Porcine reproductive and respiratory syndrome virus (PRRSV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; unclassified Arteriviridae

Average proteome isoelectric point is 7.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9PX06|Q9PX06_PRRSV Isoform of Q6PR42 2b OS=Porcine reproductive and respiratory syndrome virus OX=28344 GN=ORF2a PE=4 SV=1
MM1 pKa = 7.73GSMQSLFDD9 pKa = 4.68KK10 pKa = 10.64IGQLFVDD17 pKa = 4.8AFTEE21 pKa = 4.33FLVSIVDD28 pKa = 3.88IIIFLAILFGFTIAGWLVVFCIRR51 pKa = 11.84LVCSAILRR59 pKa = 11.84TRR61 pKa = 11.84PAIHH65 pKa = 6.66SEE67 pKa = 3.9QLQKK71 pKa = 10.73ILL73 pKa = 4.36

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6PR44|Q6PR44_PRRSV Non-structural protein 3 OS=Porcine reproductive and respiratory syndrome virus OX=28344 GN=ORF1a PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 9.85WGPCKK7 pKa = 10.65AFLTKK12 pKa = 10.5LANFLWMLSRR22 pKa = 11.84SSWCPLLISLYY33 pKa = 10.16FWPFCLASPSPVGWWSFASDD53 pKa = 3.08WFAPRR58 pKa = 11.84YY59 pKa = 8.69SVRR62 pKa = 11.84ALPFTLSNYY71 pKa = 8.46RR72 pKa = 11.84RR73 pKa = 11.84SYY75 pKa = 10.8EE76 pKa = 3.95AFLSQCQVDD85 pKa = 3.39IPTWGTKK92 pKa = 9.92HH93 pKa = 6.3PLGLLWHH100 pKa = 6.76HH101 pKa = 6.37KK102 pKa = 10.16VSTLIDD108 pKa = 3.53EE109 pKa = 4.49MVSRR113 pKa = 11.84RR114 pKa = 11.84MYY116 pKa = 10.64RR117 pKa = 11.84IMEE120 pKa = 4.22KK121 pKa = 10.19SGQAAWKK128 pKa = 9.72QVVSEE133 pKa = 4.27ATLSRR138 pKa = 11.84ISSLDD143 pKa = 3.43VVAHH147 pKa = 6.05FQHH150 pKa = 6.68LAAIEE155 pKa = 4.4AEE157 pKa = 4.33TCKK160 pKa = 10.76YY161 pKa = 9.79LASRR165 pKa = 11.84LPMLHH170 pKa = 6.56NLRR173 pKa = 11.84MTGSNVTIVYY183 pKa = 10.47NSTLNQVFAIFPTPGSRR200 pKa = 11.84PKK202 pKa = 10.85LHH204 pKa = 7.62DD205 pKa = 3.61FQQWLIAVHH214 pKa = 6.43SSIFSSVAASCTLFVVLWLRR234 pKa = 11.84VPILRR239 pKa = 11.84TVFGFRR245 pKa = 11.84WLGAIFLSNSQQ256 pKa = 3.17

Molecular weight: 29.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

1

9

5218

73

2503

579.8

63.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.432 ± 0.425

3.085 ± 0.218

4.427 ± 0.548

4.293 ± 0.458

4.561 ± 0.581

7.11 ± 0.701

2.587 ± 0.318

4.044 ± 0.472

4.484 ± 0.352

10.33 ± 0.385

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.955 ± 0.169

3.085 ± 0.146

6.784 ± 0.889

3.22 ± 0.321

5.424 ± 0.152

7.11 ± 0.715

5.96 ± 0.351

8.49 ± 0.34

1.84 ± 0.298

2.779 ± 0.586
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski