Synechococcus phage S-SSM4
Average proteome isoelectric point is 5.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 220 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M1U9C4|M1U9C4_9CAUD Uncharacterized protein OS=Synechococcus phage S-SSM4 OX=536466 GN=CYXG_00037 PE=4 SV=1
MM1 pKa = 7.77 TDD3 pKa = 3.73 FFDD6 pKa = 5.88 NEE8 pKa = 4.31 TSDD11 pKa = 3.64 QQHH14 pKa = 6.83 SIDD17 pKa = 4.32 NMGDD21 pKa = 3.46 SLIEE25 pKa = 3.89 AMRR28 pKa = 11.84 VAIGQGRR35 pKa = 11.84 IEE37 pKa = 4.44 DD38 pKa = 3.72 AASISDD44 pKa = 3.37 EE45 pKa = 4.67 WITPDD50 pKa = 5.63 CNDD53 pKa = 3.67 PCNGEE58 pKa = 4.02 YY59 pKa = 10.79 EE60 pKa = 5.12 FIFLKK65 pKa = 11.04 NNTFGDD71 pKa = 4.21 WTWDD75 pKa = 3.12 ACASS79 pKa = 3.6
Molecular weight: 8.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.81
IPC2_protein 3.579
IPC_protein 3.541
Toseland 3.325
ProMoST 3.732
Dawson 3.554
Bjellqvist 3.719
Wikipedia 3.516
Rodwell 3.376
Grimsley 3.249
Solomon 3.516
Lehninger 3.478
Nozaki 3.681
DTASelect 3.91
Thurlkill 3.414
EMBOSS 3.528
Sillero 3.668
Patrickios 1.825
IPC_peptide 3.516
IPC2_peptide 3.63
IPC2.peptide.svr19 3.664
Protein with the highest isoelectric point:
>tr|M1UFX2|M1UFX2_9CAUD Uncharacterized protein OS=Synechococcus phage S-SSM4 OX=536466 GN=CYXG_00048 PE=4 SV=1
MM1 pKa = 7.87 AKK3 pKa = 10.14 NQMKK7 pKa = 10.11 QMKK10 pKa = 10.2 KK11 pKa = 9.88 LMQRR15 pKa = 11.84 NGFEE19 pKa = 4.19 LTRR22 pKa = 11.84 EE23 pKa = 4.19 TKK25 pKa = 10.49 HH26 pKa = 6.56 FIWKK30 pKa = 9.47 HH31 pKa = 3.45 KK32 pKa = 8.65 TGVVMTTSKK41 pKa = 9.89 TPSDD45 pKa = 3.79 NYY47 pKa = 11.18 AIAQASRR54 pKa = 11.84 QIRR57 pKa = 11.84 RR58 pKa = 11.84 TIGKK62 pKa = 8.92 VVV64 pKa = 2.82
Molecular weight: 7.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.242
IPC2_protein 10.087
IPC_protein 10.95
Toseland 11.374
ProMoST 11.316
Dawson 11.418
Bjellqvist 11.169
Wikipedia 11.667
Rodwell 11.754
Grimsley 11.433
Solomon 11.652
Lehninger 11.608
Nozaki 11.359
DTASelect 11.169
Thurlkill 11.359
EMBOSS 11.798
Sillero 11.359
Patrickios 11.506
IPC_peptide 11.667
IPC2_peptide 10.116
IPC2.peptide.svr19 8.494
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
220
0
220
57723
47
6932
262.4
29.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.788 ± 0.232
0.884 ± 0.088
6.89 ± 0.116
6.176 ± 0.276
4.366 ± 0.098
7.688 ± 0.33
1.571 ± 0.137
6.211 ± 0.202
6.238 ± 0.417
7.11 ± 0.19
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.107 ± 0.221
5.966 ± 0.203
4.149 ± 0.153
3.829 ± 0.085
3.898 ± 0.151
6.808 ± 0.171
7.224 ± 0.381
6.637 ± 0.204
1.228 ± 0.098
4.232 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here