Actinomyces sp. oral taxon 175 str. F0384
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2638 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9PJC1|F9PJC1_9ACTO Transporter major facilitator family protein OS=Actinomyces sp. oral taxon 175 str. F0384 OX=944560 GN=HMPREF9058_2037 PE=4 SV=1
MM1 pKa = 7.03 AQSVHH6 pKa = 4.66 AHH8 pKa = 5.54 LRR10 pKa = 11.84 YY11 pKa = 10.48 SIDD14 pKa = 3.82 HH15 pKa = 6.5 EE16 pKa = 4.19 WLEE19 pKa = 4.7 DD20 pKa = 3.84 GEE22 pKa = 4.41 PACVGITTVAADD34 pKa = 3.59 ALGEE38 pKa = 4.37 VVFVDD43 pKa = 4.61 LPEE46 pKa = 4.76 VGAQVEE52 pKa = 4.19 AGEE55 pKa = 4.32 PCGEE59 pKa = 4.13 LEE61 pKa = 4.31 STKK64 pKa = 10.66 SVSDD68 pKa = 3.46 LVSPVSGTVVAVNDD82 pKa = 3.99 AVVDD86 pKa = 4.12 EE87 pKa = 5.22 PGTINEE93 pKa = 4.54 DD94 pKa = 3.58 PYY96 pKa = 10.65 GAGWLFTVTVSAEE109 pKa = 3.89 GDD111 pKa = 3.57 LLSAQDD117 pKa = 3.83 YY118 pKa = 10.85 ADD120 pKa = 4.14 KK121 pKa = 10.84 FDD123 pKa = 4.24 AVVDD127 pKa = 4.13 GG128 pKa = 4.64
Molecular weight: 13.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.63
IPC_protein 3.605
Toseland 3.401
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.528
Rodwell 3.439
Grimsley 3.312
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 3.91
Thurlkill 3.465
EMBOSS 3.541
Sillero 3.719
Patrickios 0.846
IPC_peptide 3.567
IPC2_peptide 3.694
IPC2.peptide.svr19 3.676
Protein with the highest isoelectric point:
>tr|F9PGZ8|F9PGZ8_9ACTO FeS assembly protein SufD OS=Actinomyces sp. oral taxon 175 str. F0384 OX=944560 GN=sufD PE=3 SV=1
MM1 pKa = 8.02 PEE3 pKa = 3.85 TTFACPDD10 pKa = 3.17 LTTFLGLDD18 pKa = 3.6 ALGLTAVGQLLTSTRR33 pKa = 11.84 AIVEE37 pKa = 3.89 CRR39 pKa = 11.84 MPIGFEE45 pKa = 4.53 DD46 pKa = 4.54 PFCRR50 pKa = 11.84 ACGAQGVSRR59 pKa = 11.84 GTVARR64 pKa = 11.84 RR65 pKa = 11.84 LAHH68 pKa = 6.45 VPVGWRR74 pKa = 11.84 PTQLVVRR81 pKa = 11.84 VRR83 pKa = 11.84 RR84 pKa = 11.84 FACTHH89 pKa = 5.79 CRR91 pKa = 11.84 RR92 pKa = 11.84 VWRR95 pKa = 11.84 QDD97 pKa = 2.98 TSGLAEE103 pKa = 3.78 PRR105 pKa = 11.84 ARR107 pKa = 11.84 LTRR110 pKa = 11.84 SAVEE114 pKa = 3.43 WGLRR118 pKa = 11.84 SLALEE123 pKa = 4.3 CMSVSRR129 pKa = 11.84 VAAALGISWHH139 pKa = 5.43 TANNAILTRR148 pKa = 11.84 AEE150 pKa = 4.13 QILNEE155 pKa = 4.21 APDD158 pKa = 3.4 RR159 pKa = 11.84 FKK161 pKa = 11.13 GVEE164 pKa = 3.7 ALGVDD169 pKa = 3.49 EE170 pKa = 5.09 HH171 pKa = 6.13 VWRR174 pKa = 11.84 HH175 pKa = 4.17 TRR177 pKa = 11.84 RR178 pKa = 11.84 GDD180 pKa = 3.35 RR181 pKa = 11.84 YY182 pKa = 7.78 VTVMIDD188 pKa = 3.35 LTPVRR193 pKa = 11.84 DD194 pKa = 3.45 RR195 pKa = 11.84 SGPARR200 pKa = 11.84 LLDD203 pKa = 3.8 VVPGRR208 pKa = 11.84 SKK210 pKa = 10.88 KK211 pKa = 9.31 VLKK214 pKa = 9.35 TWLSQRR220 pKa = 11.84 DD221 pKa = 3.69 QEE223 pKa = 3.99 WRR225 pKa = 11.84 GRR227 pKa = 11.84 VEE229 pKa = 4.15 VVAMDD234 pKa = 4.21 GFTGFKK240 pKa = 10.09 SAAGEE245 pKa = 4.05 EE246 pKa = 4.49 LPQARR251 pKa = 11.84 AVMDD255 pKa = 4.41 PFHH258 pKa = 7.11 VVALAARR265 pKa = 11.84 KK266 pKa = 9.32 LDD268 pKa = 3.27 QCRR271 pKa = 11.84 RR272 pKa = 11.84 RR273 pKa = 11.84 IQRR276 pKa = 11.84 AITGRR281 pKa = 11.84 RR282 pKa = 11.84 GRR284 pKa = 11.84 AGDD287 pKa = 3.26 RR288 pKa = 11.84 LYY290 pKa = 10.75 RR291 pKa = 11.84 ARR293 pKa = 11.84 RR294 pKa = 11.84 TLLTGAGLLTDD305 pKa = 4.28 AQAEE309 pKa = 4.08 RR310 pKa = 11.84 LEE312 pKa = 4.11 NLFADD317 pKa = 4.11 EE318 pKa = 3.8 RR319 pKa = 11.84 HH320 pKa = 6.13 AAVQAAWGVYY330 pKa = 7.36 QRR332 pKa = 11.84 LIQAYY337 pKa = 7.74 RR338 pKa = 11.84 TEE340 pKa = 4.01 EE341 pKa = 4.11 AGLGKK346 pKa = 10.67 YY347 pKa = 10.32 LMQRR351 pKa = 11.84 LIDD354 pKa = 3.9 SLRR357 pKa = 11.84 QAVPDD362 pKa = 3.89 GLEE365 pKa = 4.33 EE366 pKa = 4.08 IQTLARR372 pKa = 11.84 TLTEE376 pKa = 3.82 RR377 pKa = 11.84 ASDD380 pKa = 2.91 ILAYY384 pKa = 9.92 FDD386 pKa = 5.12 RR387 pKa = 11.84 PRR389 pKa = 11.84 TSNGPTEE396 pKa = 4.92 AINGRR401 pKa = 11.84 LEE403 pKa = 3.89 QLRR406 pKa = 11.84 GIALGFRR413 pKa = 11.84 NLTHH417 pKa = 4.71 YY418 pKa = 8.32 TIRR421 pKa = 11.84 SLIHH425 pKa = 5.41 TGRR428 pKa = 11.84 LKK430 pKa = 11.03 DD431 pKa = 3.65 HH432 pKa = 6.99 LAATTT437 pKa = 3.53
Molecular weight: 48.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.345
IPC2_protein 9.385
IPC_protein 10.452
Toseland 10.482
ProMoST 10.365
Dawson 10.599
Bjellqvist 10.409
Wikipedia 10.877
Rodwell 10.555
Grimsley 10.657
Solomon 10.774
Lehninger 10.73
Nozaki 10.526
DTASelect 10.394
Thurlkill 10.511
EMBOSS 10.906
Sillero 10.555
Patrickios 10.189
IPC_peptide 10.774
IPC2_peptide 9.78
IPC2.peptide.svr19 8.628
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2638
0
2638
910945
35
3150
345.3
36.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.07 ± 0.072
0.776 ± 0.013
5.788 ± 0.035
5.725 ± 0.044
2.485 ± 0.027
8.92 ± 0.049
2.107 ± 0.022
3.963 ± 0.033
2.18 ± 0.036
10.025 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.007 ± 0.021
1.956 ± 0.031
5.706 ± 0.04
3.181 ± 0.026
7.281 ± 0.057
6.546 ± 0.04
6.399 ± 0.038
8.476 ± 0.045
1.468 ± 0.024
1.94 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here