Seal anellovirus TFFN/USA/2006

Taxonomy: Viruses; Anelloviridae; unclassified Anelloviridae

Average proteome isoelectric point is 6.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F1CHU6|F1CHU6_9VIRU Orf3 OS=Seal anellovirus TFFN/USA/2006 OX=991022 PE=4 SV=1
MM1 pKa = 6.51TTLMTQKK8 pKa = 10.5KK9 pKa = 9.63PMMPEE14 pKa = 3.67QPGLEE19 pKa = 4.52GINIKK24 pKa = 10.11CLHH27 pKa = 6.86ANPILQKK34 pKa = 9.17QAKK37 pKa = 6.81TVIVLTGALSYY48 pKa = 11.45LNAFPCGILGADD60 pKa = 3.66PYY62 pKa = 11.31GLNINSFLSSLEE74 pKa = 3.99TLSTEE79 pKa = 4.07EE80 pKa = 4.47QSAKK84 pKa = 9.34TPKK87 pKa = 9.86TSSLKK92 pKa = 10.25LRR94 pKa = 11.84RR95 pKa = 11.84ANTLKK100 pKa = 10.52EE101 pKa = 4.11KK102 pKa = 10.86YY103 pKa = 9.74RR104 pKa = 11.84LAPLQGGRR112 pKa = 11.84SMRR115 pKa = 11.84RR116 pKa = 11.84TSSEE120 pKa = 4.35GILTPQGSSRR130 pKa = 11.84RR131 pKa = 11.84EE132 pKa = 4.01LIEE135 pKa = 4.04EE136 pKa = 4.2LLDD139 pKa = 3.79LVEE142 pKa = 6.18KK143 pKa = 10.78SSQPCWCQKK152 pKa = 10.43EE153 pKa = 4.31SDD155 pKa = 4.02SGEE158 pKa = 4.22EE159 pKa = 3.85TSDD162 pKa = 4.4ASDD165 pKa = 3.57SDD167 pKa = 3.41ISYY170 pKa = 10.8LDD172 pKa = 3.21SWSDD176 pKa = 3.42EE177 pKa = 4.15EE178 pKa = 4.65NPGGEE183 pKa = 4.45GAPLVPPQGGAPPRR197 pKa = 11.84YY198 pKa = 8.66PSQFLFF204 pKa = 4.03

Molecular weight: 22.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F1CHU6|F1CHU6_9VIRU Orf3 OS=Seal anellovirus TFFN/USA/2006 OX=991022 PE=4 SV=1
MM1 pKa = 7.47ARR3 pKa = 11.84RR4 pKa = 11.84FRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84FQRR11 pKa = 11.84WRR13 pKa = 11.84RR14 pKa = 11.84GRR16 pKa = 11.84YY17 pKa = 8.06HH18 pKa = 6.89PRR20 pKa = 11.84RR21 pKa = 11.84GYY23 pKa = 9.72RR24 pKa = 11.84RR25 pKa = 11.84WRR27 pKa = 11.84PFHH30 pKa = 6.58HH31 pKa = 5.74WRR33 pKa = 11.84HH34 pKa = 3.62WRR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84GRR40 pKa = 11.84YY41 pKa = 8.21FRR43 pKa = 11.84HH44 pKa = 4.86RR45 pKa = 11.84TAAVRR50 pKa = 11.84YY51 pKa = 8.65RR52 pKa = 11.84PSRR55 pKa = 11.84RR56 pKa = 11.84HH57 pKa = 5.23KK58 pKa = 10.78VVVCRR63 pKa = 11.84GWEE66 pKa = 3.74PLGNVCKK73 pKa = 9.48EE74 pKa = 4.09TLASQEE80 pKa = 3.79ATPYY84 pKa = 10.88KK85 pKa = 10.43DD86 pKa = 3.68IEE88 pKa = 4.49ADD90 pKa = 3.59YY91 pKa = 11.32DD92 pKa = 3.94PTTSTLDD99 pKa = 3.2QFKK102 pKa = 10.86GSWQGTWGHH111 pKa = 6.63HH112 pKa = 5.01YY113 pKa = 8.0FTLEE117 pKa = 3.81NLVKK121 pKa = 10.67RR122 pKa = 11.84NRR124 pKa = 11.84QLMNTFTSDD133 pKa = 2.47WRR135 pKa = 11.84GWDD138 pKa = 3.39YY139 pKa = 11.44YY140 pKa = 11.31QFLGGVFYY148 pKa = 10.26MPKK151 pKa = 9.68ISGMDD156 pKa = 3.07YY157 pKa = 10.73FIYY160 pKa = 10.54LDD162 pKa = 4.49YY163 pKa = 11.33DD164 pKa = 3.84LQDD167 pKa = 4.03LKK169 pKa = 11.6AKK171 pKa = 10.51GDD173 pKa = 3.51EE174 pKa = 4.1TDD176 pKa = 3.92LYY178 pKa = 11.53KK179 pKa = 10.87HH180 pKa = 5.68MKK182 pKa = 9.33SWIHH186 pKa = 5.89PGIYY190 pKa = 8.58MNRR193 pKa = 11.84YY194 pKa = 6.29GAKK197 pKa = 9.56MITSPHH203 pKa = 6.1RR204 pKa = 11.84TTAPRR209 pKa = 11.84LFRR212 pKa = 11.84KK213 pKa = 9.74VRR215 pKa = 11.84IKK217 pKa = 10.71PPASWEE223 pKa = 3.75GLYY226 pKa = 10.17PINTGFKK233 pKa = 10.82YY234 pKa = 10.31IFCHH238 pKa = 5.69WAWTVFDD245 pKa = 3.62WDD247 pKa = 3.8MPFFDD252 pKa = 4.72SYY254 pKa = 11.8CYY256 pKa = 10.55LKK258 pKa = 10.79SCATKK263 pKa = 10.02PPKK266 pKa = 9.96PMCEE270 pKa = 3.82QCCNEE275 pKa = 3.98RR276 pKa = 11.84PWWSGLKK283 pKa = 10.14DD284 pKa = 3.43SHH286 pKa = 7.01NMCKK290 pKa = 10.25PNFDD294 pKa = 5.7DD295 pKa = 5.06NFNDD299 pKa = 3.85SKK301 pKa = 10.91EE302 pKa = 4.42AYY304 pKa = 8.63DD305 pKa = 5.73ARR307 pKa = 11.84ATWVRR312 pKa = 11.84RR313 pKa = 11.84DD314 pKa = 3.5QYY316 pKa = 11.74QMSSCEE322 pKa = 4.19SNTPEE327 pKa = 4.17TGKK330 pKa = 10.48DD331 pKa = 3.45CNCFNWGPFLPQCFPLWNSWGRR353 pKa = 11.84SVWFKK358 pKa = 11.27YY359 pKa = 10.7KK360 pKa = 10.82LFFKK364 pKa = 10.92FSGDD368 pKa = 3.06SVYY371 pKa = 10.82RR372 pKa = 11.84RR373 pKa = 11.84TVSKK377 pKa = 10.56DD378 pKa = 3.24SKK380 pKa = 11.24DD381 pKa = 4.44LIPEE385 pKa = 4.25APKK388 pKa = 10.74GKK390 pKa = 9.91YY391 pKa = 8.93SQGEE395 pKa = 4.31IPSSSSPRR403 pKa = 11.84RR404 pKa = 11.84PLDD407 pKa = 3.23EE408 pKa = 6.4ADD410 pKa = 3.55ILRR413 pKa = 11.84GDD415 pKa = 4.89LDD417 pKa = 3.68PSGILTEE424 pKa = 3.9RR425 pKa = 11.84AYY427 pKa = 10.8RR428 pKa = 11.84GITRR432 pKa = 11.84PGGEE436 pKa = 4.49EE437 pKa = 4.04QPTMLVPKK445 pKa = 9.89RR446 pKa = 11.84VRR448 pKa = 11.84FRR450 pKa = 11.84RR451 pKa = 11.84GDD453 pKa = 3.08IRR455 pKa = 11.84RR456 pKa = 11.84KK457 pKa = 9.6RR458 pKa = 11.84LRR460 pKa = 11.84HH461 pKa = 4.93LLSRR465 pKa = 11.84LMEE468 pKa = 4.19RR469 pKa = 4.35

Molecular weight: 56.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

784

111

469

261.3

30.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.592 ± 0.746

2.423 ± 0.329

5.612 ± 0.749

5.23 ± 1.248

4.592 ± 1.045

7.653 ± 1.594

2.296 ± 0.727

4.209 ± 1.263

5.995 ± 0.49

8.291 ± 2.137

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.679 ± 0.094

3.444 ± 0.331

6.633 ± 1.152

3.827 ± 0.98

9.056 ± 2.874

7.143 ± 2.005

5.102 ± 0.729

3.444 ± 1.092

3.316 ± 1.088

4.464 ± 0.999
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski