Trichomonas vaginalis virus 2
Average proteome isoelectric point is 7.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9JE95|Q9JE95_9VIRU Capsid protein OS=Trichomonas vaginalis virus 2 OX=674954 GN=cap PE=4 SV=1
MM1 pKa = 7.93 ASTLISSDD9 pKa = 3.08 NSATLGKK16 pKa = 10.35 DD17 pKa = 3.02 SEE19 pKa = 4.95 VINNTNTSPPDD30 pKa = 3.64 NPSSDD35 pKa = 3.19 HH36 pKa = 6.59 SNPRR40 pKa = 11.84 LTKK43 pKa = 10.68 VLDD46 pKa = 3.76 EE47 pKa = 4.31 MSKK50 pKa = 10.5 KK51 pKa = 10.01 PCVNINEE58 pKa = 3.97 IRR60 pKa = 11.84 KK61 pKa = 8.57 MIRR64 pKa = 11.84 NFQPQFIQPRR74 pKa = 11.84 NGNRR78 pKa = 11.84 PNAQPRR84 pKa = 11.84 TVDD87 pKa = 3.01 SFEE90 pKa = 3.93 WVVRR94 pKa = 11.84 IQSTVEE100 pKa = 3.96 TQLLGATNTVPQQTLNLDD118 pKa = 3.11 ISFTDD123 pKa = 3.96 DD124 pKa = 3.09 STTITPASIPGSISMLDD141 pKa = 3.55 NSRR144 pKa = 11.84 HH145 pKa = 5.08 IPAIQSMIQNFKK157 pKa = 10.91 ARR159 pKa = 11.84 YY160 pKa = 8.88 LGSLQDD166 pKa = 3.7 TAQLQSPQYY175 pKa = 8.61 PQLLAYY181 pKa = 10.86 LFGQLIAIKK190 pKa = 10.48 DD191 pKa = 3.73 RR192 pKa = 11.84 LDD194 pKa = 3.27 LFRR197 pKa = 11.84 PSNPLSLADD206 pKa = 3.75 ALFGFTLAQNARR218 pKa = 11.84 PRR220 pKa = 11.84 YY221 pKa = 9.04 DD222 pKa = 3.31 DD223 pKa = 3.73 HH224 pKa = 8.01 RR225 pKa = 11.84 HH226 pKa = 5.62 AKK228 pKa = 9.39 ACQGPLVIPAATNSDD243 pKa = 4.07 CGPCGFVQINANQGLTLPLGACLFVNPEE271 pKa = 4.19 TVNDD275 pKa = 3.5 QSFQDD280 pKa = 3.96 FLWLIFATHH289 pKa = 6.09 HH290 pKa = 6.33 RR291 pKa = 11.84 MPNQMQNNWPFSLNIVSTCAAPGRR315 pKa = 11.84 QAPHH319 pKa = 6.56 AGEE322 pKa = 4.11 LTDD325 pKa = 3.43 EE326 pKa = 4.34 RR327 pKa = 11.84 VRR329 pKa = 11.84 LALDD333 pKa = 3.26 TGHH336 pKa = 7.49 RR337 pKa = 11.84 ILLSMFNDD345 pKa = 3.74 DD346 pKa = 4.95 EE347 pKa = 4.42 EE348 pKa = 4.43 TLRR351 pKa = 11.84 YY352 pKa = 8.24 YY353 pKa = 9.44 QRR355 pKa = 11.84 KK356 pKa = 9.4 GIEE359 pKa = 4.05 TMFRR363 pKa = 11.84 PCCFYY368 pKa = 11.09 TEE370 pKa = 4.28 GGLLRR375 pKa = 11.84 KK376 pKa = 8.05 ATRR379 pKa = 11.84 YY380 pKa = 10.52 VSMVPLNGLYY390 pKa = 10.11 YY391 pKa = 10.4 YY392 pKa = 10.82 NGATSYY398 pKa = 10.68 VVSPIHH404 pKa = 6.46 TDD406 pKa = 2.87 AHH408 pKa = 6.09 PGITAAIEE416 pKa = 4.22 SFVDD420 pKa = 3.44 IMVLQAVFSFSGPKK434 pKa = 9.47 VVAAKK439 pKa = 10.65 VNASQIDD446 pKa = 3.45 AAMVFGPAVAEE457 pKa = 4.16 GDD459 pKa = 3.77 GFVYY463 pKa = 10.54 DD464 pKa = 4.78 PLRR467 pKa = 11.84 PAPPLSAFYY476 pKa = 10.67 TEE478 pKa = 5.78 FIHH481 pKa = 7.35 RR482 pKa = 11.84 PAEE485 pKa = 3.77 QRR487 pKa = 11.84 IFQMAMSQIYY497 pKa = 10.02 GSHH500 pKa = 6.77 APLIIANVINSIHH513 pKa = 5.59 NCKK516 pKa = 9.21 TKK518 pKa = 10.35 IVNNKK523 pKa = 8.71 LRR525 pKa = 11.84 ATFVRR530 pKa = 11.84 RR531 pKa = 11.84 PPGAPHH537 pKa = 7.04 LKK539 pKa = 10.38 ADD541 pKa = 3.58 TAIINRR547 pKa = 11.84 FHH549 pKa = 7.29 DD550 pKa = 4.02 PEE552 pKa = 4.14 LAYY555 pKa = 10.6 ALGILADD562 pKa = 5.49 GIAPLDD568 pKa = 3.94 GSHH571 pKa = 6.94 EE572 pKa = 4.26 YY573 pKa = 10.78 NVLDD577 pKa = 3.95 EE578 pKa = 5.65 LDD580 pKa = 3.58 YY581 pKa = 11.49 LFNGGDD587 pKa = 3.14 IRR589 pKa = 11.84 NCFGLNALNTRR600 pKa = 11.84 GLGQIVHH607 pKa = 6.63 IRR609 pKa = 11.84 PKK611 pKa = 10.19 RR612 pKa = 11.84 EE613 pKa = 3.37 PGKK616 pKa = 9.46 RR617 pKa = 11.84 PRR619 pKa = 11.84 RR620 pKa = 11.84 GFYY623 pKa = 7.56 TTLDD627 pKa = 3.99 GQVHH631 pKa = 7.0 PVTQDD636 pKa = 2.83 APLDD640 pKa = 4.48 EE641 pKa = 5.1 IYY643 pKa = 10.24 HH644 pKa = 5.68 WRR646 pKa = 11.84 DD647 pKa = 2.85 HH648 pKa = 6.76 GNLTRR653 pKa = 11.84 PYY655 pKa = 9.27 SCHH658 pKa = 7.05 ILDD661 pKa = 3.83 SQGLEE666 pKa = 3.99 FADD669 pKa = 3.66 VSNGRR674 pKa = 11.84 SRR676 pKa = 11.84 GKK678 pKa = 9.94 ILVVVNSPLKK688 pKa = 9.97 TCAAYY693 pKa = 9.98 QGPSFAPKK701 pKa = 9.62 PGSAMWNEE709 pKa = 4.0
Molecular weight: 78.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.509
IPC2_protein 6.517
IPC_protein 6.605
Toseland 6.737
ProMoST 6.956
Dawson 7.059
Bjellqvist 7.059
Wikipedia 7.059
Rodwell 7.059
Grimsley 6.854
Solomon 7.073
Lehninger 7.073
Nozaki 7.38
DTASelect 7.424
Thurlkill 7.468
EMBOSS 7.454
Sillero 7.483
Patrickios 4.139
IPC_peptide 7.088
IPC2_peptide 7.176
IPC2.peptide.svr19 6.953
Protein with the highest isoelectric point:
>tr|Q9JE95|Q9JE95_9VIRU Capsid protein OS=Trichomonas vaginalis virus 2 OX=674954 GN=cap PE=4 SV=1
RR1 pKa = 6.96 FQRR4 pKa = 11.84 TVTRR8 pKa = 11.84 KK9 pKa = 10.17 DD10 pKa = 2.97 PCGRR14 pKa = 11.84 QLTPQDD20 pKa = 3.94 MRR22 pKa = 11.84 CLPGPQLRR30 pKa = 11.84 AKK32 pKa = 10.21 AGQRR36 pKa = 11.84 HH37 pKa = 4.89 VEE39 pKa = 4.54 RR40 pKa = 11.84 INQCGGSVIYY50 pKa = 9.88 PRR52 pKa = 11.84 LSTARR57 pKa = 11.84 ACSLSAIDD65 pKa = 5.98 KK66 pKa = 7.35 MTLALAHH73 pKa = 5.55 QLCYY77 pKa = 10.25 LYY79 pKa = 10.69 KK80 pKa = 10.67 SSDD83 pKa = 3.19 LHH85 pKa = 6.17 RR86 pKa = 11.84 QLDD89 pKa = 3.77 TMIPQSYY96 pKa = 8.0 LTFLEE101 pKa = 3.91 WLLRR105 pKa = 11.84 IDD107 pKa = 3.48 PHH109 pKa = 6.93 NEE111 pKa = 3.42 KK112 pKa = 10.74 SSIRR116 pKa = 11.84 HH117 pKa = 5.58 FPSQDD122 pKa = 2.63 NHH124 pKa = 5.29 EE125 pKa = 5.0 VITHH129 pKa = 5.65 SLRR132 pKa = 11.84 NLTKK136 pKa = 9.6 EE137 pKa = 3.83 QEE139 pKa = 3.93 ITLFPIKK146 pKa = 10.52 DD147 pKa = 2.98 IVQANRR153 pKa = 11.84 RR154 pKa = 11.84 VNAYY158 pKa = 9.82 ARR160 pKa = 11.84 NLLDD164 pKa = 5.15 ASPLPDD170 pKa = 3.77 FALQQMLLPNTANDD184 pKa = 3.8 VVCAILLLGEE194 pKa = 4.42 VLWMLRR200 pKa = 11.84 CPISIIVNISRR211 pKa = 11.84 AICRR215 pKa = 11.84 NDD217 pKa = 3.35 SFLKK221 pKa = 10.74 DD222 pKa = 3.43 LSDD225 pKa = 3.84 FNKK228 pKa = 9.93 MLGLTKK234 pKa = 10.12 IPIANCLTEE243 pKa = 5.51 LNTLQGRR250 pKa = 11.84 GVTSSDD256 pKa = 2.82 AKK258 pKa = 10.61 RR259 pKa = 11.84 DD260 pKa = 3.64 LTHH263 pKa = 7.56 RR264 pKa = 11.84 IADD267 pKa = 4.02 VNPHH271 pKa = 5.22 EE272 pKa = 4.8 AKK274 pKa = 10.25 ISRR277 pKa = 11.84 EE278 pKa = 3.85 NLRR281 pKa = 11.84 EE282 pKa = 4.51 AINQIYY288 pKa = 9.9 KK289 pKa = 10.78 EE290 pKa = 4.17 EE291 pKa = 4.08 ITRR294 pKa = 11.84 KK295 pKa = 9.16 EE296 pKa = 4.0 VPDD299 pKa = 3.52 TFKK302 pKa = 10.9 QHH304 pKa = 6.13 VFTSPLWVKK313 pKa = 10.53 KK314 pKa = 9.87 GAHH317 pKa = 5.49 HH318 pKa = 6.72 HH319 pKa = 6.49 PLFGSYY325 pKa = 10.84 DD326 pKa = 3.38 NRR328 pKa = 11.84 LEE330 pKa = 3.97 FVEE333 pKa = 5.31 NVDD336 pKa = 3.68 LDD338 pKa = 4.13 RR339 pKa = 11.84 VLKK342 pKa = 9.91 SHH344 pKa = 6.76 PAVYY348 pKa = 8.77 ITQAPKK354 pKa = 10.64 LEE356 pKa = 4.34 HH357 pKa = 6.1 GKK359 pKa = 8.01 TRR361 pKa = 11.84 FIYY364 pKa = 10.5 NCDD367 pKa = 3.39 TVSYY371 pKa = 10.35 IYY373 pKa = 10.47 FDD375 pKa = 4.16 YY376 pKa = 10.49 ILNYY380 pKa = 9.58 IEE382 pKa = 5.32 SVWSNKK388 pKa = 9.02 HH389 pKa = 5.07 VLLNPDD395 pKa = 3.29 YY396 pKa = 9.06 MNPVIFSTLNYY407 pKa = 10.36 DD408 pKa = 4.33 EE409 pKa = 5.48 YY410 pKa = 11.96 CMLDD414 pKa = 3.32 YY415 pKa = 11.32 TDD417 pKa = 6.17 FNSQHH422 pKa = 6.72 SIEE425 pKa = 4.36 SMKK428 pKa = 10.7 QVFLCLFPFLPRR440 pKa = 11.84 SMHH443 pKa = 7.42 SILQWCVTSFDD454 pKa = 3.3 NMYY457 pKa = 10.56 INKK460 pKa = 7.62 THH462 pKa = 6.8 WNSTLPSGHH471 pKa = 7.23 RR472 pKa = 11.84 ATTFINSVLNRR483 pKa = 11.84 AYY485 pKa = 10.82 LLPFLQVANAFHH497 pKa = 6.54 TGDD500 pKa = 4.89 DD501 pKa = 3.56 VLLCGKK507 pKa = 10.2 ADD509 pKa = 3.63 YY510 pKa = 7.84 ATLINTVPYY519 pKa = 9.77 EE520 pKa = 3.98 LNKK523 pKa = 9.5 TKK525 pKa = 10.72 QSFGPSAEE533 pKa = 3.75 FLRR536 pKa = 11.84 LHH538 pKa = 6.04 KK539 pKa = 10.65 HH540 pKa = 5.41 NDD542 pKa = 3.25 QVSGYY547 pKa = 7.28 PARR550 pKa = 11.84 AISSLVSGNWLSFANPLWQPSLLSIMQQLYY580 pKa = 9.42 TISARR585 pKa = 11.84 SGLLPYY591 pKa = 10.26 IPVTMKK597 pKa = 11.03 LEE599 pKa = 3.95 VQRR602 pKa = 11.84 RR603 pKa = 11.84 YY604 pKa = 10.8 DD605 pKa = 3.39 LRR607 pKa = 11.84 SRR609 pKa = 11.84 ITNGLFSGDD618 pKa = 3.96 IVPSGCPCYY627 pKa = 10.27 KK628 pKa = 10.65 SNAALLSAVVPDD640 pKa = 4.35 TVVKK644 pKa = 10.68 APPTFYY650 pKa = 11.06 DD651 pKa = 4.84 LRR653 pKa = 11.84 TLDD656 pKa = 3.59 ILKK659 pKa = 8.41 QTSPWINSASRR670 pKa = 11.84 YY671 pKa = 8.72 MDD673 pKa = 3.86 LLEE676 pKa = 4.28 RR677 pKa = 11.84 RR678 pKa = 11.84 HH679 pKa = 5.83 MEE681 pKa = 3.87 SDD683 pKa = 3.3 NKK685 pKa = 10.36 NVIYY689 pKa = 10.28 SIQYY693 pKa = 9.77 LPSKK697 pKa = 8.38 MLPMIDD703 pKa = 4.33 VDD705 pKa = 4.23 PADD708 pKa = 3.84 ATPLRR713 pKa = 11.84 KK714 pKa = 9.04 RR715 pKa = 11.84 YY716 pKa = 9.37 HH717 pKa = 5.94 PRR719 pKa = 11.84 SHH721 pKa = 7.02 IAHH724 pKa = 7.37 PLPRR728 pKa = 11.84 DD729 pKa = 3.3 AHH731 pKa = 5.86 LKK733 pKa = 8.18 EE734 pKa = 4.81 LRR736 pKa = 11.84 FATCRR741 pKa = 11.84 VGPATAIRR749 pKa = 11.84 LGSLWPANRR758 pKa = 11.84 INLIKK763 pKa = 10.1 PVYY766 pKa = 9.27 VV767 pKa = 3.37
Molecular weight: 87.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.352
IPC2_protein 8.331
IPC_protein 8.17
Toseland 8.273
ProMoST 8.756
Dawson 8.96
Bjellqvist 9.092
Wikipedia 9.033
Rodwell 9.033
Grimsley 8.492
Solomon 9.019
Lehninger 8.99
Nozaki 9.209
DTASelect 8.902
Thurlkill 8.96
EMBOSS 9.107
Sillero 9.209
Patrickios 4.304
IPC_peptide 9.004
IPC2_peptide 8.039
IPC2.peptide.svr19 7.93
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1476
709
767
738.0
83.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.249 ± 0.885
1.965 ± 0.199
5.691 ± 0.067
3.32 ± 0.055
4.133 ± 0.381
4.607 ± 1.166
3.32 ± 0.261
6.369 ± 0.016
3.862 ± 0.656
10.434 ± 1.129
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.168 ± 0.038
6.098 ± 0.285
6.978 ± 0.465
4.472 ± 0.442
6.301 ± 0.275
7.182 ± 0.403
5.691 ± 0.067
5.488 ± 0.093
1.016 ± 0.227
3.659 ± 0.405
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here