Amino acid dipepetide frequency for Flavobacteriaceae bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.563AlaAla: 3.563 ± 0.088	0.496AlaCys: 0.496 ± 0.026	2.866AlaAsp: 2.866 ± 0.086	3.4AlaGlu: 3.4 ± 0.076	3.253AlaPhe: 3.253 ± 0.059	3.589AlaGly: 3.589 ± 0.075	1.032AlaHis: 1.032 ± 0.033	5.846AlaIle: 5.846 ± 0.083	4.392AlaLys: 4.392 ± 0.084	6.02AlaLeu: 6.02 ± 0.086	1.287AlaMet: 1.287 ± 0.037	3.528AlaAsn: 3.528 ± 0.076	1.683AlaPro: 1.683 ± 0.049	2.181AlaGln: 2.181 ± 0.078	1.856AlaArg: 1.856 ± 0.05	4.053AlaSer: 4.053 ± 0.071	3.343AlaThr: 3.343 ± 0.109	3.376AlaVal: 3.376 ± 0.066	0.528AlaTrp: 0.528 ± 0.021	2.405AlaTyr: 2.405 ± 0.055	0.0AlaXaa: 0.0 ± 0.0
Cys	0.379CysAla: 0.379 ± 0.022	0.067CysCys: 0.067 ± 0.008	0.423CysAsp: 0.423 ± 0.043	0.394CysGlu: 0.394 ± 0.021	0.443CysPhe: 0.443 ± 0.041	0.537CysGly: 0.537 ± 0.032	0.162CysHis: 0.162 ± 0.014	0.656CysIle: 0.656 ± 0.028	0.464CysLys: 0.464 ± 0.022	0.581CysLeu: 0.581 ± 0.027	0.138CysMet: 0.138 ± 0.012	0.405CysAsn: 0.405 ± 0.019	0.279CysPro: 0.279 ± 0.022	0.18CysGln: 0.18 ± 0.012	0.177CysArg: 0.177 ± 0.014	0.48CysSer: 0.48 ± 0.026	0.428CysThr: 0.428 ± 0.063	0.389CysVal: 0.389 ± 0.02	0.059CysTrp: 0.059 ± 0.008	0.261CysTyr: 0.261 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.502AspAla: 3.502 ± 0.076	0.398AspCys: 0.398 ± 0.044	3.109AspAsp: 3.109 ± 0.073	3.528AspGlu: 3.528 ± 0.069	3.498AspPhe: 3.498 ± 0.066	3.672AspGly: 3.672 ± 0.164	0.843AspHis: 0.843 ± 0.029	4.865AspIle: 4.865 ± 0.073	3.955AspLys: 3.955 ± 0.077	5.164AspLeu: 5.164 ± 0.068	0.983AspMet: 0.983 ± 0.034	3.565AspAsn: 3.565 ± 0.064	1.591AspPro: 1.591 ± 0.049	1.359AspGln: 1.359 ± 0.033	1.811AspArg: 1.811 ± 0.042	3.218AspSer: 3.218 ± 0.072	2.93AspThr: 2.93 ± 0.07	3.637AspVal: 3.637 ± 0.078	0.668AspTrp: 0.668 ± 0.022	2.749AspTyr: 2.749 ± 0.059	0.0AspXaa: 0.0 ± 0.0
Glu	4.106GluAla: 4.106 ± 0.077	0.32GluCys: 0.32 ± 0.019	3.572GluAsp: 3.572 ± 0.062	4.499GluGlu: 4.499 ± 0.093	3.133GluPhe: 3.133 ± 0.06	3.654GluGly: 3.654 ± 0.067	1.162GluHis: 1.162 ± 0.037	5.754GluIle: 5.754 ± 0.097	5.167GluLys: 5.167 ± 0.101	6.311GluLeu: 6.311 ± 0.083	1.315GluMet: 1.315 ± 0.037	4.736GluAsn: 4.736 ± 0.084	1.526GluPro: 1.526 ± 0.063	2.292GluGln: 2.292 ± 0.053	2.576GluArg: 2.576 ± 0.063	3.383GluSer: 3.383 ± 0.062	3.784GluThr: 3.784 ± 0.064	3.99GluVal: 3.99 ± 0.064	0.551GluTrp: 0.551 ± 0.021	2.396GluTyr: 2.396 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	2.686PheAla: 2.686 ± 0.054	0.409PheCys: 0.409 ± 0.019	3.216PheAsp: 3.216 ± 0.059	3.396PheGlu: 3.396 ± 0.058	2.886PhePhe: 2.886 ± 0.089	3.692PheGly: 3.692 ± 0.105	0.773PheHis: 0.773 ± 0.031	4.694PheIle: 4.694 ± 0.083	4.305PheLys: 4.305 ± 0.074	4.913PheLeu: 4.913 ± 0.103	0.995PheMet: 0.995 ± 0.033	3.933PheAsn: 3.933 ± 0.073	1.676PhePro: 1.676 ± 0.04	1.459PheGln: 1.459 ± 0.041	1.598PheArg: 1.598 ± 0.039	4.133PheSer: 4.133 ± 0.071	3.08PheThr: 3.08 ± 0.065	2.871PheVal: 2.871 ± 0.059	0.584PheTrp: 0.584 ± 0.028	2.187PheTyr: 2.187 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	3.939GlyAla: 3.939 ± 0.091	0.493GlyCys: 0.493 ± 0.027	3.384GlyAsp: 3.384 ± 0.112	3.609GlyGlu: 3.609 ± 0.068	3.733GlyPhe: 3.733 ± 0.066	4.568GlyGly: 4.568 ± 0.104	1.088GlyHis: 1.088 ± 0.033	5.429GlyIle: 5.429 ± 0.077	4.663GlyLys: 4.663 ± 0.085	5.616GlyLeu: 5.616 ± 0.081	1.317GlyMet: 1.317 ± 0.039	3.802GlyAsn: 3.802 ± 0.109	1.326GlyPro: 1.326 ± 0.05	1.742GlyGln: 1.742 ± 0.045	2.126GlyArg: 2.126 ± 0.052	3.81GlySer: 3.81 ± 0.077	3.722GlyThr: 3.722 ± 0.099	4.161GlyVal: 4.161 ± 0.088	0.694GlyTrp: 0.694 ± 0.026	2.609GlyTyr: 2.609 ± 0.058	0.0GlyXaa: 0.0 ± 0.0
His	0.779HisAla: 0.779 ± 0.028	0.177HisCys: 0.177 ± 0.014	0.723HisAsp: 0.723 ± 0.029	0.805HisGlu: 0.805 ± 0.03	1.13HisPhe: 1.13 ± 0.032	0.948HisGly: 0.948 ± 0.03	0.473HisHis: 0.473 ± 0.023	1.651HisIle: 1.651 ± 0.041	1.282HisLys: 1.282 ± 0.035	1.768HisLeu: 1.768 ± 0.048	0.313HisMet: 0.313 ± 0.017	1.082HisAsn: 1.082 ± 0.034	0.854HisPro: 0.854 ± 0.032	0.734HisGln: 0.734 ± 0.028	0.663HisArg: 0.663 ± 0.027	1.045HisSer: 1.045 ± 0.031	0.985HisThr: 0.985 ± 0.033	0.732HisVal: 0.732 ± 0.03	0.196HisTrp: 0.196 ± 0.015	0.812HisTyr: 0.812 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.857IleAla: 5.857 ± 0.077	0.595IleCys: 0.595 ± 0.025	5.44IleAsp: 5.44 ± 0.077	6.102IleGlu: 6.102 ± 0.098	4.053IlePhe: 4.053 ± 0.085	5.441IleGly: 5.441 ± 0.09	1.422IleHis: 1.422 ± 0.039	7.713IleIle: 7.713 ± 0.123	6.908IleLys: 6.908 ± 0.113	7.8IleLeu: 7.8 ± 0.108	1.41IleMet: 1.41 ± 0.041	6.033IleAsn: 6.033 ± 0.09	3.454IlePro: 3.454 ± 0.064	2.698IleGln: 2.698 ± 0.063	2.622IleArg: 2.622 ± 0.054	6.616IleSer: 6.616 ± 0.084	5.675IleThr: 5.675 ± 0.159	5.164IleVal: 5.164 ± 0.08	0.795IleTrp: 0.795 ± 0.043	3.12IleTyr: 3.12 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	