Cycloclasticus sp. (strain P1)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales; Piscirickettsiaceae; Cycloclasticus; unclassified Cycloclasticus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2247 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0C0P0|K0C0P0_CYCSP UvrABC system protein B OS=Cycloclasticus sp. (strain P1) OX=385025 GN=uvrB PE=3 SV=1
MM1 pKa = 7.75KK2 pKa = 10.16LNKK5 pKa = 9.48IQPSLNNQKK14 pKa = 10.15GVATLLVSIILLIGVTLITVFAARR38 pKa = 11.84VGVMDD43 pKa = 3.78QRR45 pKa = 11.84ISANEE50 pKa = 3.76YY51 pKa = 8.71RR52 pKa = 11.84HH53 pKa = 6.5KK54 pKa = 10.28EE55 pKa = 3.79AQAAADD61 pKa = 3.58AALEE65 pKa = 3.86QAAAFINEE73 pKa = 3.95NTGLYY78 pKa = 10.32DD79 pKa = 3.39GTVGGTYY86 pKa = 9.9PWVEE90 pKa = 4.02CVGGYY95 pKa = 8.65TSLFPCTNGSDD106 pKa = 4.17TYY108 pKa = 11.81NLAYY112 pKa = 10.71DD113 pKa = 4.0NDD115 pKa = 4.28LTTLTTIEE123 pKa = 4.3PLQLGATPTAVDD135 pKa = 4.28LSSGVVSNTYY145 pKa = 8.48LTYY148 pKa = 9.51TNSGNVMTAIGTGEE162 pKa = 4.12SLDD165 pKa = 3.92GTGNAYY171 pKa = 10.25AQISYY176 pKa = 8.06TQITLLTSEE185 pKa = 4.6EE186 pKa = 4.44VPPIMTPIIEE196 pKa = 4.45LGGTFTIVADD206 pKa = 4.06PNGGGKK212 pKa = 9.75GVPVSAWVATFDD224 pKa = 3.55TSGGLGSWQTCQLDD238 pKa = 3.57AFLDD242 pKa = 3.83NSGDD246 pKa = 3.57ICSEE250 pKa = 4.07TLDD253 pKa = 4.93DD254 pKa = 3.6TDD256 pKa = 2.8SWKK259 pKa = 10.84FCDD262 pKa = 4.5CDD264 pKa = 3.76GDD266 pKa = 4.15KK267 pKa = 10.99LSEE270 pKa = 4.29PGKK273 pKa = 9.88VNNDD277 pKa = 2.45IVQADD282 pKa = 3.99PGDD285 pKa = 4.15FPASVLSYY293 pKa = 10.04LFPNFDD299 pKa = 3.36SFQDD303 pKa = 3.6VVNIADD309 pKa = 3.97VEE311 pKa = 4.29LTNCSGLQALASGWSTSKK329 pKa = 10.54IVTVDD334 pKa = 3.88GDD336 pKa = 3.87CTMPSNSIIGSRR348 pKa = 11.84DD349 pKa = 3.0GPLILVVSGHH359 pKa = 6.45LKK361 pKa = 10.17INANSDD367 pKa = 3.63FYY369 pKa = 11.49GIALGLSDD377 pKa = 3.62VTLNGGVKK385 pKa = 9.15VHH387 pKa = 6.63GSLLAEE393 pKa = 5.16DD394 pKa = 3.99STKK397 pKa = 10.31LTTGGYY403 pKa = 8.81AQVYY407 pKa = 10.29DD408 pKa = 4.17EE409 pKa = 5.31FVLGSIVEE417 pKa = 4.23DD418 pKa = 4.09LSNLGLAKK426 pKa = 10.03QKK428 pKa = 10.95YY429 pKa = 8.45SWTDD433 pKa = 2.98IQPP436 pKa = 3.25

Molecular weight: 45.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0C4V4|K0C4V4_CYCSP Replicative DNA helicase OS=Cycloclasticus sp. (strain P1) OX=385025 GN=dnaB PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.08RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.27SGRR28 pKa = 11.84KK29 pKa = 8.85IINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2247

0

2247

715765

38

1645

318.5

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.419 ± 0.049

1.017 ± 0.017

5.647 ± 0.045

6.291 ± 0.057

4.056 ± 0.036

6.917 ± 0.049

2.262 ± 0.028

6.747 ± 0.043

5.777 ± 0.048

10.491 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.576 ± 0.024

4.291 ± 0.031

3.93 ± 0.031

4.22 ± 0.042

4.438 ± 0.041

6.528 ± 0.044

5.418 ± 0.041

6.959 ± 0.048

1.144 ± 0.02

2.87 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski