Amino acid dipepetide frequency for Synechococcus phage S-SSM4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.838AlaAla: 5.838 ± 0.541
0.45AlaCys: 0.45 ± 0.124
4.037AlaAsp: 4.037 ± 0.339
4.002AlaGlu: 4.002 ± 0.296
2.685AlaPhe: 2.685 ± 0.23
6.462AlaGly: 6.462 ± 0.593
0.849AlaHis: 0.849 ± 0.129
3.777AlaIle: 3.777 ± 0.261
4.037AlaLys: 4.037 ± 0.368
4.643AlaLeu: 4.643 ± 0.292
1.507AlaMet: 1.507 ± 0.226
3.725AlaAsn: 3.725 ± 0.306
2.772AlaPro: 2.772 ± 0.235
2.772AlaGln: 2.772 ± 0.22
2.616AlaArg: 2.616 ± 0.269
4.972AlaSer: 4.972 ± 0.301
5.682AlaThr: 5.682 ± 0.622
4.002AlaVal: 4.002 ± 0.294
0.762AlaTrp: 0.762 ± 0.152
2.287AlaTyr: 2.287 ± 0.2
0.0AlaXaa: 0.0 ± 0.0
Cys
0.45CysAla: 0.45 ± 0.101
0.069CysCys: 0.069 ± 0.05
0.797CysAsp: 0.797 ± 0.134
0.606CysGlu: 0.606 ± 0.126
0.416CysPhe: 0.416 ± 0.095
0.572CysGly: 0.572 ± 0.102
0.225CysHis: 0.225 ± 0.077
0.433CysIle: 0.433 ± 0.084
0.468CysLys: 0.468 ± 0.105
0.502CysLeu: 0.502 ± 0.109
0.26CysMet: 0.26 ± 0.077
0.416CysAsn: 0.416 ± 0.08
0.364CysPro: 0.364 ± 0.086
0.295CysGln: 0.295 ± 0.075
0.433CysArg: 0.433 ± 0.091
0.658CysSer: 0.658 ± 0.118
0.745CysThr: 0.745 ± 0.13
0.52CysVal: 0.52 ± 0.107
0.156CysTrp: 0.156 ± 0.05
0.45CysTyr: 0.45 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
4.643AspAla: 4.643 ± 0.297
0.71AspCys: 0.71 ± 0.113
4.868AspAsp: 4.868 ± 0.359
4.227AspGlu: 4.227 ± 0.377
3.118AspPhe: 3.118 ± 0.231
5.977AspGly: 5.977 ± 0.379
1.091AspHis: 1.091 ± 0.157
4.227AspIle: 4.227 ± 0.281
3.725AspLys: 3.725 ± 0.28
5.388AspLeu: 5.388 ± 0.364
1.421AspMet: 1.421 ± 0.163
3.846AspAsn: 3.846 ± 0.308
3.846AspPro: 3.846 ± 0.359
2.408AspGln: 2.408 ± 0.185
2.668AspArg: 2.668 ± 0.226
3.95AspSer: 3.95 ± 0.285
4.123AspThr: 4.123 ± 0.347
4.487AspVal: 4.487 ± 0.43
0.953AspTrp: 0.953 ± 0.13
3.222AspTyr: 3.222 ± 0.238
0.0AspXaa: 0.0 ± 0.0
Glu
3.621GluAla: 3.621 ± 0.226
0.554GluCys: 0.554 ± 0.121
4.123GluAsp: 4.123 ± 0.331
4.799GluGlu: 4.799 ± 0.529
3.257GluPhe: 3.257 ± 0.269
4.158GluGly: 4.158 ± 0.308
0.953GluHis: 0.953 ± 0.161
4.383GluIle: 4.383 ± 0.285
3.967GluLys: 3.967 ± 0.492
5.059GluLeu: 5.059 ± 0.388
1.525GluMet: 1.525 ± 0.254
3.326GluAsn: 3.326 ± 0.244
1.923GluPro: 1.923 ± 0.173
2.391GluGln: 2.391 ± 0.232
2.339GluArg: 2.339 ± 0.242
3.863GluSer: 3.863 ± 0.329
3.344GluThr: 3.344 ± 0.265
4.366GluVal: 4.366 ± 0.302
0.849GluTrp: 0.849 ± 0.143
2.962GluTyr: 2.962 ± 0.253
0.0GluXaa: 0.0 ± 0.0
Phe
2.564PheAla: 2.564 ± 0.22
0.433PheCys: 0.433 ± 0.086
3.621PheAsp: 3.621 ± 0.216
2.789PheGlu: 2.789 ± 0.209
1.559PhePhe: 1.559 ± 0.171
2.962PheGly: 2.962 ± 0.311
0.485PheHis: 0.485 ± 0.119
3.24PheIle: 3.24 ± 0.215
2.443PheLys: 2.443 ± 0.215
2.668PheLeu: 2.668 ± 0.268
1.039PheMet: 1.039 ± 0.169
3.032PheAsn: 3.032 ± 0.264
1.715PhePro: 1.715 ± 0.17
1.854PheGln: 1.854 ± 0.188
1.611PheArg: 1.611 ± 0.123
3.482PheSer: 3.482 ± 0.195
3.326PheThr: 3.326 ± 0.281
2.772PheVal: 2.772 ± 0.191
0.468PheTrp: 0.468 ± 0.106
1.594PheTyr: 1.594 ± 0.163
0.0PheXaa: 0.0 ± 0.0
Gly
5.509GlyAla: 5.509 ± 0.514
0.658GlyCys: 0.658 ± 0.121
5.578GlyAsp: 5.578 ± 0.468
4.106GlyGlu: 4.106 ± 0.262
3.17GlyPhe: 3.17 ± 0.294
7.346GlyGly: 7.346 ± 0.637
1.23GlyHis: 1.23 ± 0.167
4.608GlyIle: 4.608 ± 0.331
4.366GlyLys: 4.366 ± 0.514
4.608GlyLeu: 4.608 ± 0.291
1.628GlyMet: 1.628 ± 0.246
4.452GlyAsn: 4.452 ± 0.57
2.131GlyPro: 2.131 ± 0.272
2.98GlyGln: 2.98 ± 0.255
2.789GlyArg: 2.789 ± 0.207
5.561GlySer: 5.561 ± 0.679
6.895GlyThr: 6.895 ± 0.692
4.66GlyVal: 4.66 ± 0.325
1.126GlyTrp: 1.126 ± 0.133
3.482GlyTyr: 3.482 ± 0.298
0.0GlyXaa: 0.0 ± 0.0
His
0.814HisAla: 0.814 ± 0.108
0.208HisCys: 0.208 ± 0.067
1.247HisAsp: 1.247 ± 0.173
0.918HisGlu: 0.918 ± 0.165
0.797HisPhe: 0.797 ± 0.115
1.022HisGly: 1.022 ± 0.131
0.433HisHis: 0.433 ± 0.127
0.936HisIle: 0.936 ± 0.145
1.022HisLys: 1.022 ± 0.168
0.987HisLeu: 0.987 ± 0.195
0.329HisMet: 0.329 ± 0.071
0.797HisAsn: 0.797 ± 0.16
0.918HisPro: 0.918 ± 0.121
0.45HisGln: 0.45 ± 0.09
0.658HisArg: 0.658 ± 0.126
1.039HisSer: 1.039 ± 0.154
1.057HisThr: 1.057 ± 0.139
0.901HisVal: 0.901 ± 0.125
0.26HisTrp: 0.26 ± 0.091
0.918HisTyr: 0.918 ± 0.143
0.0HisXaa: 0.0 ± 0.0
Ile
4.175IleAla: 4.175 ± 0.28
0.502IleCys: 0.502 ± 0.104
5.041IleAsp: 5.041 ± 0.336
4.175IleGlu: 4.175 ± 0.299
2.148IlePhe: 2.148 ± 0.209
3.933IleGly: 3.933 ± 0.369
0.606IleHis: 0.606 ± 0.103
3.344IleIle: 3.344 ± 0.302
4.123IleLys: 4.123 ± 0.333
4.227IleLeu: 4.227 ± 0.286
1.161IleMet: 1.161 ± 0.161
4.071IleAsn: 4.071 ± 0.316
3.257IlePro: 3.257 ± 0.243
2.599IleGln: 2.599 ± 0.239
2.339IleArg: 2.339 ± 0.248
4.331IleSer: 4.331 ± 0.433
5.041IleThr: 5.041 ± 0.6
4.314IleVal: 4.314 ± 0.291
0.537IleTrp: 0.537 ± 0.093
2.183IleTyr: 2.183 ± 0.181
0.0IleXaa: 0.0 ± 0.0
Lys
3.95LysAla: 3.95 ± 0.549
0.52LysCys: 0.52 ± 0.1
3.673LysAsp: 3.673 ± 0.369
4.21LysGlu: 4.21 ± 0.46
3.188LysPhe: 3.188 ± 0.261
3.569LysGly: 3.569 ± 0.438
1.213LysHis: 1.213 ± 0.205
3.777LysIle: 3.777 ± 0.314
5.665LysLys: 5.665 ± 0.819
4.695LysLeu: 4.695 ± 0.415
1.802LysMet: 1.802 ± 0.261
3.205LysAsn: 3.205 ± 0.296
2.321LysPro: 2.321 ± 0.25
2.252LysGln: 2.252 ± 0.324
2.668LysArg: 2.668 ± 0.302
3.673LysSer: 3.673 ± 0.347
3.95LysThr: 3.95 ± 0.308
4.175LysVal: 4.175 ± 0.306
0.936LysTrp: 0.936 ± 0.132
2.928LysTyr: 2.928 ± 0.316
0.0LysXaa: 0.0 ± 0.0
Leu
4.678LeuAla: 4.678 ± 0.302
0.572LeuCys: 0.572 ± 0.14
5.596LeuAsp: 5.596 ± 0.349
4.418LeuGlu: 4.418 ± 0.315
2.98LeuPhe: 2.98 ± 0.254
4.574LeuGly: 4.574 ± 0.305
1.334LeuHis: 1.334 ± 0.191
3.829LeuIle: 3.829 ± 0.265
4.851LeuLys: 4.851 ± 0.498
5.093LeuLeu: 5.093 ± 0.32
1.178LeuMet: 1.178 ± 0.184
4.539LeuAsn: 4.539 ± 0.257
3.014LeuPro: 3.014 ± 0.332
3.066LeuGln: 3.066 ± 0.253
3.066LeuArg: 3.066 ± 0.28
4.92LeuSer: 4.92 ± 0.292
5.648LeuThr: 5.648 ± 0.421
4.21LeuVal: 4.21 ± 0.274
0.745LeuTrp: 0.745 ± 0.124
2.789LeuTyr: 2.789 ± 0.226
0.0LeuXaa: 0.0 ± 0.0
Met
1.68MetAla: 1.68 ± 0.27
0.191MetCys: 0.191 ± 0.059
1.403MetAsp: 1.403 ± 0.189
1.161MetGlu: 1.161 ± 0.201
0.866MetPhe: 0.866 ± 0.125
1.195MetGly: 1.195 ± 0.184
0.381MetHis: 0.381 ± 0.121
1.091MetIle: 1.091 ± 0.171
2.096MetLys: 2.096 ± 0.341
1.611MetLeu: 1.611 ± 0.183
0.537MetMet: 0.537 ± 0.098
1.109MetAsn: 1.109 ± 0.17
0.832MetPro: 0.832 ± 0.144
0.901MetGln: 0.901 ± 0.154
0.814MetArg: 0.814 ± 0.126
1.628MetSer: 1.628 ± 0.202
1.594MetThr: 1.594 ± 0.194
1.057MetVal: 1.057 ± 0.158
0.26MetTrp: 0.26 ± 0.072
0.658MetTyr: 0.658 ± 0.116
0.0MetXaa: 0.0 ± 0.0
Asn
4.487AsnAla: 4.487 ± 0.328
0.572AsnCys: 0.572 ± 0.116
3.014AsnAsp: 3.014 ± 0.285
3.43AsnGlu: 3.43 ± 0.245
2.807AsnPhe: 2.807 ± 0.231
4.868AsnGly: 4.868 ± 0.46
0.866AsnHis: 0.866 ± 0.113
3.794AsnIle: 3.794 ± 0.343
3.274AsnLys: 3.274 ± 0.328
4.885AsnLeu: 4.885 ± 0.445
1.005AsnMet: 1.005 ± 0.139
3.638AsnAsn: 3.638 ± 0.337
3.274AsnPro: 3.274 ± 0.252
2.079AsnGln: 2.079 ± 0.197
2.062AsnArg: 2.062 ± 0.174
3.863AsnSer: 3.863 ± 0.389
4.452AsnThr: 4.452 ± 0.388
3.967AsnVal: 3.967 ± 0.406
0.918AsnTrp: 0.918 ± 0.145
2.408AsnTyr: 2.408 ± 0.21
0.0AsnXaa: 0.0 ± 0.0
Pro
2.824ProAla: 2.824 ± 0.272
0.485ProCys: 0.485 ± 0.132
3.292ProAsp: 3.292 ± 0.28
2.962ProGlu: 2.962 ± 0.286
1.923ProPhe: 1.923 ± 0.185
3.17ProGly: 3.17 ± 0.277
0.97ProHis: 0.97 ± 0.15
2.425ProIle: 2.425 ± 0.242
2.079ProLys: 2.079 ± 0.245
2.373ProLeu: 2.373 ± 0.251
0.745ProMet: 0.745 ± 0.147
2.408ProAsn: 2.408 ± 0.204
1.836ProPro: 1.836 ± 0.256
1.23ProGln: 1.23 ± 0.16
1.594ProArg: 1.594 ± 0.193
3.188ProSer: 3.188 ± 0.205
3.032ProThr: 3.032 ± 0.223
2.737ProVal: 2.737 ± 0.236
0.554ProTrp: 0.554 ± 0.103
1.663ProTyr: 1.663 ± 0.189
0.0ProXaa: 0.0 ± 0.0
Gln
2.46GlnAla: 2.46 ± 0.242
0.26GlnCys: 0.26 ± 0.082
2.062GlnAsp: 2.062 ± 0.187
2.304GlnGlu: 2.304 ± 0.24
1.819GlnPhe: 1.819 ± 0.191
2.824GlnGly: 2.824 ± 0.228
0.71GlnHis: 0.71 ± 0.116
2.495GlnIle: 2.495 ± 0.191
2.737GlnLys: 2.737 ± 0.299
3.136GlnLeu: 3.136 ± 0.196
0.97GlnMet: 0.97 ± 0.159
2.044GlnAsn: 2.044 ± 0.196
1.369GlnPro: 1.369 ± 0.167
1.507GlnGln: 1.507 ± 0.15
1.75GlnArg: 1.75 ± 0.185
2.495GlnSer: 2.495 ± 0.219
2.373GlnThr: 2.373 ± 0.206
2.391GlnVal: 2.391 ± 0.185
0.624GlnTrp: 0.624 ± 0.133
1.958GlnTyr: 1.958 ± 0.194
0.0GlnXaa: 0.0 ± 0.0
Arg
2.356ArgAla: 2.356 ± 0.237
0.346ArgCys: 0.346 ± 0.074
2.72ArgAsp: 2.72 ± 0.239
2.252ArgGlu: 2.252 ± 0.263
1.975ArgPhe: 1.975 ± 0.197
2.824ArgGly: 2.824 ± 0.257
0.728ArgHis: 0.728 ± 0.134
2.807ArgIle: 2.807 ± 0.257
2.495ArgLys: 2.495 ± 0.272
2.928ArgLeu: 2.928 ± 0.361
0.832ArgMet: 0.832 ± 0.127
1.94ArgAsn: 1.94 ± 0.199
1.403ArgPro: 1.403 ± 0.154
1.767ArgGln: 1.767 ± 0.196
1.594ArgArg: 1.594 ± 0.242
2.356ArgSer: 2.356 ± 0.177
2.148ArgThr: 2.148 ± 0.274
3.17ArgVal: 3.17 ± 0.253
0.381ArgTrp: 0.381 ± 0.077
1.958ArgTyr: 1.958 ± 0.201
0.0ArgXaa: 0.0 ± 0.0
Ser
4.955SerAla: 4.955 ± 0.332
0.554SerCys: 0.554 ± 0.105
3.967SerAsp: 3.967 ± 0.237
3.673SerGlu: 3.673 ± 0.216
3.101SerPhe: 3.101 ± 0.185
6.705SerGly: 6.705 ± 0.737
0.866SerHis: 0.866 ± 0.111
4.331SerIle: 4.331 ± 0.358
3.586SerLys: 3.586 ± 0.374
4.539SerLeu: 4.539 ± 0.249
1.646SerMet: 1.646 ± 0.237
4.262SerAsn: 4.262 ± 0.338
2.46SerPro: 2.46 ± 0.247
2.477SerGln: 2.477 ± 0.21
2.616SerArg: 2.616 ± 0.193
5.63SerSer: 5.63 ± 0.55
4.504SerThr: 4.504 ± 0.431
4.608SerVal: 4.608 ± 0.308
0.78SerTrp: 0.78 ± 0.14
2.824SerTyr: 2.824 ± 0.182
0.0SerXaa: 0.0 ± 0.0
Thr
5.18ThrAla: 5.18 ± 0.499
0.537ThrCys: 0.537 ± 0.1
4.452ThrAsp: 4.452 ± 0.378
4.071ThrGlu: 4.071 ± 0.288
3.257ThrPhe: 3.257 ± 0.296
6.514ThrGly: 6.514 ± 0.596
0.918ThrHis: 0.918 ± 0.097
5.492ThrIle: 5.492 ± 0.644
3.707ThrLys: 3.707 ± 0.347
5.526ThrLeu: 5.526 ± 0.402
1.143ThrMet: 1.143 ± 0.17
4.522ThrAsn: 4.522 ± 0.528
2.945ThrPro: 2.945 ± 0.292
2.373ThrGln: 2.373 ± 0.187
2.321ThrArg: 2.321 ± 0.23
4.591ThrSer: 4.591 ± 0.463
5.353ThrThr: 5.353 ± 0.668
5.665ThrVal: 5.665 ± 0.7
0.78ThrTrp: 0.78 ± 0.121
2.893ThrTyr: 2.893 ± 0.22
0.0ThrXaa: 0.0 ± 0.0
Val
4.591ValAla: 4.591 ± 0.284
0.433ValCys: 0.433 ± 0.106
5.232ValAsp: 5.232 ± 0.429
4.227ValGlu: 4.227 ± 0.341
2.564ValPhe: 2.564 ± 0.212
4.834ValGly: 4.834 ± 0.464
0.97ValHis: 0.97 ± 0.16
4.071ValIle: 4.071 ± 0.344
3.915ValLys: 3.915 ± 0.307
4.348ValLeu: 4.348 ± 0.28
1.178ValMet: 1.178 ± 0.187
4.331ValAsn: 4.331 ± 0.379
2.685ValPro: 2.685 ± 0.221
2.512ValGln: 2.512 ± 0.199
2.495ValArg: 2.495 ± 0.236
4.591ValSer: 4.591 ± 0.373
5.405ValThr: 5.405 ± 0.497
4.504ValVal: 4.504 ± 0.353
0.71ValTrp: 0.71 ± 0.111
2.772ValTyr: 2.772 ± 0.209
0.0ValXaa: 0.0 ± 0.0
Trp
0.728TrpAla: 0.728 ± 0.111
0.191TrpCys: 0.191 ± 0.065
0.901TrpAsp: 0.901 ± 0.121
0.693TrpGlu: 0.693 ± 0.118
0.433TrpPhe: 0.433 ± 0.1
0.814TrpGly: 0.814 ± 0.118
0.225TrpHis: 0.225 ± 0.074
0.762TrpIle: 0.762 ± 0.108
0.987TrpLys: 0.987 ± 0.161
0.728TrpLeu: 0.728 ± 0.137
0.381TrpMet: 0.381 ± 0.083
0.901TrpAsn: 0.901 ± 0.148
0.364TrpPro: 0.364 ± 0.068
0.606TrpGln: 0.606 ± 0.11
0.624TrpArg: 0.624 ± 0.136
0.884TrpSer: 0.884 ± 0.117
0.658TrpThr: 0.658 ± 0.1
0.884TrpVal: 0.884 ± 0.149
0.156TrpTrp: 0.156 ± 0.045
0.364TrpTyr: 0.364 ± 0.088
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.373TyrAla: 2.373 ± 0.213
0.589TyrCys: 0.589 ± 0.096
3.274TyrAsp: 3.274 ± 0.253
2.737TyrGlu: 2.737 ± 0.244
1.594TyrPhe: 1.594 ± 0.17
2.564TyrGly: 2.564 ± 0.193
0.658TyrHis: 0.658 ± 0.105
2.287TyrIle: 2.287 ± 0.235
2.841TyrLys: 2.841 ± 0.314
3.153TyrLeu: 3.153 ± 0.291
0.762TyrMet: 0.762 ± 0.125
3.101TyrAsn: 3.101 ± 0.247
2.062TyrPro: 2.062 ± 0.195
1.767TyrGln: 1.767 ± 0.216
1.923TyrArg: 1.923 ± 0.228
2.408TyrSer: 2.408 ± 0.226
2.911TyrThr: 2.911 ± 0.328
2.98TyrVal: 2.98 ± 0.175
0.329TyrTrp: 0.329 ± 0.091
2.01TyrTyr: 2.01 ± 0.203
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 220 proteins (57723 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski