE=3 SV=1
MM1 pKa = 7.53 GFSQAVSGLNAASSNLDD18 pKa = 3.36 VIGNNIANSEE28 pKa = 4.2 TTGFKK33 pKa = 10.66 GATVSFADD41 pKa = 3.81 IFADD45 pKa = 3.63 SAVGLGVKK53 pKa = 9.71 VAAVTQNFNDD63 pKa = 4.04 GTIEE67 pKa = 4.04 NTDD70 pKa = 3.01 VGTDD74 pKa = 3.43 VAISGNGFYY83 pKa = 10.37 RR84 pKa = 11.84 VADD87 pKa = 4.01 DD88 pKa = 4.61 AGSVYY93 pKa = 7.96 YY94 pKa = 9.92 TRR96 pKa = 11.84 NGEE99 pKa = 4.13 FQLDD103 pKa = 3.44 ADD105 pKa = 4.82 RR106 pKa = 11.84 NLTTKK111 pKa = 9.0 TGYY114 pKa = 10.73 YY115 pKa = 7.2 VTGYY119 pKa = 9.82 SATGTPPTIAAGAEE133 pKa = 3.89 PTTINISAGSIAAQQTSTSTYY154 pKa = 9.19 VANLNSTTDD163 pKa = 3.31 ALAAGTTFDD172 pKa = 3.99 ATDD175 pKa = 3.08 SSTYY179 pKa = 10.64 SWTKK183 pKa = 11.22 SMTTYY188 pKa = 11.22 DD189 pKa = 3.7 SLGNTHH195 pKa = 6.18 VMNLYY200 pKa = 10.18 FSKK203 pKa = 9.71 TADD206 pKa = 3.63 NTWEE210 pKa = 3.93 VHH212 pKa = 6.57 ALDD215 pKa = 5.07 SSDD218 pKa = 3.66 STATAQDD225 pKa = 3.8 LGTLNFSTSGQLTGTTSFNVTTNSLNGSAANTFTIDD261 pKa = 3.63 FAGSTQQNASSSEE274 pKa = 3.85 LSKK277 pKa = 9.39 TQNGYY282 pKa = 5.41 TTGNLVEE289 pKa = 4.25 YY290 pKa = 10.27 AINDD294 pKa = 3.76 DD295 pKa = 3.69 GTINGTYY302 pKa = 10.68 SNGQTQLLGQIVLATFSNNQGLKK325 pKa = 10.24 SEE327 pKa = 4.53 GDD329 pKa = 4.02 NVWSATSSSGEE340 pKa = 3.9 ASLGTAGTGLLGSLTSGALEE360 pKa = 4.04 SSNVDD365 pKa = 3.13 VGSEE369 pKa = 4.06 LVNMIVAQRR378 pKa = 11.84 NYY380 pKa = 10.23 QSNAQTIKK388 pKa = 9.41 TQDD391 pKa = 3.56 SILNTLVNLRR401 pKa = 4.14
Molecular weight: 41.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.897
IPC_protein 3.91
Toseland 3.681
ProMoST 4.075
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.846
Rodwell 3.732
Grimsley 3.592
Solomon 3.897
Lehninger 3.846
Nozaki 4.012
DTASelect 4.279
Thurlkill 3.732
EMBOSS 3.859
Sillero 4.024
Patrickios 1.926
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.913
Protein with the highest isoelectric point:
>tr|A0A1H4CKW3|A0A1H4CKW3_9GAMM Transcriptional regulator LacI family OS=Lonsdalea quercina OX=71657 GN=SAMN02982996_02012 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.6 RR12 pKa = 11.84 NRR14 pKa = 11.84 SHH16 pKa = 7.16 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.25 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LSVSKK46 pKa = 10.99
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3284
0
3284
1066459
40
3730
324.7
35.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.73 ± 0.045
1.052 ± 0.015
5.344 ± 0.032
5.574 ± 0.038
3.663 ± 0.023
7.335 ± 0.038
2.347 ± 0.023
5.553 ± 0.037
3.787 ± 0.04
11.124 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.62 ± 0.021
3.529 ± 0.033
4.621 ± 0.03
4.737 ± 0.038
6.107 ± 0.045
6.23 ± 0.044
5.392 ± 0.029
7.084 ± 0.031
1.373 ± 0.016
2.799 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here