Bacteroidales bacterium Barb7
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2857 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A180FT70|A0A180FT70_9BACT Xylose isomerase OS=Bacteroidales bacterium Barb7 OX=1633203 GN=xylA PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 10.63 NEE4 pKa = 3.98 AQHH7 pKa = 6.82 LIDD10 pKa = 4.57 RR11 pKa = 11.84 FLSARR16 pKa = 11.84 NEE18 pKa = 3.66 GRR20 pKa = 11.84 EE21 pKa = 4.05 AYY23 pKa = 10.27 FDD25 pKa = 3.95 ADD27 pKa = 4.41 EE28 pKa = 5.68 IDD30 pKa = 5.41 DD31 pKa = 4.36 LLEE34 pKa = 4.34 GCEE37 pKa = 4.16 DD38 pKa = 4.24 KK39 pKa = 11.37 EE40 pKa = 5.27 DD41 pKa = 3.82 FDD43 pKa = 5.86 LYY45 pKa = 11.38 EE46 pKa = 4.26 EE47 pKa = 4.32 VLALGLKK54 pKa = 9.13 LHH56 pKa = 7.11 PEE58 pKa = 4.33 NIGLQTKK65 pKa = 9.86 RR66 pKa = 11.84 CWQYY70 pKa = 11.12 VVEE73 pKa = 4.48 EE74 pKa = 4.47 NYY76 pKa = 7.72 TTALEE81 pKa = 4.59 LIEE84 pKa = 4.7 QIGATGDD91 pKa = 3.54 QEE93 pKa = 4.21 IEE95 pKa = 4.05 AVQLEE100 pKa = 5.14 CYY102 pKa = 10.62 LMLDD106 pKa = 3.52 MTDD109 pKa = 3.25 EE110 pKa = 4.56 AEE112 pKa = 4.27 ALSEE116 pKa = 4.18 KK117 pKa = 9.97 MIAEE121 pKa = 4.3 GRR123 pKa = 11.84 EE124 pKa = 4.03 SMDD127 pKa = 3.76 LVFEE131 pKa = 4.61 YY132 pKa = 10.18 IVPLLNDD139 pKa = 3.11 KK140 pKa = 10.7 EE141 pKa = 4.44 MYY143 pKa = 10.43 SLALEE148 pKa = 4.23 YY149 pKa = 11.05 VNRR152 pKa = 11.84 GLEE155 pKa = 4.33 LYY157 pKa = 9.93 PDD159 pKa = 3.69 SLVLKK164 pKa = 10.56 EE165 pKa = 4.13 EE166 pKa = 4.38 LCFLMEE172 pKa = 3.99 VAEE175 pKa = 4.55 DD176 pKa = 3.79 LEE178 pKa = 4.72 GAIDD182 pKa = 4.26 LCNAMIDD189 pKa = 3.83 EE190 pKa = 5.04 DD191 pKa = 4.66 PYY193 pKa = 11.43 SYY195 pKa = 10.78 EE196 pKa = 3.66 YY197 pKa = 10.55 WFTLGRR203 pKa = 11.84 LYY205 pKa = 11.1 SLTEE209 pKa = 4.01 EE210 pKa = 4.18 FEE212 pKa = 4.21 KK213 pKa = 11.1 AIDD216 pKa = 3.9 AFDD219 pKa = 4.04 FAQTCEE225 pKa = 4.32 EE226 pKa = 4.56 PDD228 pKa = 4.07 SEE230 pKa = 5.08 LKK232 pKa = 10.63 LLKK235 pKa = 10.16 AYY237 pKa = 10.56 CFFMSDD243 pKa = 3.44 KK244 pKa = 11.2 YY245 pKa = 11.26 EE246 pKa = 4.19 EE247 pKa = 4.11 ALKK250 pKa = 10.78 LYY252 pKa = 10.34 TEE254 pKa = 4.28 LLKK257 pKa = 11.11 DD258 pKa = 3.57 EE259 pKa = 4.9 EE260 pKa = 4.76 KK261 pKa = 11.02 NGEE264 pKa = 4.23 DD265 pKa = 5.15 GEE267 pKa = 5.07 GDD269 pKa = 3.71 DD270 pKa = 5.24 YY271 pKa = 11.39 ILEE274 pKa = 4.22 RR275 pKa = 11.84 VRR277 pKa = 11.84 PLLAEE282 pKa = 4.56 CYY284 pKa = 8.12 MKK286 pKa = 11.06 LFDD289 pKa = 5.07 YY290 pKa = 10.03 EE291 pKa = 3.82 AAYY294 pKa = 10.48 LILHH298 pKa = 6.42 EE299 pKa = 5.64 LIADD303 pKa = 3.87 DD304 pKa = 5.48 AEE306 pKa = 4.31 AAGYY310 pKa = 7.57 EE311 pKa = 4.3 TDD313 pKa = 4.13 NADD316 pKa = 3.77 TEE318 pKa = 4.86 VNGADD323 pKa = 3.87 TEE325 pKa = 4.76 ADD327 pKa = 3.69 DD328 pKa = 4.69 TDD330 pKa = 3.98 TDD332 pKa = 3.76 STDD335 pKa = 3.33 AARR338 pKa = 11.84 KK339 pKa = 9.87 DD340 pKa = 3.69 YY341 pKa = 11.22 SPYY344 pKa = 9.79 IYY346 pKa = 10.9 YY347 pKa = 7.49 MHH349 pKa = 7.46 CCAQTDD355 pKa = 3.95 RR356 pKa = 11.84 KK357 pKa = 10.22 QDD359 pKa = 3.38 ALNALEE365 pKa = 4.11 TAATVFDD372 pKa = 4.58 GSAHH376 pKa = 5.66 VLSLMAYY383 pKa = 9.82 AYY385 pKa = 9.66 MEE387 pKa = 4.27 SAEE390 pKa = 4.29 NDD392 pKa = 3.58 DD393 pKa = 4.75 NLDD396 pKa = 3.78 VINKK400 pKa = 9.28 LFEE403 pKa = 5.87 LIDD406 pKa = 4.32 DD407 pKa = 5.01 PGDD410 pKa = 4.04 DD411 pKa = 4.94 LEE413 pKa = 5.26 EE414 pKa = 4.38 EE415 pKa = 4.41 ADD417 pKa = 3.71 GDD419 pKa = 4.07 NPSGKK424 pKa = 9.96 DD425 pKa = 3.45 GFSEE429 pKa = 4.73 TIGKK433 pKa = 8.06 MLEE436 pKa = 4.16 YY437 pKa = 10.24 FKK439 pKa = 10.7 KK440 pKa = 10.29 IYY442 pKa = 9.9 AANPDD447 pKa = 3.25 QPYY450 pKa = 10.56 INLYY454 pKa = 10.09 LATAYY459 pKa = 10.62 LINDD463 pKa = 3.48 DD464 pKa = 3.65 MQNFEE469 pKa = 3.9 KK470 pKa = 10.44 HH471 pKa = 5.65 YY472 pKa = 9.78 NLLSAEE478 pKa = 4.34 EE479 pKa = 4.08 LDD481 pKa = 5.01 SYY483 pKa = 10.26 AKK485 pKa = 10.26 KK486 pKa = 10.91 YY487 pKa = 9.94 NAGDD491 pKa = 3.44 SRR493 pKa = 11.84 ALRR496 pKa = 11.84 TIAEE500 pKa = 4.12 KK501 pKa = 10.66 HH502 pKa = 5.34 IGLRR506 pKa = 11.84 DD507 pKa = 3.35 LAEE510 pKa = 4.63 EE511 pKa = 4.16 YY512 pKa = 10.61 LSKK515 pKa = 10.93 PEE517 pKa = 3.94 NSNN520 pKa = 3.08
Molecular weight: 59.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.935
IPC_protein 3.935
Toseland 3.732
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.783
Rodwell 3.757
Grimsley 3.643
Solomon 3.897
Lehninger 3.846
Nozaki 3.999
DTASelect 4.19
Thurlkill 3.757
EMBOSS 3.795
Sillero 4.05
Patrickios 1.074
IPC_peptide 3.897
IPC2_peptide 4.024
IPC2.peptide.svr19 3.928
Protein with the highest isoelectric point:
>tr|A0A180FTK7|A0A180FTK7_9BACT Capsular glucan synthase OS=Bacteroidales bacterium Barb7 OX=1633203 GN=glgA PE=4 SV=1
MM1 pKa = 7.26 LTGDD5 pKa = 4.07 APPYY9 pKa = 10.4 GGVRR13 pKa = 11.84 LCRR16 pKa = 11.84 VAVYY20 pKa = 8.22 GQPVLKK26 pKa = 10.36 EE27 pKa = 3.82 LLLVSEE33 pKa = 4.68 NILRR37 pKa = 11.84 YY38 pKa = 8.42 FTEE41 pKa = 3.77 IDD43 pKa = 3.38 IEE45 pKa = 4.42 VAPLRR50 pKa = 11.84 RR51 pKa = 11.84 RR52 pKa = 11.84 IINEE56 pKa = 3.89 RR57 pKa = 11.84 VKK59 pKa = 10.8 HH60 pKa = 5.67 PKK62 pKa = 10.15 LDD64 pKa = 3.34 ILNVCRR70 pKa = 11.84 FKK72 pKa = 11.24 VALLDD77 pKa = 4.49 FPHH80 pKa = 6.47 HH81 pKa = 6.35 TPPAFLRR88 pKa = 11.84 LGKK91 pKa = 8.93 RR92 pKa = 11.84 TVGIDD97 pKa = 3.27 VVGIEE102 pKa = 4.59 VIGPALIRR110 pKa = 11.84 IIRR113 pKa = 11.84 HH114 pKa = 3.76 IQYY117 pKa = 8.65 PQRR120 pKa = 11.84 GSIAYY125 pKa = 9.43 DD126 pKa = 3.33 LLLVRR131 pKa = 11.84 EE132 pKa = 4.45 KK133 pKa = 10.84 LLHH136 pKa = 6.62 FDD138 pKa = 3.17 GTHH141 pKa = 4.47 IVVRR145 pKa = 11.84 LVVFGDD151 pKa = 3.46 MAGNGVGVFARR162 pKa = 11.84 KK163 pKa = 8.04 YY164 pKa = 7.87 TVNAVPSRR172 pKa = 11.84 QGAVLAVVNVVGKK185 pKa = 10.52 SGFGEE190 pKa = 4.05 AGRR193 pKa = 11.84 GKK195 pKa = 9.94 VVVRR199 pKa = 11.84 RR200 pKa = 11.84 GRR202 pKa = 11.84 DD203 pKa = 3.53 LPCVGVLQAGVDD215 pKa = 3.68 LRR217 pKa = 11.84 AFKK220 pKa = 10.23 IIKK223 pKa = 10.17 VGILNVAACVLALVMTCNQIGKK245 pKa = 9.12 LRR247 pKa = 11.84 LKK249 pKa = 10.63 GYY251 pKa = 9.35 LARR254 pKa = 11.84 TGQRR258 pKa = 11.84 QQRR261 pKa = 11.84 QLVEE265 pKa = 3.77 QVGQLLALRR274 pKa = 11.84 LVGCVQPPQQIINRR288 pKa = 11.84 LIPYY292 pKa = 9.75 GSLHH296 pKa = 5.82 RR297 pKa = 11.84 HH298 pKa = 4.56 RR299 pKa = 11.84 HH300 pKa = 4.61 LQHH303 pKa = 5.3 VHH305 pKa = 6.18 HH306 pKa = 6.86 GAAQRR311 pKa = 11.84 EE312 pKa = 4.21 ILIEE316 pKa = 4.24 LIIHH320 pKa = 5.83 IHH322 pKa = 5.61 AKK324 pKa = 9.9 HH325 pKa = 6.1 RR326 pKa = 11.84 LALHH330 pKa = 5.92 TEE332 pKa = 3.95 HH333 pKa = 6.98 GLVFQRR339 pKa = 11.84 NTDD342 pKa = 3.24 RR343 pKa = 11.84 RR344 pKa = 11.84 IACHH348 pKa = 5.77 NALVEE353 pKa = 4.36 DD354 pKa = 4.74 CNRR357 pKa = 11.84 PHH359 pKa = 7.62 DD360 pKa = 4.57 IIHH363 pKa = 6.74 RR364 pKa = 11.84 IIRR367 pKa = 11.84 ILGKK371 pKa = 10.07 LCPAGCHH378 pKa = 6.24 PYY380 pKa = 10.42 RR381 pKa = 11.84 PASHH385 pKa = 6.68 IRR387 pKa = 11.84 CPQLNLRR394 pKa = 11.84 AA395 pKa = 4.03
Molecular weight: 44.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.589
IPC_protein 10.394
Toseland 10.57
ProMoST 10.277
Dawson 10.687
Bjellqvist 10.423
Wikipedia 10.891
Rodwell 10.847
Grimsley 10.73
Solomon 10.789
Lehninger 10.745
Nozaki 10.584
DTASelect 10.394
Thurlkill 10.584
EMBOSS 10.965
Sillero 10.613
Patrickios 10.526
IPC_peptide 10.789
IPC2_peptide 9.677
IPC2.peptide.svr19 8.517
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2857
0
2857
695753
29
2053
243.5
27.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.637 ± 0.066
1.324 ± 0.025
5.301 ± 0.036
6.184 ± 0.05
4.943 ± 0.039
7.098 ± 0.06
2.067 ± 0.025
6.875 ± 0.057
6.211 ± 0.057
9.225 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.29 ± 0.027
4.807 ± 0.047
3.844 ± 0.036
3.323 ± 0.028
5.131 ± 0.043
6.323 ± 0.047
5.645 ± 0.043
6.774 ± 0.056
0.977 ± 0.019
4.021 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here