Hamiltosporidium tvaerminnensis
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5820 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q9LPJ8|A0A4Q9LPJ8_9MICR Uncharacterized protein OS=Hamiltosporidium tvaerminnensis OX=1176355 GN=CWI38_1824p0010 PE=4 SV=1
MM1 pKa = 7.44 IISWLSLYY9 pKa = 11.23 LLFTYY14 pKa = 10.75 VMIVKK19 pKa = 9.75 PNEE22 pKa = 3.92 SDD24 pKa = 4.2 SIQAPGSYY32 pKa = 9.63 IYY34 pKa = 10.19 TLYY37 pKa = 11.39 GNLNTDD43 pKa = 3.63 EE44 pKa = 4.96 GPNSGSGFTRR54 pKa = 11.84 TNSGGNGMDD63 pKa = 3.44 NSPVNGFNAHH73 pKa = 6.34 SFTNNYY79 pKa = 9.23 FPLDD83 pKa = 3.79 NPSVIGLWFGLNDD96 pKa = 3.6 AFEE99 pKa = 4.55 TVAPVSMDD107 pKa = 3.16 QTNGTNDD114 pKa = 3.54 LADD117 pKa = 3.72 YY118 pKa = 8.83 TPNDD122 pKa = 4.2 SITFGNNGIEE132 pKa = 4.22 EE133 pKa = 4.51 SEE135 pKa = 5.05 IMDD138 pKa = 4.1 QTNMDD143 pKa = 4.02 TNSTYY148 pKa = 10.76 QSTMFCGNGDD158 pKa = 3.85 SVALGSAAMEE168 pKa = 3.89 QNIIDD173 pKa = 4.0 EE174 pKa = 4.75 DD175 pKa = 4.52 RR176 pKa = 11.84 NSDD179 pKa = 3.69 MLVEE183 pKa = 4.46 NQNHH187 pKa = 6.84 DD188 pKa = 3.48 NPLFLGSTVDD198 pKa = 3.37 EE199 pKa = 4.16 ATKK202 pKa = 10.62 NGRR205 pKa = 11.84 QLYY208 pKa = 6.07 TTSLSDD214 pKa = 3.81 KK215 pKa = 10.83 DD216 pKa = 4.65 DD217 pKa = 4.51 LFFLDD222 pKa = 4.37 EE223 pKa = 4.99 LDD225 pKa = 4.61 LVFISDD231 pKa = 4.79 AIEE234 pKa = 4.22 SKK236 pKa = 10.63 IKK238 pKa = 10.67
Molecular weight: 26.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.617
IPC_protein 3.63
Toseland 3.401
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.795
Wikipedia 3.592
Rodwell 3.465
Grimsley 3.312
Solomon 3.617
Lehninger 3.579
Nozaki 3.757
DTASelect 4.012
Thurlkill 3.465
EMBOSS 3.592
Sillero 3.757
Patrickios 1.036
IPC_peptide 3.617
IPC2_peptide 3.732
IPC2.peptide.svr19 3.712
Protein with the highest isoelectric point:
>tr|A0A4Q9KQL9|A0A4Q9KQL9_9MICR Putative subunit 2 of mRNA decapping complex OS=Hamiltosporidium tvaerminnensis OX=1176355 GN=CWI37_2600p0020 PE=3 SV=1
MM1 pKa = 7.1 NRR3 pKa = 11.84 KK4 pKa = 9.08 KK5 pKa = 10.83 LFLWIIQLTIILNFIKK21 pKa = 10.4 RR22 pKa = 11.84 VSKK25 pKa = 11.06 VNAGYY30 pKa = 10.07 SRR32 pKa = 11.84 RR33 pKa = 11.84 SYY35 pKa = 10.43 SSRR38 pKa = 11.84 NIRR41 pKa = 11.84 RR42 pKa = 11.84 SSGCSGGRR50 pKa = 11.84 QGGGGCKK57 pKa = 10.08 GGSCSGGRR65 pKa = 11.84 QGGGGCKK72 pKa = 10.08 GGSCSGGRR80 pKa = 11.84 QGGGGCKK87 pKa = 10.08 GGSCSGGRR95 pKa = 11.84 QGGGGCKK102 pKa = 10.08 GGSCSGGRR110 pKa = 11.84 QGGGGGCSSCGGGGCSSCGGRR131 pKa = 11.84 NPRR134 pKa = 4.07
Molecular weight: 12.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.604
IPC_protein 10.189
Toseland 10.76
ProMoST 10.643
Dawson 10.833
Bjellqvist 10.57
Wikipedia 11.008
Rodwell 11.096
Grimsley 10.862
Solomon 10.95
Lehninger 10.935
Nozaki 10.833
DTASelect 10.526
Thurlkill 10.76
EMBOSS 11.155
Sillero 10.789
Patrickios 10.847
IPC_peptide 10.965
IPC2_peptide 10.131
IPC2.peptide.svr19 8.705
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5820
0
5820
2114574
46
3235
363.3
42.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
2.597 ± 0.028
1.944 ± 0.013
5.194 ± 0.021
7.601 ± 0.041
5.966 ± 0.04
3.606 ± 0.035
1.71 ± 0.012
9.726 ± 0.035
9.811 ± 0.04
9.523 ± 0.034
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.934 ± 0.014
8.671 ± 0.056
2.64 ± 0.024
2.48 ± 0.017
3.785 ± 0.023
7.825 ± 0.031
5.062 ± 0.03
4.813 ± 0.029
0.528 ± 0.006
4.584 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here