Amino acid dipepetide frequency for Microbacterium phage FuzzBuster

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.508AlaAla: 16.508 ± 1.949
0.914AlaCys: 0.914 ± 0.325
6.169AlaAsp: 6.169 ± 0.602
9.196AlaGlu: 9.196 ± 0.988
2.628AlaPhe: 2.628 ± 0.342
11.024AlaGly: 11.024 ± 0.926
2.342AlaHis: 2.342 ± 0.465
7.083AlaIle: 7.083 ± 0.755
5.312AlaLys: 5.312 ± 0.83
11.71AlaLeu: 11.71 ± 1.272
3.427AlaMet: 3.427 ± 0.42
3.37AlaAsn: 3.37 ± 0.54
6.912AlaPro: 6.912 ± 0.559
4.056AlaGln: 4.056 ± 0.465
9.139AlaArg: 9.139 ± 0.903
6.34AlaSer: 6.34 ± 0.703
7.768AlaThr: 7.768 ± 0.613
8.054AlaVal: 8.054 ± 0.779
2.856AlaTrp: 2.856 ± 0.402
1.942AlaTyr: 1.942 ± 0.291
0.0AlaXaa: 0.0 ± 0.0
Cys
1.085CysAla: 1.085 ± 0.338
0.057CysCys: 0.057 ± 0.056
0.685CysAsp: 0.685 ± 0.181
0.914CysGlu: 0.914 ± 0.278
0.286CysPhe: 0.286 ± 0.114
1.428CysGly: 1.428 ± 0.338
0.114CysHis: 0.114 ± 0.07
0.286CysIle: 0.286 ± 0.13
0.4CysLys: 0.4 ± 0.181
0.286CysLeu: 0.286 ± 0.113
0.057CysMet: 0.057 ± 0.067
0.343CysAsn: 0.343 ± 0.159
0.743CysPro: 0.743 ± 0.208
0.4CysGln: 0.4 ± 0.18
0.514CysArg: 0.514 ± 0.168
0.571CysSer: 0.571 ± 0.198
0.343CysThr: 0.343 ± 0.147
0.4CysVal: 0.4 ± 0.167
0.228CysTrp: 0.228 ± 0.113
0.228CysTyr: 0.228 ± 0.109
0.0CysXaa: 0.0 ± 0.0
Asp
8.34AspAla: 8.34 ± 0.674
0.571AspCys: 0.571 ± 0.267
5.369AspAsp: 5.369 ± 0.746
5.141AspGlu: 5.141 ± 0.54
1.885AspPhe: 1.885 ± 0.279
6.74AspGly: 6.74 ± 0.655
1.142AspHis: 1.142 ± 0.306
2.799AspIle: 2.799 ± 0.33
1.599AspLys: 1.599 ± 0.345
4.227AspLeu: 4.227 ± 0.479
0.743AspMet: 0.743 ± 0.187
1.142AspAsn: 1.142 ± 0.284
4.855AspPro: 4.855 ± 0.643
2.285AspGln: 2.285 ± 0.341
4.056AspArg: 4.056 ± 0.565
2.285AspSer: 2.285 ± 0.365
3.484AspThr: 3.484 ± 0.357
4.17AspVal: 4.17 ± 0.416
0.857AspTrp: 0.857 ± 0.22
1.028AspTyr: 1.028 ± 0.277
0.0AspXaa: 0.0 ± 0.0
Glu
8.625GluAla: 8.625 ± 0.799
0.8GluCys: 0.8 ± 0.249
3.142GluAsp: 3.142 ± 0.594
6.34GluGlu: 6.34 ± 0.776
1.371GluPhe: 1.371 ± 0.253
5.084GluGly: 5.084 ± 0.523
1.771GluHis: 1.771 ± 0.396
3.827GluIle: 3.827 ± 0.497
2.799GluLys: 2.799 ± 0.647
5.655GluLeu: 5.655 ± 0.579
2.171GluMet: 2.171 ± 0.321
1.371GluAsn: 1.371 ± 0.265
2.513GluPro: 2.513 ± 0.406
2.628GluGln: 2.628 ± 0.385
6.283GluArg: 6.283 ± 0.833
3.37GluSer: 3.37 ± 0.511
4.17GluThr: 4.17 ± 0.498
4.912GluVal: 4.912 ± 0.595
1.599GluTrp: 1.599 ± 0.363
1.2GluTyr: 1.2 ± 0.262
0.0GluXaa: 0.0 ± 0.0
Phe
3.427PheAla: 3.427 ± 0.422
0.457PheCys: 0.457 ± 0.16
1.999PheAsp: 1.999 ± 0.311
1.999PheGlu: 1.999 ± 0.326
0.457PhePhe: 0.457 ± 0.162
3.142PheGly: 3.142 ± 0.478
0.343PheHis: 0.343 ± 0.161
0.857PheIle: 0.857 ± 0.23
0.4PheLys: 0.4 ± 0.121
1.885PheLeu: 1.885 ± 0.325
0.514PheMet: 0.514 ± 0.19
0.571PheAsn: 0.571 ± 0.173
0.971PhePro: 0.971 ± 0.201
0.628PheGln: 0.628 ± 0.171
1.771PheArg: 1.771 ± 0.265
1.828PheSer: 1.828 ± 0.366
2.171PheThr: 2.171 ± 0.412
1.314PheVal: 1.314 ± 0.338
0.286PheTrp: 0.286 ± 0.148
0.628PheTyr: 0.628 ± 0.225
0.0PheXaa: 0.0 ± 0.0
Gly
9.71GlyAla: 9.71 ± 0.7
1.085GlyCys: 1.085 ± 0.326
5.712GlyAsp: 5.712 ± 0.591
6.397GlyGlu: 6.397 ± 0.708
3.084GlyPhe: 3.084 ± 0.447
6.455GlyGly: 6.455 ± 0.767
2.056GlyHis: 2.056 ± 0.356
4.113GlyIle: 4.113 ± 0.52
4.113GlyLys: 4.113 ± 0.67
5.255GlyLeu: 5.255 ± 0.757
2.57GlyMet: 2.57 ± 0.513
3.142GlyAsn: 3.142 ± 0.506
2.97GlyPro: 2.97 ± 0.426
2.799GlyGln: 2.799 ± 0.358
5.255GlyArg: 5.255 ± 0.624
4.398GlySer: 4.398 ± 0.555
6.34GlyThr: 6.34 ± 0.743
6.112GlyVal: 6.112 ± 0.585
2.628GlyTrp: 2.628 ± 0.41
2.056GlyTyr: 2.056 ± 0.344
0.0GlyXaa: 0.0 ± 0.0
His
1.885HisAla: 1.885 ± 0.368
0.171HisCys: 0.171 ± 0.127
1.314HisAsp: 1.314 ± 0.222
1.371HisGlu: 1.371 ± 0.401
0.343HisPhe: 0.343 ± 0.23
2.228HisGly: 2.228 ± 0.36
0.457HisHis: 0.457 ± 0.184
0.685HisIle: 0.685 ± 0.197
0.4HisLys: 0.4 ± 0.169
2.113HisLeu: 2.113 ± 0.332
0.286HisMet: 0.286 ± 0.138
0.628HisAsn: 0.628 ± 0.188
1.485HisPro: 1.485 ± 0.322
0.4HisGln: 0.4 ± 0.134
1.542HisArg: 1.542 ± 0.304
0.685HisSer: 0.685 ± 0.183
0.914HisThr: 0.914 ± 0.277
1.428HisVal: 1.428 ± 0.311
0.571HisTrp: 0.571 ± 0.174
0.571HisTyr: 0.571 ± 0.178
0.0HisXaa: 0.0 ± 0.0
Ile
6.283IleAla: 6.283 ± 0.606
0.343IleCys: 0.343 ± 0.149
4.512IleAsp: 4.512 ± 0.455
3.941IleGlu: 3.941 ± 0.432
0.971IlePhe: 0.971 ± 0.241
3.599IleGly: 3.599 ± 0.498
1.085IleHis: 1.085 ± 0.244
2.97IleIle: 2.97 ± 0.642
1.028IleLys: 1.028 ± 0.263
2.742IleLeu: 2.742 ± 0.418
0.857IleMet: 0.857 ± 0.177
1.771IleAsn: 1.771 ± 0.327
2.513IlePro: 2.513 ± 0.393
1.257IleGln: 1.257 ± 0.256
3.656IleArg: 3.656 ± 0.527
3.027IleSer: 3.027 ± 0.405
3.37IleThr: 3.37 ± 0.512
4.341IleVal: 4.341 ± 0.661
0.914IleTrp: 0.914 ± 0.247
0.457IleTyr: 0.457 ± 0.171
0.0IleXaa: 0.0 ± 0.0
Lys
5.141LysAla: 5.141 ± 0.86
0.286LysCys: 0.286 ± 0.153
1.314LysAsp: 1.314 ± 0.383
1.2LysGlu: 1.2 ± 0.227
0.685LysPhe: 0.685 ± 0.187
3.084LysGly: 3.084 ± 0.679
0.628LysHis: 0.628 ± 0.217
1.942LysIle: 1.942 ± 0.349
3.084LysLys: 3.084 ± 1.186
2.685LysLeu: 2.685 ± 0.508
0.571LysMet: 0.571 ± 0.174
0.343LysAsn: 0.343 ± 0.139
2.57LysPro: 2.57 ± 0.492
0.628LysGln: 0.628 ± 0.176
2.685LysArg: 2.685 ± 0.443
1.999LysSer: 1.999 ± 0.42
2.513LysThr: 2.513 ± 0.398
2.513LysVal: 2.513 ± 0.51
0.114LysTrp: 0.114 ± 0.08
0.571LysTyr: 0.571 ± 0.195
0.0LysXaa: 0.0 ± 0.0
Leu
10.053LeuAla: 10.053 ± 0.831
0.571LeuCys: 0.571 ± 0.146
6.569LeuAsp: 6.569 ± 0.789
4.17LeuGlu: 4.17 ± 0.496
1.885LeuPhe: 1.885 ± 0.477
7.311LeuGly: 7.311 ± 1.008
1.371LeuHis: 1.371 ± 0.295
3.599LeuIle: 3.599 ± 0.673
1.885LeuLys: 1.885 ± 0.343
6.397LeuLeu: 6.397 ± 0.861
1.028LeuMet: 1.028 ± 0.234
1.485LeuAsn: 1.485 ± 0.291
4.627LeuPro: 4.627 ± 0.533
2.228LeuGln: 2.228 ± 0.447
6.055LeuArg: 6.055 ± 0.628
4.455LeuSer: 4.455 ± 0.582
5.826LeuThr: 5.826 ± 0.595
5.198LeuVal: 5.198 ± 0.508
0.857LeuTrp: 0.857 ± 0.18
1.599LeuTyr: 1.599 ± 0.276
0.0LeuXaa: 0.0 ± 0.0
Met
3.427MetAla: 3.427 ± 0.423
0.114MetCys: 0.114 ± 0.075
0.914MetAsp: 0.914 ± 0.199
1.028MetGlu: 1.028 ± 0.238
0.343MetPhe: 0.343 ± 0.138
1.314MetGly: 1.314 ± 0.278
0.171MetHis: 0.171 ± 0.085
1.314MetIle: 1.314 ± 0.27
0.743MetLys: 0.743 ± 0.167
1.771MetLeu: 1.771 ± 0.313
0.971MetMet: 0.971 ± 0.233
0.685MetAsn: 0.685 ± 0.143
1.257MetPro: 1.257 ± 0.262
0.571MetGln: 0.571 ± 0.208
1.314MetArg: 1.314 ± 0.342
1.371MetSer: 1.371 ± 0.333
2.399MetThr: 2.399 ± 0.457
1.771MetVal: 1.771 ± 0.354
0.171MetTrp: 0.171 ± 0.092
0.457MetTyr: 0.457 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
3.998AsnAla: 3.998 ± 0.476
0.114AsnCys: 0.114 ± 0.076
1.485AsnAsp: 1.485 ± 0.283
1.371AsnGlu: 1.371 ± 0.289
0.971AsnPhe: 0.971 ± 0.213
3.142AsnGly: 3.142 ± 0.412
0.628AsnHis: 0.628 ± 0.151
0.571AsnIle: 0.571 ± 0.213
0.343AsnLys: 0.343 ± 0.127
1.942AsnLeu: 1.942 ± 0.297
0.114AsnMet: 0.114 ± 0.082
0.685AsnAsn: 0.685 ± 0.17
1.714AsnPro: 1.714 ± 0.431
0.628AsnGln: 0.628 ± 0.179
1.314AsnArg: 1.314 ± 0.297
1.314AsnSer: 1.314 ± 0.299
1.257AsnThr: 1.257 ± 0.298
1.828AsnVal: 1.828 ± 0.311
0.343AsnTrp: 0.343 ± 0.12
0.685AsnTyr: 0.685 ± 0.178
0.0AsnXaa: 0.0 ± 0.0
Pro
8.111ProAla: 8.111 ± 0.954
0.343ProCys: 0.343 ± 0.129
3.427ProAsp: 3.427 ± 0.474
4.57ProGlu: 4.57 ± 0.564
1.599ProPhe: 1.599 ± 0.33
3.998ProGly: 3.998 ± 0.534
0.743ProHis: 0.743 ± 0.202
2.913ProIle: 2.913 ± 0.405
2.456ProLys: 2.456 ± 0.494
3.941ProLeu: 3.941 ± 0.489
1.142ProMet: 1.142 ± 0.218
1.314ProAsn: 1.314 ± 0.319
4.455ProPro: 4.455 ± 0.655
2.056ProGln: 2.056 ± 0.467
2.685ProArg: 2.685 ± 0.429
3.37ProSer: 3.37 ± 0.32
4.284ProThr: 4.284 ± 0.443
4.741ProVal: 4.741 ± 0.495
1.028ProTrp: 1.028 ± 0.272
1.085ProTyr: 1.085 ± 0.283
0.0ProXaa: 0.0 ± 0.0
Gln
5.198GlnAla: 5.198 ± 0.546
0.228GlnCys: 0.228 ± 0.108
1.542GlnAsp: 1.542 ± 0.241
1.828GlnGlu: 1.828 ± 0.247
0.514GlnPhe: 0.514 ± 0.199
2.285GlnGly: 2.285 ± 0.354
0.685GlnHis: 0.685 ± 0.174
1.542GlnIle: 1.542 ± 0.273
0.857GlnLys: 0.857 ± 0.202
2.513GlnLeu: 2.513 ± 0.571
0.8GlnMet: 0.8 ± 0.206
0.628GlnAsn: 0.628 ± 0.156
1.142GlnPro: 1.142 ± 0.234
1.028GlnGln: 1.028 ± 0.274
2.628GlnArg: 2.628 ± 0.37
1.828GlnSer: 1.828 ± 0.324
1.656GlnThr: 1.656 ± 0.254
2.513GlnVal: 2.513 ± 0.36
0.743GlnTrp: 0.743 ± 0.225
0.457GlnTyr: 0.457 ± 0.142
0.0GlnXaa: 0.0 ± 0.0
Arg
7.997ArgAla: 7.997 ± 0.745
0.571ArgCys: 0.571 ± 0.203
4.227ArgAsp: 4.227 ± 0.582
5.312ArgGlu: 5.312 ± 0.608
1.999ArgPhe: 1.999 ± 0.295
5.484ArgGly: 5.484 ± 0.885
1.885ArgHis: 1.885 ± 0.394
3.427ArgIle: 3.427 ± 0.492
2.57ArgLys: 2.57 ± 0.381
7.14ArgLeu: 7.14 ± 0.672
1.714ArgMet: 1.714 ± 0.327
1.428ArgAsn: 1.428 ± 0.236
3.199ArgPro: 3.199 ± 0.426
2.742ArgGln: 2.742 ± 0.43
6.569ArgArg: 6.569 ± 0.811
4.684ArgSer: 4.684 ± 0.571
3.484ArgThr: 3.484 ± 0.447
4.284ArgVal: 4.284 ± 0.517
1.428ArgTrp: 1.428 ± 0.325
1.656ArgTyr: 1.656 ± 0.322
0.0ArgXaa: 0.0 ± 0.0
Ser
6.397SerAla: 6.397 ± 0.74
0.457SerCys: 0.457 ± 0.159
2.97SerAsp: 2.97 ± 0.363
2.685SerGlu: 2.685 ± 0.425
1.885SerPhe: 1.885 ± 0.362
5.484SerGly: 5.484 ± 0.579
0.8SerHis: 0.8 ± 0.211
2.913SerIle: 2.913 ± 0.344
1.885SerLys: 1.885 ± 0.361
3.998SerLeu: 3.998 ± 0.565
1.885SerMet: 1.885 ± 0.289
1.428SerAsn: 1.428 ± 0.215
3.37SerPro: 3.37 ± 0.502
1.485SerGln: 1.485 ± 0.247
3.827SerArg: 3.827 ± 0.62
3.256SerSer: 3.256 ± 0.398
3.656SerThr: 3.656 ± 0.427
3.37SerVal: 3.37 ± 0.435
1.257SerTrp: 1.257 ± 0.268
1.257SerTyr: 1.257 ± 0.255
0.0SerXaa: 0.0 ± 0.0
Thr
8.454ThrAla: 8.454 ± 0.8
1.314ThrCys: 1.314 ± 0.385
4.741ThrAsp: 4.741 ± 0.528
3.541ThrGlu: 3.541 ± 0.481
2.113ThrPhe: 2.113 ± 0.306
6.283ThrGly: 6.283 ± 0.593
1.371ThrHis: 1.371 ± 0.25
3.827ThrIle: 3.827 ± 0.561
1.771ThrLys: 1.771 ± 0.322
4.341ThrLeu: 4.341 ± 0.612
1.542ThrMet: 1.542 ± 0.298
1.085ThrAsn: 1.085 ± 0.269
6.626ThrPro: 6.626 ± 0.691
1.542ThrGln: 1.542 ± 0.338
3.998ThrArg: 3.998 ± 0.567
3.027ThrSer: 3.027 ± 0.411
5.369ThrThr: 5.369 ± 0.772
4.398ThrVal: 4.398 ± 0.513
0.971ThrTrp: 0.971 ± 0.259
1.542ThrTyr: 1.542 ± 0.375
0.0ThrXaa: 0.0 ± 0.0
Val
8.225ValAla: 8.225 ± 0.724
0.571ValCys: 0.571 ± 0.194
3.884ValAsp: 3.884 ± 0.565
5.712ValGlu: 5.712 ± 0.586
1.656ValPhe: 1.656 ± 0.313
5.598ValGly: 5.598 ± 0.491
0.857ValHis: 0.857 ± 0.233
3.313ValIle: 3.313 ± 0.447
2.056ValLys: 2.056 ± 0.394
5.312ValLeu: 5.312 ± 0.634
1.028ValMet: 1.028 ± 0.274
2.056ValAsn: 2.056 ± 0.319
4.455ValPro: 4.455 ± 0.512
2.171ValGln: 2.171 ± 0.313
4.741ValArg: 4.741 ± 0.488
3.827ValSer: 3.827 ± 0.553
6.283ValThr: 6.283 ± 0.593
5.084ValVal: 5.084 ± 0.581
1.2ValTrp: 1.2 ± 0.36
1.771ValTyr: 1.771 ± 0.326
0.0ValXaa: 0.0 ± 0.0
Trp
1.828TrpAla: 1.828 ± 0.333
0.286TrpCys: 0.286 ± 0.129
1.028TrpAsp: 1.028 ± 0.251
1.828TrpGlu: 1.828 ± 0.385
0.457TrpPhe: 0.457 ± 0.149
0.971TrpGly: 0.971 ± 0.266
0.628TrpHis: 0.628 ± 0.168
1.028TrpIle: 1.028 ± 0.265
0.343TrpLys: 0.343 ± 0.132
1.771TrpLeu: 1.771 ± 0.257
0.343TrpMet: 0.343 ± 0.141
0.4TrpAsn: 0.4 ± 0.114
0.971TrpPro: 0.971 ± 0.266
0.457TrpGln: 0.457 ± 0.133
1.599TrpArg: 1.599 ± 0.304
1.2TrpSer: 1.2 ± 0.26
1.485TrpThr: 1.485 ± 0.312
1.2TrpVal: 1.2 ± 0.199
0.571TrpTrp: 0.571 ± 0.186
0.628TrpTyr: 0.628 ± 0.188
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.113TyrAla: 2.113 ± 0.389
0.114TyrCys: 0.114 ± 0.088
1.714TyrAsp: 1.714 ± 0.256
1.085TyrGlu: 1.085 ± 0.232
0.514TyrPhe: 0.514 ± 0.23
1.599TyrGly: 1.599 ± 0.344
0.4TyrHis: 0.4 ± 0.17
0.628TyrIle: 0.628 ± 0.181
0.514TyrLys: 0.514 ± 0.173
1.485TyrLeu: 1.485 ± 0.345
0.286TyrMet: 0.286 ± 0.13
0.571TyrAsn: 0.571 ± 0.19
0.914TyrPro: 0.914 ± 0.228
0.628TyrGln: 0.628 ± 0.187
2.171TyrArg: 2.171 ± 0.408
1.428TyrSer: 1.428 ± 0.314
1.085TyrThr: 1.085 ± 0.26
2.171TyrVal: 2.171 ± 0.456
0.4TyrTrp: 0.4 ± 0.128
0.8TyrTyr: 0.8 ± 0.201
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 85 proteins (17508 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski