Amino acid dipepetide frequency for Cycloclasticus sp. (strain P1)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.201AlaAla: 7.201 ± 0.122	1.025AlaCys: 1.025 ± 0.042	4.865AlaAsp: 4.865 ± 0.101	5.273AlaGlu: 5.273 ± 0.098	3.346AlaPhe: 3.346 ± 0.071	6.164AlaGly: 6.164 ± 0.11	1.72AlaHis: 1.72 ± 0.053	6.012AlaIle: 6.012 ± 0.108	4.841AlaLys: 4.841 ± 0.1	9.403AlaLeu: 9.403 ± 0.139	2.376AlaMet: 2.376 ± 0.058	3.536AlaAsn: 3.536 ± 0.067	2.597AlaPro: 2.597 ± 0.059	3.252AlaGln: 3.252 ± 0.076	3.511AlaArg: 3.511 ± 0.078	5.505AlaSer: 5.505 ± 0.08	4.348AlaThr: 4.348 ± 0.084	5.893AlaVal: 5.893 ± 0.097	0.936AlaTrp: 0.936 ± 0.039	2.388AlaTyr: 2.388 ± 0.064	0.0AlaXaa: 0.0 ± 0.0
Cys	0.781CysAla: 0.781 ± 0.038	0.147CysCys: 0.147 ± 0.016	0.597CysAsp: 0.597 ± 0.032	0.609CysGlu: 0.609 ± 0.034	0.471CysPhe: 0.471 ± 0.027	0.897CysGly: 0.897 ± 0.041	0.317CysHis: 0.317 ± 0.02	0.641CysIle: 0.641 ± 0.028	0.44CysLys: 0.44 ± 0.024	1.039CysLeu: 1.039 ± 0.038	0.228CysMet: 0.228 ± 0.022	0.306CysAsn: 0.306 ± 0.019	0.493CysPro: 0.493 ± 0.028	0.457CysGln: 0.457 ± 0.026	0.435CysArg: 0.435 ± 0.027	0.671CysSer: 0.671 ± 0.035	0.516CysThr: 0.516 ± 0.026	0.682CysVal: 0.682 ± 0.035	0.12CysTrp: 0.12 ± 0.015	0.32CysTyr: 0.32 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	4.45AspAla: 4.45 ± 0.086	0.556AspCys: 0.556 ± 0.028	3.342AspAsp: 3.342 ± 0.073	4.332AspGlu: 4.332 ± 0.099	2.501AspPhe: 2.501 ± 0.063	3.712AspGly: 3.712 ± 0.082	1.132AspHis: 1.132 ± 0.044	4.506AspIle: 4.506 ± 0.089	3.275AspLys: 3.275 ± 0.066	5.396AspLeu: 5.396 ± 0.092	1.452AspMet: 1.452 ± 0.054	2.458AspAsn: 2.458 ± 0.054	2.11AspPro: 2.11 ± 0.059	2.038AspGln: 2.038 ± 0.048	2.344AspArg: 2.344 ± 0.061	3.269AspSer: 3.269 ± 0.07	2.835AspThr: 2.835 ± 0.065	4.109AspVal: 4.109 ± 0.078	0.805AspTrp: 0.805 ± 0.035	1.851AspTyr: 1.851 ± 0.056	0.0AspXaa: 0.0 ± 0.0
Glu	5.345GluAla: 5.345 ± 0.109	0.485GluCys: 0.485 ± 0.027	2.921GluAsp: 2.921 ± 0.068	4.014GluGlu: 4.014 ± 0.108	2.328GluPhe: 2.328 ± 0.056	4.035GluGly: 4.035 ± 0.089	1.572GluHis: 1.572 ± 0.054	3.845GluIle: 3.845 ± 0.081	4.553GluLys: 4.553 ± 0.1	6.829GluLeu: 6.829 ± 0.099	1.738GluMet: 1.738 ± 0.052	2.664GluAsn: 2.664 ± 0.065	2.075GluPro: 2.075 ± 0.051	3.667GluGln: 3.667 ± 0.078	3.22GluArg: 3.22 ± 0.069	3.561GluSer: 3.561 ± 0.075	3.396GluThr: 3.396 ± 0.082	4.313GluVal: 4.313 ± 0.082	0.683GluTrp: 0.683 ± 0.032	1.667GluTyr: 1.667 ± 0.049	0.0GluXaa: 0.0 ± 0.0
Phe	3.222PheAla: 3.222 ± 0.07	0.509PheCys: 0.509 ± 0.026	2.642PheAsp: 2.642 ± 0.05	2.511PheGlu: 2.511 ± 0.058	1.973PhePhe: 1.973 ± 0.059	2.931PheGly: 2.931 ± 0.072	0.805PheHis: 0.805 ± 0.037	3.011PheIle: 3.011 ± 0.068	2.263PheLys: 2.263 ± 0.057	3.679PheLeu: 3.679 ± 0.08	1.023PheMet: 1.023 ± 0.04	2.03PheAsn: 2.03 ± 0.056	1.484PhePro: 1.484 ± 0.045	1.27PheGln: 1.27 ± 0.047	1.337PheArg: 1.337 ± 0.046	3.315PheSer: 3.315 ± 0.07	2.091PheThr: 2.091 ± 0.054	2.59PheVal: 2.59 ± 0.058	0.465PheTrp: 0.465 ± 0.026	1.407PheTyr: 1.407 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	5.327GlyAla: 5.327 ± 0.111	0.863GlyCys: 0.863 ± 0.043	3.741GlyAsp: 3.741 ± 0.078	4.422GlyGlu: 4.422 ± 0.074	3.18GlyPhe: 3.18 ± 0.069	4.946GlyGly: 4.946 ± 0.108	1.792GlyHis: 1.792 ± 0.054	4.784GlyIle: 4.784 ± 0.083	3.87GlyLys: 3.87 ± 0.086	7.582GlyLeu: 7.582 ± 0.116	1.974GlyMet: 1.974 ± 0.048	2.351GlyAsn: 2.351 ± 0.06	1.918GlyPro: 1.918 ± 0.06	2.856GlyGln: 2.856 ± 0.069	3.04GlyArg: 3.04 ± 0.068	4.127GlySer: 4.127 ± 0.079	3.43GlyThr: 3.43 ± 0.089	5.657GlyVal: 5.657 ± 0.1	0.995GlyTrp: 0.995 ± 0.039	2.318GlyTyr: 2.318 ± 0.057	0.0GlyXaa: 0.0 ± 0.0
His	1.822HisAla: 1.822 ± 0.053	0.307HisCys: 0.307 ± 0.022	1.123HisAsp: 1.123 ± 0.043	1.295HisGlu: 1.295 ± 0.043	1.058HisPhe: 1.058 ± 0.04	1.589HisGly: 1.589 ± 0.053	0.713HisHis: 0.713 ± 0.032	1.436HisIle: 1.436 ± 0.045	1.157HisLys: 1.157 ± 0.045	2.3HisLeu: 2.3 ± 0.062	0.541HisMet: 0.541 ± 0.03	0.849HisAsn: 0.849 ± 0.039	1.182HisPro: 1.182 ± 0.046	1.129HisGln: 1.129 ± 0.043	1.059HisArg: 1.059 ± 0.043	1.253HisSer: 1.253 ± 0.05	1.119HisThr: 1.119 ± 0.04	1.475HisVal: 1.475 ± 0.045	0.349HisTrp: 0.349 ± 0.024	0.863HisTyr: 0.863 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	6.117IleAla: 6.117 ± 0.093	0.697IleCys: 0.697 ± 0.028	4.57IleAsp: 4.57 ± 0.094	4.866IleGlu: 4.866 ± 0.093	2.438IlePhe: 2.438 ± 0.069	4.915IleGly: 4.915 ± 0.09	1.376IleHis: 1.376 ± 0.045	4.344IleIle: 4.344 ± 0.106	4.246IleLys: 4.246 ± 0.086	5.566IleLeu: 5.566 ± 0.097	1.453IleMet: 1.453 ± 0.047	3.578IleAsn: 3.578 ± 0.077	2.829IlePro: 2.829 ± 0.071	2.529IleGln: 2.529 ± 0.063	2.931IleArg: 2.931 ± 0.064	4.587IleSer: 4.587 ± 0.087	3.81IleThr: 3.81 ± 0.086	4.33IleVal: 4.33 ± 0.08	0.574IleTrp: 0.574 ± 0.029	1.718IleTyr: 1.718 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	5.133LysAla: 5.133 ± 0.088	0.334LysCys: 0.334 ± 0.026	2.995LysAsp: 2.995 ± 0.072	3.817LysGlu: 3.817 ± 0.09	1.5LysPhe: 1.5 ± 0.047	3.883LysGly: 3.883 ± 0.086	1.361LysHis: 1.361 ± 0.046	3.544LysIle: 3.544 ± 0.076	4.328LysLys: 4.328 ± 0.091	5.436LysLeu: 5.436 ± 0.093	1.482LysMet: 1.482 ± 0.045	2.864LysAsn: 2.864 ± 0.064	2.684LysPro: 2.684 ± 0.073	2.998LysGln: 2.998 ± 0.067	2.909LysArg: 2.909 ± 0.062	3.3LysSer: 3.3 ± 0.071	3.558LysThr: 3.558 ± 0.079	3.832LysVal: 3.832 ± 0.075	0.535LysTrp: 0.535 ± 0.03	1.28LysTyr: 1.28 ± 0.045	0.0LysXaa: 0.0 ± 0.0
Leu	9.503LeuAla: 9.503 ± 0.126	0.991LeuCys: 0.991 ± 0.036	6.122LeuAsp: 6.122 ± 0.101	6.115LeuGlu: 6.115 ± 0.105	4.12LeuPhe: 4.12 ± 0.082	7.097LeuGly: 7.097 ± 0.128	2.086LeuHis: 2.086 ± 0.057	6.811LeuIle: 6.811 ± 0.117	6.17LeuLys: 6.17 ± 0.099	10.962LeuLeu: 10.962 ± 0.174	2.763LeuMet: 2.763 ± 0.06	4.834LeuAsn: 4.834 ± 0.084	4.617LeuPro: 4.617 ± 0.083	3.712LeuGln: 3.712 ± 0.083	4.437LeuArg: 4.437 ± 0.084	8.174LeuSer: 8.174 ± 0.124	6.11LeuThr: 6.11 ± 0.101	6.677LeuVal: 6.677 ± 0.098	0.999LeuTrp: 0.999 ± 0.041	2.61LeuTyr: 2.61 ± 0.057	0.0LeuXaa: 0.0 ± 0.0
Met	2.421MetAla: 2.421 ± 0.059	0.222MetCys: 0.222 ± 0.019	1.415MetAsp: 1.415 ± 0.049	1.292MetGlu: 1.292 ± 0.051	0.865MetPhe: 0.865 ± 0.037	1.941MetGly: 1.941 ± 0.047	0.528MetHis: 0.528 ± 0.03	1.485MetIle: 1.485 ± 0.048	1.499MetLys: 1.499 ± 0.047	2.752MetLeu: 2.752 ± 0.07	0.759MetMet: 0.759 ± 0.035	1.168MetAsn: 1.168 ± 0.045	1.27MetPro: 1.27 ± 0.048	1.038MetGln: 1.038 ± 0.046	1.116MetArg: 1.116 ± 0.036	2.075MetSer: 2.075 ± 0.061	1.503MetThr: 1.503 ± 0.047	1.769MetVal: 1.769 ± 0.048	0.182MetTrp: 0.182 ± 0.017	0.461MetTyr: 0.461 ± 0.027	0.0MetXaa: 0.0 ± 0.0
Asn	3.512AsnAla: 3.512 ± 0.071	0.4AsnCys: 0.4 ± 0.024	2.445AsnAsp: 2.445 ± 0.062	2.811AsnGlu: 2.811 ± 0.061	1.499AsnPhe: 1.499 ± 0.043	2.907AsnGly: 2.907 ± 0.065	0.9AsnHis: 0.9 ± 0.037	3.313AsnIle: 3.313 ± 0.073	2.945AsnLys: 2.945 ± 0.061	3.842AsnLeu: 3.842 ± 0.08	0.998AsnMet: 0.998 ± 0.033	2.11AsnAsn: 2.11 ± 0.058	2.105AsnPro: 2.105 ± 0.051	1.819AsnGln: 1.819 ± 0.061	1.955AsnArg: 1.955 ± 0.061	2.382AsnSer: 2.382 ± 0.065	2.452AsnThr: 2.452 ± 0.053	2.688AsnVal: 2.688 ± 0.067	0.58AsnTrp: 0.58 ± 0.031	1.25AsnTyr: 1.25 ± 0.042	0.0AsnXaa: 0.0 ± 0.0
Pro	3.271ProAla: 3.271 ± 0.081	0.337ProCys: 0.337 ± 0.023	2.495ProAsp: 2.495 ± 0.058	2.898ProGlu: 2.898 ± 0.061	1.72ProPhe: 1.72 ± 0.057	2.459ProGly: 2.459 ± 0.057	0.812ProHis: 0.812 ± 0.034	2.791ProIle: 2.791 ± 0.071	1.97ProLys: 1.97 ± 0.059	4.022ProLeu: 4.022 ± 0.086	1.016ProMet: 1.016 ± 0.036	1.833ProAsn: 1.833 ± 0.054	1.222ProPro: 1.222 ± 0.04	1.277ProGln: 1.277 ± 0.039	1.405ProArg: 1.405 ± 0.046	2.624ProSer: 2.624 ± 0.057	2.283ProThr: 2.283 ± 0.06	3.19ProVal: 3.19 ± 0.068	0.457ProTrp: 0.457 ± 0.028	1.22ProTyr: 1.22 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	4.126GlnAla: 4.126 ± 0.087	0.407GlnCys: 0.407 ± 0.024	1.653GlnAsp: 1.653 ± 0.047	2.188GlnGlu: 2.188 ± 0.058	1.587GlnPhe: 1.587 ± 0.044	2.414GlnGly: 2.414 ± 0.061	1.351GlnHis: 1.351 ± 0.047	2.425GlnIle: 2.425 ± 0.062	2.075GlnLys: 2.075 ± 0.054	4.997GlnLeu: 4.997 ± 0.106	1.013GlnMet: 1.013 ± 0.04	1.512GlnAsn: 1.512 ± 0.051	1.484GlnPro: 1.484 ± 0.047	3.011GlnGln: 3.011 ± 0.087	2.374GlnArg: 2.374 ± 0.066	2.53GlnSer: 2.53 ± 0.066	2.295GlnThr: 2.295 ± 0.06	2.843GlnVal: 2.843 ± 0.069	0.64GlnTrp: 0.64 ± 0.032	1.28GlnTyr: 1.28 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	3.331ArgAla: 3.331 ± 0.073	0.465ArgCys: 0.465 ± 0.027	2.428ArgAsp: 2.428 ± 0.064	2.817ArgGlu: 2.817 ± 0.065	2.199ArgPhe: 2.199 ± 0.064	2.687ArgGly: 2.687 ± 0.07	1.084ArgHis: 1.084 ± 0.039	2.956ArgIle: 2.956 ± 0.062	2.349ArgLys: 2.349 ± 0.055	5.08ArgLeu: 5.08 ± 0.107	1.176ArgMet: 1.176 ± 0.039	1.635ArgAsn: 1.635 ± 0.043	1.625ArgPro: 1.625 ± 0.045	2.189ArgGln: 2.189 ± 0.057	2.263ArgArg: 2.263 ± 0.069	2.638ArgSer: 2.638 ± 0.061	2.016ArgThr: 2.016 ± 0.056	3.218ArgVal: 3.218 ± 0.077	0.567ArgTrp: 0.567 ± 0.028	1.661ArgTyr: 1.661 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	5.308SerAla: 5.308 ± 0.096	0.683SerCys: 0.683 ± 0.032	3.544SerAsp: 3.544 ± 0.07	3.746SerGlu: 3.746 ± 0.072	2.863SerPhe: 2.863 ± 0.067	4.883SerGly: 4.883 ± 0.097	1.408SerHis: 1.408 ± 0.042	4.697SerIle: 4.697 ± 0.077	3.391SerLys: 3.391 ± 0.083	7.278SerLeu: 7.278 ± 0.123	1.758SerMet: 1.758 ± 0.048	2.703SerAsn: 2.703 ± 0.061	2.46SerPro: 2.46 ± 0.054	2.369SerGln: 2.369 ± 0.061	2.791SerArg: 2.791 ± 0.062	4.415SerSer: 4.415 ± 0.076	3.691SerThr: 3.691 ± 0.073	4.623SerVal: 4.623 ± 0.106	0.795SerTrp: 0.795 ± 0.037	1.869SerTyr: 1.869 ± 0.049	0.0SerXaa: 0.0 ± 0.0
Thr	4.455ThrAla: 4.455 ± 0.084	0.49ThrCys: 0.49 ± 0.027	3.026ThrAsp: 3.026 ± 0.078	3.116ThrGlu: 3.116 ± 0.066	2.152ThrPhe: 2.152 ± 0.061	4.374ThrGly: 4.374 ± 0.095	1.326ThrHis: 1.326 ± 0.046	3.525ThrIle: 3.525 ± 0.065	2.575ThrLys: 2.575 ± 0.056	6.547ThrLeu: 6.547 ± 0.119	1.222ThrMet: 1.222 ± 0.043	2.001ThrAsn: 2.001 ± 0.059	2.738ThrPro: 2.738 ± 0.068	2.185ThrGln: 2.185 ± 0.061	2.244ThrArg: 2.244 ± 0.058	3.271ThrSer: 3.271 ± 0.073	3.001ThrThr: 3.001 ± 0.085	3.961ThrVal: 3.961 ± 0.098	0.559ThrTrp: 0.559 ± 0.025	1.411ThrTyr: 1.411 ± 0.048	0.0ThrXaa: 0.0 ± 0.0
Val	5.852ValAla: 5.852 ± 0.103	0.77ValCys: 0.77 ± 0.034	4.325ValAsp: 4.325 ± 0.087	4.624ValGlu: 4.624 ± 0.099	2.935ValPhe: 2.935 ± 0.068	4.643ValGly: 4.643 ± 0.101	1.323ValHis: 1.323 ± 0.05	5.024ValIle: 5.024 ± 0.101	3.721ValLys: 3.721 ± 0.087	7.35ValLeu: 7.35 ± 0.108	1.861ValMet: 1.861 ± 0.055	3.06ValAsn: 3.06 ± 0.069	2.682ValPro: 2.682 ± 0.064	2.103ValGln: 2.103 ± 0.054	2.9ValArg: 2.9 ± 0.065	4.956ValSer: 4.956 ± 0.089	3.822ValThr: 3.822 ± 0.093	5.069ValVal: 5.069 ± 0.097	0.672ValTrp: 0.672 ± 0.029	1.9ValTyr: 1.9 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	0.882TrpAla: 0.882 ± 0.037	0.143TrpCys: 0.143 ± 0.014	0.581TrpAsp: 0.581 ± 0.03	0.592TrpGlu: 0.592 ± 0.026	0.566TrpPhe: 0.566 ± 0.032	0.686TrpGly: 0.686 ± 0.032	0.277TrpHis: 0.277 ± 0.018	0.604TrpIle: 0.604 ± 0.035	0.574TrpLys: 0.574 ± 0.032	1.568TrpLeu: 1.568 ± 0.051	0.305TrpMet: 0.305 ± 0.02	0.412TrpAsn: 0.412 ± 0.024	0.447TrpPro: 0.447 ± 0.025	0.686TrpGln: 0.686 ± 0.03	0.549TrpArg: 0.549 ± 0.028	0.768TrpSer: 0.768 ± 0.036	0.447TrpThr: 0.447 ± 0.028	0.929TrpVal: 0.929 ± 0.037	0.145TrpTrp: 0.145 ± 0.017	0.282TrpTyr: 0.282 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.135TyrAla: 2.135 ± 0.055	0.335TyrCys: 0.335 ± 0.025	1.639TyrAsp: 1.639 ± 0.051	1.575TyrGlu: 1.575 ± 0.053	1.257TyrPhe: 1.257 ± 0.043	2.012TyrGly: 2.012 ± 0.056	0.738TyrHis: 0.738 ± 0.033	1.72TyrIle: 1.72 ± 0.048	1.534TyrLys: 1.534 ± 0.047	3.283TyrLeu: 3.283 ± 0.07	0.623TyrMet: 0.623 ± 0.027	1.009TyrAsn: 1.009 ± 0.036	1.277TyrPro: 1.277 ± 0.043	1.618TyrGln: 1.618 ± 0.053	1.562TyrArg: 1.562 ± 0.046	1.855TyrSer: 1.855 ± 0.057	1.454TyrThr: 1.454 ± 0.052	1.746TyrVal: 1.746 ± 0.05	0.384TyrTrp: 0.384 ± 0.022	0.946TyrTyr: 0.946 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2247 proteins (715765 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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