4.862LysAla: 4.862 ± 0.092	0.334LysCys: 0.334 ± 0.019	4.417LysAsp: 4.417 ± 0.083	6.159LysGlu: 6.159 ± 0.122	2.952LysPhe: 2.952 ± 0.066	4.474LysGly: 4.474 ± 0.086	1.491LysHis: 1.491 ± 0.039	6.53LysIle: 6.53 ± 0.102	6.911LysLys: 6.911 ± 0.117	6.975LysLeu: 6.975 ± 0.102	1.858LysMet: 1.858 ± 0.044	5.55LysAsn: 5.55 ± 0.095	2.371LysPro: 2.371 ± 0.054	3.138LysGln: 3.138 ± 0.067	3.172LysArg: 3.172 ± 0.061	4.761LysSer: 4.761 ± 0.084	5.045LysThr: 5.045 ± 0.071	4.514LysVal: 4.514 ± 0.083	0.837LysTrp: 0.837 ± 0.027	3.073LysTyr: 3.073 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	5.233LeuAla: 5.233 ± 0.079	0.641LeuCys: 0.641 ± 0.025	5.06LeuAsp: 5.06 ± 0.076	5.998LeuGlu: 5.998 ± 0.092	5.123LeuPhe: 5.123 ± 0.084	5.669LeuGly: 5.669 ± 0.083	1.512LeuHis: 1.512 ± 0.045	8.248LeuIle: 8.248 ± 0.109	8.372LeuLys: 8.372 ± 0.126	8.754LeuLeu: 8.754 ± 0.12	1.916LeuMet: 1.916 ± 0.047	6.587LeuAsn: 6.587 ± 0.094	3.377LeuPro: 3.377 ± 0.06	3.102LeuGln: 3.102 ± 0.056	3.144LeuArg: 3.144 ± 0.058	6.822LeuSer: 6.822 ± 0.103	5.167LeuThr: 5.167 ± 0.095	4.889LeuVal: 4.889 ± 0.078	0.833LeuTrp: 0.833 ± 0.03	3.233LeuTyr: 3.233 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	1.259MetAla: 1.259 ± 0.039	0.139MetCys: 0.139 ± 0.013	0.914MetAsp: 0.914 ± 0.034	1.1MetGlu: 1.1 ± 0.033	0.838MetPhe: 0.838 ± 0.027	1.132MetGly: 1.132 ± 0.035	0.408MetHis: 0.408 ± 0.021	1.596MetIle: 1.596 ± 0.04	2.073MetLys: 2.073 ± 0.042	1.818MetLeu: 1.818 ± 0.048	0.481MetMet: 0.481 ± 0.024	1.243MetAsn: 1.243 ± 0.038	0.709MetPro: 0.709 ± 0.027	0.777MetGln: 0.777 ± 0.029	0.784MetArg: 0.784 ± 0.028	1.312MetSer: 1.312 ± 0.038	1.062MetThr: 1.062 ± 0.036	1.027MetVal: 1.027 ± 0.029	0.151MetTrp: 0.151 ± 0.012	0.627MetTyr: 0.627 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.952AsnAla: 3.952 ± 0.066	0.44AsnCys: 0.44 ± 0.023	3.77AsnAsp: 3.77 ± 0.069	4.176AsnGlu: 4.176 ± 0.07	3.251AsnPhe: 3.251 ± 0.075	4.188AsnGly: 4.188 ± 0.092	1.104AsnHis: 1.104 ± 0.033	5.827AsnIle: 5.827 ± 0.085	5.102AsnLys: 5.102 ± 0.081	5.902AsnLeu: 5.902 ± 0.095	1.257AsnMet: 1.257 ± 0.031	4.869AsnAsn: 4.869 ± 0.092	2.723AsnPro: 2.723 ± 0.073	2.318AsnGln: 2.318 ± 0.058	2.431AsnArg: 2.431 ± 0.047	4.371AsnSer: 4.371 ± 0.081	4.467AsnThr: 4.467 ± 0.091	3.85AsnVal: 3.85 ± 0.067	0.777AsnTrp: 0.777 ± 0.027	2.978AsnTyr: 2.978 ± 0.058	0.0AsnXaa: 0.0 ± 0.0
Pro	1.558ProAla: 1.558 ± 0.063	0.191ProCys: 0.191 ± 0.016	2.028ProAsp: 2.028 ± 0.056	2.63ProGlu: 2.63 ± 0.054	1.838ProPhe: 1.838 ± 0.044	1.816ProGly: 1.816 ± 0.069	0.585ProHis: 0.585 ± 0.024	2.991ProIle: 2.991 ± 0.062	2.616ProLys: 2.616 ± 0.055	2.832ProLeu: 2.832 ± 0.059	0.597ProMet: 0.597 ± 0.021	2.425ProAsn: 2.425 ± 0.075	0.782ProPro: 0.782 ± 0.035	1.056ProGln: 1.056 ± 0.034	0.957ProArg: 0.957 ± 0.031	2.154ProSer: 2.154 ± 0.048	1.793ProThr: 1.793 ± 0.048	2.008ProVal: 2.008 ± 0.045	0.292ProTrp: 0.292 ± 0.019	1.296ProTyr: 1.296 ± 0.038	0.0ProXaa: 0.0 ± 0.0
Gln	1.808GlnAla: 1.808 ± 0.047	0.206GlnCys: 0.206 ± 0.04	1.628GlnAsp: 1.628 ± 0.038	2.204GlnGlu: 2.204 ± 0.049	1.854GlnPhe: 1.854 ± 0.045	1.786GlnGly: 1.786 ± 0.041	0.541GlnHis: 0.541 ± 0.025	2.771GlnIle: 2.771 ± 0.058	2.645GlnLys: 2.645 ± 0.058	3.622GlnLeu: 3.622 ± 0.075	0.71GlnMet: 0.71 ± 0.025	2.266GlnAsn: 2.266 ± 0.048	1.041GlnPro: 1.041 ± 0.032	1.429GlnGln: 1.429 ± 0.043	1.27GlnArg: 1.27 ± 0.037	1.96GlnSer: 1.96 ± 0.05	1.788GlnThr: 1.788 ± 0.049	1.926GlnVal: 1.926 ± 0.075	0.348GlnTrp: 0.348 ± 0.02	1.279GlnTyr: 1.279 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	2.027ArgAla: 2.027 ± 0.051	0.172ArgCys: 0.172 ± 0.015	1.875ArgAsp: 1.875 ± 0.043	2.233ArgGlu: 2.233 ± 0.047	2.011ArgPhe: 2.011 ± 0.045	2.041ArgGly: 2.041 ± 0.053	0.554ArgHis: 0.554 ± 0.021	3.034ArgIle: 3.034 ± 0.058	2.636ArgLys: 2.636 ± 0.06	3.343ArgLeu: 3.343 ± 0.054	0.771ArgMet: 0.771 ± 0.028	2.249ArgAsn: 2.249 ± 0.047	1.041ArgPro: 1.041 ± 0.029	1.088ArgGln: 1.088 ± 0.038	1.371ArgArg: 1.371 ± 0.045	2.054ArgSer: 2.054 ± 0.049	1.901ArgThr: 1.901 ± 0.045	2.153ArgVal: 2.153 ± 0.045	0.372ArgTrp: 0.372 ± 0.021	1.543ArgTyr: 1.543 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	3.51SerAla: 3.51 ± 0.107	0.63SerCys: 0.63 ± 0.04	3.52SerAsp: 3.52 ± 0.084	4.069SerGlu: 4.069 ± 0.068	3.919SerPhe: 3.919 ± 0.058	4.473SerGly: 4.473 ± 0.08	1.028SerHis: 1.028 ± 0.034	6.167SerIle: 6.167 ± 0.087	5.444SerLys: 5.444 ± 0.088	6.06SerLeu: 6.06 ± 0.089	1.114SerMet: 1.114 ± 0.034	4.45SerAsn: 4.45 ± 0.076	2.141SerPro: 2.141 ± 0.065	2.255SerGln: 2.255 ± 0.047	2.263SerArg: 2.263 ± 0.051	4.255SerSer: 4.255 ± 0.079	3.694SerThr: 3.694 ± 0.116	3.662SerVal: 3.662 ± 0.061	0.648SerTrp: 0.648 ± 0.028	2.697SerTyr: 2.697 ± 0.063	0.0SerXaa: 0.0 ± 0.0
Thr	3.435ThrAla: 3.435 ± 0.079	0.314ThrCys: 0.314 ± 0.019	3.044ThrAsp: 3.044 ± 0.068	3.43ThrGlu: 3.43 ± 0.072	3.11ThrPhe: 3.11 ± 0.066	3.719ThrGly: 3.719 ± 0.084	1.071ThrHis: 1.071 ± 0.033	5.896ThrIle: 5.896 ± 0.191	4.065ThrLys: 4.065 ± 0.069	5.813ThrLeu: 5.813 ± 0.082	0.899ThrMet: 0.899 ± 0.035	3.697ThrAsn: 3.697 ± 0.072	2.522ThrPro: 2.522 ± 0.069	1.934ThrGln: 1.934 ± 0.063	1.731ThrArg: 1.731 ± 0.042	4.163ThrSer: 4.163 ± 0.111	3.677ThrThr: 3.677 ± 0.142	3.537ThrVal: 3.537 ± 0.082	0.572ThrTrp: 0.572 ± 0.029	2.423ThrTyr: 2.423 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	3.452ValAla: 3.452 ± 0.062	0.501ValCys: 0.501 ± 0.033	3.32ValAsp: 3.32 ± 0.078	3.689ValGlu: 3.689 ± 0.064	3.478ValPhe: 3.478 ± 0.071	3.336ValGly: 3.336 ± 0.064	0.905ValHis: 0.905 ± 0.033	5.309ValIle: 5.309 ± 0.08	4.236ValLys: 4.236 ± 0.074	5.655ValLeu: 5.655 ± 0.082	1.167ValMet: 1.167 ± 0.04	3.786ValAsn: 3.786 ± 0.076	1.839ValPro: 1.839 ± 0.046	1.589ValGln: 1.589 ± 0.044	1.892ValArg: 1.892 ± 0.049	4.201ValSer: 4.201 ± 0.067	3.558ValThr: 3.558 ± 0.086	3.574ValVal: 3.574 ± 0.065	0.523ValTrp: 0.523 ± 0.021	2.19ValTyr: 2.19 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	0.517TrpAla: 0.517 ± 0.024	0.086TrpCys: 0.086 ± 0.009	0.525TrpAsp: 0.525 ± 0.023	0.624TrpGlu: 0.624 ± 0.024	0.577TrpPhe: 0.577 ± 0.026	0.615TrpGly: 0.615 ± 0.025	0.204TrpHis: 0.204 ± 0.014	0.742TrpIle: 0.742 ± 0.028	0.844TrpLys: 0.844 ± 0.031	0.93TrpLeu: 0.93 ± 0.032	0.294TrpMet: 0.294 ± 0.018	0.69TrpAsn: 0.69 ± 0.026	0.196TrpPro: 0.196 ± 0.015	0.35TrpGln: 0.35 ± 0.019	0.378TrpArg: 0.378 ± 0.022	0.657TrpSer: 0.657 ± 0.029	0.566TrpThr: 0.566 ± 0.029	0.632TrpVal: 0.632 ± 0.025	0.131TrpTrp: 0.131 ± 0.011	0.447TrpTyr: 0.447 ± 0.044	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.272TyrAla: 2.272 ± 0.047	0.293TyrCys: 0.293 ± 0.017	2.214TyrAsp: 2.214 ± 0.048	2.182TyrGlu: 2.182 ± 0.049	2.404TyrPhe: 2.404 ± 0.061	2.483TyrGly: 2.483 ± 0.055	0.794TyrHis: 0.794 ± 0.031	3.199TyrIle: 3.199 ± 0.06	3.348TyrLys: 3.348 ± 0.072	3.807TyrLeu: 3.807 ± 0.072	0.626TyrMet: 0.626 ± 0.025	2.857TyrAsn: 2.857 ± 0.06	1.313TyrPro: 1.313 ± 0.035	1.398TyrGln: 1.398 ± 0.043	1.645TyrArg: 1.645 ± 0.044	2.552TyrSer: 2.552 ± 0.061	2.385TyrThr: 2.385 ± 0.063	2.107TyrVal: 2.107 ± 0.049	0.446TyrTrp: 0.446 ± 0.021	1.855TyrTyr: 1.855 ± 0.051	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3010 proteins (1019847 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---