Microbacterium phage FuzzBuster

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 85 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A516KV06|A0A516KV06_9CAUD Uncharacterized protein OS=Microbacterium phage FuzzBuster OX=2590935 GN=30 PE=4 SV=1
MM1 pKa = 7.41ATHH4 pKa = 6.89CFIPVLGKK12 pKa = 10.22RR13 pKa = 11.84LRR15 pKa = 11.84ATEE18 pKa = 4.3LDD20 pKa = 3.16ACGVVTPSTSLEE32 pKa = 3.99VTTDD36 pKa = 3.13GFITITLSSEE46 pKa = 4.1VEE48 pKa = 4.11EE49 pKa = 4.49GTEE52 pKa = 3.62ILQRR56 pKa = 11.84NAAGQLCVNQKK67 pKa = 9.82QADD70 pKa = 3.74SFKK73 pKa = 10.6RR74 pKa = 11.84FTVEE78 pKa = 4.88IEE80 pKa = 4.1FCGVNPSLLSIVSNAEE96 pKa = 3.6PYY98 pKa = 10.39EE99 pKa = 4.58DD100 pKa = 3.78YY101 pKa = 11.32AGDD104 pKa = 3.53VAGFTVPEE112 pKa = 4.14GEE114 pKa = 4.11ITKK117 pKa = 10.46KK118 pKa = 10.26FALEE122 pKa = 3.88LWTGLSGQACLPGEE136 pKa = 4.49AQDD139 pKa = 3.78EE140 pKa = 4.46ASGYY144 pKa = 8.84MLLPFVQAGVLGDD157 pKa = 3.39ITIDD161 pKa = 3.51GEE163 pKa = 4.32NAVTFSITGAYY174 pKa = 8.5TIGGNAWLNGPYY186 pKa = 10.15NVLRR190 pKa = 11.84NVEE193 pKa = 4.13GDD195 pKa = 3.7PAVLPLALDD204 pKa = 4.09PFDD207 pKa = 5.2HH208 pKa = 6.94LLLVDD213 pKa = 4.13TALAPPPVACSPTAVAPLPPVAATGAIAGNPGTFQPGGATVPANLAAMASLTASPNTAWTTGQHH277 pKa = 5.07VVLGDD282 pKa = 3.48ASHH285 pKa = 7.05AYY287 pKa = 9.02WDD289 pKa = 3.99GDD291 pKa = 3.12SWAAGDD297 pKa = 4.21APP299 pKa = 4.27

Molecular weight: 30.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A516KV25|A0A516KV25_9CAUD Uncharacterized protein OS=Microbacterium phage FuzzBuster OX=2590935 GN=59 PE=4 SV=1
MM1 pKa = 7.26MSKK4 pKa = 8.48TCEE7 pKa = 3.93TGKK10 pKa = 9.93VRR12 pKa = 11.84HH13 pKa = 5.95TSLARR18 pKa = 11.84AKK20 pKa = 9.02TAAHH24 pKa = 6.43AFARR28 pKa = 11.84EE29 pKa = 3.9LNRR32 pKa = 11.84EE33 pKa = 3.6KK34 pKa = 10.75RR35 pKa = 11.84IAQNMYY41 pKa = 10.1AYY43 pKa = 10.2RR44 pKa = 11.84CSTCRR49 pKa = 11.84GWHH52 pKa = 5.61LTRR55 pKa = 11.84QAGWSNVTLVMQAAPEE71 pKa = 4.08SLQRR75 pKa = 11.84WAMDD79 pKa = 3.32GG80 pKa = 3.41

Molecular weight: 9.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

85

0

85

17508

39

931

206.0

22.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.68 ± 0.378

0.937 ± 0.138

6.271 ± 0.29

6.963 ± 0.377

2.565 ± 0.134

8.625 ± 0.267

1.948 ± 0.166

4.963 ± 0.252

3.456 ± 0.388

7.716 ± 0.399

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.21 ± 0.123

2.445 ± 0.169

5.826 ± 0.232

3.061 ± 0.145

7.18 ± 0.476

5.426 ± 0.253

6.785 ± 0.255

6.98 ± 0.214

1.902 ± 0.155

2.062 ± 0.169
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski